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Gene information
General information
TC010825 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 5845382..5852609
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010825
    iBeetle screen iB_01740
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010825 RA
      MVSRRRILS RSRDDLHMD STFQPPEDE EDVWYQKDK 
      LYKDHIQEV LDKWTQIDD EIWAKVIVL ERNRRVAKA 
      YARAPVLTV NGSDDGFDG FRIGLCGFD NPMRDQKTE 
      EFKRHIGHG VKIKMDDAG NILIKRVSK CNVYIKNTG 
      QDDENAIGN EILKLPNCA LEPEKPVKL FDMKKFQSN 
      VNRELRRAY PDRRRLECQ CLSAIAFVK SEPELLECP 
      IWVLIINVV AMDMLKSKL PPVQRVMDI KNRPRIPIP 
      DEDPYSVAG NGSGSSGSS GFAGAGSVA ATREQLLLQ 
      SQQHAQRRS EKPPKLPPR ENMYPHDIP KPDYDDHEH 
      DNSRLKPFP SSRGKEKSK DSKKYDDPY YCGLRARVP 
      NFVAKSKAK ESVSSKRFS ISQQAPPPL PVMHQQHVN 
      SHHNMHQHS MWHTRSFES GIDSDVVES PYNQIYGRL 
      PIPTRAYIP SQSRTMYIG EWD
    CDS sequence
    • >TC010825 RA
      atggtttct cgtcgccgg attctttca agatcgcga 
      gatgatttg cacatggac tccactttc cagcccccg 
      gaagacgag gaagacgtt tggtaccaa aaagacaag 
      ctttacaag gaccacatc caagaagtg ctcgataag 
      tggacacag atagatgac gagatttgg gccaaagtg 
      attgtttta gagcgaaac cggagggtg gctaaggcg 
      tacgccaga gccccggtt ttgacagta aatgggtcc 
      gatgacggg tttgatggt tttcggatt ggtttgtgc 
      ggctttgac aacccaatg cgagaccag aagacggag 
      gaattcaaa agacacata ggacatgga gtgaaaata 
      aaaatggac gacgcgggc aatattcta ataaaacgg 
      gtatcaaag tgtaacgtt tacataaaa aatacgggc 
      caagacgac gagaacgcg atagggaat gaaattttg 
      aaattacct aattgtgcg ctagagccg gaaaagccc 
      gtaaagctg ttcgatatg aaaaaattc cagtcaaac 
      gtaaatcgt gaactacgg cgtgcttat cccgaccgg 
      cgaagatta gaatgtcag tgtttgagt gcgatagct 
      tttgtaaaa tcggagccg gagttgctg gagtgccct 
      atttgggtg ctcattata aacgtcgtc gccatggac 
      atgctaaag tcgaaactt cctccagtc caacgggta 
      atggacatc aagaacaga ccacgcatt ccaattcca 
      gatgaagat ccctatagc gttgcaggt aacggtagc 
      ggatcgtcg ggtagttca ggtttcgcg ggggcgggt 
      agtgtggca gccactaga gaacaactc cttttgcag 
      agccaacag cacgcacaa cggcggagc gagaaacca 
      cctaaacta ccaccccgc gagaatatg tatccccat 
      gacatacct aagcccgat tatgacgac cacgagcac 
      gacaacagc cgtctgaag ccgttcccg tcctctcgg 
      gggaaggag aagtcgaaa gacagcaaa aaatacgac 
      gacccttat tactgcgga ttaagggcc cgtgttccg 
      aattttgtg gcgaagtcg aaagcgaag gagtccgtt 
      tcgtcgaag agattttcg ataagtcag caggcgccg 
      ccgcccctt cctgtgatg caccagcaa cacgtcaac 
      tcgcatcat aacatgcac cagcattca atgtggcat 
      acgaggagt tttgagagc ggaatcgac tcggatgta 
      gtcgagtcg ccctacaac caaatttac ggacggtta 
      cccataccc acgagggcg tatattcca agtcagtcg 
      cgtaccatg tatatcgga gaatgggac taa
    mRNA sequence
    • >TC010825 RA
      agacgagtg tgcgcacgc cgctgtcga aagcgcgct 
      ctaaaccga atgtttcct tcattctat tctttggta 
      gatgtgtgc tgtagcacc gcgacggtt ttgtgcact 
      caattcgtc gcgagtgac aagatttgt gcccgaaat 
      ggatttagt tcgcgaatt ttgctagtg tagtggttc 
      tctagttcc tactcctct ctttggatt actctttct 
      tgtccgtac acgccctta gtttttgac taactttag 
      ctctgccac tgcctgaaa cgcctgaaa tggtttctc 
      gtcgccgga ttctttcaa gatcgcgag atgatttgc 
      acatggact ccactttcc agcccccgg aagacgagg 
      aagacgttt ggtaccaaa aagacaagc tttacaagg 
      accacatcc aagaagtgc tcgataagt ggacacaga 
      tagatgacg agatttggg ccaaagtga ttgttttag 
      agcgaaacc ggagggtgg ctaaggcgt acgccagag 
      ccccggttt tgacagtaa atgggtccg atgacgggt 
      ttgatggtt ttcggattg gtttgtgcg gctttgaca 
      acccaatgc gagaccaga agacggagg aattcaaaa 
      gacacatag gacatggag tgaaaataa aaatggacg 
      acgcgggca atattctaa taaaacggg tatcaaagt 
      gtaacgttt acataaaaa atacgggcc aagacgacg 
      agaacgcga tagggaatg aaattttga aattaccta 
      attgtgcgc tagagccgg aaaagcccg taaagctgt 
      tcgatatga aaaaattcc agtcaaacg taaatcgtg 
      aactacggc gtgcttatc ccgaccggc gaagattag 
      aatgtcagt gtttgagtg cgatagctt ttgtaaaat 
      cggagccgg agttgctgg agtgcccta tttgggtgc 
      tcattataa acgtcgtcg ccatggaca tgctaaagt 
      cgaaacttc ctccagtcc aacgggtaa tggacatca 
      agaacagac cacgcattc caattccag atgaagatc 
      cctatagcg ttgcaggta acggtagcg gatcgtcgg 
      gtagttcag gtttcgcgg gggcgggta gtgtggcag 
      ccactagag aacaactcc ttttgcaga gccaacagc 
      acgcacaac ggcggagcg agaaaccac ctaaactac 
      caccccgcg agaatatgt atccccatg acataccta 
      agcccgatt atgacgacc acgagcacg acaacagcc 
      gtctgaagc cgttcccgt cctctcggg ggaaggaga 
      agtcgaaag acagcaaaa aatacgacg acccttatt 
      actgcggat taagggccc gtgttccga attttgtgg 
      cgaagtcga aagcgaagg agtccgttt cgtcgaaga 
      gattttcga taagtcagc aggcgccgc cgccccttc 
      ctgtgatgc accagcaac acgtcaact cgcatcata 
      acatgcacc agcattcaa tgtggcata cgaggagtt 
      ttgagagcg gaatcgact cggatgtag tcgagtcgc 
      cctacaacc aaatttacg gacggttac ccataccca 
      cgagggcgt atattccaa gtcagtcgc gtaccatgt 
      atatcggag aatgggact aatattgtg tacataact 
      ggacatttt ccaattgaa agactttgt aaatttcat 
      tatttaaca ccactatgt ctattcgat actgccact 
      actgtttat agacttttt acagcaaat ttatactat 
      gagtgctat tttatactt gtaaacggc tttgtaaca 
      tattattta cagaatatt taatttaat ttagtgaaa 
      ttttagtgt tctgtcaaa tactcagta aataaatgt 
      acctatagt attataaca acggatatt aaatcagga 
      tgtgattat atacgtcat agtcatgtt attataata 
      tatagatag atatatgct atgttgata ggatacgtt 
      tttgtaaag taaacttat aaattgtat attgtatgt 
      ttttgtaat tactgcaat aaacggtga tggatgta