Status
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Gene information
General information
TC010820 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6075846..6080953
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010820
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01739

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010820 RA
      MEASYCLCG QPYDPNIFM IQCDACKDW FHSSCCNFQ 
      EHLAIEIDK YHCPKCAPI FGPSIYKPY RNWHRHDYW 
      DEDATTKPV QTGTGVFIR ELLARHFAS EGEVVVHMR 
      GQQITETLL QQQGFNNPI VVDGKDGLD MTLPPDNFS 
      LYDVESYVG GDMEIDVID VTRQCNIKM KLRDYVEYY 
      NSLNRTRVF NVVSLEFSK TALAPLVEA PSIARKLDW 
      ANIVWPRDF PLERPQVQK YCLMSVKDS YTDFHIDFG 
      GTSVWYHVL RGEKIFYLI RPTPANLTL YQQWMTSSN 
      QSETFFGDQ VDRCYKCIL RQGQTMMIP TGWIHAVLT 
      PIDSLVFGG NFLQSLNIP MQLAVYDIE KKVKTDAKY 
      RYPNFELIN WFAASHLLK KISELNSED RKCPNHLLV 
      GLKTLLITL KQWNSEKDY NTSVRDQIP YTINPVQLL 
      KDMSREIRH AERIINQLN PPKPERESK RKRRRPINR 
      DFVDYSLSP KSSAQDFIS ETKDYARQP PLKLSLPKP 
      VTYPYDRPN FMEQRINVE EQSWLSPQF SRPEQTTAI 
      HRGGAVLKF KLGSKDILP HSHADDIPE EQFDFKSVY 
      DFHDESDRD EDCFNFTID ENPPPKKSK KQSSAKFKF 
      PANFDEKAK KGKDPVIDI DGDLPKNGI ESLLKASAL 
      TTTDNNGRT SPSTQEAIA GMLSISQTY LQSGKANAR 
      SVRKYSTSP PLEDGNLNN VHQDEDYIY PSLDNSDDE 
      DIHIFKPRG RSKIDEAWN PKARVGPLL PKMNRPARE 
      GTKKQSVEK VLEAAAARR ANETPEKAP KRQYRRKKI 
      KQKLEIKSP ASNTVAVGS SSSASTPKP RKGMKTVKQ 
      RLGKILKIH KMMH
    CDS sequence
    • >TC010820 RA
      atggaagct tcatattgt ttgtgtggt cagccttac 
      gatcctaat atatttatg atacaatgt gatgcctgc 
      aaagactgg ttccacagc agttgttgt aactttcaa 
      gaacattta gccatagaa atcgacaag taccactgt 
      cccaagtgc gctccaatc ttcgggccc tcgatttat 
      aaaccgtac cgtaactgg catagacat gactattgg 
      gacgaggac gccaccacc aagcccgtg cagacgggc 
      actggggtg ttcatcaga gagctgctg gccaggcat 
      ttcgcaagc gaaggcgag gttgtcgtg cacatgaga 
      ggtcagcag attacggaa acgctgctt caacagcaa 
      ggtttcaac aaccccatt gtggtcgac ggcaaggac 
      ggcctggac atgaccctc ccccctgac aacttcagt 
      ctctacgac gtggagtcg tacgtgggc ggggacatg 
      gaaatcgac gtaatcgac gttacgaga caatgtaac 
      atcaagatg aagctgcgg gattacgtc gaatactac 
      aatagtctg aacagaacg cgcgtcttc aacgtggtc 
      agtttggag ttctcaaag acagcctta gcgcctttg 
      gtggaagcc ccctcaatc gcccgcaag ctggactgg 
      gcgaacatc gtctggccc cgcgatttc cccctcgag 
      cggccccaa gtccagaaa tactgtctc atgagtgtc 
      aaggactcc tacaccgat tttcacatc gacttcggg 
      ggcaccagt gtttggtac cacgtccta aggggcgag 
      aaaatcttc tatctcata agaccaacc cccgccaat 
      ttaaccctc taccaacag tggatgact tcaagcaac 
      cagagcgaa acgtttttc ggcgatcaa gtcgatcgg 
      tgctacaaa tgcattctg aggcaagga caaacgatg 
      atgatcccc acagggtgg attcatgcc gttttaaca 
      ccgattgat tcactcgtt ttcggggga aatttccta 
      caaagcttg aacataccc atgcagctg gctgtttat 
      gatatagag aaaaaggtg aaaacggac gccaagtac 
      aggtacccg aactttgaa ctaatcaat tggtttgcc 
      gcctcgcat ttgctaaaa aaaatctcg gaactcaac 
      agtgaggac aggaaatgt cccaatcat ttactggtc 
      ggacttaag accttgcta ataactttg aaacagtgg 
      aattcggag aaagactac aacacaagt gtgagggat 
      caaatcccg tacactata aatccagtc cagttgttg 
      aaagacatg tcgcgcgaa atacgacat gcggagcga 
      attattaat cagctaaat ccgccgaaa ccggaacgt 
      gagtcaaaa cggaagagg cgacgacca ataaaccgg 
      gatttcgtc gattattcc ctctcgcct aaatcctct 
      gctcaagat tttatatca gaaacgaaa gattacgcc 
      cgccaaccc cccctgaaa ttatcgctt ccgaaacca 
      gtgacttat ccatacgat cgaccaaat tttatggag 
      caacgaatt aatgtagag gagcaaagt tggctatcg 
      ccccaattt tcgcgtccc gaacaaaca acagccata 
      catcggggc ggtgccgtc ttaaaattc aaattagga 
      tctaaggat attctgcca cacagccac gctgacgac 
      attccagaa gaacagttt gatttcaaa tctgtatat 
      gatttccat gatgaaagt gatagagat gaagactgc 
      tttaatttt acaatcgac gagaatccg ccgccaaaa 
      aaaagtaaa aagcaaagt tcggccaaa ttcaaattt 
      ccagcaaat ttcgacgag aaggcgaaa aaagggaaa 
      gatccggtg atagacatc gacggtgat ttgccaaaa 
      aacgggatc gaatctcta ctaaaagcc tctgctctt 
      acaacaact gacaacaac ggacgaact tcgccttct 
      acccaagag gcgatcgct ggaatgctc tcaatcagc 
      caaacgtac ctacaatcg ggcaaagcc aacgccaga 
      tctgttcgc aaatacagc actagtccg cctttagaa 
      gacggaaac ctcaataac gtccaccag gacgaagat 
      tacatttac ccatccttg gataattcc gacgacgag 
      gatatccac atattcaaa ccccgaggg cgttcgaaa 
      atcgacgag gcctggaac ccgaaggcc agagtcggg 
      cctttgctg ccgaaaatg aacagaccg gcccgcgaa 
      gggacaaaa aaacagtca gttgagaag gtgctagaa 
      gccgctgct gcgagaagg gccaacgaa acacccgaa 
      aaagcgccg aaacgacaa taccggcgg aaaaaaatc 
      aagcaaaaa ctggagatt aaaagcccg gcttccaac 
      acagtcgcg gtgggaagc agctcttca gcctcgact 
      cccaagccg cggaaaggc atgaagacg gtcaaacaa 
      cggctgggg aagatcttg aaaatccac aaaatgatg 
      cattga
    mRNA sequence
    • >TC010820 RA
      acagtaaac aacgcgtta ctgtagaaa cctctaact 
      tccttatca tttgtaaaa taatgacat aatcacaat 
      gttagtatg gttgttagt ttggttagt ttacacaag 
      cagtttaca aaataatat tttataatg gaagcttca 
      tattgtttg tgtggtcag ccttacgat cctaatata 
      tttatgata caatgtgat gcctgcaaa gactggttc 
      cacagcagt tgttgtaac tttcaagaa catttagcc 
      atagaaatc gacaagtac cactgtccc aagtgcgct 
      ccaatcttc gggccctcg atttataaa ccgtaccgt 
      aactggcat agacatgac tattgggac gaggacgcc 
      accaccaag cccgtgcag acgggcact ggggtgttc 
      atcagagag ctgctggcc aggcatttc gcaagcgaa 
      ggcgaggtt gtcgtgcac atgagaggt cagcagatt 
      acggaaacg ctgcttcaa cagcaaggt ttcaacaac 
      cccattgtg gtcgacggc aaggacggc ctggacatg 
      accctcccc cctgacaac ttcagtctc tacgacgtg 
      gagtcgtac gtgggcggg gacatggaa atcgacgta 
      atcgacgtt acgagacaa tgtaacatc aagatgaag 
      ctgcgggat tacgtcgaa tactacaat agtctgaac 
      agaacgcgc gtcttcaac gtggtcagt ttggagttc 
      tcaaagaca gccttagcg cctttggtg gaagccccc 
      tcaatcgcc cgcaagctg gactgggcg aacatcgtc 
      tggccccgc gatttcccc ctcgagcgg ccccaagtc 
      cagaaatac tgtctcatg agtgtcaag gactcctac 
      accgatttt cacatcgac ttcgggggc accagtgtt 
      tggtaccac gtcctaagg ggcgagaaa atcttctat 
      ctcataaga ccaaccccc gccaattta accctctac 
      caacagtgg atgacttca agcaaccag agcgaaacg 
      tttttcggc gatcaagtc gatcggtgc tacaaatgc 
      attctgagg caaggacaa acgatgatg atccccaca 
      gggtggatt catgccgtt ttaacaccg attgattca 
      ctcgttttc gggggaaat ttcctacaa agcttgaac 
      atacccatg cagctggct gtttatgat atagagaaa 
      aaggtgaaa acggacgcc aagtacagg tacccgaac 
      tttgaacta atcaattgg tttgccgcc tcgcatttg 
      ctaaaaaaa atctcggaa ctcaacagt gaggacagg 
      aaatgtccc aatcattta ctggtcgga cttaagacc 
      ttgctaata actttgaaa cagtggaat tcggagaaa 
      gactacaac acaagtgtg agggatcaa atcccgtac 
      actataaat ccagtccag ttgttgaaa gacatgtcg 
      cgcgaaata cgacatgcg gagcgaatt attaatcag 
      ctaaatccg ccgaaaccg gaacgtgag tcaaaacgg 
      aagaggcga cgaccaata aaccgggat ttcgtcgat 
      tattccctc tcgcctaaa tcctctgct caagatttt 
      atatcagaa acgaaagat tacgcccgc caacccccc 
      ctgaaatta tcgcttccg aaaccagtg acttatcca 
      tacgatcga ccaaatttt atggagcaa cgaattaat 
      gtagaggag caaagttgg ctatcgccc caattttcg 
      cgtcccgaa caaacaaca gccatacat cggggcggt 
      gccgtctta aaattcaaa ttaggatct aaggatatt 
      ctgccacac agccacgct gacgacatt ccagaagaa 
      cagtttgat ttcaaatct gtatatgat ttccatgat 
      gaaagtgat agagatgaa gactgcttt aattttaca 
      atcgacgag aatccgccg ccaaaaaaa agtaaaaag 
      caaagttcg gccaaattc aaatttcca gcaaatttc 
      gacgagaag gcgaaaaaa gggaaagat ccggtgata 
      gacatcgac ggtgatttg ccaaaaaac gggatcgaa 
      tctctacta aaagcctct gctcttaca acaactgac 
      aacaacgga cgaacttcg ccttctacc caagaggcg 
      atcgctgga atgctctca atcagccaa acgtaccta 
      caatcgggc aaagccaac gccagatct gttcgcaaa 
      tacagcact agtccgcct ttagaagac ggaaacctc 
      aataacgtc caccaggac gaagattac atttaccca 
      tccttggat aattccgac gacgaggat atccacata 
      ttcaaaccc cgagggcgt tcgaaaatc gacgaggcc 
      tggaacccg aaggccaga gtcgggcct ttgctgccg 
      aaaatgaac agaccggcc cgcgaaggg acaaaaaaa 
      cagtcagtt gagaaggtg ctagaagcc gctgctgcg 
      agaagggcc aacgaaaca cccgaaaaa gcgccgaaa 
      cgacaatac cggcggaaa aaaatcaag caaaaactg 
      gagattaaa agcccggct tccaacaca gtcgcggtg 
      ggaagcagc tcttcagcc tcgactccc aagccgcgg 
      aaaggcatg aagacggtc aaacaacgg ctggggaag 
      atcttgaaa atccacaaa atgatgcat tgataataa 
      ctctgtgtt agtttgatt tggttgatt cttacgtgt 
      tcaggaagg tccgcagag aactattat ttattgtta 
      tcgttacgt ttcgttagg caaatttac tccgtcata 
      gttaatttt tcctactgg tccataaat tgtggtttc 
      gttttcaat actcagtgc catggtggt actagctgt 
      tcgtcagta ttttattct tatggtaaa gtaacatta 
      attaggtcc tatggtatt gtattggtt agttttgtt 
      ggtattgta tatatcgag tctcagtta atttaatta 
      tgtaaaagt attgaacga taatcaggg aatttttat 
      ttgtaatta ttcacgatg taaatattg ttgatttct 
      gtgatcgtg caaatcaat ttgcatttt aacatagtt 
      tcgaattga tgcgtcctt atccactca cttgcgcgg 
      taagtggat aatcacgga ttcatgaat taattaaaa 
      atgaattgt tattaagat aataca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY