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Gene information
General information
TC010817 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 5750270..5753827
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010817
    iBeetle screen iB_01738
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010817 RA
      MRVLEIFVA FIFFINSSL CQDNAGTNL APALQQCYN 
      TTQFAIRDY RLPSDMTVL IDIIRRIED GNAFQDARE 
      LSFEILHRF RQDGIRRTT KDLDSTQGI PFSPKGKES 
      FKNRVQLKK ITPGNAYNF NNDSLTPLQ ACSLHFMIS 
      STIDLHELA DDTVCNKIE HYTSRVEKR ETEFVEADQ 
      PKNRCPVES GVVYTKWGE VKAGILFAG IAAGFQPNS 
      VPVVNGSVD SAYASTLAG ELSEVALYE VGKPRVVGN 
      KGSWNTTID RKYYFLANS DKDHLYLTD AEIRGSLDG 
      LFLALNIQK WKSQSSDIK ISQILDLYY GRGVFNDTA 
      RVCNRNKLM RDLVASDKL VQQTSFFNY LLDQESQYD 
      YTPIKQNFP TFAQYAVES LRSYLDTNL KDRECSAEE 
      DVIERVSSH LLIFVDTTW TYQTIQPII SHILKNIDV 
      NKYETSYTI YDGTNCNTI VNRSSSILD FYDQYNVTQ 
      HQKYPTGFD YSKAIQTAE VAFKAKLDN ATDLTGQSM 
      IVLMLPHNK PTSSQSSYV QSEKEDFKS VLPDVRIIV 
      AGSESKESY SDIATNSNT DVFTISEGS DETLRHAAE 
      NIVARLKKI PRSVVNPKC GSSFETDET SSFEITQYV 
      QPTTVNYHK ISPNYFKDG QTLKIRGQG AGTIAVCIS 
      RTDANPRAN ATGANCTNI ESNELTRDI STFCTDESL 
      TDCSPIYIS VQGMTSNVR CTDKSCRFP NNIKYTISL 
      ENVKCSSCG KLLINNLMI FVMLVVYCF TSKYLN
    CDS sequence
    • >TC010817 RA
      atgagagtg ctagaaatt tttgtagcg tttatattt 
      tttataaat tcgagttta tgtcaagac aatgccggt 
      acaaatctc gccccggcg ttgcaacag tgttacaat 
      accacccag tttgccata agggactac agattacca 
      tcagacatg accgtccta attgacatc attcgaaga 
      atcgaagat ggaaacgca ttccaggac gcccgagaa 
      ctttctttc gaaattctc cacaggttt cggcaggac 
      gggatccgg aggaccacg aaggacctg gacagcacc 
      cagggcatc cccttcagc cccaagggg aaggagtct 
      ttcaagaac agggtccag ttgaagaag ataacgccc 
      ggaaacgcg tacaatttc aataacgac agcttaacg 
      ccgctgcaa gcctgttcg ctccatttt atgatttcg 
      agtacgatt gatttgcac gaattagcc gacgatacc 
      gtgtgtaac aaaatcgaa cattacaca tcccgggtg 
      gaaaaacga gaaactgag tttgtggaa gccgaccaa 
      ccgaaaaat cggtgcccc gttgaaagc ggcgttgtt 
      tacacgaaa tggggcgaa gtcaaagct gggattttg 
      tttgccggg attgcggcc ggttttcag ccaaattct 
      gtgcctgta gtcaatggt agcgtcgac tcggcctat 
      gctagcaca cttgcaggg gaactgtcg gaggttgct 
      ttgtacgaa gtagggaaa ccacgagtt gtcggaaat 
      aagggtagc tggaacacg accattgac cggaaatac 
      tactttttg gcaaattcg gataaggac cacttgtat 
      ctgactgat gccgagatt cgtgggagc ctcgatggc 
      ttgtttctg gctcttaac atccaaaag tggaagtca 
      cagtcgagt gacatcaaa atttcacaa attttagat 
      ctttattac gggagaggg gtttttaac gatacggca 
      cgagtgtgc aaccggaat aagttaatg cgagattta 
      gtagcaagt gacaaactt gtacaacaa acttccttt 
      tttaattac ttgctagat caagaatcg cagtacgat 
      tacacaccc atcaagcaa aacttcccc acgtttgct 
      cagtacgct gtcgagagc ttgagatct tatttagat 
      accaatctg aaggatcga gagtgttcg gcggaagaa 
      gatgtaatt gagcgagtg tcctcacat ttattaatt 
      tttgtcgat acgacctgg acttatcaa acgattcag 
      ccgattatt tcccacatt ttgaaaaac atcgatgtc 
      aataaatac gaaacttct tatacaatt tacgacggt 
      accaactgt aacacaatt gtcaataga tcgagcagt 
      attttggac ttttatgac cagtataac gtcacccaa 
      caccaaaaa tatcccacc gggtttgac tatagcaaa 
      gctatacaa acggcggaa gtagctttt aaggcgaaa 
      ttagacaac gccaccgat ttgaccgga cagtcgatg 
      attgtccta atgctgcca cataacaaa ccaacttca 
      agccaaagc agttatgtt caaagtgaa aaagaggat 
      ttcaaaagc gttttgccc gacgttaga attattgtt 
      gctggatca gaaagtaaa gagtcgtat tccgacatc 
      gccacaaac tccaacaca gacgttttc acgatttct 
      gaaggtagc gacgagaca ttgcgacat gcagccgaa 
      aatatcgtt gccagattg aaaaaaata ccacgaagt 
      gttgtcaac cctaaatgt ggttcctct ttcgaaact 
      gacgaaacc agcagtttt gaaataaca caatacgtt 
      caacccacc acagttaat tatcacaaa atttcgccg 
      aattacttc aaggatgga caaacgctg aagattcgg 
      ggacaaggt gctggcact atcgctgtg tgtatatcg 
      agaactgat gccaatccg agggcaaat gccactggg 
      gccaattgt acaaacatc gaatccaac gagttgact 
      agagacatc agtactttt tgcaccgat gagtcccta 
      accgattgc tctccgatt tatatttct gtccaagga 
      atgacatcg aacgttcgt tgcacagac aaatcttgc 
      cgattcccc aacaacatc aaatacaca atatctcta 
      gaaaatgtc aagtgttca agctgcggc aaactgctt 
      ataaataat ttgatgatt ttcgtcatg cttgttgtt 
      tactgcttt acttctaag tacctaaat tag
    mRNA sequence
    • >TC010817 RA
      cgccagtgc cccgtttaa ttttacagg tgtatttca 
      ctacagtac atgtgtgat attgttgtg ccattcgtc 
      ttcagggaa aaatgagag tgctagaaa tttttgtag 
      cgtttatat tttttataa attcgagtt tatgtcaag 
      acaatgccg gtacaaatc tcgccccgg cgttgcaac 
      agtgttaca ataccaccc agtttgcca taagggact 
      acagattac catcagaca tgaccgtcc taattgaca 
      tcattcgaa gaatcgaag atggaaacg cattccagg 
      acgcccgag aactttctt tcgaaattc tccacaggt 
      ttcggcagg acgggatcc ggaggacca cgaaggacc 
      tggacagca cccagggca tccccttca gccccaagg 
      ggaaggagt ctttcaaga acagggtcc agttgaaga 
      agataacgc ccggaaacg cgtacaatt tcaataacg 
      acagcttaa cgccgctgc aagcctgtt cgctccatt 
      ttatgattt cgagtacga ttgatttgc acgaattag 
      ccgacgata ccgtgtgta acaaaatcg aacattaca 
      catcccggg tggaaaaac gagaaactg agtttgtgg 
      aagccgacc aaccgaaaa atcggtgcc ccgttgaaa 
      gcggcgttg tttacacga aatggggcg aagtcaaag 
      ctgggattt tgtttgccg ggattgcgg ccggttttc 
      agccaaatt ctgtgcctg tagtcaatg gtagcgtcg 
      actcggcct atgctagca cacttgcag gggaactgt 
      cggaggttg ctttgtacg aagtaggga aaccacgag 
      ttgtcggaa ataagggta gctggaaca cgaccattg 
      accggaaat actactttt tggcaaatt cggataagg 
      accacttgt atctgactg atgccgaga ttcgtggga 
      gcctcgatg gcttgtttc tggctctta acatccaaa 
      agtggaagt cacagtcga gtgacatca aaatttcac 
      aaattttag atctttatt acgggagag gggttttta 
      acgatacgg cacgagtgt gcaaccgga ataagttaa 
      tgcgagatt tagtagcaa gtgacaaac ttgtacaac 
      aaacttcct tttttaatt acttgctag atcaagaat 
      cgcagtacg attacacac ccatcaagc aaaacttcc 
      ccacgtttg ctcagtacg ctgtcgaga gcttgagat 
      cttatttag ataccaatc tgaaggatc gagagtgtt 
      cggcggaag aagatgtaa ttgagcgag tgtcctcac 
      atttattaa tttttgtcg atacgacct ggacttatc 
      aaacgattc agccgatta tttcccaca ttttgaaaa 
      acatcgatg tcaataaat acgaaactt cttatacaa 
      tttacgacg gtaccaact gtaacacaa ttgtcaata 
      gatcgagca gtattttgg acttttatg accagtata 
      acgtcaccc aacaccaaa aatatccca ccgggtttg 
      actatagca aagctatac aaacggcgg aagtagctt 
      ttaaggcga aattagaca acgccaccg atttgaccg 
      gacagtcga tgattgtcc taatgctgc cacataaca 
      aaccaactt caagccaaa gcagttatg ttcaaagtg 
      aaaaagagg atttcaaaa gcgttttgc ccgacgtta 
      gaattattg ttgctggat cagaaagta aagagtcgt 
      attccgaca tcgccacaa actccaaca cagacgttt 
      tcacgattt ctgaaggta gcgacgaga cattgcgac 
      atgcagccg aaaatatcg ttgccagat tgaaaaaaa 
      taccacgaa gtgttgtca accctaaat gtggttcct 
      ctttcgaaa ctgacgaaa ccagcagtt ttgaaataa 
      cacaatacg ttcaaccca ccacagtta attatcaca 
      aaatttcgc cgaattact tcaaggatg gacaaacgc 
      tgaagattc ggggacaag gtgctggca ctatcgctg 
      tgtgtatat cgagaactg atgccaatc cgagggcaa 
      atgccactg gggccaatt gtacaaaca tcgaatcca 
      acgagttga ctagagaca tcagtactt tttgcaccg 
      atgagtccc taaccgatt gctctccga tttatattt 
      ctgtccaag gaatgacat cgaacgttc gttgcacag 
      acaaatctt gccgattcc ccaacaaca tcaaataca 
      caatatctc tagaaaatg tcaagtgtt caagctgcg 
      gcaaactgc ttataaata atttgatga ttttcgtca 
      tgcttgttg tttactgct ttacttcta agtacctaa 
      attagtgat tacatattt ttaacaaaa tgtacatga 
      cctgacata aataggtgg tcacaagta gtataacca 
      aaactaatt aagctttaa atgccacgt cagtttctt 
      tatatatta gaagtacat ctgtaactg aatttttga 
      atatgcatt tataataaa ataaaaatt tagaaactt 
      aagtagttc ttgactatt gcaatcggc aaaaaaccc 
      gaactccat cgccggaaa tgcaaaatg agcaaaact 
      tttggaaac caggcattt gatcgagat aacatttca 
      gttttaagt aaaaactaa aagagcatt tgcataact 
      gggaaactg aagagggac aaaaaacga atgttcatc 
      tggcagaac gcacgcacc tcggatgaa aatgaagga 
      agcagattt ccgcaaaac tgtccgata atttttatt 
      gat