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Gene information
General information
TC010807 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5900755..5909234
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010807
    iBeetle screen iB_01737

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 6


    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 1
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010807 RA
      MTTKVDKPT SPTKNHAKN TSPKQDSGC GYKEECFEF 
      EPPPEAPVF HPTEEEFND PLEYINKIR KYAEGSGIC 
      KIKPPPNWQ PPFAVDVDK LRFTPRIQR LNELEAKTR 
      VKLNFLDQI AKFWELQGS TLKIPMVEK RCIDLYTLH 
      SIVQSQGGF DQVTKDRKW SKISVSMGY PSGKSIGTI 
      LKTHYERLL YPFDLFKQG KTLNFKMTS PLNEESEKA 
      DKDYKPHGI VGRMAIKPP PEKHARRSK RFDADPRVE 
      DDVKEDCKD DNKELKRLQ FYGAGPKIA ISKDKDDDK 
      RNNKTVNYD FDPLAKYVC HNCNRGDSE EYMLLCDGC 
      DDSYHTFCL MPPLNEIPK GDWRCPKCV AEEVSKPME 
      AFGFEQAQR EYTLQQFGD MADQFKSEY FNMPVHMVP 
      TNVVEKEFW RIVSSIDED VTVEYGADL HTMDHGSGF 
      PTKTSLNLL PGDKEYADS GWNLNNLPV LENSVLGYI 
      NADISGMKV PWMYVGMCF ATFCWHNED HWSYSINYL 
      HWGEAKTWY GVPGKMAEA FEETMKSAA PELFQSQPD 
      LLHQLVTIM NPNILMKAG VPVFRTDQH AGEFVVTFP 
      RAYHAGFNQ GYNFAEAVN FAPADWLRM GRECILHYS 
      NLRRFCVFS HDELVCKMA LDPDKLGLT IAAATYQDM 
      LQMVETEKT LRKTLLDAG VSNAEREAF ELLPDDERQ 
      CDHCKTTCF LSAVTCKCS PDILVCLRH YKNLCNCNP 
      ENYTLRYRY TLDELPVML KSLKLKAES FDHWVNRVK 
      DALDPKTPK TLTLQELKA LLNEADGKK FPKCDLLQT 
      LTDAVEDAE KCARVIQQL DLNKMRTRT RNSSDTKYK 
      LTLKELILF HEELDSLVC VLEEAKSIK DLLEETKKF 
      ETTSKALLE MKLSECSLS DLEQCISQG NNLCIELPN 
      LRSVKMRLR QCVWLNDVL SYKKKTEVL GLESIKNII 
      KTGTQLPPN PEIEVELSE LQALLSKSE EWEEKAQAI 
      LKDTGSEVL IEVDKLLKE AGQINSYLP SEEFLFEAM 
      DKSRDWLRQ LEEMNSAEC YPYFNDMEE LIKRGHQLA 
      LHLVEVSKL ESFLETANA WKEKTSRAF LRKNSSCTL 
      MEALSPRIQ FNSNSKCRK KNQEDEHGI FNLTSDMDP 
      ATVVALFKD AEQNEMELI RAVRAANLE KSMDPNDCA 
      SFCVCGRSL FGLMMQCEL CKDWFHSNC VPLPKVASS 
      KYKGNFTSV ALHLGFKDC KFLCPNCYR TKRPRLDAI 
      LLLLMSLQK LFVRVPEGE ALQSLTERA MNWQDRARQ 
      LIQQPELEA AKNKLASFS QKYSEAAAR QRTEKIISS 
      ELKRAYKNP DLLQRVQEI APFSGVTPD DKSDDTNIT 
      DSSDDSKGY GSADDRMGE HAYSLHFPK FDSGEYKLQ 
      IASDIRKQI EELLMEGDL LEVYLDETF QLWKLLQAS 
      RDPEKEAIL IDFDMNSKT APSKRGRKR RSDEVEMMK 
      KSKLVKDEN KKSKPKTKE TQGKKEGTR RKGRRRQST 
      GSEDEDEDS CAAPGCLKP SGENVDWVQ CDGGCDKWF 
      HMACVGLSA QDICEDEDY ICMTCSQNA YGTLNAVDS 
      FSSISSDCS TLSQPSTSK DSVQCKF
    CDS sequence
    • >TC010807 RA
      atgactaca aaagtggat aaacccacc agtcctact 
      aaaaaccac gcgaaaaac accagcccg aagcaagac 
      tcgggctgt ggctacaaa gaagagtgt ttcgagttc 
      gagcctcca ccggaggct cccgttttc cacccaacc 
      gaggaggaa ttcaacgat ccgctcgag tacatcaac 
      aagatcagg aagtatgct gagggatcg ggcatttgc 
      aagatcaaa ccgcctcca aattggcaa ccgcccttt 
      gccgttgac gttgacaaa cttagattt acgccacga 
      attcagaga cttaacgaa ttagaggct aaaactcgc 
      gtcaaatta aacttcctc gaccaaatc gccaaattc 
      tgggaactg caaggttcc acactgaaa atcccaatg 
      gtagaaaaa cgctgtatt gacctgtac acgttgcac 
      agcattgtt caaagtcaa ggtggtttt gaccaagta 
      accaaagac agaaagtgg tcgaaaatt tcggtctct 
      atggggtac ccatctggt aagagcata gggaccatt 
      ctaaaaacg cactatgag aggttgctt tacccgttt 
      gatcttttc aaacaaggc aaaactttg aattttaaa 
      atgacatca cccttaaat gaggaaagc gagaaggct 
      gataaagac tataaaccg cacgggatt gtggggcga 
      atggcgatc aaaccacca cctgagaaa cacgctcgc 
      cgatctaaa cgatttgat gccgacccg agagtcgaa 
      gacgatgtc aaagaagat tgcaaagac gacaataaa 
      gaactgaaa cgtttgcaa ttctacggc gccggccca 
      aaaatagcc attagcaag gacaaagac gatgacaaa 
      agaaacaac aaaactgtc aactacgac tttgatcct 
      ttggcaaag tacgtctgc cacaattgc aaccgcggc 
      gattctgaa gagtacatg ctgctgtgt gacggctgc 
      gacgacagc taccacacc ttttgcctg atgccaccc 
      ttgaacgag atcccgaaa ggggactgg cggtgcccg 
      aaatgtgtg gccgaggag gtgtccaag ccaatggaa 
      gccttcggc ttcgagcaa gctcaaaga gagtacact 
      ttgcagcaa ttcggcgat atggccgac cagtttaaa 
      tccgagtat ttcaacatg ccggttcac atggtgccc 
      acaaacgtc gtggagaag gaattctgg cgcattgtc 
      tcgtcaatt gacgaagac gtgacggtc gagtacggc 
      gccgatctg cacactatg gaccacggc tcgggcttt 
      cccaccaaa acgtccctg aacctcctg cctggggat 
      aaggagtac gccgattcc ggctggaat ttgaacaac 
      ctcccggtt ttggagaac tcggttttg gggtacatt 
      aacgcggat atttccggg atgaaggtc ccgtggatg 
      tacgtcggg atgtgtttc gcgacgttc tgttggcac 
      aacgaggac cattggagc tactccatc aattacctc 
      cattggggc gaagccaaa acttggtac ggagttcct 
      gggaaaatg gccgaggcc ttcgaagag acgatgaaa 
      tcggccgct cccgagctg ttccagtca cagcccgac 
      ttgctgcac cagttggtc acaatcatg aaccctaat 
      attttgatg aaggctgga gttcctgtg tttcggacg 
      gatcaacac gcgggggaa tttgtcgtt actttcccg 
      cgggcgtat catgccggg tttaatcag gggtataat 
      tttgccgag gctgtcaat ttcgcacca gctgattgg 
      ctgaggatg ggccgggag tgcatcttg cactattcg 
      aacttgcgc cgtttttgc gttttctcc cacgatgag 
      ttagtgtgt aaaatggct ctggacccg gataagtta 
      ggtctgacg attgccgca gctacatat caagacatg 
      ctacaaatg gtcgaaacc gagaaaact ttgcgaaag 
      acgctgctt gatgcggga gtgtctaat gccgagcgc 
      gaggctttt gagttgctt cctgatgac gaaaggcaa 
      tgcgatcat tgcaaaacg acatgcttc ctatctgcg 
      gtaacatgc aagtgttca ccggacatt ctcgtttgt 
      cttcgtcat tacaaaaat ttgtgtaat tgcaacccg 
      gagaattac acgttacgg tatcgctac actttagat 
      gaattgccg gtgatgctc aagtcccta aaactgaag 
      gcggagtcg ttcgaccat tgggtcaat cgcgtgaag 
      gatgcttta gatccgaaa actcccaaa acactgacg 
      ttacaagag ttgaaagct ctactcaac gaagcagac 
      gggaagaaa ttcccgaag tgtgatttg cttcaaact 
      ctaaccgac gcggtcgaa gatgccgag aaatgtgca 
      agagttatc caacagcta gatctaaat aaaatgcga 
      acaagaact cgaaactca tctgacacg aaatacaaa 
      ctgacgctt aaagaactg attcttttc cacgaagaa 
      ttggatagt ctagtgtgt gttttagaa gaagctaaa 
      agtattaaa gacttacta gaagaaacg aagaagttc 
      gaaacaact agcaaggct ttactcgaa atgaaactg 
      agtgaatgt tcactctcc gacttagag caatgtatt 
      agtcaagga aataatctg tgtatagaa cttccgaat 
      ttacgtagt gttaaaatg agattacgt cagtgtgtt 
      tggctgaac gacgtactg tcctacaag aagaaaacc 
      gaagttctt ggacttgag tcaattaaa aatattatt 
      aaaaccggt actcagcta ccaccaaat cctgaaatt 
      gaagtcgaa ttgagcgaa ctacaagcc ctcctgtcc 
      aaaagcgaa gaatgggag gaaaaagca caagcaatt 
      ctcaaagat accggaagc gaagtactt atcgaagtc 
      gataaattg ctaaaagaa gctggacaa atcaattcg 
      tatcttcca tctgaagag ttccttttt gaagccatg 
      gacaagtct cgagactgg ttgcgtcag ctagaagaa 
      atgaattcg gctgagtgt tacccctat ttcaacgac 
      atggaggag ttgattaaa aggggacac cagttggcg 
      ctccatttg gttgaagtc agcaaattg gagtcgttt 
      ttggagacc gcgaacgca tggaaggag aaaacaagt 
      cgggcgttc cttcgcaaa aactcgtcc tgtacccta 
      atggaagcc ctgtcgcct cgaatacaa ttcaacagc 
      aattcgaaa tgccgcaag aagaatcaa gaggacgag 
      catggcatt ttcaacctg accagtgac atggaccct 
      gctaccgtt gtagcactt ttcaaggat gccgaacaa 
      aatgaaatg gagttgata cgagcagtg cgcgctgca 
      aacttggag aaaagcatg gacccgaac gattgtgcc 
      tcgttttgc gtctgcggg cgcagcctt ttcggcttg 
      atgatgcag tgtgagctg tgcaaagat tggttccat 
      tcaaactgc gtccctctt cccaaagtc gcgtcttca 
      aagtacaaa ggcaacttt acgagtgtt gcactacat 
      ttgggattc aaagactgt aaattcctc tgtcctaac 
      tgctaccgg accaagcgt ccacgtcta gacgcgatc 
      ctcctgctc ttgatgtcc ttgcagaag ctctttgtc 
      agggtgccg gaaggcgag gctttgcag agtttgact 
      gaacgcgct atgaactgg caagaccgt gcccgccaa 
      ctcatccaa caaccggaa ctggaagca gctaaaaac 
      aaactcgct tcgttcagt cagaagtat tcggaagca 
      gcagcgagg caaagaact gagaagatc atttcgagc 
      gagctaaag cgcgcatat aagaacccc gatttgtta 
      caaagagtg caagagata gcacccttc agtggagtc 
      accccggat gataagtcg gatgatacg aacatcacc 
      gattcgtca gacgactcc aagggttac ggttcggcg 
      gatgaccga atgggagaa cacgcttac tccctccat 
      ttcccgaaa tttgactcg ggagaatat aagttgcaa 
      atcgcttca gatatcagg aagcagatt gaggagttg 
      ttgatggaa ggggatttg cttgaggtt tatctcgat 
      gaaactttc caactctgg aagttgctc caagcgtcg 
      cgggatccg gagaaagag gctattttg atcgatttt 
      gatatgaat tcgaagact gcgccgtcg aagcgcggc 
      cgcaagcgt cgttcggac gaggtcgaa atgatgaag 
      aagtccaaa ctggtgaaa gacgaaaac aagaagtcc 
      aaaccgaaa accaaagaa acgcaaggg aagaaagag 
      ggcacaaga cgcaagggc cgccggcgc cagtccacc 
      ggctcggag gacgaggac gaggacagt tgcgctgca 
      ccgggctgt ctgaagccg tcgggagag aacgtcgac 
      tgggttcag tgcgacggt gggtgcgat aagtggttt 
      cacatggcc tgtgtcggg ttgtcagcg caggatatt 
      tgcgaagat gaggattac atttgtatg acctgctca 
      caaaatgct tacggaaca ctcaatgcc gtcgatagt 
      ttctcgtct atcagttct gattgttcc actttatcc 
      cagccttcc acttcgaaa gactctgtt cagtgcaaa 
      ttttag
    mRNA sequence
    • >TC010807 RA
      tatgcgtca cgtaccttt ctagttaac ctaaaagtt 
      ggtatttat ggtgcaaaa tgttggacg gaggtgttt 
      gaggctgaa ctaggagca aagtgcgtg gtgacttaa 
      ttatgtaaa attacccaa aatgactac aaaagtgga 
      taaacccac cagtcctac taaaaacca cgcgaaaaa 
      caccagccc gaagcaaga ctcgggctg tggctacaa 
      agaagagtg tttcgagtt cgagcctcc accggaggc 
      tcccgtttt ccacccaac cgaggagga attcaacga 
      tccgctcga gtacatcaa caagatcag gaagtatgc 
      tgagggatc gggcatttg caagatcaa accgcctcc 
      aaattggca accgccctt tgccgttga cgttgacaa 
      acttagatt tacgccacg aattcagag acttaacga 
      attagaggc taaaactcg cgtcaaatt aaacttcct 
      cgaccaaat cgccaaatt ctgggaact gcaaggttc 
      cacactgaa aatcccaat ggtagaaaa acgctgtat 
      tgacctgta cacgttgca cagcattgt tcaaagtca 
      aggtggttt tgaccaagt aaccaaaga cagaaagtg 
      gtcgaaaat ttcggtctc tatggggta cccatctgg 
      taagagcat agggaccat tctaaaaac gcactatga 
      gaggttgct ttacccgtt tgatctttt caaacaagg 
      caaaacttt gaattttaa aatgacatc acccttaaa 
      tgaggaaag cgagaaggc tgataaaga ctataaacc 
      gcacgggat tgtggggcg aatggcgat caaaccacc 
      acctgagaa acacgctcg ccgatctaa acgatttga 
      tgccgaccc gagagtcga agacgatgt caaagaaga 
      ttgcaaaga cgacaataa agaactgaa acgtttgca 
      attctacgg cgccggccc aaaaatagc cattagcaa 
      ggacaaaga cgatgacaa aagaaacaa caaaactgt 
      caactacga ctttgatcc tttggcaaa gtacgtctg 
      ccacaattg caaccgcgg cgattctga agagtacat 
      gctgctgtg tgacggctg cgacgacag ctaccacac 
      cttttgcct gatgccacc cttgaacga gatcccgaa 
      aggggactg gcggtgccc gaaatgtgt ggccgagga 
      ggtgtccaa gccaatgga agccttcgg cttcgagca 
      agctcaaag agagtacac tttgcagca attcggcga 
      tatggccga ccagtttaa atccgagta tttcaacat 
      gccggttca catggtgcc cacaaacgt cgtggagaa 
      ggaattctg gcgcattgt ctcgtcaat tgacgaaga 
      cgtgacggt cgagtacgg cgccgatct gcacactat 
      ggaccacgg ctcgggctt tcccaccaa aacgtccct 
      gaacctcct gcctgggga taaggagta cgccgattc 
      cggctggaa tttgaacaa cctcccggt tttggagaa 
      ctcggtttt ggggtacat taacgcgga tatttccgg 
      gatgaaggt cccgtggat gtacgtcgg gatgtgttt 
      cgcgacgtt ctgttggca caacgagga ccattggag 
      ctactccat caattacct ccattgggg cgaagccaa 
      aacttggta cggagttcc tgggaaaat ggccgaggc 
      cttcgaaga gacgatgaa atcggccgc tcccgagct 
      gttccagtc acagcccga cttgctgca ccagttggt 
      cacaatcat gaaccctaa tattttgat gaaggctgg 
      agttcctgt gtttcggac ggatcaaca cgcggggga 
      atttgtcgt tactttccc gcgggcgta tcatgccgg 
      gtttaatca ggggtataa ttttgccga ggctgtcaa 
      tttcgcacc agctgattg gctgaggat gggccggga 
      gtgcatctt gcactattc gaacttgcg ccgtttttg 
      cgttttctc ccacgatga gttagtgtg taaaatggc 
      tctggaccc ggataagtt aggtctgac gattgccgc 
      agctacata tcaagacat gctacaaat ggtcgaaac 
      cgagaaaac tttgcgaaa gacgctgct tgatgcggg 
      agtgtctaa tgccgagcg cgaggcttt tgagttgct 
      tcctgatga cgaaaggca atgcgatca ttgcaaaac 
      gacatgctt cctatctgc ggtaacatg caagtgttc 
      accggacat tctcgtttg tcttcgtca ttacaaaaa 
      tttgtgtaa ttgcaaccc ggagaatta cacgttacg 
      gtatcgcta cactttaga tgaattgcc ggtgatgct 
      caagtccct aaaactgaa ggcggagtc gttcgacca 
      ttgggtcaa tcgcgtgaa ggatgcttt agatccgaa 
      aactcccaa aacactgac gttacaaga gttgaaagc 
      tctactcaa cgaagcaga cgggaagaa attcccgaa 
      gtgtgattt gcttcaaac tctaaccga cgcggtcga 
      agatgccga gaaatgtgc aagagttat ccaacagct 
      agatctaaa taaaatgcg aacaagaac tcgaaactc 
      atctgacac gaaatacaa actgacgct taaagaact 
      gattctttt ccacgaaga attggatag tctagtgtg 
      tgttttaga agaagctaa aagtattaa agacttact 
      agaagaaac gaagaagtt cgaaacaac tagcaaggc 
      tttactcga aatgaaact gagtgaatg ttcactctc 
      cgacttaga gcaatgtat tagtcaagg aaataatct 
      gtgtataga acttccgaa tttacgtag tgttaaaat 
      gagattacg tcagtgtgt ttggctgaa cgacgtact 
      gtcctacaa gaagaaaac cgaagttct tggacttga 
      gtcaattaa aaatattat taaaaccgg tactcagct 
      accaccaaa tcctgaaat tgaagtcga attgagcga 
      actacaagc cctcctgtc caaaagcga agaatggga 
      ggaaaaagc acaagcaat tctcaaaga taccggaag 
      cgaagtact tatcgaagt cgataaatt gctaaaaga 
      agctggaca aatcaattc gtatcttcc atctgaaga 
      gttcctttt tgaagccat ggacaagtc tcgagactg 
      gttgcgtca gctagaaga aatgaattc ggctgagtg 
      ttaccccta tttcaacga catggagga gttgattaa 
      aaggggaca ccagttggc gctccattt ggttgaagt 
      cagcaaatt ggagtcgtt tttggagac cgcgaacgc 
      atggaagga gaaaacaag tcgggcgtt ccttcgcaa 
      aaactcgtc ctgtaccct aatggaagc cctgtcgcc 
      tcgaataca attcaacag caattcgaa atgccgcaa 
      gaagaatca agaggacga gcatggcat tttcaacct 
      gaccagtga catggaccc tgctaccgt tgtagcact 
      tttcaagga tgccgaaca aaatgaaat ggagttgat 
      acgagcagt gcgcgctgc aaacttgga gaaaagcat 
      ggacccgaa cgattgtgc ctcgttttg cgtctgcgg 
      gcgcagcct tttcggctt gatgatgca gtgtgagct 
      gtgcaaaga ttggttcca ttcaaactg cgtccctct 
      tcccaaagt cgcgtcttc aaagtacaa aggcaactt 
      tacgagtgt tgcactaca tttgggatt caaagactg 
      taaattcct ctgtcctaa ctgctaccg gaccaagcg 
      tccacgtct agacgcgat cctcctgct cttgatgtc 
      cttgcagaa gctctttgt cagggtgcc ggaaggcga 
      ggctttgca gagtttgac tgaacgcgc tatgaactg 
      gcaagaccg tgcccgcca actcatcca acaaccgga 
      actggaagc agctaaaaa caaactcgc ttcgttcag 
      tcagaagta ttcggaagc agcagcgag gcaaagaac 
      tgagaagat catttcgag cgagctaaa gcgcgcata 
      taagaaccc cgatttgtt acaaagagt gcaagagat 
      agcaccctt cagtggagt caccccgga tgataagtc 
      ggatgatac gaacatcac cgattcgtc agacgactc 
      caagggtta cggttcggc ggatgaccg aatgggaga 
      acacgctta ctccctcca tttcccgaa atttgactc 
      gggagaata taagttgca aatcgcttc agatatcag 
      gaagcagat tgaggagtt gttgatgga aggggattt 
      gcttgaggt ttatctcga tgaaacttt ccaactctg 
      gaagttgct ccaagcgtc gcgggatcc ggagaaaga 
      ggctatttt gatcgattt tgatatgaa ttcgaagac 
      tgcgccgtc gaagcgcgg ccgcaagcg tcgttcgga 
      cgaggtcga aatgatgaa gaagtccaa actggtgaa 
      agacgaaaa caagaagtc caaaccgaa aaccaaaga 
      aacgcaagg gaagaaaga gggcacaag acgcaaggg 
      ccgccggcg ccagtccac cggctcgga ggacgagga 
      cgaggacag ttgcgctgc accgggctg tctgaagcc 
      gtcgggaga gaacgtcga ctgggttca gtgcgacgg 
      tgggtgcga taagtggtt tcacatggc ctgtgtcgg 
      gttgtcagc gcaggatat ttgcgaaga tgaggatta 
      catttgtat gacctgctc acaaaatgc ttacggaac 
      actcaatgc cgtcgatag tttctcgtc tatcagttc 
      tgattgttc cactttatc ccagccttc cacttcgaa 
      agactctgt tcagtgcaa attttagtc ataattgtc 
      ccgcgagga ctgatacaa gtattttag tatagataa 
      tgtatacaa tgttcagtt gtgataatt tacgataag 
      aaatgtaaa aatattttc atgtatcat tttcatttt 
      tacaccttt tattatact ttctttatg gtttcgttg 
      ttaagtcta cgtagttaa tgtcctgta tttacacgt 
      taatttacc cacagttca taatgttcg tttttcaca 
      tttttttta taaatgaac tgcggctaa attatttac 
      aagaaactt tatttattt ttattctga ttatgcacc 
      gagtctgcg ctttagaga ttatgtata tttttataa 
      tttttcttt tatcataaa ctgagtagg tactaagta 
      ttaatattt aactttatt gcccttgca atttttttt 
      gtcaataaa tacttttgg atact
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