Status
Please wait ...
Gene information
General information
TC010804 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5874345..5880292
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010804
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01736

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    appendages partially remaining - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    antenna mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    metathorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    mesothorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    prothoracic leg shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    prothoracic leg pretarsus not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010804 RA
      MSTFISKPL GGWIHSDKL ISKQGATYA VRYVGCLEV 
      KVSMKILNF KTRSTVAKE CINKVCEAA GLKTADKKR 
      KVDKKVLKA IAEVPNMTH AGANVSLNV SSSNLSLTS 
      LDDNKVIAC HDMPHISFA SGGDIDTMD FVGYIAKDI 
      NDFRACYVL ECGGGLAKD VIATIGQAF ELRFKQYMK 
      PAQIGLAMG QPSTSTGGD ADKDYYNDL PGKVPPDVG 
      PPPVPPLPS VVPPFTTLP SVQSPNLID LNSDISVFD 
      TPHHDYVND SITIKERDV FDMQPFTTH HQPPIRSIE 
      EHLAELQHE IWFHGQISR ADAERLLQK DGDFLVRES 
      PNSEGQFVL SGIQDDTKK HLLLIDPEG VIRTRDRMF 
      NSVSHLINF HCENVLPII SAESVLVLR NPVPRIS
    CDS sequence
    • >TC010804 RA
      atgtcgacg tttattagc aagccactg ggtgggtgg 
      attcactcc gataaacta atttcgaag caaggggcg 
      acttatgca gttaggtat gttggatgt ctggaggtg 
      aaagtctcc atgaaaatc ctcaatttc aaaacccgg 
      tcgactgtt gccaaagaa tgtataaat aaggtctgt 
      gaggccgcc gggttaaaa acggccgat aagaaacga 
      aaagtcgac aagaaagtc ttaaaagcc attgctgaa 
      gttcctaat atgacccac gcaggggcg aacgttagt 
      ctaaatgta tcaagttcg aacctatct ttaacttcg 
      ctagatgac aataaagtc attgcgtgt catgacatg 
      ccccacatc tcgtttgct tcagggggc gatattgac 
      actatggac tttgttggt tacattgcg aaagacatc 
      aacgacttt agagcctgc tatgttcta gaatgtggg 
      ggtgggtta gccaaagat gttatagca accataggg 
      caagctttc gaactgagg ttcaaacaa tacatgaaa 
      ccagcacaa atcgggctt gcgatgggc cagccgtcg 
      acgtccaca ggtggcgac gccgataaa gactactac 
      aacgatctt ccaggcaaa gtacctccc gatgtgggt 
      cctccacct gtccctccg cttccttcc gtcgtgcca 
      ccatttaca accctacca tcagtccaa agtcctaac 
      ttaatagac cttaattcc gacatttcc gttttcgat 
      acgccacat cacgattac gttaatgat agtattacg 
      attaaagaa agggacgtt tttgatatg caaccgttt 
      acgacgcat caccaacca ccgattaga agtattgaa 
      gaacactta gcggaatta cagcatgaa atttggttt 
      catggacag atcagtcgc gcagatgct gagcgactt 
      ctccagaag gatggggat tttctcgtg cgggagtcg 
      ccgaattcg gaaggacaa ttcgtgctt tcggggatt 
      caagatgac acgaagaaa catctcctg ttgatagat 
      cctgaagga gtgattcgg acgagggat cggatgttc 
      aacagtgtt agccatctc atcaacttc cactgcgag 
      aacgtcctc cccattata tcggctgaa agcgttcta 
      gtacttcgc aacccagta ccaagaata tcatag
    mRNA sequence
    • >TC010804 RA
      tcggcgaag tgagccatg ttcatgacg taaacggtt 
      agaaaacaa aagcaaaat gaggcgggt gtgcaatgt 
      gagcctgca agcgtagca tgtcctccc caaagtcga 
      cttattttt gaatttctg ttgctatgt gcgcctcta 
      gaaatgcga aaatcccct cgtttgcat cgaaataat 
      gaaaaaatt gtgatacta gtttagtaa gttaaatgt 
      gaggaatta gtgcgtact gcgtagttg gccgtggaa 
      accgatgct tctcgacgt agacgtcat attgggtga 
      attggcaag ttttcttgt gaaaaatcg tcgattttc 
      ctcgctgca gcgagaaaa accggtgga aaagaacta 
      gtcaggcag gttcacacc catcttatc gcaggaatt 
      cgcagcgaa aatgtcgac gtttattag caagccact 
      gggtgggtg gattcactc cgataaact aatttcgaa 
      gcaaggggc gacttatgc agttaggta tgttggatg 
      tctggaggt gaaagtctc catgaaaat cctcaattt 
      caaaacccg gtcgactgt tgccaaaga atgtataaa 
      taaggtctg tgaggccgc cgggttaaa aacggccga 
      taagaaacg aaaagtcga caagaaagt cttaaaagc 
      cattgctga agttcctaa tatgaccca cgcaggggc 
      gaacgttag tctaaatgt atcaagttc gaacctatc 
      tttaacttc gctagatga caataaagt cattgcgtg 
      tcatgacat gccccacat ctcgtttgc ttcaggggg 
      cgatattga cactatgga ctttgttgg ttacattgc 
      gaaagacat caacgactt tagagcctg ctatgttct 
      agaatgtgg gggtgggtt agccaaaga tgttatagc 
      aaccatagg gcaagcttt cgaactgag gttcaaaca 
      atacatgaa accagcaca aatcgggct tgcgatggg 
      ccagccgtc gacgtccac aggtggcga cgccgataa 
      agactacta caacgatct tccaggcaa agtacctcc 
      cgatgtggg tcctccacc tgtccctcc gcttccttc 
      cgtcgtgcc accatttac aaccctacc atcagtcca 
      aagtcctaa cttaataga ccttaattc cgacatttc 
      cgttttcga tacgccaca tcacgatta cgttaatga 
      tagtattac gattaaaga aagggacgt ttttgatat 
      gcaaccgtt tacgacgca tcaccaacc accgattag 
      aagtattga agaacactt agcggaatt acagcatga 
      aatttggtt tcatggaca gatcagtcg cgcagatgc 
      tgagcgact tctccagaa ggatgggga ttttctcgt 
      gcgggagtc gccgaattc ggaaggaca attcgtgct 
      ttcggggat tcaagatga cacgaagaa acatctcct 
      gttgataga tcctgaagg agtgattcg gacgaggga 
      tcggatgtt caacagtgt tagccatct catcaactt 
      ccactgcga gaacgtcct ccccattat atcggctga 
      aagcgttct agtacttcg caacccagt accaagaat 
      atcatagtt tttagttgg tgaatatta atctggctt 
      gcactagct tctatgttc tagtactgt tcattgcaa 
      gtttagcaa attatagta caaagattt tcatgccat 
      atagtaaaa attcgaaac ggtttttgc taaacttgt 
      ttagtgaga tcttttctt ttattccgg gcgaacggt 
      tatgttttc catatggca cagcataat catatcaaa 
      agtattcaa ggaacttta aacaagtat taatttttt 
      acgcattta taacgtgtt atggtaaca gtcgaagcc 
      tcatgctta atcaaacat aacttgatg tgatttagg 
      tagttttaa ttgtgcata tatgattgc gacaatttg 
      tgatagatc ccaggtttg gacaacatg aagggtgga 
      ttaaatttt acaaacacg atttacgtt tttatttat 
      tatttatta tgatttgga taacacatt acataaaat 
      tattttcgg ttttccggc tgagtttcc atttccttt 
      cgtgtcttc ttacgcagg acctaaaca aaaactaca 
      ctttagtaa caacataaa taaaaaata attgaagta 
      gagagaaca tggcggcgt ctaaaagta aggttacgg 
      gtatgcgca acaaaagtg acaattatc ttatttcac 
      taattcaaa cttggttca tctgaaaac taaccatgt 
      agttataaa atagctgaa tgcaccgtt atcctgacc 
      tgactgttt acgctattt cgtccgttt tgttttatt 
      aattggtgg tatactttt gcaacataa tgcccttca 
      agttttcta acttgggat attttaaca gaaattgaa 
      gaatgtttt aaaatgttc gaaaacaac tattttttt 
      actaactgg accagtaaa gttgttaat ctatgacta 
      aaaagacac ttccaacgc tctctagat acagaaacg 
      tacttgaat ttgcaaatg tcttgaacg ttttagaag 
      attttcaac gtttgatat atcttttgg acgttttta 
      tacttatgc tgtgttgca ttatttttg atgcaaagt 
      tttgatttt tactgcttt tgaatgcaa gactgcggc 
      atagtcgtg tttccactt ttggaaaat gcgttcatg 
      tggacatga ctgtgcacc aaaacgtta gaatagaga 
      cgcaatttg cggaatagt ctacaagtg accttaaat 
      cgcacagaa accgattga atttggcgc gctcgagac 
      gaaccaaaa gtctgtgca acattcctg aattgatta 
      ggtttttgt gcaattagt acaggtgct acgtgtcga 
      tttttaggt tagaattgg atattttct ttaaaattt 
      caacattag ttccgtctg gaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY