| General information | |||
| TC010798 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 5689166..5695403 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC010798 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01735 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)beetle stock: bad (increased lethality), adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 5 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
- >TC010798 RA
MPAVRGDGM RGLAVFISD IRNCKSKEA EIKRINKEL ANIRSKFKG DKTLDGYQK KKYVCKLLF IFLLGHDID FGHMEAVNL LSSNKYSEK QIGYLFISV LVNTNSELI KLIIQSIKN DLASRNPIH VNLALQCIA NIGSREMAE AFGNEIPKL LVSGDTMDV VKQSAALCL LRLFRTSQE IIPGGEWTS RIIHLLNDQ HMGVVTAAT SLIDALVKK NPEEYKGCV SLAVSRLSR IVTASYTDL QDYTYYFVP APWLSVKLL RLLQNYTPP AEDPGVRGR LNECLETIL NKAQEPPKS KKVQHSNAK NAVLFEAIS LIIHNDSEA NLLVRACNQ LGQFLSNRE TNLRYLALE SMCHLATSE FSHEAVKKH QEVVILSMK MEKDVSVRQ QAVDLLYAM CDKSNAEEI VQEMLNYLE TADYSIREE MVLKVAILA EKYATDYTW YVDVILNLI RIAGDYVSE EVWYRVIQI VINRDEVQG YAAKTVFEA LQAPACHEN MVKVGGYIL GEFGNLIAG DQRSSPSVQ FQLLHSKYH LCSPMTRAL LLSTYIKFI NLFPEIRTQ VQEVFKQHS NLRSADAEL QQRASEYLQ LSIIASSDV LATVLEEMP AFPERESSI LAVLKKKKP GRVPENEIK ENKSPTPVT NHNNDVNNS SAVSGDLLG LSTPPTAQP NSGNTGVLL DVLGDIYGG KQANNSNSA TQPVNMYNP KKFVCKNNG VLFESDLIQ IGVKSEFRQ NLGRLGLFY GNKTNVPLQ NFVPTLSWP DDQASKLTI QMKPVEPVL EAGAQIQQM INAECVDDY TGAPSIVLS FLYLNVPQK ITIKLPLTI NKFFEPTEM NGESFFARW KNLGSTNQQ RSQKIFRAT QQMDLQAAR TKIMGFGMQ LLDGIDPNP DNFVCAGII HMRSQQVGC LLRLEPNKS AQMYRLTVR SSKESVSIE ICDLLADQF
- >TC010798 RA
atgccggca gtgcgagga gatggcatg cgtggcttg gccgtattt atttccgat ataagaaat tgtaaaagc aaagaagcc gagatcaag cgcattaac aaggagctc gcgaatata cggagcaag ttcaagggg gacaagact ctggatggg taccagaag aagaagtac gtgtgcaag ctgctcttc atattctta ctcgggcat gacattgac ttcgggcat atggaagct gtaaatctc ctatcatcc aacaaatac tccgaaaag caaatagga tatttattt atttcagtg ctagtcaac actaatagt gagctgatc aaactcata attcaaagt attaaaaat gacttggct tcgagaaac ccaattcac gtgaacttg gcattgcaa tgtattgcc aacatcggc agcagggaa atggccgaa gcctttggt aacgaaata ccaaaactt ctcgtctcg ggcgacacc atggatgta gttaagcaa agtgcagct ctctgtctt ctgcgtttg ttcagaacg tcgcaggaa atcatcccc ggaggggag tggacttca cgcatcata cacctcctc aatgaccaa cacatgggg gttgtgaca gccgcgaca agtctcata gacgcatta gttaagaag aaccctgag gagtacaaa ggctgcgtt tccctcgcc gtttcgcgc ctaagtaga atcgtgacg gcaagttac accgattta caagactac acatattat ttcgtacca gcgccctgg ctctccgtc aaactgcta cgtctcttg caaaattat accccgcca gctgaagac ccgggggtg cgcggccgc ttgaacgaa tgcctagaa acgatattg aataaagcg caggagcct ccaaaatcg aagaaagtg caacactca aatgctaaa aatgccgta ctgttcgag gccattagt ttaattatt cacaatgac agcgaagcg aatttgttg gtgcgtgct tgtaaccag ttgggccaa tttttgagt aatagagaa acaaattta cgttatttg gctttggag tcgatgtgt catttagca acgtctgaa ttttcccac gaggctgtt aagaaacat caggaagtt gttatcttg tcgatgaag atggaaaaa gatgtttca gtgaggcaa caagccgtt gatttgttg tacgctatg tgtgataag agcaatgct gaggaaatc gtccaagaa atgttgaat tatttggaa acggctgat tactcaatt agggaagaa atggtccta aaagttgcg attttggcc gagaaatac gccacggat tacacttgg tatgttgat gttatttta aatttgata agaatcgct ggcgattat gtgtcggaa gaggtgtgg tatcgagtg atccaaatc gttatcaac cgagatgaa gtgcaaggt tacgccgct aaaactgtc tttgaagct ttgcaagca cccgcctgt cacgagaac atggtcaaa gtcgggggt tacatcctg ggagagttt ggcaattta atcgccggg gatcagaga tcctcaccc tcagttcaa ttccaactt ctccactca aaataccac ttatgttcg ccgatgact cgagctttg ttactctcg acttacatc aaattcatc aatttgttc cccgaaata cggactcaa gtacaggaa gttttcaaa cagcacagc aacctgagg tcggctgac gccgaattg caacaacga gcttcggaa tatttgcaa ttgagtatt attgcaagt tcggacgtt ttggccaca gtattggag gaaatgccg gcgttcccc gaacgagag agctcgatt cttgctgtt ttgaagaag aaaaaacca ggacgggtc ccagaaaat gaaattaag gagaacaaa agtcccaca cctgtgacc aaccacaac aacgatgta aataacagc agtgctgtc tctggcgat ttgttgggt ctgtcgact ccgccaaca gcgcagccc aactccgga aacaccggg gtgctcctg gacgtcctt ggggacatc tacgggggc aaacaggcg aacaactcg aacagtgcc actcaaccg gtcaacatg tacaacccc aagaagttc gtgtgtaaa aacaacggc gttttattc gaaagtgac ttgatccag ataggcgtt aaaagcgag ttccgccag aatttgggc cgacttggc ttgttttac ggaaataag actaacgtt ccgttgcaa aatttcgtc ccgaccttg tcttggccc gacgatcag gcttccaag ctcaccatt cagatgaag cctgttgag ccggtcctg gaagctggt gctcaaatc caacagatg atcaacgcg gagtgtgtc gatgactac acgggcgcc cccagcata gtgctcagt ttcctgtat ctcaacgtg ccccagaaa ataacgatc aaacttcca ttgacgatt aacaagttc tttgaaccg actgaaatg aacggcgaa tccttcttc gcaaggtgg aaaaacctc ggaagtacg aaccagcaa agatctcag aagatattt agggcgaca cagcagatg gacttgcaa gccgcgcga acaaaaata atgggattc gggatgcaa ctcctggac gggattgat ccaaatccg gataatttc gtctgtgct gggattatc cacatgcgg tcgcagcaa gtcggatgt cttcttcgt ttagagccg aacaagagt gcacaaatg tacagactg acggtgcgc tctagtaaa gaaagcgtc tcgatagaa atttgtgat cttctagcg gatcaattt taa
- >TC010798 RA
cgtcctgtc aatttgccc atgtatatg tggagtggc aaagattgg tgtgtaaaa agatatttt gttgacaat tgaccgtat ttatcgctt gtttgtgta gataaataa ttgtggaaa tcggatatt taaagaaaa gttaggagg attttggag gaacgaagc gatttcagc caacatgcc ggcagtgcg aggagatgg catgcgtgg cttggccgt atttatttc cgatataag aaattgtaa aagcaaaga agccgagat caagcgcat taacaagga gctcgcgaa tatacggag caagttcaa gggggacaa gactctgga tgggtacca gaagaagaa gtacgtgtg caagctgct cttcatatt cttactcgg gcatgacat tgacttcgg gcatatgga agctgtaaa tctcctatc atccaacaa atactccga aaagcaaat aggatattt atttatttc agtgctagt caacactaa tagtgagct gatcaaact cataattca aagtattaa aaatgactt ggcttcgag aaacccaat tcacgtgaa cttggcatt gcaatgtat tgccaacat cggcagcag ggaaatggc cgaagcctt tggtaacga aataccaaa acttctcgt ctcgggcga caccatgga tgtagttaa gcaaagtgc agctctctg tcttctgcg tttgttcag aacgtcgca ggaaatcat ccccggagg ggagtggac ttcacgcat catacacct cctcaatga ccaacacat gggggttgt gacagccgc gacaagtct catagacgc attagttaa gaagaaccc tgaggagta caaaggctg cgtttccct cgccgtttc gcgcctaag tagaatcgt gacggcaag ttacaccga tttacaaga ctacacata ttatttcgt accagcgcc ctggctctc cgtcaaact gctacgtct cttgcaaaa ttatacccc gccagctga agacccggg ggtgcgcgg ccgcttgaa cgaatgcct agaaacgat attgaataa agcgcagga gcctccaaa atcgaagaa agtgcaaca ctcaaatgc taaaaatgc cgtactgtt cgaggccat tagtttaat tattcacaa tgacagcga agcgaattt gttggtgcg tgcttgtaa ccagttggg ccaattttt gagtaatag agaaacaaa tttacgtta tttggcttt ggagtcgat gtgtcattt agcaacgtc tgaattttc ccacgaggc tgttaagaa acatcagga agttgttat cttgtcgat gaagatgga aaaagatgt ttcagtgag gcaacaagc cgttgattt gttgtacgc tatgtgtga taagagcaa tgctgagga aatcgtcca agaaatgtt gaattattt ggaaacggc tgattactc aattaggga agaaatggt cctaaaagt tgcgatttt ggccgagaa atacgccac ggattacac ttggtatgt tgatgttat tttaaattt gataagaat cgctggcga ttatgtgtc ggaagaggt gtggtatcg agtgatcca aatcgttat caaccgaga tgaagtgca aggttacgc cgctaaaac tgtctttga agctttgca agcacccgc ctgtcacga gaacatggt caaagtcgg gggttacat cctgggaga gtttggcaa tttaatcgc cggggatca gagatcctc accctcagt tcaattcca acttctcca ctcaaaata ccacttatg ttcgccgat gactcgagc tttgttact ctcgactta catcaaatt catcaattt gttccccga aatacggac tcaagtaca ggaagtttt caaacagca cagcaacct gaggtcggc tgacgccga attgcaaca acgagcttc ggaatattt gcaattgag tattattgc aagttcgga cgttttggc cacagtatt ggaggaaat gccggcgtt ccccgaacg agagagctc gattcttgc tgttttgaa gaagaaaaa accaggacg ggtcccaga aaatgaaat taaggagaa caaaagtcc cacacctgt gaccaacca caacaacga tgtaaataa cagcagtgc tgtctctgg cgatttgtt gggtctgtc gactccgcc aacagcgca gcccaactc cggaaacac cggggtgct cctggacgt ccttgggga catctacgg gggcaaaca ggcgaacaa ctcgaacag tgccactca accggtcaa catgtacaa ccccaagaa gttcgtgtg taaaaacaa cggcgtttt attcgaaag tgacttgat ccagatagg cgttaaaag cgagttccg ccagaattt gggccgact tggcttgtt ttacggaaa taagactaa cgttccgtt gcaaaattt cgtcccgac cttgtcttg gcccgacga tcaggcttc caagctcac cattcagat gaagcctgt tgagccggt cctggaagc tggtgctca aatccaaca gatgatcaa cgcggagtg tgtcgatga ctacacggg cgcccccag catagtgct cagtttcct gtatctcaa cgtgcccca gaaaataac gatcaaact tccattgac gattaacaa gttctttga accgactga aatgaacgg cgaatcctt cttcgcaag gtggaaaaa cctcggaag tacgaacca gcaaagatc tcagaagat atttagggc gacacagca gatggactt gcaagccgc gcgaacaaa aataatggg attcgggat gcaactcct ggacgggat tgatccaaa tccggataa tttcgtctg tgctgggat tatccacat gcggtcgca gcaagtcgg atgtcttct tcgtttaga gccgaacaa gagtgcaca aatgtacag actgacggt gcgctctag taaagaaag cgtctcgat agaaatttg tgatcttct agcggatca attttaatt aatttagaa aattgtgta agtaagagt gcattccat agtagactt accttattg tttaaaaat taatatatt gaaatttgc acagacatg cgctctttt ttcgtttga gattcgctt acagtaaaa tattagaaa gaggatgtt ctttgagac aatatgtaa gtgtgtgac gttagtgtt gcttgttga cagttaatt tatagagat atgaatttt ttgctgaat tcataacat tagacctct attcggaag aaaagggtc cattgcgga aaattaaat gtacaataa acgtttttt gttgagaca gatctgctg cactcgaat taaaccaaa aactaaggt gggtcaaac gatcttctt gaatgtata ttaaaaatt caatatgaa ataaaacta tatcattag agacgtgta acttgtgtt atattgtta tatcatacc agaggatgg tcgacgagt tggtaatga atcgttctt cagatgagg agtttattg aaacagttg actttctac tgtcctcag atatatttt ttaccattt attacaata actgattac gttgtgtag tatgccttg aattattat ttgtatatg ttgattgta taaataagt aaaatattg atttagtaa tacaaactc tttgcaatt ccatttttg tattcctat tttttatat catctacaa agtgcatga tactttatg taaaaataa tattgtgta atatatgaa ttcaaagct aaagttgtt gtaacatta tatgcgatt atttaacat ttttgtgtt tctgttgta ttgatgctt caattacaa aaaatcacc gtcaacatt tttcattac t
