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Gene information
General information
TC010798 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5689166..5695403
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010798
    iBeetle screen iB_01735

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    beetle stock: bad (increased lethality), adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection

    Pupal morphology
    beetle stock: bad (increased lethality),
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010798 RA
      MPAVRGDGM RGLAVFISD IRNCKSKEA EIKRINKEL 
ANIRSKFKG DKTLDGYQK KKYVCKLLF IFLLGHDID 
FGHMEAVNL LSSNKYSEK QIGYLFISV LVNTNSELI 
KLIIQSIKN DLASRNPIH VNLALQCIA NIGSREMAE 
AFGNEIPKL LVSGDTMDV VKQSAALCL LRLFRTSQE 
IIPGGEWTS RIIHLLNDQ HMGVVTAAT SLIDALVKK 
NPEEYKGCV SLAVSRLSR IVTASYTDL QDYTYYFVP 
APWLSVKLL RLLQNYTPP AEDPGVRGR LNECLETIL 
NKAQEPPKS KKVQHSNAK NAVLFEAIS LIIHNDSEA 
NLLVRACNQ LGQFLSNRE TNLRYLALE SMCHLATSE 
FSHEAVKKH QEVVILSMK MEKDVSVRQ QAVDLLYAM 
CDKSNAEEI VQEMLNYLE TADYSIREE MVLKVAILA 
EKYATDYTW YVDVILNLI RIAGDYVSE EVWYRVIQI 
VINRDEVQG YAAKTVFEA LQAPACHEN MVKVGGYIL 
GEFGNLIAG DQRSSPSVQ FQLLHSKYH LCSPMTRAL 
LLSTYIKFI NLFPEIRTQ VQEVFKQHS NLRSADAEL 
QQRASEYLQ LSIIASSDV LATVLEEMP AFPERESSI 
LAVLKKKKP GRVPENEIK ENKSPTPVT NHNNDVNNS 
SAVSGDLLG LSTPPTAQP NSGNTGVLL DVLGDIYGG 
KQANNSNSA TQPVNMYNP KKFVCKNNG VLFESDLIQ 
IGVKSEFRQ NLGRLGLFY GNKTNVPLQ NFVPTLSWP 
DDQASKLTI QMKPVEPVL EAGAQIQQM INAECVDDY 
TGAPSIVLS FLYLNVPQK ITIKLPLTI NKFFEPTEM 
NGESFFARW KNLGSTNQQ RSQKIFRAT QQMDLQAAR 
TKIMGFGMQ LLDGIDPNP DNFVCAGII HMRSQQVGC 
LLRLEPNKS AQMYRLTVR SSKESVSIE ICDLLADQF
    CDS sequence
    • >TC010798 RA
      atgccggca gtgcgagga gatggcatg cgtggcttg 
gccgtattt atttccgat ataagaaat tgtaaaagc 
aaagaagcc gagatcaag cgcattaac aaggagctc 
gcgaatata cggagcaag ttcaagggg gacaagact 
ctggatggg taccagaag aagaagtac gtgtgcaag 
ctgctcttc atattctta ctcgggcat gacattgac 
ttcgggcat atggaagct gtaaatctc ctatcatcc 
aacaaatac tccgaaaag caaatagga tatttattt 
atttcagtg ctagtcaac actaatagt gagctgatc 
aaactcata attcaaagt attaaaaat gacttggct 
tcgagaaac ccaattcac gtgaacttg gcattgcaa 
tgtattgcc aacatcggc agcagggaa atggccgaa 
gcctttggt aacgaaata ccaaaactt ctcgtctcg 
ggcgacacc atggatgta gttaagcaa agtgcagct 
ctctgtctt ctgcgtttg ttcagaacg tcgcaggaa 
atcatcccc ggaggggag tggacttca cgcatcata 
cacctcctc aatgaccaa cacatgggg gttgtgaca 
gccgcgaca agtctcata gacgcatta gttaagaag 
aaccctgag gagtacaaa ggctgcgtt tccctcgcc 
gtttcgcgc ctaagtaga atcgtgacg gcaagttac 
accgattta caagactac acatattat ttcgtacca 
gcgccctgg ctctccgtc aaactgcta cgtctcttg 
caaaattat accccgcca gctgaagac ccgggggtg 
cgcggccgc ttgaacgaa tgcctagaa acgatattg 
aataaagcg caggagcct ccaaaatcg aagaaagtg 
caacactca aatgctaaa aatgccgta ctgttcgag 
gccattagt ttaattatt cacaatgac agcgaagcg 
aatttgttg gtgcgtgct tgtaaccag ttgggccaa 
tttttgagt aatagagaa acaaattta cgttatttg 
gctttggag tcgatgtgt catttagca acgtctgaa 
ttttcccac gaggctgtt aagaaacat caggaagtt 
gttatcttg tcgatgaag atggaaaaa gatgtttca 
gtgaggcaa caagccgtt gatttgttg tacgctatg 
tgtgataag agcaatgct gaggaaatc gtccaagaa 
atgttgaat tatttggaa acggctgat tactcaatt 
agggaagaa atggtccta aaagttgcg attttggcc 
gagaaatac gccacggat tacacttgg tatgttgat 
gttatttta aatttgata agaatcgct ggcgattat 
gtgtcggaa gaggtgtgg tatcgagtg atccaaatc 
gttatcaac cgagatgaa gtgcaaggt tacgccgct 
aaaactgtc tttgaagct ttgcaagca cccgcctgt 
cacgagaac atggtcaaa gtcgggggt tacatcctg 
ggagagttt ggcaattta atcgccggg gatcagaga 
tcctcaccc tcagttcaa ttccaactt ctccactca 
aaataccac ttatgttcg ccgatgact cgagctttg 
ttactctcg acttacatc aaattcatc aatttgttc 
cccgaaata cggactcaa gtacaggaa gttttcaaa 
cagcacagc aacctgagg tcggctgac gccgaattg 
caacaacga gcttcggaa tatttgcaa ttgagtatt 
attgcaagt tcggacgtt ttggccaca gtattggag 
gaaatgccg gcgttcccc gaacgagag agctcgatt 
cttgctgtt ttgaagaag aaaaaacca ggacgggtc 
ccagaaaat gaaattaag gagaacaaa agtcccaca 
cctgtgacc aaccacaac aacgatgta aataacagc 
agtgctgtc tctggcgat ttgttgggt ctgtcgact 
ccgccaaca gcgcagccc aactccgga aacaccggg 
gtgctcctg gacgtcctt ggggacatc tacgggggc 
aaacaggcg aacaactcg aacagtgcc actcaaccg 
gtcaacatg tacaacccc aagaagttc gtgtgtaaa 
aacaacggc gttttattc gaaagtgac ttgatccag 
ataggcgtt aaaagcgag ttccgccag aatttgggc 
cgacttggc ttgttttac ggaaataag actaacgtt 
ccgttgcaa aatttcgtc ccgaccttg tcttggccc 
gacgatcag gcttccaag ctcaccatt cagatgaag 
cctgttgag ccggtcctg gaagctggt gctcaaatc 
caacagatg atcaacgcg gagtgtgtc gatgactac 
acgggcgcc cccagcata gtgctcagt ttcctgtat 
ctcaacgtg ccccagaaa ataacgatc aaacttcca 
ttgacgatt aacaagttc tttgaaccg actgaaatg 
aacggcgaa tccttcttc gcaaggtgg aaaaacctc 
ggaagtacg aaccagcaa agatctcag aagatattt 
agggcgaca cagcagatg gacttgcaa gccgcgcga 
acaaaaata atgggattc gggatgcaa ctcctggac 
gggattgat ccaaatccg gataatttc gtctgtgct 
gggattatc cacatgcgg tcgcagcaa gtcggatgt 
cttcttcgt ttagagccg aacaagagt gcacaaatg 
tacagactg acggtgcgc tctagtaaa gaaagcgtc 
tcgatagaa atttgtgat cttctagcg gatcaattt 
taa
    mRNA sequence
    • >TC010798 RA
      cgtcctgtc aatttgccc atgtatatg tggagtggc 
aaagattgg tgtgtaaaa agatatttt gttgacaat 
tgaccgtat ttatcgctt gtttgtgta gataaataa 
ttgtggaaa tcggatatt taaagaaaa gttaggagg 
attttggag gaacgaagc gatttcagc caacatgcc 
ggcagtgcg aggagatgg catgcgtgg cttggccgt 
atttatttc cgatataag aaattgtaa aagcaaaga 
agccgagat caagcgcat taacaagga gctcgcgaa 
tatacggag caagttcaa gggggacaa gactctgga 
tgggtacca gaagaagaa gtacgtgtg caagctgct 
cttcatatt cttactcgg gcatgacat tgacttcgg 
gcatatgga agctgtaaa tctcctatc atccaacaa 
atactccga aaagcaaat aggatattt atttatttc 
agtgctagt caacactaa tagtgagct gatcaaact 
cataattca aagtattaa aaatgactt ggcttcgag 
aaacccaat tcacgtgaa cttggcatt gcaatgtat 
tgccaacat cggcagcag ggaaatggc cgaagcctt 
tggtaacga aataccaaa acttctcgt ctcgggcga 
caccatgga tgtagttaa gcaaagtgc agctctctg 
tcttctgcg tttgttcag aacgtcgca ggaaatcat 
ccccggagg ggagtggac ttcacgcat catacacct 
cctcaatga ccaacacat gggggttgt gacagccgc 
gacaagtct catagacgc attagttaa gaagaaccc 
tgaggagta caaaggctg cgtttccct cgccgtttc 
gcgcctaag tagaatcgt gacggcaag ttacaccga 
tttacaaga ctacacata ttatttcgt accagcgcc 
ctggctctc cgtcaaact gctacgtct cttgcaaaa 
ttatacccc gccagctga agacccggg ggtgcgcgg 
ccgcttgaa cgaatgcct agaaacgat attgaataa 
agcgcagga gcctccaaa atcgaagaa agtgcaaca 
ctcaaatgc taaaaatgc cgtactgtt cgaggccat 
tagtttaat tattcacaa tgacagcga agcgaattt 
gttggtgcg tgcttgtaa ccagttggg ccaattttt 
gagtaatag agaaacaaa tttacgtta tttggcttt 
ggagtcgat gtgtcattt agcaacgtc tgaattttc 
ccacgaggc tgttaagaa acatcagga agttgttat 
cttgtcgat gaagatgga aaaagatgt ttcagtgag 
gcaacaagc cgttgattt gttgtacgc tatgtgtga 
taagagcaa tgctgagga aatcgtcca agaaatgtt 
gaattattt ggaaacggc tgattactc aattaggga 
agaaatggt cctaaaagt tgcgatttt ggccgagaa 
atacgccac ggattacac ttggtatgt tgatgttat 
tttaaattt gataagaat cgctggcga ttatgtgtc 
ggaagaggt gtggtatcg agtgatcca aatcgttat 
caaccgaga tgaagtgca aggttacgc cgctaaaac 
tgtctttga agctttgca agcacccgc ctgtcacga 
gaacatggt caaagtcgg gggttacat cctgggaga 
gtttggcaa tttaatcgc cggggatca gagatcctc 
accctcagt tcaattcca acttctcca ctcaaaata 
ccacttatg ttcgccgat gactcgagc tttgttact 
ctcgactta catcaaatt catcaattt gttccccga 
aatacggac tcaagtaca ggaagtttt caaacagca 
cagcaacct gaggtcggc tgacgccga attgcaaca 
acgagcttc ggaatattt gcaattgag tattattgc 
aagttcgga cgttttggc cacagtatt ggaggaaat 
gccggcgtt ccccgaacg agagagctc gattcttgc 
tgttttgaa gaagaaaaa accaggacg ggtcccaga 
aaatgaaat taaggagaa caaaagtcc cacacctgt 
gaccaacca caacaacga tgtaaataa cagcagtgc 
tgtctctgg cgatttgtt gggtctgtc gactccgcc 
aacagcgca gcccaactc cggaaacac cggggtgct 
cctggacgt ccttgggga catctacgg gggcaaaca 
ggcgaacaa ctcgaacag tgccactca accggtcaa 
catgtacaa ccccaagaa gttcgtgtg taaaaacaa 
cggcgtttt attcgaaag tgacttgat ccagatagg 
cgttaaaag cgagttccg ccagaattt gggccgact 
tggcttgtt ttacggaaa taagactaa cgttccgtt 
gcaaaattt cgtcccgac cttgtcttg gcccgacga 
tcaggcttc caagctcac cattcagat gaagcctgt 
tgagccggt cctggaagc tggtgctca aatccaaca 
gatgatcaa cgcggagtg tgtcgatga ctacacggg 
cgcccccag catagtgct cagtttcct gtatctcaa 
cgtgcccca gaaaataac gatcaaact tccattgac 
gattaacaa gttctttga accgactga aatgaacgg 
cgaatcctt cttcgcaag gtggaaaaa cctcggaag 
tacgaacca gcaaagatc tcagaagat atttagggc 
gacacagca gatggactt gcaagccgc gcgaacaaa 
aataatggg attcgggat gcaactcct ggacgggat 
tgatccaaa tccggataa tttcgtctg tgctgggat 
tatccacat gcggtcgca gcaagtcgg atgtcttct 
tcgtttaga gccgaacaa gagtgcaca aatgtacag 
actgacggt gcgctctag taaagaaag cgtctcgat 
agaaatttg tgatcttct agcggatca attttaatt 
aatttagaa aattgtgta agtaagagt gcattccat 
agtagactt accttattg tttaaaaat taatatatt 
gaaatttgc acagacatg cgctctttt ttcgtttga 
gattcgctt acagtaaaa tattagaaa gaggatgtt 
ctttgagac aatatgtaa gtgtgtgac gttagtgtt 
gcttgttga cagttaatt tatagagat atgaatttt 
ttgctgaat tcataacat tagacctct attcggaag 
aaaagggtc cattgcgga aaattaaat gtacaataa 
acgtttttt gttgagaca gatctgctg cactcgaat 
taaaccaaa aactaaggt gggtcaaac gatcttctt 
gaatgtata ttaaaaatt caatatgaa ataaaacta 
tatcattag agacgtgta acttgtgtt atattgtta 
tatcatacc agaggatgg tcgacgagt tggtaatga 
atcgttctt cagatgagg agtttattg aaacagttg 
actttctac tgtcctcag atatatttt ttaccattt 
attacaata actgattac gttgtgtag tatgccttg 
aattattat ttgtatatg ttgattgta taaataagt 
aaaatattg atttagtaa tacaaactc tttgcaatt 
ccatttttg tattcctat tttttatat catctacaa 
agtgcatga tactttatg taaaaataa tattgtgta 
atatatgaa ttcaaagct aaagttgtt gtaacatta 
tatgcgatt atttaacat ttttgtgtt tctgttgta 
ttgatgctt caattacaa aaaatcacc gtcaacatt 
tttcattac t
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