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Gene information
General information
TC010798 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 5433742..5439979
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010798
    iBeetle screen iB_01735
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010798 RA
      MPAVRGDGM RGLAVFISD IRNCKSKEA EIKRINKEL 
      ANIRSKFKG DKTLDGYQK KKYVCKLLF IFLLGHDID 
      FGHMEAVNL LSSNKYSEK QIGYLFISV LVNTNSELI 
      KLIIQSIKN DLASRNPIH VNLALQCIA NIGSREMAE 
      AFGNEIPKL LVSGDTMDV VKQSAALCL LRLFRTSQE 
      IIPGGEWTS RIIHLLNDQ HMGVVTAAT SLIDALVKK 
      NPEEYKGCV SLAVSRLSR IVTASYTDL QDYTYYFVP 
      APWLSVKLL RLLQNYTPP AEDPGVRGR LNECLETIL 
      NKAQEPPKS KKVQHSNAK NAVLFEAIS LIIHNDSEA 
      NLLVRACNQ LGQFLSNRE TNLRYLALE SMCHLATSE 
      FSHEAVKKH QEVVILSMK MEKDVSVRQ QAVDLLYAM 
      CDKSNAEEI VQEMLNYLE TADYSIREE MVLKVAILA 
      EKYATDYTW YVDVILNLI RIAGDYVSE EVWYRVIQI 
      VINRDEVQG YAAKTVFEA LQAPACHEN MVKVGGYIL 
      GEFGNLIAG DQRSSPSVQ FQLLHSKYH LCSPMTRAL 
      LLSTYIKFI NLFPEIRTQ VQEVFKQHS NLRSADAEL 
      QQRASEYLQ LSIIASSDV LATVLEEMP AFPERESSI 
      LAVLKKKKP GRVPENEIK ENKSPTPVT NHNNDVNNS 
      SAVSGDLLG LSTPPTAQP NSGNTGVLL DVLGDIYGG 
      KQANNSNSA TQPVNMYNP KKFVCKNNG VLFESDLIQ 
      IGVKSEFRQ NLGRLGLFY GNKTNVPLQ NFVPTLSWP 
      DDQASKLTI QMKPVEPVL EAGAQIQQM INAECVDDY 
      TGAPSIVLS FLYLNVPQK ITIKLPLTI NKFFEPTEM 
      NGESFFARW KNLGSTNQQ RSQKIFRAT QQMDLQAAR 
      TKIMGFGMQ LLDGIDPNP DNFVCAGII HMRSQQVGC 
      LLRLEPNKS AQMYRLTVR SSKESVSIE ICDLLADQF
    CDS sequence
    • >TC010798 RA
      atgccggca gtgcgagga gatggcatg cgtggcttg 
      gccgtattt atttccgat ataagaaat tgtaaaagc 
      aaagaagcc gagatcaag cgcattaac aaggagctc 
      gcgaatata cggagcaag ttcaagggg gacaagact 
      ctggatggg taccagaag aagaagtac gtgtgcaag 
      ctgctcttc atattctta ctcgggcat gacattgac 
      ttcgggcat atggaagct gtaaatctc ctatcatcc 
      aacaaatac tccgaaaag caaatagga tatttattt 
      atttcagtg ctagtcaac actaatagt gagctgatc 
      aaactcata attcaaagt attaaaaat gacttggct 
      tcgagaaac ccaattcac gtgaacttg gcattgcaa 
      tgtattgcc aacatcggc agcagggaa atggccgaa 
      gcctttggt aacgaaata ccaaaactt ctcgtctcg 
      ggcgacacc atggatgta gttaagcaa agtgcagct 
      ctctgtctt ctgcgtttg ttcagaacg tcgcaggaa 
      atcatcccc ggaggggag tggacttca cgcatcata 
      cacctcctc aatgaccaa cacatgggg gttgtgaca 
      gccgcgaca agtctcata gacgcatta gttaagaag 
      aaccctgag gagtacaaa ggctgcgtt tccctcgcc 
      gtttcgcgc ctaagtaga atcgtgacg gcaagttac 
      accgattta caagactac acatattat ttcgtacca 
      gcgccctgg ctctccgtc aaactgcta cgtctcttg 
      caaaattat accccgcca gctgaagac ccgggggtg 
      cgcggccgc ttgaacgaa tgcctagaa acgatattg 
      aataaagcg caggagcct ccaaaatcg aagaaagtg 
      caacactca aatgctaaa aatgccgta ctgttcgag 
      gccattagt ttaattatt cacaatgac agcgaagcg 
      aatttgttg gtgcgtgct tgtaaccag ttgggccaa 
      tttttgagt aatagagaa acaaattta cgttatttg 
      gctttggag tcgatgtgt catttagca acgtctgaa 
      ttttcccac gaggctgtt aagaaacat caggaagtt 
      gttatcttg tcgatgaag atggaaaaa gatgtttca 
      gtgaggcaa caagccgtt gatttgttg tacgctatg 
      tgtgataag agcaatgct gaggaaatc gtccaagaa 
      atgttgaat tatttggaa acggctgat tactcaatt 
      agggaagaa atggtccta aaagttgcg attttggcc 
      gagaaatac gccacggat tacacttgg tatgttgat 
      gttatttta aatttgata agaatcgct ggcgattat 
      gtgtcggaa gaggtgtgg tatcgagtg atccaaatc 
      gttatcaac cgagatgaa gtgcaaggt tacgccgct 
      aaaactgtc tttgaagct ttgcaagca cccgcctgt 
      cacgagaac atggtcaaa gtcgggggt tacatcctg 
      ggagagttt ggcaattta atcgccggg gatcagaga 
      tcctcaccc tcagttcaa ttccaactt ctccactca 
      aaataccac ttatgttcg ccgatgact cgagctttg 
      ttactctcg acttacatc aaattcatc aatttgttc 
      cccgaaata cggactcaa gtacaggaa gttttcaaa 
      cagcacagc aacctgagg tcggctgac gccgaattg 
      caacaacga gcttcggaa tatttgcaa ttgagtatt 
      attgcaagt tcggacgtt ttggccaca gtattggag 
      gaaatgccg gcgttcccc gaacgagag agctcgatt 
      cttgctgtt ttgaagaag aaaaaacca ggacgggtc 
      ccagaaaat gaaattaag gagaacaaa agtcccaca 
      cctgtgacc aaccacaac aacgatgta aataacagc 
      agtgctgtc tctggcgat ttgttgggt ctgtcgact 
      ccgccaaca gcgcagccc aactccgga aacaccggg 
      gtgctcctg gacgtcctt ggggacatc tacgggggc 
      aaacaggcg aacaactcg aacagtgcc actcaaccg 
      gtcaacatg tacaacccc aagaagttc gtgtgtaaa 
      aacaacggc gttttattc gaaagtgac ttgatccag 
      ataggcgtt aaaagcgag ttccgccag aatttgggc 
      cgacttggc ttgttttac ggaaataag actaacgtt 
      ccgttgcaa aatttcgtc ccgaccttg tcttggccc 
      gacgatcag gcttccaag ctcaccatt cagatgaag 
      cctgttgag ccggtcctg gaagctggt gctcaaatc 
      caacagatg atcaacgcg gagtgtgtc gatgactac 
      acgggcgcc cccagcata gtgctcagt ttcctgtat 
      ctcaacgtg ccccagaaa ataacgatc aaacttcca 
      ttgacgatt aacaagttc tttgaaccg actgaaatg 
      aacggcgaa tccttcttc gcaaggtgg aaaaacctc 
      ggaagtacg aaccagcaa agatctcag aagatattt 
      agggcgaca cagcagatg gacttgcaa gccgcgcga 
      acaaaaata atgggattc gggatgcaa ctcctggac 
      gggattgat ccaaatccg gataatttc gtctgtgct 
      gggattatc cacatgcgg tcgcagcaa gtcggatgt 
      cttcttcgt ttagagccg aacaagagt gcacaaatg 
      tacagactg acggtgcgc tctagtaaa gaaagcgtc 
      tcgatagaa atttgtgat cttctagcg gatcaattt 
      taa
    mRNA sequence
    • >TC010798 RA
      cgtcctgtc aatttgccc atgtatatg tggagtggc 
      aaagattgg tgtgtaaaa agatatttt gttgacaat 
      tgaccgtat ttatcgctt gtttgtgta gataaataa 
      ttgtggaaa tcggatatt taaagaaaa gttaggagg 
      attttggag gaacgaagc gatttcagc caacatgcc 
      ggcagtgcg aggagatgg catgcgtgg cttggccgt 
      atttatttc cgatataag aaattgtaa aagcaaaga 
      agccgagat caagcgcat taacaagga gctcgcgaa 
      tatacggag caagttcaa gggggacaa gactctgga 
      tgggtacca gaagaagaa gtacgtgtg caagctgct 
      cttcatatt cttactcgg gcatgacat tgacttcgg 
      gcatatgga agctgtaaa tctcctatc atccaacaa 
      atactccga aaagcaaat aggatattt atttatttc 
      agtgctagt caacactaa tagtgagct gatcaaact 
      cataattca aagtattaa aaatgactt ggcttcgag 
      aaacccaat tcacgtgaa cttggcatt gcaatgtat 
      tgccaacat cggcagcag ggaaatggc cgaagcctt 
      tggtaacga aataccaaa acttctcgt ctcgggcga 
      caccatgga tgtagttaa gcaaagtgc agctctctg 
      tcttctgcg tttgttcag aacgtcgca ggaaatcat 
      ccccggagg ggagtggac ttcacgcat catacacct 
      cctcaatga ccaacacat gggggttgt gacagccgc 
      gacaagtct catagacgc attagttaa gaagaaccc 
      tgaggagta caaaggctg cgtttccct cgccgtttc 
      gcgcctaag tagaatcgt gacggcaag ttacaccga 
      tttacaaga ctacacata ttatttcgt accagcgcc 
      ctggctctc cgtcaaact gctacgtct cttgcaaaa 
      ttatacccc gccagctga agacccggg ggtgcgcgg 
      ccgcttgaa cgaatgcct agaaacgat attgaataa 
      agcgcagga gcctccaaa atcgaagaa agtgcaaca 
      ctcaaatgc taaaaatgc cgtactgtt cgaggccat 
      tagtttaat tattcacaa tgacagcga agcgaattt 
      gttggtgcg tgcttgtaa ccagttggg ccaattttt 
      gagtaatag agaaacaaa tttacgtta tttggcttt 
      ggagtcgat gtgtcattt agcaacgtc tgaattttc 
      ccacgaggc tgttaagaa acatcagga agttgttat 
      cttgtcgat gaagatgga aaaagatgt ttcagtgag 
      gcaacaagc cgttgattt gttgtacgc tatgtgtga 
      taagagcaa tgctgagga aatcgtcca agaaatgtt 
      gaattattt ggaaacggc tgattactc aattaggga 
      agaaatggt cctaaaagt tgcgatttt ggccgagaa 
      atacgccac ggattacac ttggtatgt tgatgttat 
      tttaaattt gataagaat cgctggcga ttatgtgtc 
      ggaagaggt gtggtatcg agtgatcca aatcgttat 
      caaccgaga tgaagtgca aggttacgc cgctaaaac 
      tgtctttga agctttgca agcacccgc ctgtcacga 
      gaacatggt caaagtcgg gggttacat cctgggaga 
      gtttggcaa tttaatcgc cggggatca gagatcctc 
      accctcagt tcaattcca acttctcca ctcaaaata 
      ccacttatg ttcgccgat gactcgagc tttgttact 
      ctcgactta catcaaatt catcaattt gttccccga 
      aatacggac tcaagtaca ggaagtttt caaacagca 
      cagcaacct gaggtcggc tgacgccga attgcaaca 
      acgagcttc ggaatattt gcaattgag tattattgc 
      aagttcgga cgttttggc cacagtatt ggaggaaat 
      gccggcgtt ccccgaacg agagagctc gattcttgc 
      tgttttgaa gaagaaaaa accaggacg ggtcccaga 
      aaatgaaat taaggagaa caaaagtcc cacacctgt 
      gaccaacca caacaacga tgtaaataa cagcagtgc 
      tgtctctgg cgatttgtt gggtctgtc gactccgcc 
      aacagcgca gcccaactc cggaaacac cggggtgct 
      cctggacgt ccttgggga catctacgg gggcaaaca 
      ggcgaacaa ctcgaacag tgccactca accggtcaa 
      catgtacaa ccccaagaa gttcgtgtg taaaaacaa 
      cggcgtttt attcgaaag tgacttgat ccagatagg 
      cgttaaaag cgagttccg ccagaattt gggccgact 
      tggcttgtt ttacggaaa taagactaa cgttccgtt 
      gcaaaattt cgtcccgac cttgtcttg gcccgacga 
      tcaggcttc caagctcac cattcagat gaagcctgt 
      tgagccggt cctggaagc tggtgctca aatccaaca 
      gatgatcaa cgcggagtg tgtcgatga ctacacggg 
      cgcccccag catagtgct cagtttcct gtatctcaa 
      cgtgcccca gaaaataac gatcaaact tccattgac 
      gattaacaa gttctttga accgactga aatgaacgg 
      cgaatcctt cttcgcaag gtggaaaaa cctcggaag 
      tacgaacca gcaaagatc tcagaagat atttagggc 
      gacacagca gatggactt gcaagccgc gcgaacaaa 
      aataatggg attcgggat gcaactcct ggacgggat 
      tgatccaaa tccggataa tttcgtctg tgctgggat 
      tatccacat gcggtcgca gcaagtcgg atgtcttct 
      tcgtttaga gccgaacaa gagtgcaca aatgtacag 
      actgacggt gcgctctag taaagaaag cgtctcgat 
      agaaatttg tgatcttct agcggatca attttaatt 
      aatttagaa aattgtgta agtaagagt gcattccat 
      agtagactt accttattg tttaaaaat taatatatt 
      gaaatttgc acagacatg cgctctttt ttcgtttga 
      gattcgctt acagtaaaa tattagaaa gaggatgtt 
      ctttgagac aatatgtaa gtgtgtgac gttagtgtt 
      gcttgttga cagttaatt tatagagat atgaatttt 
      ttgctgaat tcataacat tagacctct attcggaag 
      aaaagggtc cattgcgga aaattaaat gtacaataa 
      acgtttttt gttgagaca gatctgctg cactcgaat 
      taaaccaaa aactaaggt gggtcaaac gatcttctt 
      gaatgtata ttaaaaatt caatatgaa ataaaacta 
      tatcattag agacgtgta acttgtgtt atattgtta 
      tatcatacc agaggatgg tcgacgagt tggtaatga 
      atcgttctt cagatgagg agtttattg aaacagttg 
      actttctac tgtcctcag atatatttt ttaccattt 
      attacaata actgattac gttgtgtag tatgccttg 
      aattattat ttgtatatg ttgattgta taaataagt 
      aaaatattg atttagtaa tacaaactc tttgcaatt 
      ccatttttg tattcctat tttttatat catctacaa 
      agtgcatga tactttatg taaaaataa tattgtgta 
      atatatgaa ttcaaagct aaagttgtt gtaacatta 
      tatgcgatt atttaacat ttttgtgtt tctgttgta 
      ttgatgctt caattacaa aaaatcacc gtcaacatt 
      tttcattac t