Status
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Gene information
General information
TC010784 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5210312..5236503
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC010784
      iBeetle screen iB_01733

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Fertility
      previtellogenic egg chamber not present - Number of animals showing the phenotype: 4

      vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
      Days post injection


      pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
      Days post injection



      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC010784 RA
        MAATGTATT AQQRRSFSW LGPGPVLHA ALLVLFGSA 
        AFVAVAANQ LQPNSDLAH QNEGEICES VDIRNTLDS 
        FNRLKGCHV VEGFVQILL FDNVNETEL SLLSFPNLT 
        EITDYLLLY RVNGLRSIG QLFPNLSVI RGRSTFYTY 
        SFVVFEMSS LQEIGLYSL TDITRGLIR IDKNPSLCF 
        VNSIDWEAI AHEKGEHFI KNLKSPNEC PICPGDEKE 
        DESGNSVAH IHCHKAPHR SDSENFDRD VHLCWNRQH 
        CQKICPTKC KHSCNANLE CCDESCLGG CSINDTKLC 
        TVCKNLSMG FGAKKQCMS SCPPDYYQY LERRCILKN 
        ECKDMKRPL NFQQQGGPE KPFKIFNNS CILECPPNY 
        MSNDTHCIP CQGNCIKKC AGVNVDSIN LARQLKGCT 
        HITSSLEIQ IRGGRNVVN ELEESLGMI EEIDGYLKV 
        VRSFPLVSL NFLKNLKVI HGRQLESQK YVFVVLDNQ 
        NLQELWNWE NNKTLKIDT GRLFFHFNP KLCIREIEK 
        LQNITHIVD VTELEVAKN SNGDKIACE LQVLKVDKP 
        KVVNSKGVV LEWEPFKID DPRKLLGYI VYSIEAPTQ 
        NVTLYDGRD ACGGDGWRV DDVAIPENE MNVTHPLTS 
        LKPYTQYAF YVKTYTIAT ERRGAQSMI SYFTTLPDT 
        PTAPVGLEV VSNSSNSLK LSWRPPKSP NGKLTHYIV 
        SLKRHNSTT PDLDEMEHF CKSPSSRPK HPIPSVSVP 
        PVTSTTTSN DTCQCLETK PSTSSINED VEKSRIDFE 
        DELHNAVYV RKPNFSESR KRRDVDSEQ LNANRVFNE 
        TDEAGAYIS FSTVVTGTE FYMPNLRHY TVYEINVQA 
        CREKTNDKL DTENPCSSK NMKTHRTLK KKGADDIKQ 
        IEVKNQSLG MVSLTWKEP EAPNGLIYC YTIEYKKLE 
        IENSKANEE YITHARFIN QSRIYTLKA LSPGNYSVR 
        VSATTSGDY ANFSPYAYF YIEERPSNT YVTLIVCML 
        ILVIILALC AFCFYKKKK ADKESMRLI PSVNPEYVP 
        SVYVPDEWE VPRKKIELI RELGQGSFG MVYEGIAQD 
        VRGKAQIKC AVKTVNEHA TNRERLEFL NEASVMKAF 
        DTAHVVRLL GVVSQGQPT LVIMELMAN GDLKTYLRS 
        HRPDAEVYD PATAKQPPT LKQILQMAI EIADGMAYL 
        SAKKFVHRD LAARNCMVA EDLTVKIGD FGMTRDIYE 
        TDYYRKGTK GLLPVRWMA PESLKDGVF TSNSDVWSY 
        GVVLWEMAT LASQPYQGL SNDQVLRYV IDGGVMERP 
        ENCPDKLYT LMRYCWQHK PSARPSFLK LCSLLLEDA 
        STSFAQVSF YHSAAGIEA RSSRPTPSP SQDDPSTPL 
        RIAGDHDVN FSLNSDDSN DEFETDAET HIRFPSIPV 
        ENKDGITTA NGYVSGCPT NGAATTQC
      CDS sequence
      • >TC010784 RA
        atggccgcg accggcacc gccacaacg gcccaacag 
        cgccgctcc ttcagctgg ctcggccca gggccggtg 
        ctccacgcc gccctgctc gtcctcttc ggcagcgct 
        gccttcgtc gccgtcgcc gcgaaccaa ctccagccc 
        aattctgac ctcgcgcac cagaatgaa ggtgaaatt 
        tgtgaaagc gtggatatt agaaatacc ctggattcg 
        ttcaacagg ttaaaaggt tgccacgtg gtggaaggt 
        tttgtgcag attttactt tttgataat gtgaacgag 
        actgagtta tccctttta agttttcca aacctaact 
        gaaataacc gattattta ttactgtac agagtgaac 
        ggattgcga agtatagga cagttgttt ccgaatttg 
        agcgtcatt cgaggacgc tcaacgttt tacacttat 
        tcattcgtc gtatttgaa atgtctagc ttacaagaa 
        ataggtcta tattcactg actgacatt actcgcgga 
        ctaatcagg attgataaa aatccttct ctgtgcttc 
        gtgaactcg atcgactgg gaagctata gcccacgaa 
        aagggagaa cattttatt aaaaaccta aagtcacca 
        aatgaatgc ccgatctgt cccggtgac gaaaaggag 
        gacgagtcg ggcaatagc gtggcacat attcattgc 
        cataaagca cctcaccgg tccgacagc gaaaatttc 
        gatagagac gtccatctc tgttggaat cgacagcac 
        tgtcagaag atctgccct acgaagtgc aaacactcc 
        tgtaacgcc aatttggaa tgctgcgac gagagctgt 
        ctaggagga tgtagtata aatgatacc aaactttgc 
        actgtttgt aaaaatctc agcatgggg tttggggca 
        aagaaacaa tgcatgtcg agttgtccc cccgattat 
        tatcagtat ttagaaaga cgctgtatc ttgaagaat 
        gaatgcaaa gatatgaag agacctctg aattttcag 
        cagcagggt gggcccgaa aaacctttc aaaattttc 
        aacaattcc tgtatttta gaatgtcca cccaattat 
        atgagtaac gacactcat tgtataccg tgtcaaggg 
        aattgtata aaaaagtgt gcgggagtg aatgttgat 
        agtataaat ttagcaaga cagttgaaa gggtgcacc 
        catataaca agttctcta gaaatacaa attcggggc 
        ggccgaaat gtagtgaat gaattggag gaaagtcta 
        ggcatgatt gaagaaatc gacggttat ttgaaagta 
        gtcagatca tttcctttg gtttcatta aactttttg 
        aaaaatttg aaagtgata cacggcaga cagttagaa 
        agccaaaaa tacgttttc gttgtactt gataatcaa 
        aatcttcaa gaactgtgg aactgggaa aataataaa 
        acgcttaaa atagacacc ggacgttta ttctttcac 
        ttcaaccca aaactttgc atcagagaa atcgaaaag 
        ttacaaaat atcacccac atcgtcgat gttactgaa 
        ttagaagtg gcgaaaaac tcgaacggc gataaaatt 
        gcatgtgaa cttcaagtt ttaaaagta gataaaccc 
        aaagtggtg aatagcaaa ggcgtcgtc ttggaatgg 
        gagccgttc aaaatcgac gatccgcga aaactttta 
        ggctacatt gtctactcg atcgaagca ccaacccag 
        aacgttacg ctctatgat ggacgagat gcttgtgga 
        ggagacgga tggcgcgtt gatgatgtt gcaattcct 
        gaaaacgaa atgaatgtt acacatcca cttacatcg 
        ttaaaaccc tacacacag tacgcattt tatgtcaaa 
        acttacacg atagctact gaaagaaga ggtgcacaa 
        agtatgatt tcgtacttc actacgttg cccgatact 
        ccaacggca ccggttggc ttagaggta gttagtaat 
        tcgagcaac tctcttaag ttatcgtgg aggcctccc 
        aaatctccc aacggcaaa cttactcac tacattgtt 
        agtttgaaa aggcacaac agtactact cccgatctg 
        gatgaaatg gagcatttc tgcaaatca ccctcgagt 
        cgcccaaaa caccccatt cctagtgtg agcgttcct 
        ccagttact tcaacgaca acaagtaac gacacttgc 
        cagtgttta gaaactaaa ccgtcaaca tcatcaatt 
        aatgaagat gtcgaaaaa tcccgaatt gattttgaa 
        gatgaatta cataacgcg gtttatgtg agaaagccg 
        aatttttca gaatctcgc aaaagacgt gacgtggac 
        agtgagcaa ctaaatgcg aaccgggtg tttaatgaa 
        accgatgaa gccggtgct tatatttcg ttcagtact 
        gtagtcacg gggacagaa ttttacatg ccgaatttg 
        cggcactat acggtttat gaaataaac gtccaagcc 
        tgtagagaa aaaaccaac gacaagctt gataccgaa 
        aatccctgc agtagcaaa aatatgaaa acgcatcgg 
        actctgaag aagaaagga gccgatgat ataaaacaa 
        attgaagtc aaaaaccaa agtttggga atggtgtca 
        cttacgtgg aaggaaccg gaggctccg aacggattg 
        atctattgt tacacgata gaatataaa aagttggaa 
        attgaaaat tccaaggct aatgaagag tatattaca 
        cacgcgcgt tttatcaac cagagtagg atttatacc 
        ttgaaagcg ttatcgcct ggaaattac agtgttcga 
        gtttctgca acgacgtca ggtgattac gccaacttt 
        tctccttac gcgtacttt tatatcgag gaacggccg 
        tctaacact tacgtaacc ttgattgtc tgtatgctc 
        attctggtg ataattttg gcactctgt gcgttttgt 
        ttttacaag aagaagaaa gccgacaag gagagtatg 
        cggctgatt ccgtcggtc aatcccgag tatgttcca 
        agtgtgtat gttccggac gaatgggag gtgcctcgc 
        aaaaaaata gaactgatt agggagtta ggacagggg 
        agttttggc atggtatac gagggcatc gcccaagac 
        gtccgcgga aaggcgcaa atcaaatgc gctgtgaag 
        actgttaat gagcacgcc actaatagg gagcgtttg 
        gagtttttg aacgaagct tccgtcatg aaggctttc 
        gatactgct catgtcgtg aggttgttg ggagtggtg 
        tcgcaaggg cagcccacg ttggtgatc atggagctg 
        atggcgaat ggcgacttg aagacctac ctgcgttcc 
        caccgtcca gacgccgaa gtgtatgac cctgccacg 
        gccaagcaa cccccgacg ctgaagcag atcctgcaa 
        atggccata gaaatagcc gacggaatg gcctacctg 
        tcggccaag aagttcgtc caccgcgac ctggcagct 
        agaaattgc atggtagct gaagacttg actgttaaa 
        ataggtgat tttggtatg acgcgtgat atttacgaa 
        acggactac tatcgcaag ggcactaag gggctgctc 
        cccgtgcga tggatggcc cccgagagt ctcaaagac 
        ggtgtgttt actagtaat agtgatgtt tggagttac 
        ggagttgtg ttgtgggag atggcgact ctggcgtcg 
        cagccgtat caaggcttg tcgaacgac caggttttg 
        cgatatgtg attgacgga ggtgtgatg gagcggccg 
        gagaactgt cccgataaa ctgtacact ttgatgaga 
        tactgttgg caacacaaa ccatcagca cgtccttcg 
        tttttaaag ttatgctca ttgttgtta gaggatgcc 
        agcactagt tttgctcag gtgtcgttt tatcatagt 
        gcggctgga attgaggct cgatcgtcg cggccgaca 
        ccgtcgccg tcgcaggat gatccgagt actccgctc 
        cggatagct ggagaccat gatgtcaat ttttctctc 
        aattcggac gactcgaat gacgagttc gaaacagac 
        gctgagact catattagg tttcccagt attcccgtg 
        gagaacaaa gacggaatc accaccgct aacggttac 
        gtgagtgga tgtcccaca aatggtgca gcaacaact 
        cagtgctag
      mRNA sequence
      • >TC010784 RA
        atgcgcagg agaagttaa aggcattgt tgggtggag 
        gtgaaaata cactacccg ttcggattc gttcgtatt 
        ttcccatgt tgcctatgt agcgcagta gcgtgtata 
        agctgcgtt gaatttaaa aataaacgg agtaagata 
        aaacgcata gtgtttgat tgatggttt tcgtaaggt 
        gatggtatt ctatgaatg gcatcgaat tatttatta 
        gtgaaaggt ctgaggatt tttgacgga gtggcaacg 
        ttgttcaac gcaatcgcc aaacaaata ttagtaaag 
        ttgtatgta accaactcc agtaagcat ggtggataa 
        agtgtatac gacgttgtt tagtggatt tgttgtgca 
        ataaaatcg cctttggtt tttaattgt tcgggtagt 
        ggagtttga attggtttc gggtggcgt tgtcagtgc 
        tctgtgcgt tatgtttcg gtaaaaaat gaacctgag 
        gatggtgcg ctttttcaa ggtgtaacc tcgagcgag 
        caccggcag ttggttttt ggacatccc ggccgaagt 
        gaataacat gtttacatt gatcgagca ctgcgtcat 
        aagtttaca gaaattctg tacgacgcg agttggcga 
        accttgcac gggccccag cagacccat gtctatgta 
        ttgccactg atggccgcg accggcacc gccacaacg 
        gcccaacag cgccgctcc ttcagctgg ctcggccca 
        gggccggtg ctccacgcc gccctgctc gtcctcttc 
        ggcagcgct gccttcgtc gccgtcgcc gcgaaccaa 
        ctccagccc aattctgac ctcgcgcac cagaatgaa 
        ggtgaaatt tgtgaaagc gtggatatt agaaatacc 
        ctggattcg ttcaacagg ttaaaaggt tgccacgtg 
        gtggaaggt tttgtgcag attttactt tttgataat 
        gtgaacgag actgagtta tccctttta agttttcca 
        aacctaact gaaataacc gattattta ttactgtac 
        agagtgaac ggattgcga agtatagga cagttgttt 
        ccgaatttg agcgtcatt cgaggacgc tcaacgttt 
        tacacttat tcattcgtc gtatttgaa atgtctagc 
        ttacaagaa ataggtcta tattcactg actgacatt 
        actcgcgga ctaatcagg attgataaa aatccttct 
        ctgtgcttc gtgaactcg atcgactgg gaagctata 
        gcccacgaa aagggagaa cattttatt aaaaaccta 
        aagtcacca aatgaatgc ccgatctgt cccggtgac 
        gaaaaggag gacgagtcg ggcaatagc gtggcacat 
        attcattgc cataaagca cctcaccgg tccgacagc 
        gaaaatttc gatagagac gtccatctc tgttggaat 
        cgacagcac tgtcagaag atctgccct acgaagtgc 
        aaacactcc tgtaacgcc aatttggaa tgctgcgac 
        gagagctgt ctaggagga tgtagtata aatgatacc 
        aaactttgc actgtttgt aaaaatctc agcatgggg 
        tttggggca aagaaacaa tgcatgtcg agttgtccc 
        cccgattat tatcagtat ttagaaaga cgctgtatc 
        ttgaagaat gaatgcaaa gatatgaag agacctctg 
        aattttcag cagcagggt gggcccgaa aaacctttc 
        aaaattttc aacaattcc tgtatttta gaatgtcca 
        cccaattat atgagtaac gacactcat tgtataccg 
        tgtcaaggg aattgtata aaaaagtgt gcgggagtg 
        aatgttgat agtataaat ttagcaaga cagttgaaa 
        gggtgcacc catataaca agttctcta gaaatacaa 
        attcggggc ggccgaaat gtagtgaat gaattggag 
        gaaagtcta ggcatgatt gaagaaatc gacggttat 
        ttgaaagta gtcagatca tttcctttg gtttcatta 
        aactttttg aaaaatttg aaagtgata cacggcaga 
        cagttagaa agccaaaaa tacgttttc gttgtactt 
        gataatcaa aatcttcaa gaactgtgg aactgggaa 
        aataataaa acgcttaaa atagacacc ggacgttta 
        ttctttcac ttcaaccca aaactttgc atcagagaa 
        atcgaaaag ttacaaaat atcacccac atcgtcgat 
        gttactgaa ttagaagtg gcgaaaaac tcgaacggc 
        gataaaatt gcatgtgaa cttcaagtt ttaaaagta 
        gataaaccc aaagtggtg aatagcaaa ggcgtcgtc 
        ttggaatgg gagccgttc aaaatcgac gatccgcga 
        aaactttta ggctacatt gtctactcg atcgaagca 
        ccaacccag aacgttacg ctctatgat ggacgagat 
        gcttgtgga ggagacgga tggcgcgtt gatgatgtt 
        gcaattcct gaaaacgaa atgaatgtt acacatcca 
        cttacatcg ttaaaaccc tacacacag tacgcattt 
        tatgtcaaa acttacacg atagctact gaaagaaga 
        ggtgcacaa agtatgatt tcgtacttc actacgttg 
        cccgatact ccaacggca ccggttggc ttagaggta 
        gttagtaat tcgagcaac tctcttaag ttatcgtgg 
        aggcctccc aaatctccc aacggcaaa cttactcac 
        tacattgtt agtttgaaa aggcacaac agtactact 
        cccgatctg gatgaaatg gagcatttc tgcaaatca 
        ccctcgagt cgcccaaaa caccccatt cctagtgtg 
        agcgttcct ccagttact tcaacgaca acaagtaac 
        gacacttgc cagtgttta gaaactaaa ccgtcaaca 
        tcatcaatt aatgaagat gtcgaaaaa tcccgaatt 
        gattttgaa gatgaatta cataacgcg gtttatgtg 
        agaaagccg aatttttca gaatctcgc aaaagacgt 
        gacgtggac agtgagcaa ctaaatgcg aaccgggtg 
        tttaatgaa accgatgaa gccggtgct tatatttcg 
        ttcagtact gtagtcacg gggacagaa ttttacatg 
        ccgaatttg cggcactat acggtttat gaaataaac 
        gtccaagcc tgtagagaa aaaaccaac gacaagctt 
        gataccgaa aatccctgc agtagcaaa aatatgaaa 
        acgcatcgg actctgaag aagaaagga gccgatgat 
        ataaaacaa attgaagtc aaaaaccaa agtttggga 
        atggtgtca cttacgtgg aaggaaccg gaggctccg 
        aacggattg atctattgt tacacgata gaatataaa 
        aagttggaa attgaaaat tccaaggct aatgaagag 
        tatattaca cacgcgcgt tttatcaac cagagtagg 
        atttatacc ttgaaagcg ttatcgcct ggaaattac 
        agtgttcga gtttctgca acgacgtca ggtgattac 
        gccaacttt tctccttac gcgtacttt tatatcgag 
        gaacggccg tctaacact tacgtaacc ttgattgtc 
        tgtatgctc attctggtg ataattttg gcactctgt 
        gcgttttgt ttttacaag aagaagaaa gccgacaag 
        gagagtatg cggctgatt ccgtcggtc aatcccgag 
        tatgttcca agtgtgtat gttccggac gaatgggag 
        gtgcctcgc aaaaaaata gaactgatt agggagtta 
        ggacagggg agttttggc atggtatac gagggcatc 
        gcccaagac gtccgcgga aaggcgcaa atcaaatgc 
        gctgtgaag actgttaat gagcacgcc actaatagg 
        gagcgtttg gagtttttg aacgaagct tccgtcatg 
        aaggctttc gatactgct catgtcgtg aggttgttg 
        ggagtggtg tcgcaaggg cagcccacg ttggtgatc 
        atggagctg atggcgaat ggcgacttg aagacctac 
        ctgcgttcc caccgtcca gacgccgaa gtgtatgac 
        cctgccacg gccaagcaa cccccgacg ctgaagcag 
        atcctgcaa atggccata gaaatagcc gacggaatg 
        gcctacctg tcggccaag aagttcgtc caccgcgac 
        ctggcagct agaaattgc atggtagct gaagacttg 
        actgttaaa ataggtgat tttggtatg acgcgtgat 
        atttacgaa acggactac tatcgcaag ggcactaag 
        gggctgctc cccgtgcga tggatggcc cccgagagt 
        ctcaaagac ggtgtgttt actagtaat agtgatgtt 
        tggagttac ggagttgtg ttgtgggag atggcgact 
        ctggcgtcg cagccgtat caaggcttg tcgaacgac 
        caggttttg cgatatgtg attgacgga ggtgtgatg 
        gagcggccg gagaactgt cccgataaa ctgtacact 
        ttgatgaga tactgttgg caacacaaa ccatcagca 
        cgtccttcg tttttaaag ttatgctca ttgttgtta 
        gaggatgcc agcactagt tttgctcag gtgtcgttt 
        tatcatagt gcggctgga attgaggct cgatcgtcg 
        cggccgaca ccgtcgccg tcgcaggat gatccgagt 
        actccgctc cggatagct ggagaccat gatgtcaat 
        ttttctctc aattcggac gactcgaat gacgagttc 
        gaaacagac gctgagact catattagg tttcccagt 
        attcccgtg gagaacaaa gacggaatc accaccgct 
        aacggttac gtgagtgga tgtcccaca aatggtgca 
        gcaacaact cagtgctag ctacatatc acatctcag 
        ttgaaagta tttcctata aagatctga ctattgcag 
        caagagaca taaataagt aacatgact gtttttgta 
        aagtagtta taagtttaa attaccaaa gatattttc 
        ttcatttgc tgaaaggcc tacatatta cagaaaaac 
        agtgttcat tagcaaatc attaacaag tgaggcgta 
        ttcagtgag cctgaaacc ataaagact agtctattg 
        attttccat tctatggga tactctaag attcttgtc 
        tactcaata ttatgtgta aaagagatg aaactaatt 
        gccttatta gttaaagta attataatg tgtagcatc 
        ccataaaga cattctttg gacaattgc caatcaata 
        cattgcagc ctgactctg agttcctgt tatcttatt 
        gatgattgg tagtcacaa ctactttat tgtacctat 
        ttatatttt agttaaatt ttgtactct gtaatataa 
        tgtacatta tttc
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