| General information | |||
| TC010784 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 5210312..5236503 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC010784 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01733 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
- >TC010784 RA
MAATGTATT AQQRRSFSW LGPGPVLHA ALLVLFGSA AFVAVAANQ LQPNSDLAH QNEGEICES VDIRNTLDS FNRLKGCHV VEGFVQILL FDNVNETEL SLLSFPNLT EITDYLLLY RVNGLRSIG QLFPNLSVI RGRSTFYTY SFVVFEMSS LQEIGLYSL TDITRGLIR IDKNPSLCF VNSIDWEAI AHEKGEHFI KNLKSPNEC PICPGDEKE DESGNSVAH IHCHKAPHR SDSENFDRD VHLCWNRQH CQKICPTKC KHSCNANLE CCDESCLGG CSINDTKLC TVCKNLSMG FGAKKQCMS SCPPDYYQY LERRCILKN ECKDMKRPL NFQQQGGPE KPFKIFNNS CILECPPNY MSNDTHCIP CQGNCIKKC AGVNVDSIN LARQLKGCT HITSSLEIQ IRGGRNVVN ELEESLGMI EEIDGYLKV VRSFPLVSL NFLKNLKVI HGRQLESQK YVFVVLDNQ NLQELWNWE NNKTLKIDT GRLFFHFNP KLCIREIEK LQNITHIVD VTELEVAKN SNGDKIACE LQVLKVDKP KVVNSKGVV LEWEPFKID DPRKLLGYI VYSIEAPTQ NVTLYDGRD ACGGDGWRV DDVAIPENE MNVTHPLTS LKPYTQYAF YVKTYTIAT ERRGAQSMI SYFTTLPDT PTAPVGLEV VSNSSNSLK LSWRPPKSP NGKLTHYIV SLKRHNSTT PDLDEMEHF CKSPSSRPK HPIPSVSVP PVTSTTTSN DTCQCLETK PSTSSINED VEKSRIDFE DELHNAVYV RKPNFSESR KRRDVDSEQ LNANRVFNE TDEAGAYIS FSTVVTGTE FYMPNLRHY TVYEINVQA CREKTNDKL DTENPCSSK NMKTHRTLK KKGADDIKQ IEVKNQSLG MVSLTWKEP EAPNGLIYC YTIEYKKLE IENSKANEE YITHARFIN QSRIYTLKA LSPGNYSVR VSATTSGDY ANFSPYAYF YIEERPSNT YVTLIVCML ILVIILALC AFCFYKKKK ADKESMRLI PSVNPEYVP SVYVPDEWE VPRKKIELI RELGQGSFG MVYEGIAQD VRGKAQIKC AVKTVNEHA TNRERLEFL NEASVMKAF DTAHVVRLL GVVSQGQPT LVIMELMAN GDLKTYLRS HRPDAEVYD PATAKQPPT LKQILQMAI EIADGMAYL SAKKFVHRD LAARNCMVA EDLTVKIGD FGMTRDIYE TDYYRKGTK GLLPVRWMA PESLKDGVF TSNSDVWSY GVVLWEMAT LASQPYQGL SNDQVLRYV IDGGVMERP ENCPDKLYT LMRYCWQHK PSARPSFLK LCSLLLEDA STSFAQVSF YHSAAGIEA RSSRPTPSP SQDDPSTPL RIAGDHDVN FSLNSDDSN DEFETDAET HIRFPSIPV ENKDGITTA NGYVSGCPT NGAATTQC
- >TC010784 RA
atggccgcg accggcacc gccacaacg gcccaacag cgccgctcc ttcagctgg ctcggccca gggccggtg ctccacgcc gccctgctc gtcctcttc ggcagcgct gccttcgtc gccgtcgcc gcgaaccaa ctccagccc aattctgac ctcgcgcac cagaatgaa ggtgaaatt tgtgaaagc gtggatatt agaaatacc ctggattcg ttcaacagg ttaaaaggt tgccacgtg gtggaaggt tttgtgcag attttactt tttgataat gtgaacgag actgagtta tccctttta agttttcca aacctaact gaaataacc gattattta ttactgtac agagtgaac ggattgcga agtatagga cagttgttt ccgaatttg agcgtcatt cgaggacgc tcaacgttt tacacttat tcattcgtc gtatttgaa atgtctagc ttacaagaa ataggtcta tattcactg actgacatt actcgcgga ctaatcagg attgataaa aatccttct ctgtgcttc gtgaactcg atcgactgg gaagctata gcccacgaa aagggagaa cattttatt aaaaaccta aagtcacca aatgaatgc ccgatctgt cccggtgac gaaaaggag gacgagtcg ggcaatagc gtggcacat attcattgc cataaagca cctcaccgg tccgacagc gaaaatttc gatagagac gtccatctc tgttggaat cgacagcac tgtcagaag atctgccct acgaagtgc aaacactcc tgtaacgcc aatttggaa tgctgcgac gagagctgt ctaggagga tgtagtata aatgatacc aaactttgc actgtttgt aaaaatctc agcatgggg tttggggca aagaaacaa tgcatgtcg agttgtccc cccgattat tatcagtat ttagaaaga cgctgtatc ttgaagaat gaatgcaaa gatatgaag agacctctg aattttcag cagcagggt gggcccgaa aaacctttc aaaattttc aacaattcc tgtatttta gaatgtcca cccaattat atgagtaac gacactcat tgtataccg tgtcaaggg aattgtata aaaaagtgt gcgggagtg aatgttgat agtataaat ttagcaaga cagttgaaa gggtgcacc catataaca agttctcta gaaatacaa attcggggc ggccgaaat gtagtgaat gaattggag gaaagtcta ggcatgatt gaagaaatc gacggttat ttgaaagta gtcagatca tttcctttg gtttcatta aactttttg aaaaatttg aaagtgata cacggcaga cagttagaa agccaaaaa tacgttttc gttgtactt gataatcaa aatcttcaa gaactgtgg aactgggaa aataataaa acgcttaaa atagacacc ggacgttta ttctttcac ttcaaccca aaactttgc atcagagaa atcgaaaag ttacaaaat atcacccac atcgtcgat gttactgaa ttagaagtg gcgaaaaac tcgaacggc gataaaatt gcatgtgaa cttcaagtt ttaaaagta gataaaccc aaagtggtg aatagcaaa ggcgtcgtc ttggaatgg gagccgttc aaaatcgac gatccgcga aaactttta ggctacatt gtctactcg atcgaagca ccaacccag aacgttacg ctctatgat ggacgagat gcttgtgga ggagacgga tggcgcgtt gatgatgtt gcaattcct gaaaacgaa atgaatgtt acacatcca cttacatcg ttaaaaccc tacacacag tacgcattt tatgtcaaa acttacacg atagctact gaaagaaga ggtgcacaa agtatgatt tcgtacttc actacgttg cccgatact ccaacggca ccggttggc ttagaggta gttagtaat tcgagcaac tctcttaag ttatcgtgg aggcctccc aaatctccc aacggcaaa cttactcac tacattgtt agtttgaaa aggcacaac agtactact cccgatctg gatgaaatg gagcatttc tgcaaatca ccctcgagt cgcccaaaa caccccatt cctagtgtg agcgttcct ccagttact tcaacgaca acaagtaac gacacttgc cagtgttta gaaactaaa ccgtcaaca tcatcaatt aatgaagat gtcgaaaaa tcccgaatt gattttgaa gatgaatta cataacgcg gtttatgtg agaaagccg aatttttca gaatctcgc aaaagacgt gacgtggac agtgagcaa ctaaatgcg aaccgggtg tttaatgaa accgatgaa gccggtgct tatatttcg ttcagtact gtagtcacg gggacagaa ttttacatg ccgaatttg cggcactat acggtttat gaaataaac gtccaagcc tgtagagaa aaaaccaac gacaagctt gataccgaa aatccctgc agtagcaaa aatatgaaa acgcatcgg actctgaag aagaaagga gccgatgat ataaaacaa attgaagtc aaaaaccaa agtttggga atggtgtca cttacgtgg aaggaaccg gaggctccg aacggattg atctattgt tacacgata gaatataaa aagttggaa attgaaaat tccaaggct aatgaagag tatattaca cacgcgcgt tttatcaac cagagtagg atttatacc ttgaaagcg ttatcgcct ggaaattac agtgttcga gtttctgca acgacgtca ggtgattac gccaacttt tctccttac gcgtacttt tatatcgag gaacggccg tctaacact tacgtaacc ttgattgtc tgtatgctc attctggtg ataattttg gcactctgt gcgttttgt ttttacaag aagaagaaa gccgacaag gagagtatg cggctgatt ccgtcggtc aatcccgag tatgttcca agtgtgtat gttccggac gaatgggag gtgcctcgc aaaaaaata gaactgatt agggagtta ggacagggg agttttggc atggtatac gagggcatc gcccaagac gtccgcgga aaggcgcaa atcaaatgc gctgtgaag actgttaat gagcacgcc actaatagg gagcgtttg gagtttttg aacgaagct tccgtcatg aaggctttc gatactgct catgtcgtg aggttgttg ggagtggtg tcgcaaggg cagcccacg ttggtgatc atggagctg atggcgaat ggcgacttg aagacctac ctgcgttcc caccgtcca gacgccgaa gtgtatgac cctgccacg gccaagcaa cccccgacg ctgaagcag atcctgcaa atggccata gaaatagcc gacggaatg gcctacctg tcggccaag aagttcgtc caccgcgac ctggcagct agaaattgc atggtagct gaagacttg actgttaaa ataggtgat tttggtatg acgcgtgat atttacgaa acggactac tatcgcaag ggcactaag gggctgctc cccgtgcga tggatggcc cccgagagt ctcaaagac ggtgtgttt actagtaat agtgatgtt tggagttac ggagttgtg ttgtgggag atggcgact ctggcgtcg cagccgtat caaggcttg tcgaacgac caggttttg cgatatgtg attgacgga ggtgtgatg gagcggccg gagaactgt cccgataaa ctgtacact ttgatgaga tactgttgg caacacaaa ccatcagca cgtccttcg tttttaaag ttatgctca ttgttgtta gaggatgcc agcactagt tttgctcag gtgtcgttt tatcatagt gcggctgga attgaggct cgatcgtcg cggccgaca ccgtcgccg tcgcaggat gatccgagt actccgctc cggatagct ggagaccat gatgtcaat ttttctctc aattcggac gactcgaat gacgagttc gaaacagac gctgagact catattagg tttcccagt attcccgtg gagaacaaa gacggaatc accaccgct aacggttac gtgagtgga tgtcccaca aatggtgca gcaacaact cagtgctag
- >TC010784 RA
atgcgcagg agaagttaa aggcattgt tgggtggag gtgaaaata cactacccg ttcggattc gttcgtatt ttcccatgt tgcctatgt agcgcagta gcgtgtata agctgcgtt gaatttaaa aataaacgg agtaagata aaacgcata gtgtttgat tgatggttt tcgtaaggt gatggtatt ctatgaatg gcatcgaat tatttatta gtgaaaggt ctgaggatt tttgacgga gtggcaacg ttgttcaac gcaatcgcc aaacaaata ttagtaaag ttgtatgta accaactcc agtaagcat ggtggataa agtgtatac gacgttgtt tagtggatt tgttgtgca ataaaatcg cctttggtt tttaattgt tcgggtagt ggagtttga attggtttc gggtggcgt tgtcagtgc tctgtgcgt tatgtttcg gtaaaaaat gaacctgag gatggtgcg ctttttcaa ggtgtaacc tcgagcgag caccggcag ttggttttt ggacatccc ggccgaagt gaataacat gtttacatt gatcgagca ctgcgtcat aagtttaca gaaattctg tacgacgcg agttggcga accttgcac gggccccag cagacccat gtctatgta ttgccactg atggccgcg accggcacc gccacaacg gcccaacag cgccgctcc ttcagctgg ctcggccca gggccggtg ctccacgcc gccctgctc gtcctcttc ggcagcgct gccttcgtc gccgtcgcc gcgaaccaa ctccagccc aattctgac ctcgcgcac cagaatgaa ggtgaaatt tgtgaaagc gtggatatt agaaatacc ctggattcg ttcaacagg ttaaaaggt tgccacgtg gtggaaggt tttgtgcag attttactt tttgataat gtgaacgag actgagtta tccctttta agttttcca aacctaact gaaataacc gattattta ttactgtac agagtgaac ggattgcga agtatagga cagttgttt ccgaatttg agcgtcatt cgaggacgc tcaacgttt tacacttat tcattcgtc gtatttgaa atgtctagc ttacaagaa ataggtcta tattcactg actgacatt actcgcgga ctaatcagg attgataaa aatccttct ctgtgcttc gtgaactcg atcgactgg gaagctata gcccacgaa aagggagaa cattttatt aaaaaccta aagtcacca aatgaatgc ccgatctgt cccggtgac gaaaaggag gacgagtcg ggcaatagc gtggcacat attcattgc cataaagca cctcaccgg tccgacagc gaaaatttc gatagagac gtccatctc tgttggaat cgacagcac tgtcagaag atctgccct acgaagtgc aaacactcc tgtaacgcc aatttggaa tgctgcgac gagagctgt ctaggagga tgtagtata aatgatacc aaactttgc actgtttgt aaaaatctc agcatgggg tttggggca aagaaacaa tgcatgtcg agttgtccc cccgattat tatcagtat ttagaaaga cgctgtatc ttgaagaat gaatgcaaa gatatgaag agacctctg aattttcag cagcagggt gggcccgaa aaacctttc aaaattttc aacaattcc tgtatttta gaatgtcca cccaattat atgagtaac gacactcat tgtataccg tgtcaaggg aattgtata aaaaagtgt gcgggagtg aatgttgat agtataaat ttagcaaga cagttgaaa gggtgcacc catataaca agttctcta gaaatacaa attcggggc ggccgaaat gtagtgaat gaattggag gaaagtcta ggcatgatt gaagaaatc gacggttat ttgaaagta gtcagatca tttcctttg gtttcatta aactttttg aaaaatttg aaagtgata cacggcaga cagttagaa agccaaaaa tacgttttc gttgtactt gataatcaa aatcttcaa gaactgtgg aactgggaa aataataaa acgcttaaa atagacacc ggacgttta ttctttcac ttcaaccca aaactttgc atcagagaa atcgaaaag ttacaaaat atcacccac atcgtcgat gttactgaa ttagaagtg gcgaaaaac tcgaacggc gataaaatt gcatgtgaa cttcaagtt ttaaaagta gataaaccc aaagtggtg aatagcaaa ggcgtcgtc ttggaatgg gagccgttc aaaatcgac gatccgcga aaactttta ggctacatt gtctactcg atcgaagca ccaacccag aacgttacg ctctatgat ggacgagat gcttgtgga ggagacgga tggcgcgtt gatgatgtt gcaattcct gaaaacgaa atgaatgtt acacatcca cttacatcg ttaaaaccc tacacacag tacgcattt tatgtcaaa acttacacg atagctact gaaagaaga ggtgcacaa agtatgatt tcgtacttc actacgttg cccgatact ccaacggca ccggttggc ttagaggta gttagtaat tcgagcaac tctcttaag ttatcgtgg aggcctccc aaatctccc aacggcaaa cttactcac tacattgtt agtttgaaa aggcacaac agtactact cccgatctg gatgaaatg gagcatttc tgcaaatca ccctcgagt cgcccaaaa caccccatt cctagtgtg agcgttcct ccagttact tcaacgaca acaagtaac gacacttgc cagtgttta gaaactaaa ccgtcaaca tcatcaatt aatgaagat gtcgaaaaa tcccgaatt gattttgaa gatgaatta cataacgcg gtttatgtg agaaagccg aatttttca gaatctcgc aaaagacgt gacgtggac agtgagcaa ctaaatgcg aaccgggtg tttaatgaa accgatgaa gccggtgct tatatttcg ttcagtact gtagtcacg gggacagaa ttttacatg ccgaatttg cggcactat acggtttat gaaataaac gtccaagcc tgtagagaa aaaaccaac gacaagctt gataccgaa aatccctgc agtagcaaa aatatgaaa acgcatcgg actctgaag aagaaagga gccgatgat ataaaacaa attgaagtc aaaaaccaa agtttggga atggtgtca cttacgtgg aaggaaccg gaggctccg aacggattg atctattgt tacacgata gaatataaa aagttggaa attgaaaat tccaaggct aatgaagag tatattaca cacgcgcgt tttatcaac cagagtagg atttatacc ttgaaagcg ttatcgcct ggaaattac agtgttcga gtttctgca acgacgtca ggtgattac gccaacttt tctccttac gcgtacttt tatatcgag gaacggccg tctaacact tacgtaacc ttgattgtc tgtatgctc attctggtg ataattttg gcactctgt gcgttttgt ttttacaag aagaagaaa gccgacaag gagagtatg cggctgatt ccgtcggtc aatcccgag tatgttcca agtgtgtat gttccggac gaatgggag gtgcctcgc aaaaaaata gaactgatt agggagtta ggacagggg agttttggc atggtatac gagggcatc gcccaagac gtccgcgga aaggcgcaa atcaaatgc gctgtgaag actgttaat gagcacgcc actaatagg gagcgtttg gagtttttg aacgaagct tccgtcatg aaggctttc gatactgct catgtcgtg aggttgttg ggagtggtg tcgcaaggg cagcccacg ttggtgatc atggagctg atggcgaat ggcgacttg aagacctac ctgcgttcc caccgtcca gacgccgaa gtgtatgac cctgccacg gccaagcaa cccccgacg ctgaagcag atcctgcaa atggccata gaaatagcc gacggaatg gcctacctg tcggccaag aagttcgtc caccgcgac ctggcagct agaaattgc atggtagct gaagacttg actgttaaa ataggtgat tttggtatg acgcgtgat atttacgaa acggactac tatcgcaag ggcactaag gggctgctc cccgtgcga tggatggcc cccgagagt ctcaaagac ggtgtgttt actagtaat agtgatgtt tggagttac ggagttgtg ttgtgggag atggcgact ctggcgtcg cagccgtat caaggcttg tcgaacgac caggttttg cgatatgtg attgacgga ggtgtgatg gagcggccg gagaactgt cccgataaa ctgtacact ttgatgaga tactgttgg caacacaaa ccatcagca cgtccttcg tttttaaag ttatgctca ttgttgtta gaggatgcc agcactagt tttgctcag gtgtcgttt tatcatagt gcggctgga attgaggct cgatcgtcg cggccgaca ccgtcgccg tcgcaggat gatccgagt actccgctc cggatagct ggagaccat gatgtcaat ttttctctc aattcggac gactcgaat gacgagttc gaaacagac gctgagact catattagg tttcccagt attcccgtg gagaacaaa gacggaatc accaccgct aacggttac gtgagtgga tgtcccaca aatggtgca gcaacaact cagtgctag ctacatatc acatctcag ttgaaagta tttcctata aagatctga ctattgcag caagagaca taaataagt aacatgact gtttttgta aagtagtta taagtttaa attaccaaa gatattttc ttcatttgc tgaaaggcc tacatatta cagaaaaac agtgttcat tagcaaatc attaacaag tgaggcgta ttcagtgag cctgaaacc ataaagact agtctattg attttccat tctatggga tactctaag attcttgtc tactcaata ttatgtgta aaagagatg aaactaatt gccttatta gttaaagta attataatg tgtagcatc ccataaaga cattctttg gacaattgc caatcaata cattgcagc ctgactctg agttcctgt tatcttatt gatgattgg tagtcacaa ctactttat tgtacctat ttatatttt agttaaatt ttgtactct gtaatataa tgtacatta tttc
