Status
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Gene information
General information
TC010778 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5148802..5151905
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010778
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01732

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Pupal morphology
    antenna orientation bent - Number of animals showing the phenotype: 4

    elytra dorsalnot closed - Number of animals showing the phenotype: 4

    elytra need termsize elongated - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010778 RA
      MFNPEQFSQ FFKPDDGRY NEMENVDAM LNYENNEFM 
      TSDFFDTIR SVENGIQDD FLNSPTDDF NTLSDASSN 
      TDGYVSPRN DISESERSS IFTDESVSP LRTVDAIDD 
      SLFDFLQPT QEKVEVETV PVIINQPKT RTKIIIQNP 
      ANNKSINKQ LINTPIKKI GKKQILKVQ SLTTNGKPV 
      ILPYGLKSI KILNASELK TDRIHVATF PTNTVRRKV 
      EPTRIKEEV ELTTHRPGE LTEVKLTPE EKRLLEKEG 
      IQLPAYHPL TKLEDRELK RIRRKIRNK ISAQDSRKR 
      KKEYVDGLE EKVRRGSEE NRNLMQRVR ELQKKNKTL 
      MAHIHKLQS LIFNSTSSK ATPSTCLMI VMLSVLLVS 
      LPNIRLSQM NNELATEQE QVTISRSLL STKKGEDDG 
      LNMEEFFVF NKDEEGVDN ENNTENEFF KSIEEMESK 
      YENMNFEKD NSDDNFFNS LVRNIKNLF NKDELGFYN 
      KNGFIEPDI DDFDSGTIN PEPPAKKMK LADEHYHQN 
      DENFVPLAK VITTVHAVG ESKDK
    CDS sequence
    • >TC010778 RA
      atgtttaac ccggagcag ttttcccag tttttcaaa 
      cccgacgat gggcggtac aatgaaatg gagaatgtg 
      gatgctatg ctaaattac gaaaacaac gaatttatg 
      acctcggat tttttcgat accattagg tctgtggaa 
      aacgggatt caggatgac ttcctcaac agccccact 
      gatgatttt aacacctta agcgatgca agctccaac 
      actgatgga tacgtttcg cccagaaac gatatctct 
      gaaagcgaa aggagcagc atcttcact gatgaatct 
      gtttcgcct ctgaggact gtggacgct attgacgat 
      tcattgttt gactttttg caaccaact caagagaag 
      gttgaggtg gaaacggtc cccgttatc attaatcaa 
      ccgaaaaca agaacaaaa ataataatc cagaacccc 
      gccaataac aaatctatc aataaacaa ctaataaat 
      acaccaata aaaaaaatt ggaaagaag cagatattg 
      aaagtgcag tcattgaca accaatggc aagccagta 
      atactcccc tatggctta aaaagcatc aagatactt 
      aacgctagt gaattaaaa actgaccga atccacgtt 
      gcaacgttc ccgactaac accgtcaga cgcaaagtt 
      gaaccgacc agaattaaa gaagaggta gagttgacc 
      acacaccgt cctggagaa ctgactgag gtgaagcta 
      acgcctgaa gagaagcgt cttttggag aaagaaggg 
      attcagcta cctgcctac caccccctg accaaattg 
      gaagacagg gaattgaaa cgtatcaga aggaaaatc 
      cgcaacaag atctccgct caagattcc cgcaagcgt 
      aaaaaggag tacgttgac gggctcgaa gagaaggtc 
      agacggggc tcagaagag aacaggaac ttgatgcaa 
      cgtgtcaga gaattacaa aagaaaaac aaaacgctg 
      atggcacac atccacaag ctccagtcg ctgatcttc 
      aactcgaca tcatcgaaa gccacgcct tcgacctgc 
      ctcatgatc gtgatgttg tcggtcctg ttggtctct 
      ctccctaat attcgtcta tcgcagatg aacaacgag 
      ctggcgacg gagcaagag caagtcacc atcagcaga 
      tctcttctg tctactaag aaaggcgaa gacgatggg 
      ttgaacatg gaggagttc ttcgttttc aacaaggat 
      gaggagggt gtcgacaat gaaaacaac accgagaac 
      gagttcttc aagtcgatt gaagaaatg gaaagcaag 
      tacgaaaac atgaacttc gagaaggat aactcggac 
      gataacttc ttcaactcg ctggtacga aacataaaa 
      aatctgttc aacaaagac gagctcgga ttctacaat 
      aagaacggg tttattgaa ccagacatt gacgatttt 
      gattctggg acaataaac ccagaacca cccgccaaa 
      aagatgaaa ctggccgat gagcactac catcaaaat 
      gacgagaat ttcgttccc ctggccaaa gtcatcact 
      actgtgcat gctgtgggc gaaagcaag gataaataa
    mRNA sequence
    • >TC010778 RA
      agagttttc gggggcaag cctgtgcag tgacgtcat 
      taattccac gctggctgt catggcttt cacatcgaa 
      gtgtgcggt gtaacatat tggaaaaga acagtgtgt 
      atatattga aagttttgc aattaagtg tcggcaaag 
      tgcaatttc ggtgatacc taagaatat gcaaaatgt 
      ttaacccgg agcagtttt cccagtttt tcaaacccg 
      acgatgggc ggtacaatg aaatggaga atgtggatg 
      ctatgctaa attacgaaa acaacgaat ttatgacct 
      cggattttt tcgatacca ttaggtctg tggaaaacg 
      ggattcagg atgacttcc tcaacagcc ccactgatg 
      attttaaca ccttaagcg atgcaagct ccaacactg 
      atggatacg tttcgccca gaaacgata tctctgaaa 
      gcgaaagga gcagcatct tcactgatg aatctgttt 
      cgcctctga ggactgtgg acgctattg acgattcat 
      tgtttgact ttttgcaac caactcaag agaaggttg 
      aggtggaaa cggtccccg ttatcatta atcaaccga 
      aaacaagaa caaaaataa taatccaga accccgcca 
      ataacaaat ctatcaata aacaactaa taaatacac 
      caataaaaa aaattggaa agaagcaga tattgaaag 
      tgcagtcat tgacaacca atggcaagc cagtaatac 
      tcccctatg gcttaaaaa gcatcaaga tacttaacg 
      ctagtgaat taaaaactg accgaatcc acgttgcaa 
      cgttcccga ctaacaccg tcagacgca aagttgaac 
      cgaccagaa ttaaagaag aggtagagt tgaccacac 
      accgtcctg gagaactga ctgaggtga agctaacgc 
      ctgaagaga agcgtcttt tggagaaag aagggattc 
      agctacctg cctaccacc ccctgacca aattggaag 
      acagggaat tgaaacgta tcagaagga aaatccgca 
      acaagatct ccgctcaag attcccgca agcgtaaaa 
      aggagtacg ttgacgggc tcgaagaga aggtcagac 
      ggggctcag aagagaaca ggaacttga tgcaacgtg 
      tcagagaat tacaaaaga aaaacaaaa cgctgatgg 
      cacacatcc acaagctcc agtcgctga tcttcaact 
      cgacatcat cgaaagcca cgccttcga cctgcctca 
      tgatcgtga tgttgtcgg tcctgttgg tctctctcc 
      ctaatattc gtctatcgc agatgaaca acgagctgg 
      cgacggagc aagagcaag tcaccatca gcagatctc 
      ttctgtcta ctaagaaag gcgaagacg atgggttga 
      acatggagg agttcttcg ttttcaaca aggatgagg 
      agggtgtcg acaatgaaa acaacaccg agaacgagt 
      tcttcaagt cgattgaag aaatggaaa gcaagtacg 
      aaaacatga acttcgaga aggataact cggacgata 
      acttcttca actcgctgg tacgaaaca taaaaaatc 
      tgttcaaca aagacgagc tcggattct acaataaga 
      acgggttta ttgaaccag acattgacg attttgatt 
      ctgggacaa taaacccag aaccacccg ccaaaaaga 
      tgaaactgg ccgatgagc actaccatc aaaatgacg 
      agaatttcg ttcccctgg ccaaagtca tcactactg 
      tgcatgctg tgggcgaaa gcaaggata aataaaatt 
      tgtcttctg tgcttctta tatattctt ataatagtt 
      tggttcttt tacactttg cagtttgtg tcttttaaa 
      atgatttca ccgagtcat atagcttga agtttttag 
      ttttatagc gtgatagtt ccttgtcct tttttatct 
      atgtgttac ttggtaaga aagttattt cataatgga 
      aagagtttg cattccaat atattgatt acccgaaag 
      ttatgtata ttttttcaa gtctttggc agagttcag 
      attgaaaat agactgtga atttaacta tgagattgt 
      tattgtatt tattcagtt gtaagtttt tttcagaca 
      gatttatac aaatagggt tcttagtca aatcctgtt 
      gtggacgtt attaaaatg atttatgaa atgtgtgta 
      ttgagcggc ttaaataca accattgag tttcttaaa 
      tatatatta tatgtacta aactgaatg ataactaag 
      cctaaaata tgttttgtt gcgcagtta cactgcttt 
      tcgaaattc tgttaactc taagaaaat tacagtggt 
      attgtacct atccttgtt aaaatctaa cataaatta 
      ttttgtacg tacttgtaa aaaataaat atcttttag 
      ttatgtaat tcagttaaa ataaatatt tctaataat 
      t
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