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Gene information
General information
TC010774 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5061236..5064795
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010774
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01731

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010774 RA
      MSTQVFILS GCRTAIGSF QGQFDKIPA SELGSVVIT 
      EALKRATIS PQDVDQVIM GQILTAGQG QNPARQASV 
      GAQVPYAVP AYTINMLCG SGLKSVCLA YQAIRNQDN 
      EIVVAGGQE NMSRAQHCA YIRGNKLGA VQMNDTLLY 
      DGLTDAFNS IHMGKTAEH LAKEYKITR EAQDNFALT 
      SQTKTQEAI QQGYFKKEI IGVVEKRTG KVLDKDEFP 
      KAGTTLDGL AKLRPAFDP NGTVTAGNA SGINDGAAA 
      LVLCSENQV KLKGLKPLA RIVACAEVG IDPLCMGLG 
      PIEAVNKVL LKTGWSRHD VDLYELNEA FAVQSIIVG 
      EALQVNAKK VNITGGAIA LGHPIGASG ARILVTLIH 
      NLERLNMKK GVAALCIGG GMGIAIAIE MC
    CDS sequence
    • >TC010774 RA
      atgtcgact caagtattt attttgtca ggctgtagg 
      accgcaatt gggagtttc cagggccag tttgataaa 
      atccccgcg agcgagttg ggttcagtt gtaataaca 
      gaggcctta aaacgtgcg acaataagc ccccaagat 
      gtggaccaa gtgataatg ggtcaaatt ttgactgca 
      ggtcaaggt cagaaccca gcacgccag gcttcggtc 
      ggggcccaa gtaccgtac gctgttccc gcatacact 
      attaatatg ctatgcggc tcaggcttg aaatccgtg 
      tgccttgcg tatcaggcg attcgtaat caggacaat 
      gagattgtg gtggccggg ggccaggag aacatgtca 
      cgggcccaa cactgcgcg tacattcgc gggaataaa 
      ctgggggct gtccaaatg aatgacacc ctgctgtat 
      gacggtctc accgacgcc tttaacagt attcatatg 
      ggaaaaacc gctgaacat ttagcgaaa gagtataaa 
      attacgcgc gaggcgcaa gataacttc gccctgacg 
      tcacaaaca aagacgcag gaggcgatc caacaagga 
      tattttaaa aaggaaatc atcggagtt gtggagaag 
      agaactggg aaagtttta gataaagac gaattcccc 
      aaagctggg acgactctc gacggtttg gcgaagcta 
      aggccagcc tttgacccc aatgggacc gtaactgcc 
      ggaaacgca tccgggatt aatgatggg gcggctgcc 
      cttgtttta tgtagcgaa aatcaggtg aaattgaag 
      ggtttaaag cctctggct agaattgtg gcttgtgcc 
      gaggtcggg attgacccc ctgtgcatg gggcttggg 
      cccattgag gctgtgaac aaagtcctg cttaagacg 
      ggttggtca agacacgac gtcgatttg tacgaactg 
      aacgaagct tttgccgtt cagagcatc attgtgggc 
      gaagcgctc caagtcaat gcgaaaaaa gtcaacata 
      acgggaggt gctatcgct ttagggcac ccgatcggg 
      gcttcgggg gcccgcatc ctcgtcact ctcatccat 
      aatttggaa cgtttgaat atgaaaaaa ggagtggca 
      gctttgtgt attggagga ggcatgggt atagccatc 
      gcgattgaa atgtgttaa
    mRNA sequence
    • >TC010774 RA
      aaacaatta attgttcaa ctacgacac tgtcccttt 
      ttaattgtt cctttcaat ggttaaaaa agccgcatt 
      atggtcaaa agaaaaaag gggagggta cccgtttca 
      cggtgtaaa acaaataaa catgatgaa aatcctcgt 
      taagtgtgt tgcacccta acctaagtt tttgttccg 
      gtccggttc tcgtgaaaa tgtgcagtt gaaaagcgg 
      ccacacact tagacagct ttctcttcc atcactcat 
      caacaagga gtattagtt ttcattcac agtattcaa 
      gaatgtcga ctcaagtat ttattttgt caggctgta 
      ggaccgcaa ttgggagtt tccagggcc agtttgata 
      aaatccccg cgagcgagt tgggttcag ttgtaataa 
      cagaggcct taaaacgtg cgacaataa gcccccaag 
      atgtggacc aagtgataa tgggtcaaa ttttgactg 
      caggtcaag gtcagaacc cagcacgcc aggcttcgg 
      tcggggccc aagtaccgt acgctgttc ccgcataca 
      ctattaata tgctatgcg gctcaggct tgaaatccg 
      tgtgccttg cgtatcagg cgattcgta atcaggaca 
      atgagattg tggtggccg ggggccagg agaacatgt 
      cacgggccc aacactgcg cgtacattc gcgggaata 
      aactggggg ctgtccaaa tgaatgaca ccctgctgt 
      atgacggtc tcaccgacg cctttaaca gtattcata 
      tgggaaaaa ccgctgaac atttagcga aagagtata 
      aaattacgc gcgaggcgc aagataact tcgccctga 
      cgtcacaaa caaagacgc aggaggcga tccaacaag 
      gatatttta aaaaggaaa tcatcggag ttgtggaga 
      agagaactg ggaaagttt tagataaag acgaattcc 
      ccaaagctg ggacgactc tcgacggtt tggcgaagc 
      taaggccag cctttgacc ccaatggga ccgtaactg 
      ccggaaacg catccggga ttaatgatg gggcggctg 
      cccttgttt tatgtagcg aaaatcagg tgaaattga 
      agggtttaa agcctctgg ctagaattg tggcttgtg 
      ccgaggtcg ggattgacc ccctgtgca tggggcttg 
      ggcccattg aggctgtga acaaagtcc tgcttaaga 
      cgggttggt caagacacg acgtcgatt tgtacgaac 
      tgaacgaag cttttgccg ttcagagca tcattgtgg 
      gcgaagcgc tccaagtca atgcgaaaa aagtcaaca 
      taacgggag gtgctatcg ctttagggc acccgatcg 
      gggcttcgg gggcccgca tcctcgtca ctctcatcc 
      ataatttgg aacgtttga atatgaaaa aaggagtgg 
      cagctttgt gtattggag gaggcatgg gtatagcca 
      tcgcgattg aaatgtgtt aattgtttt tattggttt 
      tgaataaag gaatttgca aaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY