Status
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Gene information
General information
TC010772 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4992926..4996899
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010772
    Gene ontology

    GO terms for Tribolium

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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01730

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010772 RA
      MPIQEIPRV PETINFPSE EEKILKYWT EIDVFQTCL 
      KQSKGKPRF SFYDGPPFA TGLPHYGHI LAGTIKDVV 
      TRYAHQQGF HVERRFGWD CHGLPVEYE IDKTFDIKG 
      PDDVMKMGI DKYNAECRK IVMRYADEW ERIIGRIGR 
      WIDFKNDYK TLYPTFMES VWWVFKQLY LKGLVYQGN 
      KVMPYSTTC NTPLSNFES GQNYKDVVD PAVTVSLPI 
      IGHKDNAAL LVWTTTPWT LPSNLAACV NPSLEYVLF 
      EQISTTKRY IMLETRIET IFPAGDYKI IRKFPGSEL 
      KGLRYEPIF PYFAHLKDK AFRVLTDDY VTSESGTGV 
      VHQAPYFGE DDFRVCLNA GVITKDQEP ICPVDASGR 
      FVKPVVEFE GLYVKDADK KIIAALKTN GRLVHQTQI 
      KHSYPFCWR SDTPLIYKA VPSWFVRVE HMTEELQKA 
      CADTYWVPD FVKDKRFGN WLRDARDWA ISRNRYFGT 
      PIPIWASPS GNEFVCVGS IAELEQLTG QKITDLHRE 
      SIDHLEIPS KIPGNPPLR RVPEVFDCW FESGSMPYA 
      QKHYPFEFS KEFDDYFPA DFIAEGIDQ TRGWFYTLL 
      VISTALFGK APYKNLIAN GLVLASDGQ KMSKRKKNY 
      PDPLEIVSK YGADALRLY LINSPVVRA ENLRFKEEG 
      VRDILKDVF LPWYNAFRF LLQNIESFV QDNNQAFYY 
      TENSVAPEN IMDKWILSF TQSLLEYVR KEMQLYHLY 
      NVIPRLTKF IDYLTNWYV RMNRKRLKG EGGFDDCKT 
      ALLTLFEVL LNIVKMMAP FSPFLAETM YQYLKKLMK 
      TSADSVHYL MLPQPNHAL INKDIERAV ARMQSVIEL 
      GRVLRDRKT IPIKYPLPE VIIVHQDPQ YISDILSLK 
      EYIHLELNV RAINTTTDK SKYGITLRA EPDYKVLGQ 
      RLKQELKAV TAAIQALTD VEINEMVKA GFRVVAGQR 
      IEISEVRLI FKAESINTS QYEVNSDND VLILLDITP 
      DSSMQDEGT AREIINRIQ KLRKKAHLV PTDQVSVFY 
      KTTGELDRV AKSYCDFVE NTIKATFKP IEQQQNSDL 
      VIIEEVQQL KNCNLQLVL TRFSDIVIP ASKWVNIQL 
      VGLESRYCN QSPKALALL EVANKPVSL DNLHSSVFR 
      LFGVEHYNL VKSNGKEVT QEELPNLSG QTIFVIPTN 
      KDVKLPEMS RVPFCKVFN FVAQNTSGT LILENPAGN 
      ETLNEKDFK EIINQWIKL A
    CDS sequence
    • >TC010772 RA
      atgcctatt caagaaatt ccccgtgtg ccggaaacc 
      ataaatttc ccatccgag gaagaaaaa atcctgaaa 
      tattggacg gaaatcgac gtgttccaa acatgcttg 
      aaacagtcg aagggaaag ccacggttt tcgttctat 
      gacggcccg ccgtttgcg accgggttg ccccattat 
      gggcacatt ctggcgggg accatcaaa gacgtggtc 
      acgcgttat gcccaccag cagggcttc cacgtggag 
      aggaggttc ggctgggac tgccatggg ctacccgtg 
      gagtacgag attgacaag acttttgat attaagggc 
      ccggatgat gtaatgaag atgggcatt gacaaatac 
      aacgccgag tgccgcaag attgtcatg cggtacgcc 
      gacgagtgg gagaggatc attgggcgc attggacgc 
      tggattgat tttaaaaac gactacaag acgctttac 
      ccgactttc atggagtcg gtttggtgg gttttcaag 
      caattgtac ttaaaggga ttagtttat cagggcaat 
      aaagtaatg ccgtactcg acgacgtgt aataccccc 
      ttatcgaac ttcgagtcg ggtcagaat tacaaggat 
      gttgttgat cccgccgtc accgtgagc ctcccgatc 
      atcggtcat aaggacaat gctgcgctt ttagtttgg 
      acaacgact ccttggacc ctccctagt aacctagcg 
      gcgtgcgta aacccatcg cttgaatac gtactgttt 
      gaacaaatc agcaccacc aagcgctac atcatgcta 
      gagactcgc attgaaaca attttccca gctggggat 
      tataaaata atccggaaa ttccccgga agcgaattg 
      aaggggctg cgctacgaa ccaattttc ccctacttc 
      gcccacttg aaagacaaa gctttccgg gtcttgaca 
      gacgactac gtcacgtct gagtcgggt acgggtgtc 
      gtgcaccag gcaccgtac ttcggggaa gacgatttc 
      cgcgtctgt ctcaacgct ggggttata accaaggac 
      caggaaccc atctgtccc gttgacgcc agcggccgg 
      ttcgtcaaa cctgtggtg gagttcgaa gggctttat 
      gtcaaagac gccgacaag aaaatcatc gcggctttg 
      aaaaccaac ggccggctc gtgcaccaa actcaaatc 
      aagcacagt taccccttc tgttggcgt tccgacacc 
      cccttgatc tacaaagcg gtgccgtcg tggttcgtc 
      cgcgtggaa cacatgacc gaggagctg caaaaggcc 
      tgtgcggac acctactgg gtgcccgat ttcgtcaaa 
      gacaagcgt ttcggtaac tggctgcgc gacgcccgg 
      gactgggct atcagtcgc aaccggtat ttcggcacc 
      ccgataccg atctgggcg tcgccaagc ggcaatgag 
      ttcgtctgc gttggcagt atcgccgaa ctcgagcaa 
      ctaacgggt cagaaaatc accgatttg catcgggaa 
      agcatcgat catcttgaa attccctcg aaaattcct 
      ggaaatccc ccgttgaga cgcgttcct gaagttttt 
      gattgttgg ttcgagtcg ggctctatg ccctacgcc 
      cagaaacac tacccgttt gagtttagt aaagagttt 
      gatgactat ttcccggcg gattttatc gccgaaggg 
      atcgaccag accaggggc tggttttat acgctttta 
      gtcatctcg acggctttg ttcgggaaa gcgccgtac 
      aagaatttg atcgcgaac gggcttgtg ttggccagc 
      gatgggcaa aagatgtcg aagcggaaa aagaattat 
      ccggacccg ttggaaatc gtttcgaag tatggggcg 
      gacgctttg aggctttat ttgattaac tcgccagtg 
      gtgcgtgcc gaaaacttg cggtttaag gaggaaggg 
      gttagggat attcttaaa gatgtgttc ctgccgtgg 
      tacaatgcg ttccgtttc ttgttacaa aatattgag 
      agttttgta caagacaac aatcaagcg ttctactac 
      actgaaaat agtgtcgcg cctgagaat atcatggac 
      aagtggatt ctttcgttc actcagtcg ctcctagag 
      tacgtccgg aaggagatg caactttac cacttgtac 
      aacgttatt cccaggctg accaagttt attgactac 
      ttaaccaat tggtacgtg cggatgaac cggaaacgt 
      ctcaagggt gagggcggg ttcgacgat tgtaaaaca 
      gccctactg acgctgttc gaggttctc ctaaacatt 
      gtgaaaatg atggcaccg ttctcaccc ttcttggcc 
      gagaccatg taccaatat ttgaagaag ttgatgaag 
      acttcggcc gatagtgtt cactatttg atgttaccg 
      cagccgaat catgcgttg atcaacaaa gatattgag 
      cgagccgtt gccagaatg caaagtgtg atagaattg 
      gggcgagtc ttgcgcgac cgaaaaaca ataccgatc 
      aagtacccg ttgccggaa gttataatt gtccatcag 
      gaccctcag tacatttct gatatcctc tcgttgaag 
      gaatacatc catctcgag ttaaacgtg cgtgcaatc 
      aacacaacg actgataaa agcaaatac gggataacg 
      ctgagggcg gagcccgat tataaagtc ctagggcag 
      cggcttaag caagaactg aaggctgtg actgcagca 
      atacaggcg ctgaccgat gttgagata aatgaaatg 
      gttaaagcc gggttccgg gtcgtggcg ggccaacgt 
      atcgaaatt tccgaagtt aggctcatc ttcaaagct 
      gagagtatt aatacaagt cagtatgaa gtcaacagc 
      gacaacgac gttttgatt ctcttggac ataacgccg 
      gacagttca atgcaagat gaaggcacg gcgagggag 
      ataataaat cgtatccaa aagttgcgc aagaaggca 
      catctggtg ccaacagac caagtttca gtattttac 
      aaaacgact ggtgaacta gatcgcgtc gctaaatcc 
      tattgcgat ttcgtagag aatacaatc aaagcaacg 
      ttcaagcca atcgagcag cagcaaaat tccgacttg 
      gtgataatt gaggaagtg caacaactg aaaaactgt 
      aatctacaa ctggtcctg acccggttt tccgatatt 
      gtaatcccg gctagtaaa tgggttaat atccagctt 
      gtaggcctc gagtccaga tactgtaac caatctcct 
      aaagcttta gctttgctt gaagtggct aataagccg 
      gtcagttta gataatttg cattcgtcg gttttccga 
      ctctttggc gtggaacat tacaatcta gtgaaaagc 
      aacggcaag gaagtaacc caagaggaa ttacctaac 
      ttgtcggga caaacaatt tttgtaatt ccgacaaat 
      aaagatgtt aaattgccc gaaatgagc agagtacct 
      ttctgcaaa gtttttaat tttgttgca caaaatacc 
      agtggcacg ttgatcttg gaaaacccc gcagggaac 
      gaaacttta aatgaaaaa gacttcaag gaaatcata 
      aaccagtgg attaaattg gcttaa
    mRNA sequence
    • >TC010772 RA
      tgttttcgt tttccccca agccagttg gcagtgaat 
      ttcatcatc cgaaaaatt gaggttgtg tcgttgcac 
      gagcataat atgtgatat aatttattg aattagtga 
      aatagctcg caccatgcc tattcaaga aattccccg 
      tgtgccgga aaccataaa tttcccatc cgaggaaga 
      aaaaatcct gaaatattg gacggaaat cgacgtgtt 
      ccaaacatg cttgaaaca gtcgaaggg aaagccacg 
      gttttcgtt ctatgacgg cccgccgtt tgcgaccgg 
      gttgcccca ttatgggca cattctggc ggggaccat 
      caaagacgt ggtcacgcg ttatgccca ccagcaggg 
      cttccacgt ggagaggag gttcggctg ggactgcca 
      tgggctacc cgtggagta cgagattga caagacttt 
      tgatattaa gggcccgga tgatgtaat gaagatggg 
      cattgacaa atacaacgc cgagtgccg caagattgt 
      catgcggta cgccgacga gtgggagag gatcattgg 
      gcgcattgg acgctggat tgattttaa aaacgacta 
      caagacgct ttacccgac tttcatgga gtcggtttg 
      gtgggtttt caagcaatt gtacttaaa gggattagt 
      ttatcaggg caataaagt aatgccgta ctcgacgac 
      gtgtaatac ccccttatc gaacttcga gtcgggtca 
      gaattacaa ggatgttgt tgatcccgc cgtcaccgt 
      gagcctccc gatcatcgg tcataagga caatgctgc 
      gcttttagt ttggacaac gactccttg gaccctccc 
      tagtaacct agcggcgtg cgtaaaccc atcgcttga 
      atacgtact gtttgaaca aatcagcac caccaagcg 
      ctacatcat gctagagac tcgcattga aacaatttt 
      cccagctgg ggattataa aataatccg gaaattccc 
      cggaagcga attgaaggg gctgcgcta cgaaccaat 
      tttccccta cttcgccca cttgaaaga caaagcttt 
      ccgggtctt gacagacga ctacgtcac gtctgagtc 
      gggtacggg tgtcgtgca ccaggcacc gtacttcgg 
      ggaagacga tttccgcgt ctgtctcaa cgctggggt 
      tataaccaa ggaccagga acccatctg tcccgttga 
      cgccagcgg ccggttcgt caaacctgt ggtggagtt 
      cgaagggct ttatgtcaa agacgccga caagaaaat 
      catcgcggc tttgaaaac caacggccg gctcgtgca 
      ccaaactca aatcaagca cagttaccc cttctgttg 
      gcgttccga caccccctt gatctacaa agcggtgcc 
      gtcgtggtt cgtccgcgt ggaacacat gaccgagga 
      gctgcaaaa ggcctgtgc ggacaccta ctgggtgcc 
      cgatttcgt caaagacaa gcgtttcgg taactggct 
      gcgcgacgc ccgggactg ggctatcag tcgcaaccg 
      gtatttcgg caccccgat accgatctg ggcgtcgcc 
      aagcggcaa tgagttcgt ctgcgttgg cagtatcgc 
      cgaactcga gcaactaac gggtcagaa aatcaccga 
      tttgcatcg ggaaagcat cgatcatct tgaaattcc 
      ctcgaaaat tcctggaaa tcccccgtt gagacgcgt 
      tcctgaagt ttttgattg ttggttcga gtcgggctc 
      tatgcccta cgcccagaa acactaccc gtttgagtt 
      tagtaaaga gtttgatga ctatttccc ggcggattt 
      tatcgccga agggatcga ccagaccag gggctggtt 
      ttatacgct tttagtcat ctcgacggc tttgttcgg 
      gaaagcgcc gtacaagaa tttgatcgc gaacgggct 
      tgtgttggc cagcgatgg gcaaaagat gtcgaagcg 
      gaaaaagaa ttatccgga cccgttgga aatcgtttc 
      gaagtatgg ggcggacgc tttgaggct ttatttgat 
      taactcgcc agtggtgcg tgccgaaaa cttgcggtt 
      taaggagga aggggttag ggatattct taaagatgt 
      gttcctgcc gtggtacaa tgcgttccg tttcttgtt 
      acaaaatat tgagagttt tgtacaaga caacaatca 
      agcgttcta ctacactga aaatagtgt cgcgcctga 
      gaatatcat ggacaagtg gattctttc gttcactca 
      gtcgctcct agagtacgt ccggaagga gatgcaact 
      ttaccactt gtacaacgt tattcccag gctgaccaa 
      gtttattga ctacttaac caattggta cgtgcggat 
      gaaccggaa acgtctcaa gggtgaggg cgggttcga 
      cgattgtaa aacagccct actgacgct gttcgaggt 
      tctcctaaa cattgtgaa aatgatggc accgttctc 
      acccttctt ggccgagac catgtacca atatttgaa 
      gaagttgat gaagacttc ggccgatag tgttcacta 
      tttgatgtt accgcagcc gaatcatgc gttgatcaa 
      caaagatat tgagcgagc cgttgccag aatgcaaag 
      tgtgataga attggggcg agtcttgcg cgaccgaaa 
      aacaatacc gatcaagta cccgttgcc ggaagttat 
      aattgtcca tcaggaccc tcagtacat ttctgatat 
      cctctcgtt gaaggaata catccatct cgagttaaa 
      cgtgcgtgc aatcaacac aacgactga taaaagcaa 
      atacgggat aacgctgag ggcggagcc cgattataa 
      agtcctagg gcagcggct taagcaaga actgaaggc 
      tgtgactgc agcaataca ggcgctgac cgatgttga 
      gataaatga aatggttaa agccgggtt ccgggtcgt 
      ggcgggcca acgtatcga aatttccga agttaggct 
      catcttcaa agctgagag tattaatac aagtcagta 
      tgaagtcaa cagcgacaa cgacgtttt gattctctt 
      ggacataac gccggacag ttcaatgca agatgaagg 
      cacggcgag ggagataat aaatcgtat ccaaaagtt 
      gcgcaagaa ggcacatct ggtgccaac agaccaagt 
      ttcagtatt ttacaaaac gactggtga actagatcg 
      cgtcgctaa atcctattg cgatttcgt agagaatac 
      aatcaaagc aacgttcaa gccaatcga gcagcagca 
      aaattccga cttggtgat aattgagga agtgcaaca 
      actgaaaaa ctgtaatct acaactggt cctgacccg 
      gttttccga tattgtaat cccggctag taaatgggt 
      taatatcca gcttgtagg cctcgagtc cagatactg 
      taaccaatc tcctaaagc tttagcttt gcttgaagt 
      ggctaataa gccggtcag tttagataa tttgcattc 
      gtcggtttt ccgactctt tggcgtgga acattacaa 
      tctagtgaa aagcaacgg caaggaagt aacccaaga 
      ggaattacc taacttgtc gggacaaac aatttttgt 
      aattccgac aaataaaga tgttaaatt gcccgaaat 
      gagcagagt acctttctg caaagtttt taattttgt 
      tgcacaaaa taccagtgg cacgttgat cttggaaaa 
      ccccgcagg gaacgaaac tttaaatga aaaagactt 
      caaggaaat cataaacca gtggattaa attggctta 
      aggagagtt tagtttctt tttgaatat tgttaagtt 
      tttgggtgt ttatattgt atttttccg ttccgaagt 
      caataaaaa attgtttga taa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY