Status
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Gene information
General information
TC010766 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4943166..4952973
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010766
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01728

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: >5 - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010766 RA
      MSVCLESSR PRDGLKLPL NRSFSEPGP PTPGHLTLS 
PPKRCKLDT PSVSLPTSP CAESATLQR ALVLKKVKT 
LDPDGLKVK LEAARHGPK SFIVLDCRP FISYNVNHI 
NGAININCS DRFNRRRLQ QGKATLADL ATTREGKDV 
LKRRAFKEV IVYDDNTCD KERITTAQP LLLVLSSLV 
EDNKEPALL IGGHTEFHR KHKDLCEDT LVTGLSSRG 
LPSSASCPA LADIDSHPA SRVLPFLYL GNSKNAADL 
SCLQDLGTT CVLNVTPQL AGYHETCGI TYKQIPATD 
NGHQNLKQY FEEAFEFIE EARNNGLRV LVHCQAGIS 
RSATIAIAY IMKYKQMSM VEAYKLVKA CRPIISPNL 
NFMGQLLEL EQSLRSGSN NNNHQCRWS QQSSDEVTQ 
GCSV
    CDS sequence
    • >TC010766 RA
      atgtcagtg tgtctcgaa agcagtcgg ccgcgggac 
ggtctaaag cttcctcta aaccgaagt tttagtgag 
cctggaccc ccgaccccg ggccatctc acgctaagt 
ccccccaag cgctgcaag ctggatacc ccgagcgtg 
agcctgccg acgagtccg tgcgccgag agcgcgacc 
ctccagagg gccctcgtc ctgaagaag gtcaagacg 
ctggacccc gacgggctc aaagtcaag ctcgaggcg 
gcccggcat ggccccaag tccttcatc gtgctggac 
tgccgcccg ttcatctcc tacaacgtc aaccacatc 
aacggggcc atcaacatc aactgctcg gacaggttc 
aaccggcgc cggctgcag caggggaag gccaccctg 
gccgacctg gccaccacg agggaggga aaagacgtg 
ctcaagcgg agggccttc aaggaggtc atcgtctac 
gacgacaac acgtgtgat aaagagcgg attacgaca 
gcgcagccc cttcttctc gtgctttcc tccctcgtc 
gaagacaac aaggagccg gcgctcctc atcggaggt 
cacaccgaa ttccatcgt aaacacaag gatttgtgc 
gaagacact ctggtcacc ggcctatca tctcggggc 
ctgccctcg tcggcctcc tgtccggcc ctcgccgac 
atagactcg catcctgcc tctcgagtg ctgcccttc 
ttgtatctg ggcaacagc aagaatgca gctgacttg 
tcctgtcta caagatctg ggcaccacc tgcgttctt 
aatgttaca ccacagctg gcggggtac cacgaaacc 
tgcgggatc acgtacaag caaatacct gccaccgac 
aacggacat caaaacctc aaacagtat tttgaagag 
gctttcgaa ttcatcgag gaagcgcgg aacaacggg 
ctgcgggtg ttggtgcat tgccaggcc ggaatttcc 
cgcagcgcc acaatagcg atcgcttat ataatgaaa 
tataaacaa atgtcgatg gttgaggcg tacaaactg 
gtgaaagct tgccggccg ataatatcc ccgaatttg 
aatttcatg ggccaactg ttggagcta gaacaaagt 
ttacgtagt ggtagcaat aacaacaat catcagtgt 
cgctggagc cagcagtcc agtgacgaa gtgacacaa 
ggctgtagt gtttga
    mRNA sequence
    • >TC010766 RA
      catccgtca tcgacgact atacgcata cgaggttat 
tttagttag tatcgcaaa tttcgcgcc tttaaggat 
gttccatat tgaacgtgt ctatcattt aatcggcga 
acgctagtg gaaatttct gtgccacga tatgttaat 
gttggtgaa atgtgttcc tatttgtaa ataatgttt 
taaacacct tgtgtcaaa acagtcgga tttaacggc 
ctttgcggc caacttcga tatagcgcg tgacgtgcg 
taactagcg tcatgtcag tgtgtctcg aaagcagtc 
ggccgcggg acggtctaa agcttcctc taaaccgaa 
gttttagtg agcctggac ccccgaccc cgggccatc 
tcacgctaa gtcccccca agcgctgca agctggata 
ccccgagcg tgagcctgc cgacgagtc cgtgcgccg 
agagcgcga ccctccaga gggccctcg tcctgaaga 
aggtcaaga cgctggacc ccgacgggc tcaaagtca 
agctcgagg cggcccggc atggcccca agtccttca 
tcgtgctgg actgccgcc cgttcatct cctacaacg 
tcaaccaca tcaacgggg ccatcaaca tcaactgct 
cggacaggt tcaaccggc gccggctgc agcagggga 
aggccaccc tggccgacc tggccacca cgagggagg 
gaaaagacg tgctcaagc ggagggcct tcaaggagg 
tcatcgtct acgacgaca acacgtgtg ataaagagc 
ggattacga cagcgcagc cccttcttc tcgtgcttt 
cctccctcg tcgaagaca acaaggagc cggcgctcc 
tcatcggag gtcacaccg aattccatc gtaaacaca 
aggatttgt gcgaagaca ctctggtca ccggcctat 
catctcggg gcctgccct cgtcggcct cctgtccgg 
ccctcgccg acatagact cgcatcctg cctctcgag 
tgctgccct tcttgtatc tgggcaaca gcaagaatg 
cagctgact tgtcctgtc tacaagatc tgggcacca 
cctgcgttc ttaatgtta caccacagc tggcggggt 
accacgaaa cctgcggga tcacgtaca agcaaatac 
ctgccaccg acaacggac atcaaaacc tcaaacagt 
attttgaag aggctttcg aattcatcg aggaagcgc 
ggaacaacg ggctgcggg tgttggtgc attgccagg 
ccggaattt cccgcagcg ccacaatag cgatcgctt 
atataatga aatataaac aaatgtcga tggttgagg 
cgtacaaac tggtgaaag cttgccggc cgataatat 
ccccgaatt tgaatttca tgggccaac tgttggagc 
tagaacaaa gtttacgta gtggtagca ataacaaca 
atcatcagt gtcgctgga gccagcagt ccagtgacg 
aagtgacac aaggctgta gtgtttgaa tgcgttatg 
aactctagg ggtttggag tggagcctc acattaaat 
aaattttag tcattcaac tcgccattt aatttgcat 
tctagtcta tgattggtg aagcattga ttttgggaa 
gagagagct ttaatagtc gcggattta tcgcagcct 
ttgcaaatt tgggtagct ttaaattac taatttaac 
gacatgatc aatgcttta ctgtttgag aaaatggct 
gtgtaccaa taaagatat tcactataa gatagtatg 
cagtagaaa ttatgattt tgcgtattt tttacttcc 
aagcagctg gtttcaagt gtggctagt ggtcgagga 
attagtcgt ggaatgaat tcagtgatt ttgaagtag 
gttgagtca agtcgtgtt gtcgcaaaa tgaagtaat 
ttcataagc agacatcgg ttcttagtg tattttatt 
tattactta tatgaattt atatatcag aaacctaac 
tatgtaaag ttctacggg ttcgagtcc cactattat 
attatgtca taaaacgtt gccatagtt tatcatgaa 
aataagtca gtgttttag ttttagtta gtaatgatt 
aatcgtatg ttttttatg ttgatctat atttaaagg 
acttttgtt tggaatatt actttagtt tcgattaat 
aaggaagta ttttttatg actagagaa tcaacatat 
ttattgaaa ttgtcgttg tgatttttc tcatagtat 
cttaagcct tggcgtgaa cgattaggt agaaaaagg 
tgcgaaatg atgggatgg tgaactacc aggtcttta 
attacctat taggtgcac actgtatat ttagcgaca 
ccatcgcat taacaaaat tgtgccttc actattgtt 
ttgtaagga acttattta taaaaattc agactgtcc 
acatagttg ttttgttca agtccataa cttatataa 
ttacaaaaa atcctaaaa aaattgtgt ttc

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY