Status
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Gene information
General information
TC010761 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4844967..4851923
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010761
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01727

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010761 RA
      MAQDDLINI DSMLSHAGD FGRHQCILM FLFSVINVL 
      SAFHYFGQI FISIVPEHK CRMSTKEDP RFIAAEQCS 
      YTIFHEDIY QKIPCSEDW DYNNSYGYV SIVQELDWV 
      CDESWRPAL GQSVFFIGS VIGSLFLGI LADSIGRLH 
      VLVASNLLA FFGNITTLI TTNTTIFAM CRFLAGCAT 
      DANFVMMYI IVMEYIRPS MRTFGLNLC IGVFYCFAC 
      MVIPWVAVW LQNWESLLL AIAIPHLSV LSFYFIVPE 
      SAQWLISKG RTEEAIDCF KRIARVNGK KLSDKHFEG 
      LTRYCCQHI NARKKHQSF LSLLKTPKL RRKTLILIF 
      KSMVMTLCY DAISRNIDN VGYSPFLVF TITSATILP 
      ACLCILAVQ DRIGRKALA SGSLLMSGI FTACSGIVQ 
      VFIETPHPT LIVVLAIIA RLAVTVAYN SGAQYAVEL 
      IPTVVRAQG VSIIHVTGY AATFFSPQI LYLARFWKP 
      SPEVILGVL LVLGAGACL FLPETLNRT LPVTLQDGE 
      EFGEDENVF DFACCRAPT ESTTQLTRD QIHTNYM
    CDS sequence
    • >TC010761 RA
      atggctcaa gatgactta attaatatt gacagcatg 
      ctctcgcat gccggggat ttcggacga caccagtgc 
      atcctcatg tttttgttc agtgtaata aatgtgctg 
      tctgccttt cattacttc ggccagatt tttatcagt 
      attgtcccc gaacacaag tgcagaatg tccacaaag 
      gaagatccg cggtttatt gccgcggaa caatgttcc 
      tacacgatt tttcacgaa gacatttac caaaaaata 
      ccctgctct gaagactgg gactacaac aacagttac 
      ggctacgtc agtattgtt caagagctc gattgggtg 
      tgtgacgag agctggagg cccgctctt ggacaatca 
      gtgtttttt atcggatcg gtcatcggg agcctattt 
      ctcggaatc ctggccgat agtatcggc agactacac 
      gtcttggtg gcgtcaaat ttacttgcc tttttcgga 
      aacatcacg acacttatc acgacaaac actacgatt 
      tttgcaatg tgtcgcttc ttggccgga tgtgcaacc 
      gacgctaat ttcgtcatg atgtacatt atagttatg 
      gaatacatt cggcccagt atgcgcact tttggccta 
      aacctttgc attggagtt ttctactgt ttcgcttgc 
      atggtaata ccatgggtg gccgtttgg ttgcaaaat 
      tgggaatcc cttcttctg gcgattgca attccgcac 
      ttatcagtt ctgagcttc tacttcata gtaccagaa 
      agtgcccag tggttgata agcaaagga cgcaccgaa 
      gaagctatt gattgtttc aagcgcatt gctcgagtt 
      aatgggaaa aaattaagt gataaacat ttcgaaggc 
      ctgactcgt tactgctgc caacacata aatgcgaga 
      aaaaagcac caaagcttc ttaagtttg ctcaaaacg 
      ccaaagctg cgccgaaaa accctaata ttaattttc 
      aaatcgatg gtaatgacg ttgtgctac gacgcaatc 
      tctcggaac attgataac gtgggctac tccccattt 
      ctcgttttt acaataacc tcagcaact atactccct 
      gcctgtctt tgcattctg gcggttcaa gaccgaata 
      ggtcgtaaa gctctggca agcggctcg cttcttatg 
      agtggcatt ttcactgca tgctctgga attgttcaa 
      gtgtttatc gaaacccct catccaaca cttatcgta 
      gttcttgcg ataattgca agactcgct gtaacagta 
      gcttataat tccggggct cagtatgcc gttgagtta 
      attccgacc gttgtgaga gcacaggga gtttcaata 
      attcacgta acaggttac gctgcgact tttttcagt 
      ccgcaaatt ttgtatttg gcccgcttt tggaagccc 
      tctcctgaa gttattttg ggggttttg ctagtactt 
      ggcgctggg gcttgttta tttctaccg gaaacgctc 
      aatcggact ttgccggtt acgctgcaa gacggagaa 
      gagttcgga gaggacgaa aatgttttt gattttgct 
      tgctgtaga gcgcccact gagagcacc acacaactg 
      acaagagac cagattcac acgaattat atgtga
    mRNA sequence
    • >TC010761 RA
      tatggatga cgattcaaa aatcactat ctgcacgga 
      gattcaagc gtttgtcta attctcgtt ttacacgaa 
      acattttta tatttaatt taaaactgt tatcaattg 
      tattgtatg aaacgtgat aatgtcgta aatactgtc 
      agttgtaca tataattgt tttgagcgt tttaaaaat 
      ccagtgcgt atgcgagta cgagccagc gtgttttat 
      tttgaataa ttaaaccta ttttcggtc gcgtaagta 
      cgtagacga ccccatcca caaccatta gttaatgac 
      tttagatat aaataaatt tacgagatg actctattt 
      aatctgctt gagttccaa gttattctg ataataaaa 
      tcgaagcat taacctcat ccatttatt taaataaat 
      aaaaacgct gagcagtgc ttcgatttt gtacctaaa 
      gtacagtcc cgagtgttg catgctcgg ccaagtcgt 
      gcgtaaatt tttcaaatg ctttattac ttatacact 
      atttatttc aattgatta cttcctcaa acaacatgt 
      attaatcaa tgttaggaa agcgctgtg ttggagttt 
      ttctgcagc gtttgtgag cgacgagtg gttgagaag 
      tgcgaaaat ggctcaaga tgacttaat taatattga 
      cagcatgct ctcgcatgc cggggattt cggacgaca 
      ccagtgcat cctcatgtt tttgttcag tgtaataaa 
      tgtgctgtc tgcctttca ttacttcgg ccagatttt 
      tatcagtat tgtccccga acacaagtg cagaatgtc 
      cacaaagga agatccgcg gtttattgc cgcggaaca 
      atgttccta cacgatttt tcacgaaga catttacca 
      aaaaatacc ctgctctga agactggga ctacaacaa 
      cagttacgg ctacgtcag tattgttca agagctcga 
      ttgggtgtg tgacgagag ctggaggcc cgctcttgg 
      acaatcagt gttttttat cggatcggt catcgggag 
      cctatttct cggaatcct ggccgatag tatcggcag 
      actacacgt cttggtggc gtcaaattt acttgcctt 
      tttcggaaa catcacgac acttatcac gacaaacac 
      tacgatttt tgcaatgtg tcgcttctt ggccggatg 
      tgcaaccga cgctaattt cgtcatgat gtacattat 
      agttatgga atacattcg gcccagtat gcgcacttt 
      tggcctaaa cctttgcat tggagtttt ctactgttt 
      cgcttgcat ggtaatacc atgggtggc cgtttggtt 
      gcaaaattg ggaatccct tcttctggc gattgcaat 
      tccgcactt atcagttct gagcttcta cttcatagt 
      accagaaag tgcccagtg gttgataag caaaggacg 
      caccgaaga agctattga ttgtttcaa gcgcattgc 
      tcgagttaa tgggaaaaa attaagtga taaacattt 
      cgaaggcct gactcgtta ctgctgcca acacataaa 
      tgcgagaaa aaagcacca aagcttctt aagtttgct 
      caaaacgcc aaagctgcg ccgaaaaac cctaatatt 
      aattttcaa atcgatggt aatgacgtt gtgctacga 
      cgcaatctc tcggaacat tgataacgt gggctactc 
      cccatttct cgtttttac aataacctc agcaactat 
      actccctgc ctgtctttg cattctggc ggttcaaga 
      ccgaatagg tcgtaaagc tctggcaag cggctcgct 
      tcttatgag tggcatttt cactgcatg ctctggaat 
      tgttcaagt gtttatcga aacccctca tccaacact 
      tatcgtagt tcttgcgat aattgcaag actcgctgt 
      aacagtagc ttataattc cggggctca gtatgccgt 
      tgagttaat tccgaccgt tgtgagagc acagggagt 
      ttcaataat tcacgtaac aggttacgc tgcgacttt 
      tttcagtcc gcaaatttt gtatttggc ccgcttttg 
      gaagccctc tcctgaagt tattttggg ggttttgct 
      agtacttgg cgctggggc ttgtttatt tctaccgga 
      aacgctcaa tcggacttt gccggttac gctgcaaga 
      cggagaaga gttcggaga ggacgaaaa tgtttttga 
      ttttgcttg ctgtagagc gcccactga gagcaccac 
      acaactgac aagagacca gattcacac gaattatat 
      gtgagctgt gattaaacg tacttaatt ttgtaaata 
      cattggaaa taaaatttt tttaaatta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY