Status
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Gene information
General information
TC010759 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4803477..4808235
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010759
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01726

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larval musculature partially shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larval musculature partially orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature partiallyrounded - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head appendages shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    gnathal appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    Pupal morphology
    hindwing blistered - Number of animals showing the phenotype: 7

    wings orientation irregular - Number of animals showing the phenotype: 7

    wings blistered - Number of animals showing the phenotype: 1

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010759 RA
      MDPNGNGKR GLPRLREII RRQRQDSDG GGTDSPDLD 
      FIYDDTDTH PNEIAELYS YTEQPELAL NVKAFEDQM 
      EQYNLPPSW QRLSLEQRK SVVMKLLDQ LEVSSKPLR 
      MRAARCILY IAQGCWAEM QSDAEQQQW ARVNVMLLY 
      QLGVFSAFT DLLNIEIEN STAAHVAMR KIAVSLADS 
      QDLRVILSV LYTITEVIR NEKYSGSTE YQSDVESFC 
      SDLANSYDD ELLIVKLLG MVTRFCSGA APHFPMKKV 
      LLLLWKLIL VTLGGMTVL KDLKDEKRE KAGLPRLDE 
      DTMEIAKTM RASSPPASA SDLLEAQNQ KRNTRPFRR 
      NLMKQSSLD DHESLGMEL DATLVGGDN GDELGELSE 
      YDDRRPPEN SENNQMYSN YQNRPTSPP LQSSLVVPR 
      GLPWKPKVR QKDIDLFLD TARLKFVGY SLQGDRDSL 
      AGLPLPIHE GVKTLKEHV YTSLAELQI QKEEEIARN 
      PLSTSEGEI EMTPTEILY QAMLPNLPQ YMIALLKIL 
      LAAAPTSKA KTDSINIMA DVLPEEMPM TVLQSMKLG 
      IDVNRHKEI IVKAVSAIL LLLLKHFKL NHIYQFEFM 
      SQHLVFANC IPLVLKFFN QNIMAYVGA KNVIPILDF 
      PSCVIGDQP ELTAESLEI GDSAPFSWR NMFSCINLL 
      RILNKLTKW KHSRIMMLV VFKSAPILK RTLKVRHAM 
      TQLYVLKLL KMQTKYLGR QWRKSNMKT ISAIYQKVR 
      HRLNDDWAY GNDLDARPW DFQAEECAL RASVDRFNN 
      RRYTQANQD SEFAPVDNC LNSVLGTTY DLSESFKQH 
      YQLWLEQEV YGNTIEWEE LLLNQTI
    CDS sequence
    • >TC010759 RA
      atggatcct aacggaaat ggaaagcgt ggtttacct 
      aggttaaga gagattatt agaagacaa cgccaagat 
      tcggatggt ggtgggact gattccccg gaccttgac 
      tttatttac gatgacacc gacacccat ccgaatgaa 
      attgccgag ctgtacagt tacactgag cagccggag 
      ctcgccctc aatgttaaa gcctttgaa gaccaaatg 
      gagcagtat aacctgcca ccaagttgg caaaggtta 
      tcccttgaa caaagaaaa tcggtagta atgaaactg 
      ctcgatcaa ctggaagtc agcagcaag cctctcagg 
      atgagagct gccagatgt attttgtat attgctcag 
      ggatgttgg gctgaaatg cagtcagac gctgagcaa 
      cagcagtgg gctcgagtc aatgtcatg ttgctttac 
      cagcttgga gtttttagc gcatttact gatttgctt 
      aatatagaa attgagaat agcactgct gcccatgtc 
      gctatgaga aaaatcgcc gtttcgtta gcagattcg 
      caagatctg cgcgttata ttgtctgtg ttatacaca 
      ataactgaa gtaattagg aacgagaaa tattcaggt 
      tcaactgaa taccaaagc gatgttgaa tcgttttgt 
      tccgacctt gctaattcc tacgatgat gagttgctt 
      attgtcaaa ttgttggga atggtgaca cgcttttgt 
      agcggtgca gctcctcat ttcccaatg aaaaaagtt 
      ctgttactg ctttggaaa ttaattctt gttactttg 
      ggcggtatg accgtcttg aaagacttg aaagatgag 
      aagcgcgaa aaagctggt cttccacgt ttggacgag 
      gatactatg gaaatagcc aagacaatg agggcctcc 
      tcgccacca gcgagcgct tctgatctt ctggaggcg 
      caaaatcag aaaagaaac acgagaccg ttcaggagg 
      aacttaatg aaacaaagt agtttggac gaccacgag 
      tcgttgggg atggaatta gacgccacc ctcgttggc 
      ggggacaac ggcgacgag ttgggtgaa ctctccgaa 
      tacgacgat cgtcgtcct cccgaaaac tccgaaaat 
      aatcaaatg tacagcaat tatcagaat aggcctaca 
      tcacctcct ttgcagagt tctttagtt gttcctcgc 
      ggcttacct tggaaaccg aaagtgcgg cagaaagat 
      atcgacttg tttttggac actgctaga ttaaaattc 
      gtcgggtat agtctacaa ggcgatcgc gatagttta 
      gctggacta cctttaccc atccatgaa ggagttaaa 
      actttgaaa gaacatgtt tatacttct ttagctgag 
      ttgcaaatt caaaaagag gaagagatt gctcgtaat 
      cctctctcg acgtccgag ggcgaaata gaaatgact 
      ccaactgaa attttgtat caggcaatg ttgcctaat 
      ctaccacaa tacatgata gctttactt aaaatatta 
      cttgctgct gctcctacc tcaaaagct aaaactgat 
      tctattaat attatggct gatgttttg ccagaagag 
      atgccaatg actgttctc cagtcaatg aaattggga 
      attgacgta aataggcac aaggaaatt atagttaag 
      gcagtttct gcaatttta ttgttactt ctgaaacat 
      ttcaaattg aatcatatt tatcagttt gaattcatg 
      tctcagcat ctagttttt gcaaactgt atacctcta 
      gtattgaaa ttcttcaat cagaacatc atggcttac 
      gtgggcgcg aagaatgtg attcctata ttagatttt 
      ccgtcttgt gtgatcggt gatcaaccg gaactaaca 
      gctgaaagt ctggagatc ggagactca gcaccattt 
      tcctggagg aatatgttc tcatgtatt aatttgtta 
      aggatactc aataagtta acaaaatgg aaacattcc 
      aggattatg atgttggtg gtttttaaa tccgctcca 
      attttaaaa cgcacactc aaagtcagg catgcaatg 
      acccaactg tacgtttta aaattatta aaaatgcaa 
      accaaatac ttgggtcgc caatggcgc aaatctaat 
      atgaaaact attagtgcc atctaccag aaagtgagg 
      catcggttg aacgatgac tgggcatac ggaaatgat 
      ttggacgca agaccctgg gatttccaa gctgaggaa 
      tgtgctttg agagcttca gttgacagg tttaataac 
      cgtagatat acgcaagca aatcaagac agcgaattt 
      gcacctgtg gataactgt ctaaacagc gtgcttggg 
      accacctac gatctctcc gagagtttt aaacaacac 
      taccaactt tggctagaa caagaagtt tatgggaat 
      actatcgaa tgggaggaa cttctctta aaccaaaca 
      atatag
    mRNA sequence
    • >TC010759 RA
      ggtctcccg ttctgcgct gcgccctct ggcgagtat 
      ttttttcgt gaacatacg ttgcaaaaa attaactgc 
      tgcttcgta agtgttttt cttagataa cacctccca 
      gagcaagaa gttgcgata aaatctttg ggttttcgg 
      ggttttccc gattttggc gagaaagga ggcgctcca 
      ggcgtgggt caatatacg tcagtcaag atggcggaa 
      tttgtttaa ctgtcataa aattgttgg tttgcaaat 
      aaatggatc ctaacggaa atggaaagc gtggtttac 
      ctaggttaa gagagatta ttagaagac aacgccaag 
      attcggatg gtggtggga ctgattccc cggaccttg 
      actttattt acgatgaca ccgacaccc atccgaatg 
      aaattgccg agctgtaca gttacactg agcagccgg 
      agctcgccc tcaatgtta aagcctttg aagaccaaa 
      tggagcagt ataacctgc caccaagtt ggcaaaggt 
      tatcccttg aacaaagaa aatcggtag taatgaaac 
      tgctcgatc aactggaag tcagcagca agcctctca 
      ggatgagag ctgccagat gtattttgt atattgctc 
      agggatgtt gggctgaaa tgcagtcag acgctgagc 
      aacagcagt gggctcgag tcaatgtca tgttgcttt 
      accagcttg gagttttta gcgcattta ctgatttgc 
      ttaatatag aaattgaga atagcactg ctgcccatg 
      tcgctatga gaaaaatcg ccgtttcgt tagcagatt 
      cgcaagatc tgcgcgtta tattgtctg tgttataca 
      caataactg aagtaatta ggaacgaga aatattcag 
      gttcaactg aataccaaa gcgatgttg aatcgtttt 
      gttccgacc ttgctaatt cctacgatg atgagttgc 
      ttattgtca aattgttgg gaatggtga cacgctttt 
      gtagcggtg cagctcctc atttcccaa tgaaaaaag 
      ttctgttac tgctttgga aattaattc ttgttactt 
      tgggcggta tgaccgtct tgaaagact tgaaagatg 
      agaagcgcg aaaaagctg gtcttccac gtttggacg 
      aggatacta tggaaatag ccaagacaa tgagggcct 
      cctcgccac cagcgagcg cttctgatc ttctggagg 
      cgcaaaatc agaaaagaa acacgagac cgttcagga 
      ggaacttaa tgaaacaaa gtagtttgg acgaccacg 
      agtcgttgg ggatggaat tagacgcca ccctcgttg 
      gcggggaca acggcgacg agttgggtg aactctccg 
      aatacgacg atcgtcgtc ctcccgaaa actccgaaa 
      ataatcaaa tgtacagca attatcaga ataggccta 
      catcacctc ctttgcaga gttctttag ttgttcctc 
      gcggcttac cttggaaac cgaaagtgc ggcagaaag 
      atatcgact tgtttttgg acactgcta gattaaaat 
      tcgtcgggt atagtctac aaggcgatc gcgatagtt 
      tagctggac tacctttac ccatccatg aaggagtta 
      aaactttga aagaacatg tttatactt ctttagctg 
      agttgcaaa ttcaaaaag aggaagaga ttgctcgta 
      atcctctct cgacgtccg agggcgaaa tagaaatga 
      ctccaactg aaattttgt atcaggcaa tgttgccta 
      atctaccac aatacatga tagctttac ttaaaatat 
      tacttgctg ctgctccta cctcaaaag ctaaaactg 
      attctatta atattatgg ctgatgttt tgccagaag 
      agatgccaa tgactgttc tccagtcaa tgaaattgg 
      gaattgacg taaataggc acaaggaaa ttatagtta 
      aggcagttt ctgcaattt tattgttac ttctgaaac 
      atttcaaat tgaatcata tttatcagt ttgaattca 
      tgtctcagc atctagttt ttgcaaact gtatacctc 
      tagtattga aattcttca atcagaaca tcatggctt 
      acgtgggcg cgaagaatg tgattccta tattagatt 
      ttccgtctt gtgtgatcg gtgatcaac cggaactaa 
      cagctgaaa gtctggaga tcggagact cagcaccat 
      tttcctgga ggaatatgt tctcatgta ttaatttgt 
      taaggatac tcaataagt taacaaaat ggaaacatt 
      ccaggatta tgatgttgg tggttttta aatccgctc 
      caattttaa aacgcacac tcaaagtca ggcatgcaa 
      tgacccaac tgtacgttt taaaattat taaaaatgc 
      aaaccaaat acttgggtc gccaatggc gcaaatcta 
      atatgaaaa ctattagtg ccatctacc agaaagtga 
      ggcatcggt tgaacgatg actgggcat acggaaatg 
      atttggacg caagaccct gggatttcc aagctgagg 
      aatgtgctt tgagagctt cagttgaca ggtttaata 
      accgtagat atacgcaag caaatcaag acagcgaat 
      ttgcacctg tggataact gtctaaaca gcgtgcttg 
      ggaccacct acgatctct ccgagagtt ttaaacaac 
      actaccaac tttggctag aacaagaag tttatggga 
      atactatcg aatgggagg aacttctct taaaccaaa 
      caatatagt gactttgat attatttat tgaactatt 
      gcagtaaat ataacacca tcacaattt tcatcactg 
      caataaata tactcagtt tcaattaag taagtggtg 
      tgttacatt tacggcatt tttgtattt ttttgttcg 
      ttttagttc agtttttgt gttctagtt agtattcaa 
      tgtttcgtt ttgtttttg acaattcct attgaatta 
      actttgtta tccgcacca gttcttatt tgttggaaa 
      ataattccc cagttaaaa aaattttgg ttacttcag 
      tatttaaat atatttgct atataatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY