Status
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Gene information
General information
TC010759 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4803477..4808235
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010759
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01726

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larval musculature partially shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larval musculature partially orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature partiallyrounded - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     head appendages shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    gnathal appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection

    Pupal morphology
    hindwing blistered - Number of animals showing the phenotype: 7

    wings orientation irregular - Number of animals showing the phenotype: 7

    wings blistered - Number of animals showing the phenotype: 1

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010759 RA
      MDPNGNGKR GLPRLREII RRQRQDSDG GGTDSPDLD 
FIYDDTDTH PNEIAELYS YTEQPELAL NVKAFEDQM 
EQYNLPPSW QRLSLEQRK SVVMKLLDQ LEVSSKPLR 
MRAARCILY IAQGCWAEM QSDAEQQQW ARVNVMLLY 
QLGVFSAFT DLLNIEIEN STAAHVAMR KIAVSLADS 
QDLRVILSV LYTITEVIR NEKYSGSTE YQSDVESFC 
SDLANSYDD ELLIVKLLG MVTRFCSGA APHFPMKKV 
LLLLWKLIL VTLGGMTVL KDLKDEKRE KAGLPRLDE 
DTMEIAKTM RASSPPASA SDLLEAQNQ KRNTRPFRR 
NLMKQSSLD DHESLGMEL DATLVGGDN GDELGELSE 
YDDRRPPEN SENNQMYSN YQNRPTSPP LQSSLVVPR 
GLPWKPKVR QKDIDLFLD TARLKFVGY SLQGDRDSL 
AGLPLPIHE GVKTLKEHV YTSLAELQI QKEEEIARN 
PLSTSEGEI EMTPTEILY QAMLPNLPQ YMIALLKIL 
LAAAPTSKA KTDSINIMA DVLPEEMPM TVLQSMKLG 
IDVNRHKEI IVKAVSAIL LLLLKHFKL NHIYQFEFM 
SQHLVFANC IPLVLKFFN QNIMAYVGA KNVIPILDF 
PSCVIGDQP ELTAESLEI GDSAPFSWR NMFSCINLL 
RILNKLTKW KHSRIMMLV VFKSAPILK RTLKVRHAM 
TQLYVLKLL KMQTKYLGR QWRKSNMKT ISAIYQKVR 
HRLNDDWAY GNDLDARPW DFQAEECAL RASVDRFNN 
RRYTQANQD SEFAPVDNC LNSVLGTTY DLSESFKQH 
YQLWLEQEV YGNTIEWEE LLLNQTI
    CDS sequence
    • >TC010759 RA
      atggatcct aacggaaat ggaaagcgt ggtttacct 
aggttaaga gagattatt agaagacaa cgccaagat 
tcggatggt ggtgggact gattccccg gaccttgac 
tttatttac gatgacacc gacacccat ccgaatgaa 
attgccgag ctgtacagt tacactgag cagccggag 
ctcgccctc aatgttaaa gcctttgaa gaccaaatg 
gagcagtat aacctgcca ccaagttgg caaaggtta 
tcccttgaa caaagaaaa tcggtagta atgaaactg 
ctcgatcaa ctggaagtc agcagcaag cctctcagg 
atgagagct gccagatgt attttgtat attgctcag 
ggatgttgg gctgaaatg cagtcagac gctgagcaa 
cagcagtgg gctcgagtc aatgtcatg ttgctttac 
cagcttgga gtttttagc gcatttact gatttgctt 
aatatagaa attgagaat agcactgct gcccatgtc 
gctatgaga aaaatcgcc gtttcgtta gcagattcg 
caagatctg cgcgttata ttgtctgtg ttatacaca 
ataactgaa gtaattagg aacgagaaa tattcaggt 
tcaactgaa taccaaagc gatgttgaa tcgttttgt 
tccgacctt gctaattcc tacgatgat gagttgctt 
attgtcaaa ttgttggga atggtgaca cgcttttgt 
agcggtgca gctcctcat ttcccaatg aaaaaagtt 
ctgttactg ctttggaaa ttaattctt gttactttg 
ggcggtatg accgtcttg aaagacttg aaagatgag 
aagcgcgaa aaagctggt cttccacgt ttggacgag 
gatactatg gaaatagcc aagacaatg agggcctcc 
tcgccacca gcgagcgct tctgatctt ctggaggcg 
caaaatcag aaaagaaac acgagaccg ttcaggagg 
aacttaatg aaacaaagt agtttggac gaccacgag 
tcgttgggg atggaatta gacgccacc ctcgttggc 
ggggacaac ggcgacgag ttgggtgaa ctctccgaa 
tacgacgat cgtcgtcct cccgaaaac tccgaaaat 
aatcaaatg tacagcaat tatcagaat aggcctaca 
tcacctcct ttgcagagt tctttagtt gttcctcgc 
ggcttacct tggaaaccg aaagtgcgg cagaaagat 
atcgacttg tttttggac actgctaga ttaaaattc 
gtcgggtat agtctacaa ggcgatcgc gatagttta 
gctggacta cctttaccc atccatgaa ggagttaaa 
actttgaaa gaacatgtt tatacttct ttagctgag 
ttgcaaatt caaaaagag gaagagatt gctcgtaat 
cctctctcg acgtccgag ggcgaaata gaaatgact 
ccaactgaa attttgtat caggcaatg ttgcctaat 
ctaccacaa tacatgata gctttactt aaaatatta 
cttgctgct gctcctacc tcaaaagct aaaactgat 
tctattaat attatggct gatgttttg ccagaagag 
atgccaatg actgttctc cagtcaatg aaattggga 
attgacgta aataggcac aaggaaatt atagttaag 
gcagtttct gcaatttta ttgttactt ctgaaacat 
ttcaaattg aatcatatt tatcagttt gaattcatg 
tctcagcat ctagttttt gcaaactgt atacctcta 
gtattgaaa ttcttcaat cagaacatc atggcttac 
gtgggcgcg aagaatgtg attcctata ttagatttt 
ccgtcttgt gtgatcggt gatcaaccg gaactaaca 
gctgaaagt ctggagatc ggagactca gcaccattt 
tcctggagg aatatgttc tcatgtatt aatttgtta 
aggatactc aataagtta acaaaatgg aaacattcc 
aggattatg atgttggtg gtttttaaa tccgctcca 
attttaaaa cgcacactc aaagtcagg catgcaatg 
acccaactg tacgtttta aaattatta aaaatgcaa 
accaaatac ttgggtcgc caatggcgc aaatctaat 
atgaaaact attagtgcc atctaccag aaagtgagg 
catcggttg aacgatgac tgggcatac ggaaatgat 
ttggacgca agaccctgg gatttccaa gctgaggaa 
tgtgctttg agagcttca gttgacagg tttaataac 
cgtagatat acgcaagca aatcaagac agcgaattt 
gcacctgtg gataactgt ctaaacagc gtgcttggg 
accacctac gatctctcc gagagtttt aaacaacac 
taccaactt tggctagaa caagaagtt tatgggaat 
actatcgaa tgggaggaa cttctctta aaccaaaca 
atatag
    mRNA sequence
    • >TC010759 RA
      ggtctcccg ttctgcgct gcgccctct ggcgagtat 
ttttttcgt gaacatacg ttgcaaaaa attaactgc 
tgcttcgta agtgttttt cttagataa cacctccca 
gagcaagaa gttgcgata aaatctttg ggttttcgg 
ggttttccc gattttggc gagaaagga ggcgctcca 
ggcgtgggt caatatacg tcagtcaag atggcggaa 
tttgtttaa ctgtcataa aattgttgg tttgcaaat 
aaatggatc ctaacggaa atggaaagc gtggtttac 
ctaggttaa gagagatta ttagaagac aacgccaag 
attcggatg gtggtggga ctgattccc cggaccttg 
actttattt acgatgaca ccgacaccc atccgaatg 
aaattgccg agctgtaca gttacactg agcagccgg 
agctcgccc tcaatgtta aagcctttg aagaccaaa 
tggagcagt ataacctgc caccaagtt ggcaaaggt 
tatcccttg aacaaagaa aatcggtag taatgaaac 
tgctcgatc aactggaag tcagcagca agcctctca 
ggatgagag ctgccagat gtattttgt atattgctc 
agggatgtt gggctgaaa tgcagtcag acgctgagc 
aacagcagt gggctcgag tcaatgtca tgttgcttt 
accagcttg gagttttta gcgcattta ctgatttgc 
ttaatatag aaattgaga atagcactg ctgcccatg 
tcgctatga gaaaaatcg ccgtttcgt tagcagatt 
cgcaagatc tgcgcgtta tattgtctg tgttataca 
caataactg aagtaatta ggaacgaga aatattcag 
gttcaactg aataccaaa gcgatgttg aatcgtttt 
gttccgacc ttgctaatt cctacgatg atgagttgc 
ttattgtca aattgttgg gaatggtga cacgctttt 
gtagcggtg cagctcctc atttcccaa tgaaaaaag 
ttctgttac tgctttgga aattaattc ttgttactt 
tgggcggta tgaccgtct tgaaagact tgaaagatg 
agaagcgcg aaaaagctg gtcttccac gtttggacg 
aggatacta tggaaatag ccaagacaa tgagggcct 
cctcgccac cagcgagcg cttctgatc ttctggagg 
cgcaaaatc agaaaagaa acacgagac cgttcagga 
ggaacttaa tgaaacaaa gtagtttgg acgaccacg 
agtcgttgg ggatggaat tagacgcca ccctcgttg 
gcggggaca acggcgacg agttgggtg aactctccg 
aatacgacg atcgtcgtc ctcccgaaa actccgaaa 
ataatcaaa tgtacagca attatcaga ataggccta 
catcacctc ctttgcaga gttctttag ttgttcctc 
gcggcttac cttggaaac cgaaagtgc ggcagaaag 
atatcgact tgtttttgg acactgcta gattaaaat 
tcgtcgggt atagtctac aaggcgatc gcgatagtt 
tagctggac tacctttac ccatccatg aaggagtta 
aaactttga aagaacatg tttatactt ctttagctg 
agttgcaaa ttcaaaaag aggaagaga ttgctcgta 
atcctctct cgacgtccg agggcgaaa tagaaatga 
ctccaactg aaattttgt atcaggcaa tgttgccta 
atctaccac aatacatga tagctttac ttaaaatat 
tacttgctg ctgctccta cctcaaaag ctaaaactg 
attctatta atattatgg ctgatgttt tgccagaag 
agatgccaa tgactgttc tccagtcaa tgaaattgg 
gaattgacg taaataggc acaaggaaa ttatagtta 
aggcagttt ctgcaattt tattgttac ttctgaaac 
atttcaaat tgaatcata tttatcagt ttgaattca 
tgtctcagc atctagttt ttgcaaact gtatacctc 
tagtattga aattcttca atcagaaca tcatggctt 
acgtgggcg cgaagaatg tgattccta tattagatt 
ttccgtctt gtgtgatcg gtgatcaac cggaactaa 
cagctgaaa gtctggaga tcggagact cagcaccat 
tttcctgga ggaatatgt tctcatgta ttaatttgt 
taaggatac tcaataagt taacaaaat ggaaacatt 
ccaggatta tgatgttgg tggttttta aatccgctc 
caattttaa aacgcacac tcaaagtca ggcatgcaa 
tgacccaac tgtacgttt taaaattat taaaaatgc 
aaaccaaat acttgggtc gccaatggc gcaaatcta 
atatgaaaa ctattagtg ccatctacc agaaagtga 
ggcatcggt tgaacgatg actgggcat acggaaatg 
atttggacg caagaccct gggatttcc aagctgagg 
aatgtgctt tgagagctt cagttgaca ggtttaata 
accgtagat atacgcaag caaatcaag acagcgaat 
ttgcacctg tggataact gtctaaaca gcgtgcttg 
ggaccacct acgatctct ccgagagtt ttaaacaac 
actaccaac tttggctag aacaagaag tttatggga 
atactatcg aatgggagg aacttctct taaaccaaa 
caatatagt gactttgat attatttat tgaactatt 
gcagtaaat ataacacca tcacaattt tcatcactg 
caataaata tactcagtt tcaattaag taagtggtg 
tgttacatt tacggcatt tttgtattt ttttgttcg 
ttttagttc agtttttgt gttctagtt agtattcaa 
tgtttcgtt ttgtttttg acaattcct attgaatta 
actttgtta tccgcacca gttcttatt tgttggaaa 
ataattccc cagttaaaa aaattttgg ttacttcag 
tatttaaat atatttgct atataatg

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY