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Gene information
General information
TC010758 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4800857..4803388
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010758
    iBeetle screen iB_01725

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head capsule slightly size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: Bristle pattern possible impaired
    antenna elongated - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    maxilla size increased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    labrum elongated - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010758 RA
      MWGTTKLSE RVPALHKIS QDSFKDTLL EYLKEDPYI 
      IVFVEPTLS PEDFAQHDQ NGDIAFPNL HGLKKLKNH 
      VAYKPYVQN PVRAVKQLN KEVTELSIA SLLSSSNVP 
      KDNILIIDL NDAKDDEPR FHMLKRHDS DIVSIYKDI 
      LEQRNNVLA IYTANHTSW IAPEDITHS RSRSLLQSE 
      DTEEKDTGH LYTSEHVLL YLSKDGMYQ VDKESSGIL 
      IDDSFTLHE ETTSKDNKE NITAILSSG KSDLSVNFD 
      IKFNNVSGY WYLTSMSVK KGGKEDPIV GISDYFAPR 
      GFSYHCSTF NLSTTDKNA TLSLPGFQI QPFPKNNTF 
      GDAYDCVGF TSVPIWSGL FITIILLLI VTFGLTMMM 
      DIKTMDRFD DPKGKTITI TASE
    CDS sequence
    • >TC010758 RA
      atgtggggg actacgaag ttgtcagaa cgagtacct 
      gctttgcac aaaatcagt caagattcg tttaaagac 
      accctttta gagtatctg aaggaggac ccttacata 
      attgtgttt gtagaaccc actttgagt cctgaagat 
      tttgctcaa catgaccaa aacggggat atcgcgttc 
      ccgaatctc cacggtttg aagaaactt aaaaatcac 
      gttgcttac aaaccctat gtacaaaat ccagttaga 
      gccgtcaaa cagctcaat aaggaagtc acagagttg 
      tccattgca tctctgttg agtagctct aatgtgcct 
      aaagataat attttgatt attgatttg aacgatgcc 
      aaagatgat gaaccccgg tttcatatg ctgaaacgt 
      cacgattct gacattgtg tcaatttat aaggatatc 
      ctagaacaa cgcaataat gtcttggct atatatacg 
      gcgaatcac acgtcttgg atagcgccc gaggatatt 
      acccactcc agatccaga agcttgctc cagtcagaa 
      gatacagaa gagaaagac actggacac ttatatacc 
      tccgaacac gttttgtta tatttgagc aaagatgga 
      atgtatcag gtggataag gagtcgtct ggaatccta 
      attgatgac tcttttaca ctacatgaa gaaaccacc 
      agcaaagat aacaaagag aatataaca gcgatttta 
      agcagcggc aagagtgac ttgtctgtt aattttgat 
      atcaaattt aataacgtg tctggatac tggtattta 
      acttcaatg agcgtaaag aaaggtggt aaagaggac 
      ccaatagtt ggtattagt gattacttc gcgcctcgc 
      ggtttttcc taccactgt tctactttt aatttatca 
      acgacggac aaaaacgca acgttgtct ctgcccgga 
      tttcagatc cagccgttc cccaaaaac aacacattt 
      ggggacgcc tatgactgc gttggattc acatcagtt 
      cctatctgg tcgggtttg tttataaca ataatcctg 
      ttgttgatc gtcaccttt ggtcttacc atgatgatg 
      gatattaag actatggac cgattcgat gaccccaaa 
      ggcaaaacg atcactatc actgcttct gaataa
    mRNA sequence
    • >TC010758 RA
      taaggtcgc ttgacgtcg ctcgcctcg ctagtcatt 
      cgttacacc tgattgtca aatgtttgc tactcccaa 
      aattcacgt gatcttgta aaagtgaag tttaatttt 
      attttttag cagaatggc tctttattg taacatttt 
      aatatttgt aataacagt attaggcat aaatgttgt 
      cagaagtgc agaatttgt acctgttta catgtgggg 
      gactacgaa gttgtcaga acgagtacc tgctttgca 
      caaaatcag tcaagattc gtttaaaga caccctttt 
      agagtatct gaaggagga cccttacat aattgtgtt 
      tgtagaacc cactttgag tcctgaaga ttttgctca 
      acatgacca aaacgggga tatcgcgtt cccgaatct 
      ccacggttt gaagaaact taaaaatca cgttgctta 
      caaacccta tgtacaaaa tccagttag agccgtcaa 
      acagctcaa taaggaagt cacagagtt gtccattgc 
      atctctgtt gagtagctc taatgtgcc taaagataa 
      tattttgat tattgattt gaacgatgc caaagatga 
      tgaaccccg gtttcatat gctgaaacg tcacgattc 
      tgacattgt gtcaattta taaggatat cctagaaca 
      acgcaataa tgtcttggc tatatatac ggcgaatca 
      cacgtcttg gatagcgcc cgaggatat tacccactc 
      cagatccag aagcttgct ccagtcaga agatacaga 
      agagaaaga cactggaca cttatatac ctccgaaca 
      cgttttgtt atatttgag caaagatgg aatgtatca 
      ggtggataa ggagtcgtc tggaatcct aattgatga 
      ctcttttac actacatga agaaaccac cagcaaaga 
      taacaaaga gaatataac agcgatttt aagcagcgg 
      caagagtga cttgtctgt taattttga tatcaaatt 
      taataacgt gtctggata ctggtattt aacttcaat 
      gagcgtaaa gaaaggtgg taaagagga cccaatagt 
      tggtattag tgattactt cgcgcctcg cggtttttc 
      ctaccactg ttctacttt taatttatc aacgacgga 
      caaaaacgc aacgttgtc tctgcccgg atttcagat 
      ccagccgtt ccccaaaaa caacacatt tggggacgc 
      ctatgactg cgttggatt cacatcagt tcctatctg 
      gtcgggttt gtttataac aataatcct gttgttgat 
      cgtcacctt tggtcttac catgatgat ggatattaa 
      gactatgga ccgattcga tgaccccaa aggcaaaac 
      gatcactat cactgcttc tgaataatg ttggtgata 
      ttgcgtgtt caacattca atattaccg tttagttac 
      gtcttccac actattgca cctgaacag aattaactt 
      ctggctggt tcttgtgat aaataaaaa attcggttt 
      tgttttacc atccatttc tgtcaggtc gcaatactc 
      ggcttaact gccatgtgg tttgtcttc aatttttag 
      gacctaaag cttgttata caatgaaat actccatca 
      acgcgtaac taacgaact agtgtcctg cagagggaa 
      gacctaatt tatatacac agtttatat tatatgtat 
      atatgacaa tagtatttt taacatgtt ttaaatttc 
      atatgaatg gttaactgt tgttcaggt agttgtaca 
      ataattgtt aacagaagt agccttgac gttgttaaa 
      aataagctt accttaggt tggcaccac tgtgcacac 
      tatatacac cccaattgt ccttatttg ttgaatatg 
      ctagataca taggatata catattttg tagccctgt 
      tattgtata gtactgtat tatatgtta aaagtgacc 
      ttagttcca tttcattcc agaaatttc atcgtcatt 
      tttagaatg aataagaac gatttattt ttaaacgca 
      actagactt ggtcgttat ttatgttaa gggtgatgg 
      gctgttgca gtttaccat tgtaaatac cttaggtta 
      tttcattgt catagtgat tatcatgta aggccaaaa 
      atgtaggta gaaacgctg ttaataaat tataccaat 
      agtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY