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Gene information
General information
TC010657 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid67: 491723..521776
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010657
    iBeetle screen iB_01722
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    beetle stock: bad (increased lethality),
    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    appendages partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva cuticle dorsal irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs segment shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment potentially size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Pupal morphology
    elytra ventralorientation not closed - Number of animals showing the phenotype: 9

    longitudinal dorsal thoracic muscles number irregular - Number of animals showing the phenotype: 4

    oblique dorsal thoracic muscles partiallyshortened - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010657 RA
      MADLVYYYV CFVLLLLLS LQQDVAGST ERTEQHPAR 
      DFSRHTLVK PRIYHGREK RQISTSKDE NGPYHLDHL 
      TISLPIDGE DFLLDLTLN RQLIPKGFF RKVQQNGTH 
      QVIRPRPEE VDLCHYGGK VRNKPGSWV ALSTCDGLS 
      GVVFDGEEM HYVENKDGD LHYLYKHAD LVEGNKTCG 
      YAGDAVDPQ KSHNHNRIL RYKRAAEQN SAVRGPYNA 
      NKKSKYVEL VLVVDNREY KELGESTSK VEHHCKTIA 
      NIINGLYAP LNIFIALVG VVIWTEHDE ISFSPNGDT 
      TLTSFLHYR RNKLVKEHP NDNAQLLTK YNFDNGVVG 
      KALKGPICT YEYSGGVNT DHSPVVGLV ATTVAHEMG 
      HNFGMEHDT NECTCPDDR CIMAPSSST VAPTHWSSC 
      SLNYLLLAF THGMDYCLK NKPTALFDS PVCGNGFVE 
      PGEQCDCGL PEHCDNTCC NATTCMLHT NASCATGEC 
      CDLTTCKPK SAGTLCRSA DYECDLPEY CTGHSEYCP 
      ADIYKMDAE MCDGGKAFC YHGFCRTRT DQCKLLWGE 
      TGKSSDDQC YKMNIKGNR HGNCGYDKF NKSFFKCND 
      ENVLCGMLH CKHLNERLE FGMESVSIL SHSFLNLKG 
      SIVACRTAI VDLGINQVD PGLAPDGAK CGEGKMCVN 
      QKCMSVASL QLAGKTCPH NCNNNGWCN NLGHCHCKD 
      GYAPPFCED PGPGGSEDS GPASDPDAR KEFVVALFV 
      IFLGIIPGL VLIAFLTYY IKHNVLHIR SKSAAPTTK 
      SPAKTRTAP FGPDSTKRT EDNHSLLQN DPPTSPLQC 
      EFFGYFKGF SITPLKKVE NQPARAAPP PPIIPPSVP 
      PPPKNNFLT RSMSRSHSF KQALAGVSQ NVSAPTLPP 
      PNPGSTAKP IISSPILEN STCTAKELI SPLRNAPKV 
      PVRPAPEAP PTKDGNTLN RITSFLKKT TPAGSTTSS 
      STSTSTLPR KASKIMDKN ALRSIEISN PILQPEGTT 
      VSVGGEGNK AVIMRAQSM RGTSVTPRP NIQTFGSMR 
      QPGGARRPL SIPSGSRPK SPPPPAPPT SDKKLQIPS 
      LPGYQKPTV KTSQNQYDD CLNKTAPLA KLTEELSSD 
      NIYAVIEES PVTPPEDKP KTSSSSNES MGLLGEIVS 
      EIQNRNFDS IYSTSTLAR KKKQEEEAK IKSPESDTY 
      VNTPYKTPE SVYSNMTSS TSSGYILPS AVNPPIKSP 
      EEPKLSTFK AKSRSPTPT KRAPNKTAI NRQTTPPNL 
      RTRKPSPVR PAPGAPKPR PAPNSPDVV TSCNTKPGN 
      KGPDVVNTK KPTIATTKP NVNGLPRGA VKPKVSFNK 
      PGERKVATV SKSQSDAKP VPSGVRNAA RSSSNVASL 
      QQKFETKTL NAKSNKA
    • >TC010657 RB
      MADLVYYYV CFVLLLLLS LQQDVAGST ERTEQHPAR 
      DFSRHTLVK PRIYHGREK RQISTSKDE NGPYHLDHL 
      TISLPIDGE DFLLDLTLN RQLIPKGFF RKVQQNGTH 
      QVIRPRPEE VDLCHYGGK VRNKPGSWV ALSTCDGLS 
      GVVFDGEEM HYVENKDGD LHYLYKHAD LVEGNKTCG 
      YAGDAVDPQ KSHNHNRIL RYKRAAEQN SAVRGPYNA 
      NKKSKYVEL VLVVDNREY KELGESTSK VEHHCKTIA 
      NIINGLYAP LNIFIALVG VVIWTEHDE ISFSPNGDT 
      TLTSFLHYR RNKLVKEHP NDNAQLLTK YNFDNGVVG 
      KALKGPICT YEYSGGVNT DHSPVVGLV ATTVAHEMG 
      HNFGMEHDT NECTCPDDR CIMAPSSST VAPTHWSSC 
      SLNYLLLAF THGMDYCLK NKPTALFDS PVCGNGFVE 
      PGEQCDCGL PEHCDNTCC NATTCMLHT NASCATGEC 
      CDLTTCKPK SAGTLCRSA DYECDLPEY CTGHSEYCP 
      ADIYKMDAE MCDGGKAFC YHGFCRTRT DQCKLLWGE 
      TGKSSDDQC YKMNIKGNR HGNCGYDKF NKSFFKCND 
      ENVLCGMLH CKHLNERLE FGMESVSIL SHSFLNLKG 
      SIVACRTAI VDLGINQVD PGLAPDGAK CGEGKMCVN 
      QKCMSVASL QLAGKTCPH NCNNNGWCN NLGHCHCKD 
      GYAPPFCED PGPGGSEDS GPASDPDAR KEFVVALFV 
      IFLGIIPGL VLIAFLTYY IKHNVLHIR SKSAAPTYV 
      KNCLFNSFQ HVVSACRGG SLFSNSIPT VSERVQNIR 
      NLEIKQTGL ISTTNTDAI IRSNTITNG TNPESGGTR 
      RLSLRNIKG LKLNTNLRR KDRSSQIET PVTTETLCD 
      TPHMVVSVK DLAKKFNDT SPKV
    CDS sequence
    • >TC010657 RA
      atggcggat ttagtgtac tattatgtt tgctttgtg 
      ttgttgttg ttgttatcc ttgcagcag gatgtagct 
      ggatccact gaaagaact gaacaacat cctgccaga 
      gacttcagc cgccatacg ctcgtcaag cctcggatt 
      taccacgga cgggagaaa cgtcagatc tcgacaagc 
      aaagacgag aatggacca tatcacctc gaccacttg 
      accatatca ctgccaatc gacggcgaa gacttcctc 
      ctagacttg accctgaat cgacagctc attccgaaa 
      ggattcttc cgcaaggtt cagcaaaac ggaacgcac 
      caagtcata cgacccaga ccggaggag gtcgacctg 
      tgccactat gggggcaaa gtcaggaac aaaccgggc 
      tcgtgggtc gccctgtcc acctgcgac gggctctcg 
      ggggtggtg tttgacggg gaggaaatg cactacgtt 
      gagaataaa gacggggat ttgcactac ttgtacaag 
      catgcggac ctggtggag gggaacaag acatgtggg 
      tatgcgggg gacgcagtg gacccccag aagagccac 
      aatcacaac aggatactt aggtataag agagccgcg 
      gagcagaac agtgccgtg aggggtccc tacaacgct 
      aacaagaag tcgaaatac gtcgaattg gttctagtt 
      gtggacaac cgcgagtac aaagaactg ggtgagagc 
      acaagtaaa gtggaacac cattgcaaa accattgcc 
      aacataata aacgggctg tacgcccct cttaacata 
      ttcatagct ttagtcggt gtggtcatt tggacggaa 
      cacgacgaa atcagtttt tcccccaac ggcgacacc 
      accctcacc agcttcctc cactaccga cgcaacaaa 
      ctagtcaag gagcacccc aacgacaac gcccaactc 
      ttaacgaaa tacaatttc gacaacggc gtcgtgggg 
      aaggccctc aaaggcccg atttgtact tatgagtac 
      tcggggggc gtcaacacg gaccactcc cccgtcgtg 
      gggctcgtc gccaccaca gtggcccac gaaatgggg 
      cacaatttc ggcatggag cacgacacg aatgagtgc 
      acgtgtccg gacgaccgg tgcattatg gcccccagt 
      agctcgaca gtggcgcca acacattgg tcctcctgc 
      agcctcaac tacctccta cttgcattc acacacggc 
      atggactac tgtctcaag aacaaaccg acggcgctg 
      tttgatagc cccgtctgt ggcaacggt ttcgtcgaa 
      ccgggggaa cagtgcgat tgtgggctt ccggagcac 
      tgtgacaac acttgttgc aatgccacc acgtgtatg 
      ttgcacacg aatgcgagc tgtgcgacg ggagaatgc 
      tgcgattta acaacttgt aaaccgaaa tcggcgggt 
      acgttgtgt cgatcggcc gattacgaa tgcgatttg 
      cccgaatat tgcaccggt catagtgaa tattgcccg 
      gctgatatc tacaaaatg gatgctgaa atgtgtgat 
      ggcgggaag gcgttttgc tatcatggc ttttgcagg 
      actaggact gatcagtgt aaacttttg tggggcgaa 
      acgggcaaa tccagtgac gaccagtgt tacaaaatg 
      aatattaaa ggaaatcga catggaaat tgcggttat 
      gataagttc aacaagagt ttcttcaag tgcaacgat 
      gagaacgtc ttgtgcgga atgcttcat tgcaagcat 
      ttgaacgag cgtttggaa ttcggcatg gaatcggtg 
      tctattttg agccacagt ttcctaaac ttgaaaggt 
      tcaatagtg gcctgtcgg actgccatc gtcgacttg 
      ggcatcaac caagtggat ccaggtctc gcaccagat 
      ggcgccaaa tgtggtgag ggcaagatg tgcgtgaac 
      cagaagtgt atgtccgtg gctagtctc caactagct 
      ggaaaaact tgccctcac aattgcaac aacaacggt 
      tggtgcaac aatttgggc cactgccac tgtaaagat 
      ggctacgct ccgccattt tgcgaggat ccgggacct 
      gggggatcg gaggacagc ggcccagct tcggatcct 
      gacgctcgc aaagagttt gttgtggct ctttttgtg 
      atattcttg gggattatt cccggtttg gtactgatc 
      gcgttcctt acttattat atcaaacac aacgttctg 
      cacattcgt tcgaaatca gcggcccca acgaccaag 
      tcgcctgcg aaaacacgc accgccccg ttcggcccc 
      gattccacc aaacggacc gaagacaac cactccctc 
      ctgcaaaac gacccgccc acttcgccc ctacagtgc 
      gagtttttc ggctatttt aaaggtttc tccataaca 
      ccattaaag aaagtcgaa aatcagcca gcaagggcg 
      gcacccccg cccccgatc ataccccca tcggttcca 
      ccgccacct aaaaacaat tttttgaca cgctccatg 
      tccagatct cactcattt aagcaagct ttggccggc 
      gtctctcag aatgtgagt gcgccgacg ctaccgccc 
      cctaacccc ggcagcacg gctaaacca atcatatcg 
      tcgccgatt ttggagaat tcaacttgc acggccaaa 
      gaattaatc tcgccgttg aggaacgcc cccaaagtg 
      ccggtaagg cccgccccc gaggccccg cccacaaaa 
      gatggcaat acgctaaat agaataact tcgttcttg 
      aagaaaact actcctgct ggcagtact actagtagt 
      agcactagt actagtact ttgcccaga aaggcgagt 
      aaaattatg gataagaac gcactgaga tcgattgaa 
      atttcaaat ccgattcta caacccgaa ggcaccaca 
      gttagtgtt ggaggggag ggtaataag gcggtgata 
      atgagggcg caaagtatg agaggcact agtgtgacc 
      cctagacct aatatacaa actttcggg tcaatgaga 
      caaccagga ggtgccagg cgccccttg agcatacct 
      tcagggagt cgccctaag agccccccg cctccggcc 
      ccgcccact tctgacaaa aaattacaa atacccagc 
      ctgcctgga taccaaaaa ccaacagtc aaaacgagc 
      caaaaccaa tatgacgat tgtttaaac aaaacagca 
      cctttagca aaactaacc gaagaacta agcagtgat 
      aacatttac gcagtgatt gaagaatca ccagtcacg 
      ccccctgaa gacaagccc aagaccagt tcaagttcg 
      aacgaaagc atgggcctc ctcggagaa atagtctca 
      gaaatccaa aatcgcaat tttgactca atttactca 
      acttcgacc ctcgcccgc aagaagaaa caagaagag 
      gaagcaaaa atcaaaagc cccgaatcg gacacgtac 
      gtcaacacc ccgtacaaa accccggaa agcgtctac 
      agcaacatg actagctcc acctccagc ggctacatc 
      ctcccatcg gcggtcaac ccgccaatc aaatcgccc 
      gaggaaccc aaactctcc accttcaaa gcgaagtcg 
      cgcagcccc accccgacc aagcgcgcg cccaataaa 
      accgcgatc aatcgccag accacgccc ccgaatctg 
      cgcacgcgc aagccgtcg ccggttagg cccgccccc 
      ggggccccg aagccacgc ccagcgccc aacagcccg 
      gatgtcgtg acgagttgc aacaccaaa cccgggaat 
      aagggaccg gatgttgtc aatacgaag aaaccgacg 
      attgcgacc accaaaccg aatgttaat ggattgccc 
      agaggggcg gttaaaccg aaagttagt ttcaataaa 
      ccgggggag aggaaggtg gctacggtt agtaagagc 
      caatcggat gcgaagcct gtgccgtca ggggtcagg 
      aacgcggcg aggtcgtcg tcaaatgtg gcgtcgctg 
      cagcagaaa ttcgagact aagacttta aacgcgaaa 
      agtaacaag gcgtga
    • >TC010657 RB
      atggcggat ttagtgtac tattatgtt tgctttgtg 
      ttgttgttg ttgttatcc ttgcagcag gatgtagct 
      ggatccact gaaagaact gaacaacat cctgccaga 
      gacttcagc cgccatacg ctcgtcaag cctcggatt 
      taccacgga cgggagaaa cgtcagatc tcgacaagc 
      aaagacgag aatggacca tatcacctc gaccacttg 
      accatatca ctgccaatc gacggcgaa gacttcctc 
      ctagacttg accctgaat cgacagctc attccgaaa 
      ggattcttc cgcaaggtt cagcaaaac ggaacgcac 
      caagtcata cgacccaga ccggaggag gtcgacctg 
      tgccactat gggggcaaa gtcaggaac aaaccgggc 
      tcgtgggtc gccctgtcc acctgcgac gggctctcg 
      ggggtggtg tttgacggg gaggaaatg cactacgtt 
      gagaataaa gacggggat ttgcactac ttgtacaag 
      catgcggac ctggtggag gggaacaag acatgtggg 
      tatgcgggg gacgcagtg gacccccag aagagccac 
      aatcacaac aggatactt aggtataag agagccgcg 
      gagcagaac agtgccgtg aggggtccc tacaacgct 
      aacaagaag tcgaaatac gtcgaattg gttctagtt 
      gtggacaac cgcgagtac aaagaactg ggtgagagc 
      acaagtaaa gtggaacac cattgcaaa accattgcc 
      aacataata aacgggctg tacgcccct cttaacata 
      ttcatagct ttagtcggt gtggtcatt tggacggaa 
      cacgacgaa atcagtttt tcccccaac ggcgacacc 
      accctcacc agcttcctc cactaccga cgcaacaaa 
      ctagtcaag gagcacccc aacgacaac gcccaactc 
      ttaacgaaa tacaatttc gacaacggc gtcgtgggg 
      aaggccctc aaaggcccg atttgtact tatgagtac 
      tcggggggc gtcaacacg gaccactcc cccgtcgtg 
      gggctcgtc gccaccaca gtggcccac gaaatgggg 
      cacaatttc ggcatggag cacgacacg aatgagtgc 
      acgtgtccg gacgaccgg tgcattatg gcccccagt 
      agctcgaca gtggcgcca acacattgg tcctcctgc 
      agcctcaac tacctccta cttgcattc acacacggc 
      atggactac tgtctcaag aacaaaccg acggcgctg 
      tttgatagc cccgtctgt ggcaacggt ttcgtcgaa 
      ccgggggaa cagtgcgat tgtgggctt ccggagcac 
      tgtgacaac acttgttgc aatgccacc acgtgtatg 
      ttgcacacg aatgcgagc tgtgcgacg ggagaatgc 
      tgcgattta acaacttgt aaaccgaaa tcggcgggt 
      acgttgtgt cgatcggcc gattacgaa tgcgatttg 
      cccgaatat tgcaccggt catagtgaa tattgcccg 
      gctgatatc tacaaaatg gatgctgaa atgtgtgat 
      ggcgggaag gcgttttgc tatcatggc ttttgcagg 
      actaggact gatcagtgt aaacttttg tggggcgaa 
      acgggcaaa tccagtgac gaccagtgt tacaaaatg 
      aatattaaa ggaaatcga catggaaat tgcggttat 
      gataagttc aacaagagt ttcttcaag tgcaacgat 
      gagaacgtc ttgtgcgga atgcttcat tgcaagcat 
      ttgaacgag cgtttggaa ttcggcatg gaatcggtg 
      tctattttg agccacagt ttcctaaac ttgaaaggt 
      tcaatagtg gcctgtcgg actgccatc gtcgacttg 
      ggcatcaac caagtggat ccaggtctc gcaccagat 
      ggcgccaaa tgtggtgag ggcaagatg tgcgtgaac 
      cagaagtgt atgtccgtg gctagtctc caactagct 
      ggaaaaact tgccctcac aattgcaac aacaacggt 
      tggtgcaac aatttgggc cactgccac tgtaaagat 
      ggctacgct ccgccattt tgcgaggat ccgggacct 
      gggggatcg gaggacagc ggcccagct tcggatcct 
      gacgctcgc aaagagttt gttgtggct ctttttgtg 
      atattcttg gggattatt cccggtttg gtactgatc 
      gcgttcctt acttattat atcaaacac aacgttctg 
      cacattcgt tcgaaatca gcggcccca acgtacgta 
      aaaaattgt ctgtttaac agtttccag catgttgtg 
      tctgcatgt cgtggtggt agtctcttt tccaactca 
      attcccaca gtgtccgaa cgtgttcaa aacattcgt 
      aacttggag atcaagcag acgggtttg atctccaca 
      acaaacact gacgctatt attagaagt aacaccata 
      acaaacggt actaatccg gaaagtggc ggcacacga 
      cgcttatct ttgcgcaat atcaaagga cttaaactc 
      aacacgaat ctacggagg aaagataga tctagtcaa 
      atcgaaacg ccggtgacc actgaaact ctctgtgac 
      acgccgcat atggtggtc agtgtcaag gatctcgcg 
      aaaaaattc aacgatact tcgccgaaa gtgtaa
    mRNA sequence
    • >TC010657 RA
      gctcgcaaa gcgatatag acgtgttgt tgtgcttct 
      tttgaaaga agtcggtct tttgtgcag tatgtgacc 
      agttgttca gtgagaata tggcggatt tagtgtact 
      attatgttt gctttgtgt tgttgttgt tgttatcct 
      tgcagcagg atgtagctg gatccactg aaagaactg 
      aacaacatc ctgccagag acttcagcc gccatacgc 
      tcgtcaagc ctcggattt accacggac gggagaaac 
      gtcagatct cgacaagca aagacgaga atggaccat 
      atcacctcg accacttga ccatatcac tgccaatcg 
      acggcgaag acttcctcc tagacttga ccctgaatc 
      gacagctca ttccgaaag gattcttcc gcaaggttc 
      agcaaaacg gaacgcacc aagtcatac gacccagac 
      cggaggagg tcgacctgt gccactatg ggggcaaag 
      tcaggaaca aaccgggct cgtgggtcg ccctgtcca 
      cctgcgacg ggctctcgg gggtggtgt ttgacgggg 
      aggaaatgc actacgttg agaataaag acggggatt 
      tgcactact tgtacaagc atgcggacc tggtggagg 
      ggaacaaga catgtgggt atgcggggg acgcagtgg 
      acccccaga agagccaca atcacaaca ggatactta 
      ggtataaga gagccgcgg agcagaaca gtgccgtga 
      ggggtccct acaacgcta acaagaagt cgaaatacg 
      tcgaattgg ttctagttg tggacaacc gcgagtaca 
      aagaactgg gtgagagca caagtaaag tggaacacc 
      attgcaaaa ccattgcca acataataa acgggctgt 
      acgcccctc ttaacatat tcatagctt tagtcggtg 
      tggtcattt ggacggaac acgacgaaa tcagttttt 
      cccccaacg gcgacacca ccctcacca gcttcctcc 
      actaccgac gcaacaaac tagtcaagg agcacccca 
      acgacaacg cccaactct taacgaaat acaatttcg 
      acaacggcg tcgtgggga aggccctca aaggcccga 
      tttgtactt atgagtact cggggggcg tcaacacgg 
      accactccc ccgtcgtgg ggctcgtcg ccaccacag 
      tggcccacg aaatggggc acaatttcg gcatggagc 
      acgacacga atgagtgca cgtgtccgg acgaccggt 
      gcattatgg cccccagta gctcgacag tggcgccaa 
      cacattggt cctcctgca gcctcaact acctcctac 
      ttgcattca cacacggca tggactact gtctcaaga 
      acaaaccga cggcgctgt ttgatagcc ccgtctgtg 
      gcaacggtt tcgtcgaac cgggggaac agtgcgatt 
      gtgggcttc cggagcact gtgacaaca cttgttgca 
      atgccacca cgtgtatgt tgcacacga atgcgagct 
      gtgcgacgg gagaatgct gcgatttaa caacttgta 
      aaccgaaat cggcgggta cgttgtgtc gatcggccg 
      attacgaat gcgatttgc ccgaatatt gcaccggtc 
      atagtgaat attgcccgg ctgatatct acaaaatgg 
      atgctgaaa tgtgtgatg gcgggaagg cgttttgct 
      atcatggct tttgcagga ctaggactg atcagtgta 
      aacttttgt ggggcgaaa cgggcaaat ccagtgacg 
      accagtgtt acaaaatga atattaaag gaaatcgac 
      atggaaatt gcggttatg ataagttca acaagagtt 
      tcttcaagt gcaacgatg agaacgtct tgtgcggaa 
      tgcttcatt gcaagcatt tgaacgagc gtttggaat 
      tcggcatgg aatcggtgt ctattttga gccacagtt 
      tcctaaact tgaaaggtt caatagtgg cctgtcgga 
      ctgccatcg tcgacttgg gcatcaacc aagtggatc 
      caggtctcg caccagatg gcgccaaat gtggtgagg 
      gcaagatgt gcgtgaacc agaagtgta tgtccgtgg 
      ctagtctcc aactagctg gaaaaactt gccctcaca 
      attgcaaca acaacggtt ggtgcaaca atttgggcc 
      actgccact gtaaagatg gctacgctc cgccatttt 
      gcgaggatc cgggacctg ggggatcgg aggacagcg 
      gcccagctt cggatcctg acgctcgca aagagtttg 
      ttgtggctc tttttgtga tattcttgg ggattattc 
      ccggtttgg tactgatcg cgttcctta cttattata 
      tcaaacaca acgttctgc acattcgtt cgaaatcag 
      cggccccaa cgaccaagt cgcctgcga aaacacgca 
      ccgccccgt tcggccccg attccacca aacggaccg 
      aagacaacc actccctcc tgcaaaacg acccgccca 
      cttcgcccc tacagtgcg agtttttcg gctatttta 
      aaggtttct ccataacac cattaaaga aagtcgaaa 
      atcagccag caagggcgg cacccccgc ccccgatca 
      tacccccat cggttccac cgccaccta aaaacaatt 
      ttttgacac gctccatgt ccagatctc actcattta 
      agcaagctt tggccggcg tctctcaga atgtgagtg 
      cgccgacgc taccgcccc ctaaccccg gcagcacgg 
      ctaaaccaa tcatatcgt cgccgattt tggagaatt 
      caacttgca cggccaaag aattaatct cgccgttga 
      ggaacgccc ccaaagtgc cggtaaggc ccgcccccg 
      aggccccgc ccacaaaag atggcaata cgctaaata 
      gaataactt cgttcttga agaaaacta ctcctgctg 
      gcagtacta ctagtagta gcactagta ctagtactt 
      tgcccagaa aggcgagta aaattatgg ataagaacg 
      cactgagat cgattgaaa tttcaaatc cgattctac 
      aacccgaag gcaccacag ttagtgttg gaggggagg 
      gtaataagg cggtgataa tgagggcgc aaagtatga 
      gaggcacta gtgtgaccc ctagaccta atatacaaa 
      ctttcgggt caatgagac aaccaggag gtgccaggc 
      gccccttga gcatacctt cagggagtc gccctaaga 
      gccccccgc ctccggccc cgcccactt ctgacaaaa 
      aattacaaa tacccagcc tgcctggat accaaaaac 
      caacagtca aaacgagcc aaaaccaat atgacgatt 
      gtttaaaca aaacagcac ctttagcaa aactaaccg 
      aagaactaa gcagtgata acatttacg cagtgattg 
      aagaatcac cagtcacgc cccctgaag acaagccca 
      agaccagtt caagttcga acgaaagca tgggcctcc 
      tcggagaaa tagtctcag aaatccaaa atcgcaatt 
      ttgactcaa tttactcaa cttcgaccc tcgcccgca 
      agaagaaac aagaagagg aagcaaaaa tcaaaagcc 
      ccgaatcgg acacgtacg tcaacaccc cgtacaaaa 
      ccccggaaa gcgtctaca gcaacatga ctagctcca 
      cctccagcg gctacatcc tcccatcgg cggtcaacc 
      cgccaatca aatcgcccg aggaaccca aactctcca 
      ccttcaaag cgaagtcgc gcagcccca ccccgacca 
      agcgcgcgc ccaataaaa ccgcgatca atcgccaga 
      ccacgcccc cgaatctgc gcacgcgca agccgtcgc 
      cggttaggc ccgcccccg gggccccga agccacgcc 
      cagcgccca acagcccgg atgtcgtga cgagttgca 
      acaccaaac ccgggaata agggaccgg atgttgtca 
      atacgaaga aaccgacga ttgcgacca ccaaaccga 
      atgttaatg gattgccca gaggggcgg ttaaaccga 
      aagttagtt tcaataaac cgggggaga ggaaggtgg 
      ctacggtta gtaagagcc aatcggatg cgaagcctg 
      tgccgtcag gggtcagga acgcggcga ggtcgtcgt 
      caaatgtgg cgtcgctgc agcagaaat tcgagacta 
      agactttaa acgcgaaaa gtaacaagg cgtgatgat 
      tgtgataaa attgtgtca aaaaggcct taaaggaca 
      atcagtctg tcgagactt gtatataac ttgatgagt 
      aatttatta ttattgatc atggcggct gttggacga 
      actttgatg tacgcgtgg ttttgggct aacttagca 
      cagttggtt cgaaaacca gcgattttt gcctacttt 
      tatcgtggc tttaaaact agtctttct gcatcacag 
      tagcgttta ccaagtctt tgcttgctc aagttggca 
      gctcaaact cgtatttta cagggacct gtttgtccg 
      atagccggc tctcgtcga ttttgttat tattaagta 
      ttcctcttg cgaataatg tgatttttt tcacattct 
      tactttatt atacacctt ttaggttcg taattttgt 
      atataatac caaagatta ttataattg tactcaaat 
      tcttgtaaa ataaacgac tatttctcc a
    • >TC010657 RB
      gctcgcaaa gcgatatag acgtgttgt tgtgcttct 
      tttgaaaga agtcggtct tttgtgcag tatgtgacc 
      agttgttca gtgagaata tggcggatt tagtgtact 
      attatgttt gctttgtgt tgttgttgt tgttatcct 
      tgcagcagg atgtagctg gatccactg aaagaactg 
      aacaacatc ctgccagag acttcagcc gccatacgc 
      tcgtcaagc ctcggattt accacggac gggagaaac 
      gtcagatct cgacaagca aagacgaga atggaccat 
      atcacctcg accacttga ccatatcac tgccaatcg 
      acggcgaag acttcctcc tagacttga ccctgaatc 
      gacagctca ttccgaaag gattcttcc gcaaggttc 
      agcaaaacg gaacgcacc aagtcatac gacccagac 
      cggaggagg tcgacctgt gccactatg ggggcaaag 
      tcaggaaca aaccgggct cgtgggtcg ccctgtcca 
      cctgcgacg ggctctcgg gggtggtgt ttgacgggg 
      aggaaatgc actacgttg agaataaag acggggatt 
      tgcactact tgtacaagc atgcggacc tggtggagg 
      ggaacaaga catgtgggt atgcggggg acgcagtgg 
      acccccaga agagccaca atcacaaca ggatactta 
      ggtataaga gagccgcgg agcagaaca gtgccgtga 
      ggggtccct acaacgcta acaagaagt cgaaatacg 
      tcgaattgg ttctagttg tggacaacc gcgagtaca 
      aagaactgg gtgagagca caagtaaag tggaacacc 
      attgcaaaa ccattgcca acataataa acgggctgt 
      acgcccctc ttaacatat tcatagctt tagtcggtg 
      tggtcattt ggacggaac acgacgaaa tcagttttt 
      cccccaacg gcgacacca ccctcacca gcttcctcc 
      actaccgac gcaacaaac tagtcaagg agcacccca 
      acgacaacg cccaactct taacgaaat acaatttcg 
      acaacggcg tcgtgggga aggccctca aaggcccga 
      tttgtactt atgagtact cggggggcg tcaacacgg 
      accactccc ccgtcgtgg ggctcgtcg ccaccacag 
      tggcccacg aaatggggc acaatttcg gcatggagc 
      acgacacga atgagtgca cgtgtccgg acgaccggt 
      gcattatgg cccccagta gctcgacag tggcgccaa 
      cacattggt cctcctgca gcctcaact acctcctac 
      ttgcattca cacacggca tggactact gtctcaaga 
      acaaaccga cggcgctgt ttgatagcc ccgtctgtg 
      gcaacggtt tcgtcgaac cgggggaac agtgcgatt 
      gtgggcttc cggagcact gtgacaaca cttgttgca 
      atgccacca cgtgtatgt tgcacacga atgcgagct 
      gtgcgacgg gagaatgct gcgatttaa caacttgta 
      aaccgaaat cggcgggta cgttgtgtc gatcggccg 
      attacgaat gcgatttgc ccgaatatt gcaccggtc 
      atagtgaat attgcccgg ctgatatct acaaaatgg 
      atgctgaaa tgtgtgatg gcgggaagg cgttttgct 
      atcatggct tttgcagga ctaggactg atcagtgta 
      aacttttgt ggggcgaaa cgggcaaat ccagtgacg 
      accagtgtt acaaaatga atattaaag gaaatcgac 
      atggaaatt gcggttatg ataagttca acaagagtt 
      tcttcaagt gcaacgatg agaacgtct tgtgcggaa 
      tgcttcatt gcaagcatt tgaacgagc gtttggaat 
      tcggcatgg aatcggtgt ctattttga gccacagtt 
      tcctaaact tgaaaggtt caatagtgg cctgtcgga 
      ctgccatcg tcgacttgg gcatcaacc aagtggatc 
      caggtctcg caccagatg gcgccaaat gtggtgagg 
      gcaagatgt gcgtgaacc agaagtgta tgtccgtgg 
      ctagtctcc aactagctg gaaaaactt gccctcaca 
      attgcaaca acaacggtt ggtgcaaca atttgggcc 
      actgccact gtaaagatg gctacgctc cgccatttt 
      gcgaggatc cgggacctg ggggatcgg aggacagcg 
      gcccagctt cggatcctg acgctcgca aagagtttg 
      ttgtggctc tttttgtga tattcttgg ggattattc 
      ccggtttgg tactgatcg cgttcctta cttattata 
      tcaaacaca acgttctgc acattcgtt cgaaatcag 
      cggccccaa cgtacgtaa aaaattgtc tgtttaaca 
      gtttccagc atgttgtgt ctgcatgtc gtggtggta 
      gtctctttt ccaactcaa ttcccacag tgtccgaac 
      gtgttcaaa acattcgta acttggaga tcaagcaga 
      cgggtttga tctccacaa caaacactg acgctatta 
      ttagaagta acaccataa caaacggta ctaatccgg 
      aaagtggcg gcacacgac gcttatctt tgcgcaata 
      tcaaaggac ttaaactca acacgaatc tacggagga 
      aagatagat ctagtcaaa tcgaaacgc cggtgacca 
      ctgaaactc tctgtgaca cgccgcata tggtggtca 
      gtgtcaagg atctcgcga aaaaattca acgatactt 
      cgccgaaag tgtaacgtt tttattttg accgacttt 
      cgaattgtt acaagttcg cacgttgtg taacaggtg 
      aagtggaac catgcgtac ttgtaacaa tttgataaa 
      gtagcacag agttttgtt acgccagtg tacaccgcc 
      tggggactt taaggctaa aggggatgg cagagtgtg 
      aaatgaaaa