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Gene information
General information
TC010647 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid67: 382568..388124
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC010647
      iBeetle screen iB_01721
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (16 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC010647 RA
        MTDCQIQAF LFLVLIILG NAEDLDEGN GNRNKFNVV 
        LFLAKTNTC ETYSQDSGI RFLQFVETK RVKHCATGI 
        TEVQPVESC TSQGNDCEE LLKTGIYSL ISFCRNTMG 
        MTTAVASVD NDTFSDGLR FRKSTSEDL EIRFHHPRN 
        IRSRLGGSP NQKNRPESH LITQPEHTE VFKIRSKRE 
        FHIDNNNNQ GMTPVDEND PNFDIFGIM ANIKLTFFG 
        KLFDQDTPA NKKPIAQLQ NNVMNQIGK VTENSLFDL 
        VKETLNNVQ DCRNGFLMI VIGGCLKDV SDIYSPLMQ 
        SVKHIVENT DPQNTLIVL TGQCPGGEN NNQQFTIPV 
        YTRGPPKCS GIAVAEQLY DVPYAIKNM LTENYGGSF 
        FPSVKSKRS KRNIDEKRN SAPPTHPLF LFPLFLALL 
        TTVYK
      CDS sequence
      • >TC010647 RA
        atgacagat tgtcaaatc caagctttt ttgttttta 
        gttttaata attttgggg aacgcagaa gatttggat 
        gaaggaaac ggtaacaga aacaagttc aatgtggtt 
        ctatttttg gccaaaact aatacctgt gagacttac 
        agtcaagac agtggaatt cgattttta caatttgtt 
        gaaactaaa cgagtgaaa cactgtgcc accggtatc 
        accgaagtc cagccagtg gagagctgc acttcgcag 
        ggaaatgac tgtgaagag ttgctcaaa acgggaatt 
        tattccttg attagtttt tgccgaaac accatgggc 
        atgaccacg gcagtggcg tcggtcgat aatgacacc 
        ttcagtgat ggtctcaga ttcagaaaa tcaacttca 
        gaggatttg gaaatacga tttcaccac cctagaaat 
        atccgaagt cgccttggt ggttcccca aaccaaaaa 
        aaccgaccg gaatcacac ttaatcact caaccggaa 
        catacagag gtgttcaaa atccgatcg aaacgagag 
        tttcacatc gataataat aacaatcaa gggatgaca 
        ccggtggat gaaaatgac ccaaacttt gacattttt 
        gggattatg gccaatatt aaattaacc ttctttgga 
        aaattattc gatcaagac acacccgct aacaaaaaa 
        ccgatcgca cagctgcag aataacgtc atgaaccaa 
        ataggaaaa gtcaccgaa aattccctc tttgatttg 
        gtcaaagaa acactgaac aacgtccag gactgtcga 
        aatgggttc ctcatgatt gtgatagga ggttgcttg 
        aaggacgtt tctgacatc tattcccct ctaatgcaa 
        agtgtgaaa cacattgtc gagaacact gacccacaa 
        aacactctt attgtcctg actggacaa tgtccaggc 
        ggtgaaaat aataaccaa cagttcacg attcctgtt 
        tataccaga ggtcctccg aaatgtagc ggaatcgcg 
        gttgcggaa caactttat gacgtgccc tacgctata 
        aaaaatatg ttaaccgag aattacgga ggctcgttc 
        ttcccaagc gtcaaaagc aaaagaagc aaacgcaac 
        attgacgaa aaacgaaac agtgcgcca cccactcat 
        cccttattc ctgtttccc cttttcctt gcacttctc 
        acaactgta tacaaataa
      mRNA sequence
      • >TC010647 RA
        acggccata aataaaatg acagattgt caaatccaa 
        gcttttttg tttttagtt ttaataatt ttggggaac 
        gcagaagat ttggatgaa ggaaacggt aacagaaac 
        aagttcaat gtggttcta tttttggcc aaaactaat 
        acctgtgag acttacagt caagacagt ggaattcga 
        tttttacaa tttgttgaa actaaacga gtgaaacac 
        tgtgccacc ggtatcacc gaagtccag ccagtggag 
        agctgcact tcgcaggga aatgactgt gaagagttg 
        ctcaaaacg ggaatttat tccttgatt agtttttgc 
        cgaaacacc atgggcatg accacggca gtggcgtcg 
        gtcgataat gacaccttc agtgatggt ctcagattc 
        agaaaatca acttcagag gatttggaa atacgattt 
        caccaccct agaaatatc cgaagtcgc cttggtggt 
        tccccaaac caaaaaaac cgaccggaa tcacactta 
        atcactcaa ccggaacat acagaggtg ttcaaaatc 
        cgatcgaaa cgagagttt cacatcgat aataataac 
        aatcaaggg atgacaccg gtggatgaa aatgaccca 
        aactttgac atttttggg attatggcc aatattaaa 
        ttaaccttc tttggaaaa ttattcgat caagacaca 
        cccgctaac aaaaaaccg atcgcacag ctgcagaat 
        aacgtcatg aaccaaata ggaaaagtc accgaaaat 
        tccctcttt gatttggtc aaagaaaca ctgaacaac 
        gtccaggac tgtcgaaat gggttcctc atgattgtg 
        ataggaggt tgcttgaag gacgtttct gacatctat 
        tcccctcta atgcaaagt gtgaaacac attgtcgag 
        aacactgac ccacaaaac actcttatt gtcctgact 
        ggacaatgt ccaggcggt gaaaataat aaccaacag 
        ttcacgatt cctgtttat accagaggt cctccgaaa 
        tgtagcgga atcgcggtt gcggaacaa ctttatgac 
        gtgccctac gctataaaa aatatgtta accgagaat 
        tacggaggc tcgttcttc ccaagcgtc aaaagcaaa 
        agaagcaaa cgcaacatt gacgaaaaa cgaaacagt 
        gcgccaccc actcatccc ttattcctg tttcccctt 
        ttccttgca cttctcaca actgtatac aaataaact 
        ccccccagc cactcctta tacttactg gatgcatgg 
        gcatcctga acggcctcg gtcccggag gcggcgaga 
        atccgggcg gcacgggcg acatcgccg tgggccacc 
        gcggtctcg gacacggca tgtaaggca accgattgc 
        cgaacatgt tcattccgg ggctcccca gcctcgggg 
        gcggcgact gggtgtagg gagacggcg gccggaagt 
        gatgcggcg acctgccgc cgcctccgc ggccccctt 
        gcccagaca tcgcgcact ttcacaaac gatgatcgg 
        tcattggat ctacgaaac acagcggtt ttttcgacg 
        gataaatat cgaagttat cgcaggtgc gatcgggag 
        aattgtctt gtccctgag gccgtgttg tggaattaa 
        ggtcagaga cgtcctatt tttaggatt cgccgagtt 
        tattcatgc gagtagcaa atggagcga aaatagagc 
        gattgtcga ggcatgggg ttgggggga ggttcgacc 
        actggaggt aaatatttg ggagtgagt caggtttgt 
        tttttcggg ctggatatt aataggtga ggcaatggg 
        tcgcaggat ttaaggctg atggatgga agtcggaca 
        ggtctttgt ggatttacg acaagtcgc tattaaatt 
        tatctacta tcc