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Gene information
General information
TC010609 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451776.1: 6694..24023
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010609
    iBeetle screen iB_01717

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    previtellogenic egg chamber not present - Number of animals showing the phenotype: 4

    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection

    Fertility
    egg production: number no/almost no eggs -
    ovariole shape rounded - Number of animals showing the phenotype: 5

    egg chamber irregular - Number of animals showing the phenotype: 5

    Comment: No individual egg chamber present!
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010609 RA
      MDVTRNPYA EFDALLADL QNTVSPGGG SSSGYGSLN 
ASRDGRQSG AESPASRVD SYGVRSGTY GKTQPTSGA 
RTPGYGYGS GPGAGEKRT FNNNLSELD TLLQDLSNS 
RYGNISENG MNGTLNGST YNGSGGLSP HSRPSSAQS 
TLNRRTVDS LLEELNPGE VIYAGPKKV TITVKETKT 
ETGYPGETE LISSHISTS TAPAASTAT RELDDLMAS 
LSGIKVNQG QPQHQTVTD SQYARPTKA TQSHSAPKQ 
NLDSMLGNL QADMSRQGV NTSQKGCCS ACDKPIVGQ 
VITALGKTW HPEHFTCAH CTQELGTRN FFERDGKPY 
CEPDYHNLF SPRCAYCNG PILDKCVTA LEKTWHMEH 
FFCAQCGKQ FGEEGFHER EGKPYCRDD YFDMFAPKC 
GACNRAIME NYISALNAQ WHPDCFVCR DCKLPVQGK 
SFYAVEGKP VCPACIGRE DEEDE
    CDS sequence
    • >TC010609 RA
      atggacgtg actcgaaat ccgtacgcc gaattcgat 
gctctcttg gccgatctt caaaacacc gtgtctccg 
ggtggcggg agcagctcc ggttacggg tccttgaat 
gcgtccagg gatggacga caatcgggg gctgaaagt 
ccagcctca agagtcgat tcgtatggt gttagatcg 
ggtacttat ggcaagacc caaccgact tctggagcc 
cgcactccg gggtacggg tacggttcc gggcccgga 
gccggcgag aaacgcact tttaataac aatctgtcc 
gagctcgat acgctcttg caggacttg agcaattcg 
cgttatggc aacatttcc gaaaatgga atgaatgga 
actttgaac ggaagtact tacaacggc tcgggcggc 
ttgagcccc cattcgcgt ccctcgagc gcccaaagt 
actttgaac agacgcaca gtcgatagt cttttggag 
gaattaaat cccggtgaa gtgatttat gctggtccg 
aaaaaagtg accatcact gtgaaagaa acaaaaacc 
gagacgggt tatcccggt gaaaccgaa ctgatttca 
tcccacatc agcacgtct acggcccct gcagcctca 
acggccacc cgagagctc gacgatttg atggcgagt 
ctgtccggc atcaaggtg aaccagggc cagccccag 
caccagacg gtcaccgac tcgcagtac gcgcgcccc 
acgaaagcg acgcagtcc cacagcgcc cccaagcag 
aacttggac tctatgctc ggcaacctc caggcggac 
atgagccga caaggggtc aacaccagc cagaagggc 
tgctgcagc gcctgcgac aagcccatc gtcgggcag 
gtcatcacc gcgctgggc aagacctgg cacccggag 
cacttcacc tgcgcccac tgcacccag gagctgggc 
acgcggaac ttcttcgag cgcgacggg aagccctat 
tgcgagccg gactaccac aaccttttc agtccgcgc 
tgcgcctac tgcaacggg cctattttg gataaatgc 
gtgaccgcg ctggaaaag acgtggcac atggaacac 
ttcttctgc gcgcaatgc ggcaagcag ttcggggaa 
gaagggttc catgagcgc gaagggaag ccctactgt 
agggacgat tacttcgac atgttcgcc cccaagtgc 
ggggcgtgc aatagggcc atcatggag aactacatc 
tcggcgctg aacgcgcaa tggcacccc gattgcttt 
gtgtgcagg gattgcaag ttgccggtg caagggaag 
tcgttttat gcggttgaa ggcaaaccg gtgtgtcca 
gcatgcatc gggcgggag gacgaagaa gacgaataa
    mRNA sequence
    • >TC010609 RA
      tttgagaat ttttccgta aaagacaca cttgtttgt 
ctcgaaaaa cgttctgaa gcgagcgaa atcggcgaa 
aatagcaag tttgaagcg tattttttc tgattagag 
aaacaacat ttgtgtgga tattgagtt tgttaatga 
aaaatgctt taaaaaact aaaaaatag gccaattgg 
gatgatcat tttcgtata ttctttagg aaaatacgt 
cgaaattga cgaaggtat aattcttgt cttgtttgg 
taaaattct ttttgaaaa acattgcaa atcgctcgg 
aaataacga attctagat ctaattatg cgattgttt 
tttgggtga aaaactcgt tagaaattt ataaaaaat 
agagaataa taatatttg cagctatat tttgcgact 
tctgacttg aatttcttg gtaaaaagg attctaatc 
aatttagca aaacagggg aaagcatcg atggttgga 
ctaatttgt gaatttttc gccgaaaaa cggttcgag 
ttaacagaa acagatata attttgcga tttcagctt 
tgtttttac aaaacctaa aattgagga attattgag 
tttctgtca aaagccatc tagaaacga tattttctg 
gtacgtgac aattttttg tgtgaagtc ttagagtcg 
agttatcaa gaactttta aaaacaagc caaaccacc 
gagcaactt gagtcattt ccacagtat tgttaggaa 
aatgatttc atgttgtcg caattttga ttaaatttg 
tattcaaga gagtgctaa ataaaggaa tgaaatgtg 
tggcattta ttttttgat ttcacctgt ttttatttc 
caattatgt tttccaagt acgcaatgt ctgtaaaag 
tccacggtc ataaccggc gcgtaggtt taacaatcg 
taggttttt tcaataaaa agttgtaac tcgcacgta 
tttgaatcc ttggacgat tttctaaat cagtcgatc 
gtggagaca cacgcatcg tctaaattt acctcagtc 
ttagcgttg acgtttccg acaaatcgt ttccggaaa 
atttcgcga aaatgccac ttacgtaag gtggaaaag 
gtggagttt tcgtgattc cgcaagtgg tctatttat 
aaccggaca cgtccaggt ttgccaaaa ctcgcgggt 
gcaacagtt agtcatagt cgggatctt cccaaaact 
attgttttt ttctcgctt cgtgttgga aaaacgaaa 
tgagtgcta aattgcgca cgcggacgc cctcaccca 
aaacggtca ctttcgacc cctcgcctc cgacgacac 
tggagcgga agcggaaag tgatgaggt ttaaatcgc 
acgccgaat gggagcaat atttaatta attgcagcc 
caaggatgt gtctgcatt gctgttctt actaatcag 
tgccaccac cgtgtttta ttaataacc gtattccgt 
gtgataaca ccgttttcc ccaaattgt gacgcatcc 
tttatcagc ttggcggtt ttaatttcg agtaattga 
ctcattttg tccaatttc tttcaatca ccaaccgat 
ttattaatt atactaagc tcggacgaa atggacgtg 
actcgaaat ccgtacgcc gaattcgat gctctcttg 
gccgatctt caaaacacc gtgtctccg ggtggcggg 
agcagctcc ggttacggg tccttgaat gcgtccagg 
gatggacga caatcgggg gctgaaagt ccagcctca 
agagtcgat tcgtatggt gttagatcg ggtacttat 
ggcaagacc caaccgact tctggagcc cgcactccg 
gggtacggg tacggttcc gggcccgga gccggcgag 
aaacgcact tttaataac aatctgtcc gagctcgat 
acgctcttg caggacttg agcaattcg cgttatggc 
aacatttcc gaaaatgga atgaatgga actttgaac 
ggaagtact tacaacggc tcgggcggc ttgagcccc 
cattcgcgt ccctcgagc gcccaaagt actttgaac 
agacgcaca gtcgatagt cttttggag gaattaaat 
cccggtgaa gtgatttat gctggtccg aaaaaagtg 
accatcact gtgaaagaa acaaaaacc gagacgggt 
tatcccggt gaaaccgaa ctgatttca tcccacatc 
agcacgtct acggcccct gcagcctca acggccacc 
cgagagctc gacgatttg atggcgagt ctgtccggc 
atcaaggtg aaccagggc cagccccag caccagacg 
gtcaccgac tcgcagtac gcgcgcccc acgaaagcg 
acgcagtcc cacagcgcc cccaagcag aacttggac 
tctatgctc ggcaacctc caggcggac atgagccga 
caaggggtc aacaccagc cagaagggc tgctgcagc 
gcctgcgac aagcccatc gtcgggcag gtcatcacc 
gcgctgggc aagacctgg cacccggag cacttcacc 
tgcgcccac tgcacccag gagctgggc acgcggaac 
ttcttcgag cgcgacggg aagccctat tgcgagccg 
gactaccac aaccttttc agtccgcgc tgcgcctac 
tgcaacggg cctattttg gataaatgc gtgaccgcg 
ctggaaaag acgtggcac atggaacac ttcttctgc 
gcgcaatgc ggcaagcag ttcggggaa gaagggttc 
catgagcgc gaagggaag ccctactgt agggacgat 
tacttcgac atgttcgcc cccaagtgc ggggcgtgc 
aatagggcc atcatggag aactacatc tcggcgctg 
aacgcgcaa tggcacccc gattgcttt gtgtgcagg 
gattgcaag ttgccggtg caagggaag tcgttttat 
gcggttgaa ggcaaaccg gtgtgtcca gcatgcatc 
gggcgggag gacgaagaa gacgaataa atatgctag 
ccaccccta ataaagtct aaccctgtc ccgtaaata 
agagcaaag tcttgtata tttgtatta tattgagat 
aataaagat ttaataaaa aagaaaaca caaaaatac 
acacacaaa tagaatcag ggcgatttt ctgcgcgct 
cagagcgct gcgttgtcg cccaggtga ggcctagcg 
gccgtttta taaattgtg tgtttattg ggtcagttg 
ctacgtctt tgtacatca tatttaagc taggattat 
ttataggtc gggtaccta cacgaaaat gtaggacaa 
atttatgaa acttgtctc tgtctcgtt ctatttaat 
gggttttgt tgcaccacg tttctggcc ttgatttgt 
agcaactag acgagccaa aatcattgt aatttttct 
ctgcgttat ttatttcat tcgatttgc taatttcgt 
ttcgattta atggcgacg ggaaacaag acagctcaa 
ttaatggca ggcgtaatc aatttttat tattgttct 
gtggtgacg gaaagataa attaatttg agaaagtgg 
gaagaaatt agatgaaca tgt
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