Status
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Gene information
General information
TC010609 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451776.1: 6694..24023
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010609
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01717

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber not present - Number of animals showing the phenotype: 4

    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    ovariole shape rounded - Number of animals showing the phenotype: 5

    egg chamber irregular - Number of animals showing the phenotype: 5

    Comment: No individual egg chamber present!
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010609 RA
      MDVTRNPYA EFDALLADL QNTVSPGGG SSSGYGSLN 
      ASRDGRQSG AESPASRVD SYGVRSGTY GKTQPTSGA 
      RTPGYGYGS GPGAGEKRT FNNNLSELD TLLQDLSNS 
      RYGNISENG MNGTLNGST YNGSGGLSP HSRPSSAQS 
      TLNRRTVDS LLEELNPGE VIYAGPKKV TITVKETKT 
      ETGYPGETE LISSHISTS TAPAASTAT RELDDLMAS 
      LSGIKVNQG QPQHQTVTD SQYARPTKA TQSHSAPKQ 
      NLDSMLGNL QADMSRQGV NTSQKGCCS ACDKPIVGQ 
      VITALGKTW HPEHFTCAH CTQELGTRN FFERDGKPY 
      CEPDYHNLF SPRCAYCNG PILDKCVTA LEKTWHMEH 
      FFCAQCGKQ FGEEGFHER EGKPYCRDD YFDMFAPKC 
      GACNRAIME NYISALNAQ WHPDCFVCR DCKLPVQGK 
      SFYAVEGKP VCPACIGRE DEEDE
    CDS sequence
    • >TC010609 RA
      atggacgtg actcgaaat ccgtacgcc gaattcgat 
      gctctcttg gccgatctt caaaacacc gtgtctccg 
      ggtggcggg agcagctcc ggttacggg tccttgaat 
      gcgtccagg gatggacga caatcgggg gctgaaagt 
      ccagcctca agagtcgat tcgtatggt gttagatcg 
      ggtacttat ggcaagacc caaccgact tctggagcc 
      cgcactccg gggtacggg tacggttcc gggcccgga 
      gccggcgag aaacgcact tttaataac aatctgtcc 
      gagctcgat acgctcttg caggacttg agcaattcg 
      cgttatggc aacatttcc gaaaatgga atgaatgga 
      actttgaac ggaagtact tacaacggc tcgggcggc 
      ttgagcccc cattcgcgt ccctcgagc gcccaaagt 
      actttgaac agacgcaca gtcgatagt cttttggag 
      gaattaaat cccggtgaa gtgatttat gctggtccg 
      aaaaaagtg accatcact gtgaaagaa acaaaaacc 
      gagacgggt tatcccggt gaaaccgaa ctgatttca 
      tcccacatc agcacgtct acggcccct gcagcctca 
      acggccacc cgagagctc gacgatttg atggcgagt 
      ctgtccggc atcaaggtg aaccagggc cagccccag 
      caccagacg gtcaccgac tcgcagtac gcgcgcccc 
      acgaaagcg acgcagtcc cacagcgcc cccaagcag 
      aacttggac tctatgctc ggcaacctc caggcggac 
      atgagccga caaggggtc aacaccagc cagaagggc 
      tgctgcagc gcctgcgac aagcccatc gtcgggcag 
      gtcatcacc gcgctgggc aagacctgg cacccggag 
      cacttcacc tgcgcccac tgcacccag gagctgggc 
      acgcggaac ttcttcgag cgcgacggg aagccctat 
      tgcgagccg gactaccac aaccttttc agtccgcgc 
      tgcgcctac tgcaacggg cctattttg gataaatgc 
      gtgaccgcg ctggaaaag acgtggcac atggaacac 
      ttcttctgc gcgcaatgc ggcaagcag ttcggggaa 
      gaagggttc catgagcgc gaagggaag ccctactgt 
      agggacgat tacttcgac atgttcgcc cccaagtgc 
      ggggcgtgc aatagggcc atcatggag aactacatc 
      tcggcgctg aacgcgcaa tggcacccc gattgcttt 
      gtgtgcagg gattgcaag ttgccggtg caagggaag 
      tcgttttat gcggttgaa ggcaaaccg gtgtgtcca 
      gcatgcatc gggcgggag gacgaagaa gacgaataa
    mRNA sequence
    • >TC010609 RA
      tttgagaat ttttccgta aaagacaca cttgtttgt 
      ctcgaaaaa cgttctgaa gcgagcgaa atcggcgaa 
      aatagcaag tttgaagcg tattttttc tgattagag 
      aaacaacat ttgtgtgga tattgagtt tgttaatga 
      aaaatgctt taaaaaact aaaaaatag gccaattgg 
      gatgatcat tttcgtata ttctttagg aaaatacgt 
      cgaaattga cgaaggtat aattcttgt cttgtttgg 
      taaaattct ttttgaaaa acattgcaa atcgctcgg 
      aaataacga attctagat ctaattatg cgattgttt 
      tttgggtga aaaactcgt tagaaattt ataaaaaat 
      agagaataa taatatttg cagctatat tttgcgact 
      tctgacttg aatttcttg gtaaaaagg attctaatc 
      aatttagca aaacagggg aaagcatcg atggttgga 
      ctaatttgt gaatttttc gccgaaaaa cggttcgag 
      ttaacagaa acagatata attttgcga tttcagctt 
      tgtttttac aaaacctaa aattgagga attattgag 
      tttctgtca aaagccatc tagaaacga tattttctg 
      gtacgtgac aattttttg tgtgaagtc ttagagtcg 
      agttatcaa gaactttta aaaacaagc caaaccacc 
      gagcaactt gagtcattt ccacagtat tgttaggaa 
      aatgatttc atgttgtcg caattttga ttaaatttg 
      tattcaaga gagtgctaa ataaaggaa tgaaatgtg 
      tggcattta ttttttgat ttcacctgt ttttatttc 
      caattatgt tttccaagt acgcaatgt ctgtaaaag 
      tccacggtc ataaccggc gcgtaggtt taacaatcg 
      taggttttt tcaataaaa agttgtaac tcgcacgta 
      tttgaatcc ttggacgat tttctaaat cagtcgatc 
      gtggagaca cacgcatcg tctaaattt acctcagtc 
      ttagcgttg acgtttccg acaaatcgt ttccggaaa 
      atttcgcga aaatgccac ttacgtaag gtggaaaag 
      gtggagttt tcgtgattc cgcaagtgg tctatttat 
      aaccggaca cgtccaggt ttgccaaaa ctcgcgggt 
      gcaacagtt agtcatagt cgggatctt cccaaaact 
      attgttttt ttctcgctt cgtgttgga aaaacgaaa 
      tgagtgcta aattgcgca cgcggacgc cctcaccca 
      aaacggtca ctttcgacc cctcgcctc cgacgacac 
      tggagcgga agcggaaag tgatgaggt ttaaatcgc 
      acgccgaat gggagcaat atttaatta attgcagcc 
      caaggatgt gtctgcatt gctgttctt actaatcag 
      tgccaccac cgtgtttta ttaataacc gtattccgt 
      gtgataaca ccgttttcc ccaaattgt gacgcatcc 
      tttatcagc ttggcggtt ttaatttcg agtaattga 
      ctcattttg tccaatttc tttcaatca ccaaccgat 
      ttattaatt atactaagc tcggacgaa atggacgtg 
      actcgaaat ccgtacgcc gaattcgat gctctcttg 
      gccgatctt caaaacacc gtgtctccg ggtggcggg 
      agcagctcc ggttacggg tccttgaat gcgtccagg 
      gatggacga caatcgggg gctgaaagt ccagcctca 
      agagtcgat tcgtatggt gttagatcg ggtacttat 
      ggcaagacc caaccgact tctggagcc cgcactccg 
      gggtacggg tacggttcc gggcccgga gccggcgag 
      aaacgcact tttaataac aatctgtcc gagctcgat 
      acgctcttg caggacttg agcaattcg cgttatggc 
      aacatttcc gaaaatgga atgaatgga actttgaac 
      ggaagtact tacaacggc tcgggcggc ttgagcccc 
      cattcgcgt ccctcgagc gcccaaagt actttgaac 
      agacgcaca gtcgatagt cttttggag gaattaaat 
      cccggtgaa gtgatttat gctggtccg aaaaaagtg 
      accatcact gtgaaagaa acaaaaacc gagacgggt 
      tatcccggt gaaaccgaa ctgatttca tcccacatc 
      agcacgtct acggcccct gcagcctca acggccacc 
      cgagagctc gacgatttg atggcgagt ctgtccggc 
      atcaaggtg aaccagggc cagccccag caccagacg 
      gtcaccgac tcgcagtac gcgcgcccc acgaaagcg 
      acgcagtcc cacagcgcc cccaagcag aacttggac 
      tctatgctc ggcaacctc caggcggac atgagccga 
      caaggggtc aacaccagc cagaagggc tgctgcagc 
      gcctgcgac aagcccatc gtcgggcag gtcatcacc 
      gcgctgggc aagacctgg cacccggag cacttcacc 
      tgcgcccac tgcacccag gagctgggc acgcggaac 
      ttcttcgag cgcgacggg aagccctat tgcgagccg 
      gactaccac aaccttttc agtccgcgc tgcgcctac 
      tgcaacggg cctattttg gataaatgc gtgaccgcg 
      ctggaaaag acgtggcac atggaacac ttcttctgc 
      gcgcaatgc ggcaagcag ttcggggaa gaagggttc 
      catgagcgc gaagggaag ccctactgt agggacgat 
      tacttcgac atgttcgcc cccaagtgc ggggcgtgc 
      aatagggcc atcatggag aactacatc tcggcgctg 
      aacgcgcaa tggcacccc gattgcttt gtgtgcagg 
      gattgcaag ttgccggtg caagggaag tcgttttat 
      gcggttgaa ggcaaaccg gtgtgtcca gcatgcatc 
      gggcgggag gacgaagaa gacgaataa atatgctag 
      ccaccccta ataaagtct aaccctgtc ccgtaaata 
      agagcaaag tcttgtata tttgtatta tattgagat 
      aataaagat ttaataaaa aagaaaaca caaaaatac 
      acacacaaa tagaatcag ggcgatttt ctgcgcgct 
      cagagcgct gcgttgtcg cccaggtga ggcctagcg 
      gccgtttta taaattgtg tgtttattg ggtcagttg 
      ctacgtctt tgtacatca tatttaagc taggattat 
      ttataggtc gggtaccta cacgaaaat gtaggacaa 
      atttatgaa acttgtctc tgtctcgtt ctatttaat 
      gggttttgt tgcaccacg tttctggcc ttgatttgt 
      agcaactag acgagccaa aatcattgt aatttttct 
      ctgcgttat ttatttcat tcgatttgc taatttcgt 
      ttcgattta atggcgacg ggaaacaag acagctcaa 
      ttaatggca ggcgtaatc aatttttat tattgttct 
      gtggtgacg gaaagataa attaatttg agaaagtgg 
      gaagaaatt agatgaaca tgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY