General information | |||
TC010609 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015451776.1: 6694..24023 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC010609 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01717 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
ovariole shape rounded - Number of animals showing the phenotype: 5
egg chamber irregular - Number of animals showing the phenotype: 5
Comment: No individual egg chamber present! |
- >TC010609 RA
MDVTRNPYA EFDALLADL QNTVSPGGG SSSGYGSLN ASRDGRQSG AESPASRVD SYGVRSGTY GKTQPTSGA RTPGYGYGS GPGAGEKRT FNNNLSELD TLLQDLSNS RYGNISENG MNGTLNGST YNGSGGLSP HSRPSSAQS TLNRRTVDS LLEELNPGE VIYAGPKKV TITVKETKT ETGYPGETE LISSHISTS TAPAASTAT RELDDLMAS LSGIKVNQG QPQHQTVTD SQYARPTKA TQSHSAPKQ NLDSMLGNL QADMSRQGV NTSQKGCCS ACDKPIVGQ VITALGKTW HPEHFTCAH CTQELGTRN FFERDGKPY CEPDYHNLF SPRCAYCNG PILDKCVTA LEKTWHMEH FFCAQCGKQ FGEEGFHER EGKPYCRDD YFDMFAPKC GACNRAIME NYISALNAQ WHPDCFVCR DCKLPVQGK SFYAVEGKP VCPACIGRE DEEDE
- >TC010609 RA
atggacgtg actcgaaat ccgtacgcc gaattcgat gctctcttg gccgatctt caaaacacc gtgtctccg ggtggcggg agcagctcc ggttacggg tccttgaat gcgtccagg gatggacga caatcgggg gctgaaagt ccagcctca agagtcgat tcgtatggt gttagatcg ggtacttat ggcaagacc caaccgact tctggagcc cgcactccg gggtacggg tacggttcc gggcccgga gccggcgag aaacgcact tttaataac aatctgtcc gagctcgat acgctcttg caggacttg agcaattcg cgttatggc aacatttcc gaaaatgga atgaatgga actttgaac ggaagtact tacaacggc tcgggcggc ttgagcccc cattcgcgt ccctcgagc gcccaaagt actttgaac agacgcaca gtcgatagt cttttggag gaattaaat cccggtgaa gtgatttat gctggtccg aaaaaagtg accatcact gtgaaagaa acaaaaacc gagacgggt tatcccggt gaaaccgaa ctgatttca tcccacatc agcacgtct acggcccct gcagcctca acggccacc cgagagctc gacgatttg atggcgagt ctgtccggc atcaaggtg aaccagggc cagccccag caccagacg gtcaccgac tcgcagtac gcgcgcccc acgaaagcg acgcagtcc cacagcgcc cccaagcag aacttggac tctatgctc ggcaacctc caggcggac atgagccga caaggggtc aacaccagc cagaagggc tgctgcagc gcctgcgac aagcccatc gtcgggcag gtcatcacc gcgctgggc aagacctgg cacccggag cacttcacc tgcgcccac tgcacccag gagctgggc acgcggaac ttcttcgag cgcgacggg aagccctat tgcgagccg gactaccac aaccttttc agtccgcgc tgcgcctac tgcaacggg cctattttg gataaatgc gtgaccgcg ctggaaaag acgtggcac atggaacac ttcttctgc gcgcaatgc ggcaagcag ttcggggaa gaagggttc catgagcgc gaagggaag ccctactgt agggacgat tacttcgac atgttcgcc cccaagtgc ggggcgtgc aatagggcc atcatggag aactacatc tcggcgctg aacgcgcaa tggcacccc gattgcttt gtgtgcagg gattgcaag ttgccggtg caagggaag tcgttttat gcggttgaa ggcaaaccg gtgtgtcca gcatgcatc gggcgggag gacgaagaa gacgaataa
- >TC010609 RA
tttgagaat ttttccgta aaagacaca cttgtttgt ctcgaaaaa cgttctgaa gcgagcgaa atcggcgaa aatagcaag tttgaagcg tattttttc tgattagag aaacaacat ttgtgtgga tattgagtt tgttaatga aaaatgctt taaaaaact aaaaaatag gccaattgg gatgatcat tttcgtata ttctttagg aaaatacgt cgaaattga cgaaggtat aattcttgt cttgtttgg taaaattct ttttgaaaa acattgcaa atcgctcgg aaataacga attctagat ctaattatg cgattgttt tttgggtga aaaactcgt tagaaattt ataaaaaat agagaataa taatatttg cagctatat tttgcgact tctgacttg aatttcttg gtaaaaagg attctaatc aatttagca aaacagggg aaagcatcg atggttgga ctaatttgt gaatttttc gccgaaaaa cggttcgag ttaacagaa acagatata attttgcga tttcagctt tgtttttac aaaacctaa aattgagga attattgag tttctgtca aaagccatc tagaaacga tattttctg gtacgtgac aattttttg tgtgaagtc ttagagtcg agttatcaa gaactttta aaaacaagc caaaccacc gagcaactt gagtcattt ccacagtat tgttaggaa aatgatttc atgttgtcg caattttga ttaaatttg tattcaaga gagtgctaa ataaaggaa tgaaatgtg tggcattta ttttttgat ttcacctgt ttttatttc caattatgt tttccaagt acgcaatgt ctgtaaaag tccacggtc ataaccggc gcgtaggtt taacaatcg taggttttt tcaataaaa agttgtaac tcgcacgta tttgaatcc ttggacgat tttctaaat cagtcgatc gtggagaca cacgcatcg tctaaattt acctcagtc ttagcgttg acgtttccg acaaatcgt ttccggaaa atttcgcga aaatgccac ttacgtaag gtggaaaag gtggagttt tcgtgattc cgcaagtgg tctatttat aaccggaca cgtccaggt ttgccaaaa ctcgcgggt gcaacagtt agtcatagt cgggatctt cccaaaact attgttttt ttctcgctt cgtgttgga aaaacgaaa tgagtgcta aattgcgca cgcggacgc cctcaccca aaacggtca ctttcgacc cctcgcctc cgacgacac tggagcgga agcggaaag tgatgaggt ttaaatcgc acgccgaat gggagcaat atttaatta attgcagcc caaggatgt gtctgcatt gctgttctt actaatcag tgccaccac cgtgtttta ttaataacc gtattccgt gtgataaca ccgttttcc ccaaattgt gacgcatcc tttatcagc ttggcggtt ttaatttcg agtaattga ctcattttg tccaatttc tttcaatca ccaaccgat ttattaatt atactaagc tcggacgaa atggacgtg actcgaaat ccgtacgcc gaattcgat gctctcttg gccgatctt caaaacacc gtgtctccg ggtggcggg agcagctcc ggttacggg tccttgaat gcgtccagg gatggacga caatcgggg gctgaaagt ccagcctca agagtcgat tcgtatggt gttagatcg ggtacttat ggcaagacc caaccgact tctggagcc cgcactccg gggtacggg tacggttcc gggcccgga gccggcgag aaacgcact tttaataac aatctgtcc gagctcgat acgctcttg caggacttg agcaattcg cgttatggc aacatttcc gaaaatgga atgaatgga actttgaac ggaagtact tacaacggc tcgggcggc ttgagcccc cattcgcgt ccctcgagc gcccaaagt actttgaac agacgcaca gtcgatagt cttttggag gaattaaat cccggtgaa gtgatttat gctggtccg aaaaaagtg accatcact gtgaaagaa acaaaaacc gagacgggt tatcccggt gaaaccgaa ctgatttca tcccacatc agcacgtct acggcccct gcagcctca acggccacc cgagagctc gacgatttg atggcgagt ctgtccggc atcaaggtg aaccagggc cagccccag caccagacg gtcaccgac tcgcagtac gcgcgcccc acgaaagcg acgcagtcc cacagcgcc cccaagcag aacttggac tctatgctc ggcaacctc caggcggac atgagccga caaggggtc aacaccagc cagaagggc tgctgcagc gcctgcgac aagcccatc gtcgggcag gtcatcacc gcgctgggc aagacctgg cacccggag cacttcacc tgcgcccac tgcacccag gagctgggc acgcggaac ttcttcgag cgcgacggg aagccctat tgcgagccg gactaccac aaccttttc agtccgcgc tgcgcctac tgcaacggg cctattttg gataaatgc gtgaccgcg ctggaaaag acgtggcac atggaacac ttcttctgc gcgcaatgc ggcaagcag ttcggggaa gaagggttc catgagcgc gaagggaag ccctactgt agggacgat tacttcgac atgttcgcc cccaagtgc ggggcgtgc aatagggcc atcatggag aactacatc tcggcgctg aacgcgcaa tggcacccc gattgcttt gtgtgcagg gattgcaag ttgccggtg caagggaag tcgttttat gcggttgaa ggcaaaccg gtgtgtcca gcatgcatc gggcgggag gacgaagaa gacgaataa atatgctag ccaccccta ataaagtct aaccctgtc ccgtaaata agagcaaag tcttgtata tttgtatta tattgagat aataaagat ttaataaaa aagaaaaca caaaaatac acacacaaa tagaatcag ggcgatttt ctgcgcgct cagagcgct gcgttgtcg cccaggtga ggcctagcg gccgtttta taaattgtg tgtttattg ggtcagttg ctacgtctt tgtacatca tatttaagc taggattat ttataggtc gggtaccta cacgaaaat gtaggacaa atttatgaa acttgtctc tgtctcgtt ctatttaat gggttttgt tgcaccacg tttctggcc ttgatttgt agcaactag acgagccaa aatcattgt aatttttct ctgcgttat ttatttcat tcgatttgc taatttcgt ttcgattta atggcgacg ggaaacaag acagctcaa ttaatggca ggcgtaatc aatttttat tattgttct gtggtgacg gaaagataa attaatttg agaaagtgg gaagaaatt agatgaaca tgt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
