Status
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Gene information
General information
TC010593 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 5374997..5380763
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010593
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01715

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010593 RA
      MASTDVLGQ VLSGTLVAK QLTDKLTQD VARLKQRLP 
      GFEPGLAIV QVGGREDSN VYIRMKIKT AGEIGIKAH 
      HVKLPQTTT EGELLSKLE KLNDDPNIH GIIVQMPLD 
      TTNKIDPHL ITDAVSPEK DVDGLNTLN EGRTAVGDL 
      LSFIPCTPN GIMELIRHT GIKVEGAEA VILGRSKIV 
      GTPVGELLK WAHATVTIC HSRTRNLEE TCKRADILV 
      VAVGKPELV KGSWVKKGA VVIDCGINA IPDPTKKSG 
      QRLVGDVDY ESAKKVASY ITPVPGGVG PMTVAMLMK 
      NTVQSAQKA LNTLLDAKW NLRIIHMRP QRPVPRDID 
      IARAQQPVN IQSLANDIG IYPSELIPY GNTKAKVSL 
      SVLDRLSDR KNAKYIAVV GMTPTPLGE GKTTTSVGL 
      AQALCAHKG LNAIVTLRQ PSLGPTFGI KGGAAGGGY 
      AQVIPMEDF NLHLTGDIH AITAANNLL AAQIDARIF 
      HEATQADTA LFDRLVPTL KGTRKFSPI QLRRLKKLG 
      ISKTDPNTL TPEEKTRFA RLDIDPKSV TWKRVLDIS 
      DRYLRKVTI GQSPTEKGL TRETGFTIA VASEFMAIL 
      ALASDMKDL KERVSNVVI GFNKRKEPV TVDDLGATG 
      AVTVLLKDA MQPNLMQTL EGTPVLVHA GPFANIAHG 
      CSSVVGDKI AFKLAGESG YVITEGGFG SDIGMEKLF 
      NIKCRTSGD APNCVVLVS TIRALKMHG GGPAVTPGA 
      PLKPEYTQE NVELLEKGL PNLIKHISN GRKFGLPVV 
      VCINLFETD TEAEIKLVQ KAAKENGAF DAVVSSHWA 
      DGGAGSIQL AEAVIKACQ QPSKFKLLY DANKSLDEK 
      IQTIAREMY GAAKVTYTP EVTERLKLY EERGYGKLP 
      ICMAKTSMS FTGDPSIKG APTGFELKV SDVSVSVGA 
      GFVVPIAGE ISTMPGLPT RPALYDIDY NIETGQIEG 
      LF
    CDS sequence
    • >TC010593 RA
      atggcatcg actgacgta cttggacaa gtcctatca 
      gggacactt gtggctaaa caacttacg gataaactc 
      acccaggat gtagcccgg cttaagcaa agactgccg 
      ggttttgag ccaggttta gctattgtt caagtcggg 
      ggtcgagag gattcaaac gtttatatt agaatgaaa 
      attaaaact gctggtgag atcggcatc aaagcacat 
      catgttaaa ttaccccaa accacgacg gaaggagag 
      cttctttca aaattggaa aaactgaac gatgacccc 
      aacattcac ggcattatc gtgcaaatg ccccttgat 
      acaactaac aaaattgac ccccattta atcacagac 
      gcggtatcg cccgaaaaa gatgtagac ggtttaaac 
      actctaaat gagggccga acggcggtc ggtgacttg 
      ctgtctttc ataccgtgc acccctaac ggcattatg 
      gaacttatc agacacacc gggatcaaa gttgaaggc 
      gccgaggct gtaattttg ggaaggagc aaaattgtg 
      ggtactcct gttggtgaa cttttaaaa tgggctcac 
      gctactgtg acaatttgc cactcaaga accagaaac 
      ttggaagaa acgtgcaaa agggccgat attttggtg 
      gtggctgtg ggaaaaccg gaactggtg aaagggagc 
      tgggtcaag aagggggct gtggtgatt gactgtggc 
      attaacgcg atcccagat cccactaaa aaatccggc 
      cagagacta gtcggcgat gtcgactac gaatcggcc 
      aagaaggta gcctcctac atcactcca gtccctgga 
      ggtgtaggc cccatgaca gtggcaatg ttaatgaaa 
      aatacggta caaagtgcc cagaaagcc ctcaacacc 
      ttattagat gcaaaatgg aacttgcgc atcattcac 
      atgcgacca caaagaccg gtcccaagg gacatcgat 
      atcgcccgg gcacaacaa cccgtcaac atccaaagt 
      ctagcgaac gatatcggg atatacccc agcgaatta 
      atcccgtac ggcaacacc aaagccaag gtttccttg 
      tcggttctg gaccggcta agcgaccgc aaaaatgcc 
      aaatacatc gcagttgtg gggatgaca ccaactcct 
      ttgggagaa ggcaagacc acaacttcg gtgggcttg 
      gcccaagcc ttgtgcgcc cataagggc ttgaacgcg 
      attgtgacg ctgcgacag ccgtcctta ggcccaact 
      ttcggtatc aaaggaggt gccgctggc ggtggctat 
      gcccaagtg attccaatg gaagatttt aacttgcat 
      ttaaccggc gacatccat gccatcacc gccgctaat 
      aacttacta gcggctcaa attgacgcc cgaattttc 
      cacgaagcc acgcaggcc gacaccgcg ttgttcgac 
      cgcttggtt ccgacccta aaagggacg cgaaaattc 
      tccccaata caactgcgg cgcctcaaa aaacttggc 
      attagtaaa accgaccct aacaccttg acaccagag 
      gagaaaact agattcgct cgcctggat attgaccca 
      aaatcggtg acttggaaa agagtctta gatatcagc 
      gacagatac ctccgtaaa gtcacaatt ggacaatca 
      ccgaccgaa aaaggccta actcgcgaa acgggtttc 
      acaatagcc gtagcaagc gagttcatg gcaatcctt 
      gctttggcg tccgacatg aaggacttg aaggaacgc 
      gtctctaac gtcgttatt ggttttaac aagaggaag 
      gagcccgtg acggttgac gatttgggg gccacgggg 
      gctgttaca gtcctgctc aaagacgca atgcaaccg 
      aatctgatg cagactttg gagggaacc cccgtcctg 
      gtccacgct gggcctttt gccaacata gcccatgga 
      tgttcgtcg gtcgtgggc gataaaatc gccttcaaa 
      ctggccggc gaaagcggc tatgttatc acagagggt 
      ggtttcggg tccgatatc ggaatggaa aagctgttt 
      aatatcaaa tgtcgcacc tcgggagat gccccgaac 
      tgtgtggtt ttggtatca acaattcgg gcgctgaaa 
      atgcacggg ggtggcccc gctgtcact cctggggcc 
      cccctcaag cccgaatac acacaggaa aacgtggaa 
      cttttggaa aagggactt cccaactta atcaaacac 
      atcagcaat ggaaggaaa tttgggctt cccgtcgtt 
      gtttgcatt aatttattc gaaaccgac acggaggcg 
      gagatcaag ctggtccag aaggccgcg aaggagaac 
      ggcgccttc gacgctgtt gtcagcagc cactgggcc 
      gacggaggc gctgggtcc atccagctg gcagaggcc 
      gtcattaaa gcgtgccag caacccagc aaattcaaa 
      cttttgtac gacgcgaac aaatcccta gatgagaaa 
      atccaaacc attgcgagg gagatgtac ggtgcggcc 
      aaagtcacc tacacccct gaagtaaca gaacggttg 
      aagctttac gaagaaagg ggttacggg aaattgcca 
      atttgcatg gcgaaaact tcaatgtcg ttcactgga 
      gatccgagt attaaaggg gcgccgacc ggtttcgag 
      ctgaaagtt tccgatgtt agtgtttcg gtgggggcg 
      ggatttgtg gttccaatt gcaggagag atttccaca 
      atgccgggg ctgcccaca aggccggcg ctgtacgac 
      atcgactac aatattgag accggacaa atcgaggga 
      ttgttttaa
    mRNA sequence
    • >TC010593 RA
      tttaacttc ccttgatta tcttgttgt ttcttggga 
      tagttcatt cggtttcaa ctctttgtc aattccaga 
      ccgtttttt aagaatggc atcgactga cgtacttgg 
      acaagtcct atcagggac acttgtggc taaacaact 
      tacggataa actcaccca ggatgtagc ccggcttaa 
      gcaaagact gccgggttt tgagccagg tttagctat 
      tgttcaagt cgggggtcg agaggattc aaacgttta 
      tattagaat gaaaattaa aactgctgg tgagatcgg 
      catcaaagc acatcatgt taaattacc ccaaaccac 
      gacggaagg agagcttct ttcaaaatt ggaaaaact 
      gaacgatga ccccaacat tcacggcat tatcgtgca 
      aatgcccct tgatacaac taacaaaat tgaccccca 
      tttaatcac agacgcggt atcgcccga aaaagatgt 
      agacggttt aaacactct aaatgaggg ccgaacggc 
      ggtcggtga cttgctgtc tttcatacc gtgcacccc 
      taacggcat tatggaact tatcagaca caccgggat 
      caaagttga aggcgccga ggctgtaat tttgggaag 
      gagcaaaat tgtgggtac tcctgttgg tgaactttt 
      aaaatgggc tcacgctac tgtgacaat ttgccactc 
      aagaaccag aaacttgga agaaacgtg caaaagggc 
      cgatatttt ggtggtggc tgtgggaaa accggaact 
      ggtgaaagg gagctgggt caagaaggg ggctgtggt 
      gattgactg tggcattaa cgcgatccc agatcccac 
      taaaaaatc cggccagag actagtcgg cgatgtcga 
      ctacgaatc ggccaagaa ggtagcctc ctacatcac 
      tccagtccc tggaggtgt aggccccat gacagtggc 
      aatgttaat gaaaaatac ggtacaaag tgcccagaa 
      agccctcaa caccttatt agatgcaaa atggaactt 
      gcgcatcat tcacatgcg accacaaag accggtccc 
      aagggacat cgatatcgc ccgggcaca acaacccgt 
      caacatcca aagtctagc gaacgatat cgggatata 
      ccccagcga attaatccc gtacggcaa caccaaagc 
      caaggtttc cttgtcggt tctggaccg gctaagcga 
      ccgcaaaaa tgccaaata catcgcagt tgtggggat 
      gacaccaac tcctttggg agaaggcaa gaccacaac 
      ttcggtggg cttggccca agccttgtg cgcccataa 
      gggcttgaa cgcgattgt gacgctgcg acagccgtc 
      cttaggccc aactttcgg tatcaaagg aggtgccgc 
      tggcggtgg ctatgccca agtgattcc aatggaaga 
      ttttaactt gcatttaac cggcgacat ccatgccat 
      caccgccgc taataactt actagcggc tcaaattga 
      cgcccgaat tttccacga agccacgca ggccgacac 
      cgcgttgtt cgaccgctt ggttccgac cctaaaagg 
      gacgcgaaa attctcccc aatacaact gcggcgcct 
      caaaaaact tggcattag taaaaccga ccctaacac 
      cttgacacc agaggagaa aactagatt cgctcgcct 
      ggatattga cccaaaatc ggtgacttg gaaaagagt 
      cttagatat cagcgacag atacctccg taaagtcac 
      aattggaca atcaccgac cgaaaaagg cctaactcg 
      cgaaacggg tttcacaat agccgtagc aagcgagtt 
      catggcaat ccttgcttt ggcgtccga catgaagga 
      cttgaagga acgcgtctc taacgtcgt tattggttt 
      taacaagag gaaggagcc cgtgacggt tgacgattt 
      gggggccac gggggctgt tacagtcct gctcaaaga 
      cgcaatgca accgaatct gatgcagac tttggaggg 
      aacccccgt cctggtcca cgctgggcc ttttgccaa 
      catagccca tggatgttc gtcggtcgt gggcgataa 
      aatcgcctt caaactggc cggcgaaag cggctatgt 
      tatcacaga gggtggttt cgggtccga tatcggaat 
      ggaaaagct gtttaatat caaatgtcg cacctcggg 
      agatgcccc gaactgtgt ggttttggt atcaacaat 
      tcgggcgct gaaaatgca cgggggtgg ccccgctgt 
      cactcctgg ggcccccct caagcccga atacacaca 
      ggaaaacgt ggaactttt ggaaaaggg acttcccaa 
      cttaatcaa acacatcag caatggaag gaaatttgg 
      gcttcccgt cgttgtttg cattaattt attcgaaac 
      cgacacgga ggcggagat caagctggt ccagaaggc 
      cgcgaagga gaacggcgc cttcgacgc tgttgtcag 
      cagccactg ggccgacgg aggcgctgg gtccatcca 
      gctggcaga ggccgtcat taaagcgtg ccagcaacc 
      cagcaaatt caaactttt gtacgacgc gaacaaatc 
      cctagatga gaaaatcca aaccattgc gagggagat 
      gtacggtgc ggccaaagt cacctacac ccctgaagt 
      aacagaacg gttgaagct ttacgaaga aaggggtta 
      cgggaaatt gccaatttg catggcgaa aacttcaat 
      gtcgttcac tggagatcc gagtattaa aggggcgcc 
      gaccggttt cgagctgaa agtttccga tgttagtgt 
      ttcggtggg ggcgggatt tgtggttcc aattgcagg 
      agagatttc cacaatgcc ggggctgcc cacaaggcc 
      ggcgctgta cgacatcga ctacaatat tgagaccgg 
      acaaatcga gggattgtt ttaatcgac atttttgta 
      aatgctaaa attttatag gtgacaagg gtgatgcga 
      tattttgta taaataaat aaataaatc cgtgattaa 
      ttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY