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Gene information
General information
TC010591 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 3312625..3314503
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010591
    iBeetle screen iB_01714
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010591 RA
      MDEIKILLS NAIDLMEQN NRGVQLGCY 
      SVALVGLSI AIRRVRPFS RFRRPSDIP 
      NHFIQERKE LTGVVERIE PSGALLMIR 
      HKPLIALPG LPEGQLPVK ISGVNVSGL 
      GLNWLQAIV AGSEVRFIP VAKDRDFVQ 
      CEVLLSQIQ NNKPRVVNV GESLVRIGF 
      GQVVDVDKP ISSDRTFLA YYHRLQGAE 
      KYAKRKKMG LKYYINPTK SALVLAAKS 
      LNSMVLSTY RHIVHIPKM PAS
    CDS sequence
    • >TC010591 RA
      atggacgaa atcaaaatc ttgttaagt aatgccatt 
      gatctaatg gaacagaat aacagaggc gtccagttg 
      ggttgttat tctgtcgca ttagttggc ttgagtatc 
      gccataagg agggtgcgc cccttcagt aggttcagg 
      agaccttcg gacatccct aaccacttc atccaagaa 
      cgcaaagaa ctaaccggg gttgtggaa cgaattgag 
      cccagtggg gcccttctt atgatacgc cacaaaccc 
      ctaatcgcc ttaccagga ctgccagag ggacaactc 
      cctgtcaaa atttcagga gttaatgtt tcaggactt 
      ggcctaaac tggctgcag gctattgtg gcaggaagc 
      gaagtcagg tttattcca gttgcgaaa gaccgagac 
      ttcgttcag tgcgaagtt ttgttgtct cagattcaa 
      aacaacaag ccacgagtt gttaatgtt ggggaatcc 
      cttgttagg attggattt ggccaagtg gtagacgtc 
      gacaagccc atttcgagt gaccgcact tttctggcc 
      tactaccac cgcctccaa ggcgctgag aaatacgca 
      aaacgcaaa aaaatggga ctgaaatat tacataaat 
      ccgacaaag tctgcttta gtcttggca gctaagagc 
      ttgaatagt atggttctt agcacatac agacacatt 
      gttcacata ccgaaaatg ccagcctca tag
    mRNA sequence
    • >TC010591 RA
      tatgaagta taaaaatct agacaccga aagtcaaat 
      atcaaactt caactttta gacttgaat cagaaatct 
      tttggcacc tttggcaag atgtgcatg taattcaac 
      acgttgttg atcgaggat ggacgaaat caaaatctt 
      gttaagtaa tgccattga tctaatgga acagaataa 
      cagaggcgt ccagttggg ttgttattc tgtcgcatt 
      agttggctt gagtatcgc cataaggag ggtgcgccc 
      cttcagtag gttcaggag accttcgga catccctaa 
      ccacttcat ccaagaacg caaagaact aaccggggt 
      tgtggaacg aattgagcc cagtggggc ccttcttat 
      gatacgcca caaacccct aatcgcctt accaggact 
      gccagaggg acaactccc tgtcaaaat ttcaggagt 
      taatgtttc aggacttgg cctaaactg gctgcaggc 
      tattgtggc aggaagcga agtcaggtt tattccagt 
      tgcgaaaga ccgagactt cgttcagtg cgaagtttt 
      gttgtctca gattcaaaa caacaagcc acgagttgt 
      taatgttgg ggaatccct tgttaggat tggatttgg 
      ccaagtggt agacgtcga caagcccat ttcgagtga 
      ccgcacttt tctggccta ctaccaccg cctccaagg 
      cgctgagaa atacgcaaa acgcaaaaa aatgggact 
      gaaatatta cataaatcc gacaaagtc tgctttagt 
      cttggcagc taagagctt gaatagtat ggttcttag 
      cacatacag acacattgt tcacatacc gaaaatgcc 
      agcctcata gcaatcaag gatatactt agcagtcga 
      gatagtact aactatttt accattcat gggcgtgta 
      caaaataat aacttactt tttctatag agacttaca 
      caactgacc gtgagggtg gaatagtag tactttttc 
      gatagtaga cccctttcc tgtatattt atgccatat 
      tgtttactg ttagttgtt aaactcttg caataaaat 
      ttattttgt act