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Gene information
General information
TC010588 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 5368465..5370992
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010588
    iBeetle screen iB_01713

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010588 RA
      MLSQKVFIC ALCKNLLET PVMIVEGIG NMCNTCFTN 
      ADNAEGWTS IPNTNLDLV LKELEFPCT FKSNGCEYT 
      DLYENLHIH KNVCMYRPI NCLFPQSDC NWNGKVIEL 
      LKHFKDQHP QHVLKNPFN KFSFQLEMT KSPINVIKV 
      LLNSHEKIF IFKMWESVE DKVLFNAVQ EIKFCGQGK 
      HVDVKFAGQ NIQCKVSVA RDPFPTVDG AKKICSAIL 
      DEVADSSNT ITVTVKTET GQVQNITEA VLKQLECSV 
      CKELMRPPI VQCESGHSF CSPCKEKVD QCPTCRTKW 
      SNVRNYSLE GITPSLQYP CVYSHVGCE ETFLGNEIV 
      HHELVCKFK LYTCPIADC KFTDNYSLC ANHFRLNHR 
      EFLVEGTVF QDTFTLILN GHETREDKY IFEHENIYK 
      FTFQRLSSS YNWCVRIMN DFSKNRKYY YNVTITDAQ 
      VQQRQISKS VLCLDKKSN DVGITFTSN ELTIYKRTN 
      NDQINYCCE IIEGDEV
    CDS sequence
    • >TC010588 RA
      atgctatcg caaaaagta ttcatctgt gcactttgc 
      aaaaattta ctcgaaacc cctgtaatg atagtcgaa 
      ggtattgga aacatgtgc aatacttgc ttcacaaat 
      gcagataac gcagaaggg tggacttct atccccaac 
      acaaacctg gaccttgtc ttaaaagaa ctcgagttt 
      ccttgcacg tttaaatcg aacggatgt gaatacacg 
      gacctttat gaaaatctg cacatccat aagaacgta 
      tgcatgtat cgcccgatt aactgttta tttccacaa 
      tcagattgc aactggaat ggaaaagta atagagtta 
      ctaaaacac tttaaagac caacacccg caacatgtt 
      ttgaaaaat ccattcaac aaattttcc ttccaactc 
      gagatgaca aagtctcca ataaacgtg ataaaagtg 
      ttgttaaac agtcacgag aaaatattc atcttcaaa 
      atgtgggaa tctgtagaa gataaagtt ttgttcaat 
      gcagttcaa gagattaaa ttttgcggc caaggaaaa 
      cacgtggac gtgaagttt gctggacaa aacatccaa 
      tgcaaagta tcagtggct cgagatcct tttcccacc 
      gtagacggt gccaaaaaa atatgttct gcaatttta 
      gatgaagtc gcagattca agtaacact attacagtg 
      acagtgaag acagagact ggtcaagtc caaaatata 
      accgaggcg gtattaaaa caactcgag tgctccgtg 
      tgcaaggag ctaatgcgc ccccctatt gtccagtgc 
      gagtcaggc cacagtttt tgcagcccc tgtaaagag 
      aaggtggac cagtgccca acatgtcgc acaaaatgg 
      agtaatgtg aggaactac tctcttgaa gggatcacc 
      ccatctctc caatatccg tgcgtctac agccacgta 
      ggatgcgag gagactttc cttggtaat gaaattgtc 
      catcacgaa cttgtgtgc aaattcaaa ctgtacacc 
      tgcccaatc gccgattgc aaatttacc gacaattac 
      tcactatgc gcaaaccac ttcagactc aaccacagg 
      gaattcttg gttgaagga actgttttt caagacact 
      tttacccta atcttaaat ggacacgaa acccgggaa 
      gacaaatac atctttgaa catgaaaat atctacaag 
      tttactttt caacgttta tccagtagc tacaactgg 
      tgcgttcgc attatgaat gattttagc aaaaatcgt 
      aagtattac tacaacgtt acgatcaca gatgcccaa 
      gtgcagcaa agacaaatt tctaaatca gttctttgc 
      ttggataaa aaaagcaat gatgttggc atcactttt 
      acatccaat gaacttact atctataaa cgaacaaac 
      aatgatcaa attaactat tgttgcgag atcatagaa 
      ggtgacgaa gtttaa
    mRNA sequence
    • >TC010588 RA
      gagaaaata ttgtgaatt tgcttcgaa gtcaaaaca 
      ttctttatt cttttttca acacttgtt aagttcttg 
      ttctttgtg taattgagc agagttaat acgaggaag 
      aataaacca acccaacaa taggccaat agtgcgaaa 
      atgcagaat aataaattt accagtaag atgctatcg 
      caaaaagta ttcatctgt gcactttgc aaaaattta 
      ctcgaaacc cctgtaatg atagtcgaa ggtattgga 
      aacatgtgc aatacttgc ttcacaaat gcagataac 
      gcagaaggg tggacttct atccccaac acaaacctg 
      gaccttgtc ttaaaagaa ctcgagttt ccttgcacg 
      tttaaatcg aacggatgt gaatacacg gacctttat 
      gaaaatctg cacatccat aagaacgta tgcatgtat 
      cgcccgatt aactgttta tttccacaa tcagattgc 
      aactggaat ggaaaagta atagagtta ctaaaacac 
      tttaaagac caacacccg caacatgtt ttgaaaaat 
      ccattcaac aaattttcc ttccaactc gagatgaca 
      aagtctcca ataaacgtg ataaaagtg ttgttaaac 
      agtcacgag aaaatattc atcttcaaa atgtgggaa 
      tctgtagaa gataaagtt ttgttcaat gcagttcaa 
      gagattaaa ttttgcggc caaggaaaa cacgtggac 
      gtgaagttt gctggacaa aacatccaa tgcaaagta 
      tcagtggct cgagatcct tttcccacc gtagacggt 
      gccaaaaaa atatgttct gcaatttta gatgaagtc 
      gcagattca agtaacact attacagtg acagtgaag 
      acagagact ggtcaagtc caaaatata accgaggcg 
      gtattaaaa caactcgag tgctccgtg tgcaaggag 
      ctaatgcgc ccccctatt gtccagtgc gagtcaggc 
      cacagtttt tgcagcccc tgtaaagag aaggtggac 
      cagtgccca acatgtcgc acaaaatgg agtaatgtg 
      aggaactac tctcttgaa gggatcacc ccatctctc 
      caatatccg tgcgtctac agccacgta ggatgcgag 
      gagactttc cttggtaat gaaattgtc catcacgaa 
      cttgtgtgc aaattcaaa ctgtacacc tgcccaatc 
      gccgattgc aaatttacc gacaattac tcactatgc 
      gcaaaccac ttcagactc aaccacagg gaattcttg 
      gttgaagga actgttttt caagacact tttacccta 
      atcttaaat ggacacgaa acccgggaa gacaaatac 
      atctttgaa catgaaaat atctacaag tttactttt 
      caacgttta tccagtagc tacaactgg tgcgttcgc 
      attatgaat gattttagc aaaaatcgt aagtattac 
      tacaacgtt acgatcaca gatgcccaa gtgcagcaa 
      agacaaatt tctaaatca gttctttgc ttggataaa 
      aaaagcaat gatgttggc atcactttt acatccaat 
      gaacttact atctataaa cgaacaaac aatgatcaa 
      attaactat tgttgcgag atcatagaa ggtgacgaa 
      gtttaatta aatctttcc gtattcaga gaatttaag 
      ttttacaac tttgtgtcc ttttttgtc gcaaatttt 
      gtttctttt gttactaat ctatcattt ttatagtaa 
      cgttcattt tagtgtcac gtttatttt ttttccaat 
      ttaatactt tgttatttt ataacattt attttattt 
      tatattgtt aatatttta taaaaaccg agaagtgtt 
      tttataata cctacttat gtttatttg tttttgtct 
      tatttttac gttatttac atgtagcca ataaacatc 
      tttattaaa c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY