Status
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Gene information
General information
TC030964
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010566,TC010567
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451448.1: 27775..56968
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030964
    iBeetle screen iB_01711

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030964 RA
      MNFATILIY QFLTCICFV ISGVVNRQC LKKCCSNDS 
      ILSSEIGQC AKANETVPT NYKNVCLVS STNCKNKTV 
      IPIPEEHIQ ILDNNTLVY DFEQRKVLL DPESYCIDF 
      LDSVVNHTV LGCFDSPQS QNIKELGET TTCSLSDFC 
      KSKVPFVVN IDNFTIIEN SLKYSFFGQ LTQFDEFCI 
      NPSARTALV CISVPKNDL AKLSPGDDC IRKCCPHDS 
      LISNVTKNC VQLNQTVQL PYENVCRVT KKTCFGDKF 
      PAEIPQESF TIAENYDLV YTYNEVSVI FAKDDYCLD 
      FTNQMEFLS ALVCVESNN PGNSAPIVT NEFQLKMGA 
      SNLKFVIFV LFKVTLICA SETDCETNK CIRKCCADD 
      EFLNFDKSS LKCDLNFGK SLRDLANIS LYQIIHGKT 
      CDPELMKIP LDGVDMPFT LDGNKVLWE NMEFNHTTS 
      CIDVYGDKV VALVCTLGD EEEQLMAAI GQQFVNKKC 
      KNVGDDADA NLVKTAKLP HSAHVTIRS NYCDVSSKI 
      LFVINEDEE ECSVRWNNK LYYRQEFLD YFCVDYNAD 
      NGGLRVLVC EDKCVYHAA LLFFLAQIQ KTSTFDFCD 
      TKSAILSNT TSYSYKGQQ EENCNCVRR TSCLRKCCA 
      VGFYASKGV CLRYDNPDF VLNIPVYDG KKFVRNANA 
      DTNVLAGVM SCDFFIFES NEKDWFYPQ DKGGILTSE 
      YGLVENDFY CVDDFKDKG LAAFICYDR VTTEATSRK 
      IYAAGMIIS MPFLLLTFI AYALLPDRN LHMKALMFY 
      VINLMFSYL LLVIIQLSE SQFAEATCK SLGYFCLFF 
      FLVSFFWMN VICIDIWWG FSGLRGFTG SKKTAERKR 
      FLFYCAYAW GMPVLHVLV VFIIDILAN QNSVLKPDI 
      GNGQCFLRR GFPELLYFY GPMAILITI NIVLFAITA 
      IKIRRIKSE TAMLKKEDS KRHSYENDR QIFNLYVKL 
      LFAMGVNWS MEIISWAVI WAEEAPEAI WYLTDFCNA 
      IYGVFIFFI FVFKRSIWK LLKKRYYTF IGKHHLAHS 
      MTMTNTTRV TSYSTATST IQETAHGGD NDTSTLKHD 
      P
    CDS sequence
    • >TC030964 RA
      atgaatttc gctacgatc ctaatctat cagtttctc 
      acttgtatt tgttttgtg atcagtgga gttgtaaac 
      agacaatgt ttaaagaag tgttgttcg aatgattcg 
      attttgtcg tcggaaatc ggccaatgt gccaaagcc 
      aatgaaacc gttccaact aactacaaa aatgtttgt 
      ttggtgtct tccacaaac tgcaaaaac aaaaccgtc 
      atcccaatc ccggaagaa cacatccag atacttgac 
      aacaacact ctcgtgtac gattttgag caacgcaaa 
      gtgcttttg gaccctgag tcttactgc atcgatttt 
      ttggacagt gtggtaaat cacacggtt ctggggtgc 
      tttgacagc ccccagagc caaaatatc aaagaatta 
      ggtgagaca acgacctgt tccctttct gatttttgc 
      aagagcaaa gtgcccttt gttgtgaac atcgacaac 
      tttacgatt attgaaaac agtctaaaa tattcgttc 
      ttcggccag ttgacgcaa tttgacgag ttttgtatc 
      aacccaagt gcaagaacg gcgctggtg tgcatctca 
      gtgccaaaa aatgatctc gctaagctc tcacctgga 
      gacgattgc ataaggaag tgctgtccg cacgattcg 
      cttatttcc aatgtcacc aaaaattgt gttcaactg 
      aaccaaact gtccaattg ccctacgag aacgtttgt 
      cgtgtgacc aaaaaaacg tgttttggc gacaaattc 
      cctgcggaa atacctcaa gagtcgttc actattgcc 
      gagaattat gatttggtt tatacgtac aacgaggtt 
      tcggtgatt tttgcgaaa gacgactac tgtttagac 
      tttacgaac caaatggaa tttctctcg gcgttagtt 
      tgtgttgaa agtaacaat ccgggaaat tcggctccg 
      atagtaacg aacgaattt caactcaaa atgggtgct 
      agtaactta aagtttgtt attttcgta ctgtttaaa 
      gttaccttg atttgtgcc agtgaaaca gactgtgaa 
      accaacaag tgtatcaga aaatgttgc gccgacgac 
      gaatttctg aactttgat aaaagttcc ctaaaatgt 
      gacctaaac tttgggaaa tccctacgc gatttggcg 
      aatatttcc ctgtatcaa ataattcac gggaaaact 
      tgtgaccca gaactgatg aaaataccc cttgacggt 
      gtagatatg ccctttact ttggacggg aataaagtg 
      ttgtgggaa aatatggaa tttaatcac acaacttct 
      tgcattgat gtttatggg gataaagtg gtggcgctt 
      gtttgcacg ttaggggat gaagaagaa caactcatg 
      gcagctata ggccagcaa tttgttaat aaaaagtgc 
      aaaaacgtg ggtgatgat gccgatgct aatttagtt 
      aaaacagcc aagcttccg cattcggca catgtgaca 
      attcggagc aattattgt gatgttagt agcaaaatt 
      ctgttcgtg ataaatgaa gatgaagag gaatgcagt 
      gttcggtgg aacaacaaa ttgtactac agacaggag 
      tttttggat tatttttgt gtggattat aacgctgat 
      aatggaggt ttacgagtg ttggtgtgc gaggataaa 
      tgcgtttat cacgcggct ttgctcttc ttccttgca 
      caaatccaa aaaacctcg acttttgat ttctgtgat 
      actaaaagt gcaatctta agcaacacc acatcctat 
      tcctacaaa ggccaacaa gaggaaaac tgcaactgt 
      gtgcggaga acttcttgt ttgcgaaaa tgttgtgcg 
      gtgggattt tacgccagc aaaggagtg tgtcttcgt 
      tatgataat ccggatttt gttttgaac attccggtt 
      tacgatggg aaaaaattc gtgcgcaat gcgaacgct 
      gatacgaac gttttggcg ggagttatg agctgtgat 
      tttttcatt ttcgaatcg aacgaaaag gattggttt 
      tatccgcag gacaagggg gggatttta acgtccgag 
      tacggttta gtggaaaat gatttttac tgtgttgat 
      gattttaag gataagggg ttggctgcg tttatttgc 
      tatgatagg gtcaccacg gaagccacc tctcgaaaa 
      atatacgct gcaggtatg ataatatct atgccattc 
      ctgctacta acattcatc gcatatgct cttctaccg 
      gatcggaat ctacatatg aaagctctt atgttttat 
      gtcatcaac ctgatgttc agttacctg cttctggtc 
      atcatccag ctttcagaa agtcaattt gctgaagct 
      acttgcaaa tctttaggg tacttctgc ctgtttttc 
      tttttggtg tcgttcttt tggatgaac gtcatttgc 
      attgatata tggtggggt ttcagtgga ttaagaggt 
      ttcactggt agcaagaaa acagcggaa agaaaacga 
      tttcttttt tattgtgcc tatgcctgg ggaatgcca 
      gtacttcat gttttagtt gttttcatt atcgacatt 
      ttggcaaac cagaattca gttcttaaa ccagacatc 
      ggtaatgga caatgcttt ttgagacgt ggttttccc 
      gaactgttg tatttctat ggaccaatg gctatatta 
      ataacgata aatatagtg ctgtttgcc attactgcc 
      attaaaata cggagaata aagagcgaa actgcaatg 
      ctgaagaaa gaagacagt aaacgtcac tcttatgaa 
      aatgataga caaattttc aatctctac gtcaaactg 
      ctttttgca atgggtgtg aactggtca atggaaata 
      atatcatgg gctgtgatt tgggcagaa gaggctcct 
      gaagcgatc tggtatctg actgatttc tgcaatgcc 
      atttacgga gtgttcatt ttcttcatt ttcgtattc 
      aagcggagc atttggaag ttgttgaag aaacgttat 
      tatacattt attggtaaa catcacttg gcccactcc 
      atgacgatg actaacacg acaagagtt acaagttat 
      tcgaccgcc acttcaacc attcaggaa actgcccat 
      gggggtgat aacgacacc agcactttg aaacatgac 
      ccatga
    mRNA sequence
    • >TC030964 RA
      gcgtcatta aacgcacaa tgaatttcg ctacgatcc 
      taatctatc agtttctca cttgtattt gttttgtga 
      tcagtggag ttgtaaaca gacaatgtt taaagaagt 
      gttgttcga atgattcga ttttgtcgt cggaaatcg 
      gccaatgtg ccaaagcca atgaaaccg ttccaacta 
      actacaaaa atgtttgtt tggtgtctt ccacaaact 
      gcaaaaaca aaaccgtca tcccaatcc cggaagaac 
      acatccaga tacttgaca acaacactc tcgtgtacg 
      attttgagc aacgcaaag tgcttttgg accctgagt 
      cttactgca tcgattttt tggacagtg tggtaaatc 
      acacggttc tggggtgct ttgacagcc cccagagcc 
      aaaatatca aagaattag gtgagacaa cgacctgtt 
      ccctttctg atttttgca agagcaaag tgccctttg 
      ttgtgaaca tcgacaact ttacgatta ttgaaaaca 
      gtctaaaat attcgttct tcggccagt tgacgcaat 
      ttgacgagt tttgtatca acccaagtg caagaacgg 
      cgctggtgt gcatctcag tgccaaaaa atgatctcg 
      ctaagctct cacctggag acgattgca taaggaagt 
      gctgtccgc acgattcgc ttatttcca atgtcacca 
      aaaattgtg ttcaactga accaaactg tccaattgc 
      cctacgaga acgtttgtc gtgtgacca aaaaaacgt 
      gttttggcg acaaattcc ctgcggaaa tacctcaag 
      agtcgttca ctattgccg agaattatg atttggttt 
      atacgtaca acgaggttt cggtgattt ttgcgaaag 
      acgactact gtttagact ttacgaacc aaatggaat 
      ttctctcgg cgttagttt gtgttgaaa gtaacaatc 
      cgggaaatt cggctccga tagtaacga acgaatttc 
      aactcaaaa tgggtgcta gtaacttaa agtttgtta 
      ttttcgtac tgtttaaag ttaccttga tttgtgcca 
      gtgaaacag actgtgaaa ccaacaagt gtatcagaa 
      aatgttgcg ccgacgacg aatttctga actttgata 
      aaagttccc taaaatgtg acctaaact ttgggaaat 
      ccctacgcg atttggcga atatttccc tgtatcaaa 
      taattcacg ggaaaactt gtgacccag aactgatga 
      aaatacccc ttgacggtg tagatatgc cctttactt 
      tggacggga ataaagtgt tgtgggaaa atatggaat 
      ttaatcaca caacttctt gcattgatg tttatgggg 
      ataaagtgg tggcgcttg tttgcacgt taggggatg 
      aagaagaac aactcatgg cagctatag gccagcaat 
      ttgttaata aaaagtgca aaaacgtgg gtgatgatg 
      ccgatgcta atttagtta aaacagcca agcttccgc 
      attcggcac atgtgacaa ttcggagca attattgtg 
      atgttagta gcaaaattc tgttcgtga taaatgaag 
      atgaagagg aatgcagtg ttcggtgga acaacaaat 
      tgtactaca gacaggagt ttttggatt atttttgtg 
      tggattata acgctgata atggaggtt tacgagtgt 
      tggtgtgcg aggataaat gcgtttatc acgcggctt 
      tgctcttct tccttgcac aaatccaaa aaacctcga 
      cttttgatt tctgtgata ctaaaagtg caatcttaa 
      gcaacacca catcctatt cctacaaag gccaacaag 
      aggaaaact gcaactgtg tgcggagaa cttcttgtt 
      tgcgaaaat gttgtgcgg tgggatttt acgccagca 
      aaggagtgt gtcttcgtt atgataatc cggattttg 
      ttttgaaca ttccggttt acgatggga aaaaattcg 
      tgcgcaatg cgaacgctg atacgaacg ttttggcgg 
      gagttatga gctgtgatt ttttcattt tcgaatcga 
      acgaaaagg attggtttt atccgcagg acaaggggg 
      ggattttaa cgtccgagt acggtttag tggaaaatg 
      atttttact gtgttgatg attttaagg ataaggggt 
      tggctgcgt ttatttgct atgataggg tcaccacgg 
      aagccacct ctcgaaaaa tatacgctg caggtatga 
      taatatcta tgccattcc tgctactaa cattcatcg 
      catatgctc ttctaccgg atcggaatc tacatatga 
      aagctctta tgttttatg tcatcaacc tgatgttca 
      gttacctgc ttctggtca tcatccagc tttcagaaa 
      gtcaatttg ctgaagcta cttgcaaat ctttagggt 
      acttctgcc tgtttttct ttttggtgt cgttctttt 
      ggatgaacg tcatttgca ttgatatat ggtggggtt 
      tcagtggat taagaggtt tcactggta gcaagaaaa 
      cagcggaaa gaaaacgat ttctttttt attgtgcct 
      atgcctggg gaatgccag tacttcatg ttttagttg 
      ttttcatta tcgacattt tggcaaacc agaattcag 
      ttcttaaac cagacatcg gtaatggac aatgctttt 
      tgagacgtg gttttcccg aactgttgt atttctatg 
      gaccaatgg ctatattaa taacgataa atatagtgc 
      tgtttgcca ttactgcca ttaaaatac ggagaataa 
      agagcgaaa ctgcaatgc tgaagaaag aagacagta 
      aacgtcact cttatgaaa atgatagac aaattttca 
      atctctacg tcaaactgc tttttgcaa tgggtgtga 
      actggtcaa tggaaataa tatcatggg ctgtgattt 
      gggcagaag aggctcctg aagcgatct ggtatctga 
      ctgatttct gcaatgcca tttacggag tgttcattt 
      tcttcattt tcgtattca agcggagca tttggaagt 
      tgttgaaga aacgttatt atacattta ttggtaaac 
      atcacttgg cccactcca tgacgatga ctaacacga 
      caagagtta caagttatt cgaccgcca cttcaacca 
      ttcaggaaa ctgcccatg ggggtgata acgacacca 
      gcactttga aacatgacc catgaggcg tcttaaagg 
      tgcttaata cggtagcag gagagtcca acaaatgac 
      caaagattt atctattct taaattgaa actaataaa 
      tgtaaagtt cgtattaca ttggagaat aattttaac 
      acacgtatc gcgcacgat gatttccat gacattagt 
      ttagagtat ttttatatt tatattttt aatacgtct 
      ccaaaaatt gtacccaag tattttatc atgtattcg 
      atttttgtg gaaacagca ctccttttt aaatgttta 
      aatttttgc aaaataaac tgtattacc tacttttta 
      ttggttttg atttttggc attgtgctt tatttctgg 
      tgcgaatag acttaagtt agatttgcg catgtaacg 
      tcagctgct gagcggaaa aaggaaacc tttttcatt 
      tttttttat ttaatttaa aaagataac aattttttg 
      tctgtgatt ttgttctga taaaaaata aacatcact 
      gaacattgg atttagata aatgagaat attcctttt 
      agtttccct tttccattc agcactagc ctcaactat 
      tcattgtat tccttctag aaccataac ctgttctcg 
      ttatcttaa ttatttaga taaatcaaa aatgtattt 
      tgctttgta actaatcgt cttactttt gtttcacaa 
      acgtttatc attacctaa gtatttaaa cccgatata 
      attatgtgt atgtatgtg taattgtga atgataaat 
      caaatacag tacataaac tagtcgagt gtttaggat 
      tttattttt gtaaaagaa ttattttta ttctttaat 
      taatgtata cgcatatca ccgaattta aaaccctag 
      tcagatttt tgatttcct tttttaagt taagaatat 
      ttttcagta cttaatttt tcaggtttt tttatataa 
      ttcttcttt tattgcaaa aaaagtatt ccgttttat 
      gtgttttgt acaactata taaatatgt agcttttgc 
      aataataaa gttattgat tattg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY