Status
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Gene information
General information
TC010557 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 15600898..15607106
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010557
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01709

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010557 RA
      MGIKFLEVI KPFCSILPE IAKPERKIQ FREKVLWTA 
      ITLFIFLVC CQIPLFGIM SSDSADPFY WIRVILASN 
      RGTLMELGI SPIVTSGLI MQLLAGAKI IEVGDTPKD 
      RALFNGAQK LFGMVITVG QAIVYVMTG MYGDPAEIG 
      AGVCLLIII QLFVAGLIV LLLDELLQK GYGLGSGIS 
      LFIATNICE TIIWKAFSP ATVNTGRGT EFEGAVIAL 
      FHLLTTRQD KIRALREAF YRQNLPNLM NLLATVLVF 
      AIVIYFQGF RVDLPIKSA RYRGQYSSY PIKLFYTSN 
      IPIILQSAL VSNLYVISQ MLAVKFQGN FLINLLGVW 
      ADVGGGGPA RSYPIGGLC YYLSPPESV GHILEDPIH 
      ALLYIVFML GSCAFFSKT WIEVSGSSA KDVAKQLKE 
      QQMVMRGHR DNSMIHELN RYIPTAAAF GGLCIGALS 
      VLADFLGAI GSGTGILLA VTIIYQYFE IFVKEQSDM 
      GGMGALLF
    CDS sequence
    • >TC010557 RA
      atggggatc aaattttta gaagtaatc aaaccgttc 
      tgcagtatc ctgcctgaa atagccaag cctgaaaga 
      aagattcaa ttcagggag aaagtacta tggacggcg 
      atcacactt ttcattttt ctcgtatgt tgtcagatt 
      ccactgttt ggcattatg agttcagac tcggccgat 
      ccgttttat tggatacgt gtaatttta gcttcaaac 
      agaggaacc ctgatggag ttgggtatt tccccgatt 
      gttacttcc ggattgatt atgcaactt ctagcagga 
      gcgaagata attgaagtt ggggataca ccgaaagac 
      cgagctttg ttcaacggc gcacaaaaa ttattcggt 
      atggtcatc accgtgggc caagccatc gtatacgta 
      atgaccggc atgtacgga gaccccgcc gaaataggc 
      gcaggtgta tgtctctta atcatcatc caactgttc 
      gttgctggt ctcattgtt ctactcctc gacgaactc 
      ctccaaaaa ggctacggt cttggttct ggcatttcc 
      ctcttcatt gcaaccaac atttgtgaa acaatcatc 
      tggaaggcg ttctcacca gccacagta aacaccggc 
      cgaggaacg gaattcgaa ggcgctgta atcgccctc 
      ttccaccta ctgaccaca cgtcaggac aaaatccga 
      gcacttcgt gaggccttc taccgtcaa aatctaccc 
      aacttgatg aatcttcta gccactgtt ctcgttttt 
      gcgatagtt atatacttc caagggttc agggttgat 
      ctcccaatc aaaagtgcg agataccgt ggccagtac 
      tcgagttat ccgatcaaa cttttctac acttctaat 
      atccccatt attttgcaa tcagctctg gtttcgaat 
      ctttacgtt atttcgcaa atgttggcc gtcaaattc 
      caaggcaac ttccttatt aatttatta ggggtttgg 
      gctgatgtt ggtggtggg ggcccagct cgctcttat 
      ccaatcgga ggtctctgt tactacctc tcccctcct 
      gaaagcgtc ggtcatatt cttgaagac ccgattcac 
      gcacttttg tacattgtc ttcatgttg ggttcttgt 
      gccttcttc tccaagacg tggatcgag gtgtccgga 
      agttcagca aaagatgta gcgaagcag ttgaaggag 
      caacagatg gtcatgagg ggacatcgc gataattcg 
      atgattcac gaactgaac cggtatatt ccgacagca 
      gcagctttc ggcgggctt tgtatcgga gctctgtct 
      gttttggcc gatttcttg ggtgcgatt ggttcaggc 
      accggtatt ttgctggct gtcactatt atttaccag 
      tacttcgaa atttttgtt aaagaacag agcgatatg 
      ggcggcatg ggggccctt ctattttaa
    mRNA sequence
    • >TC010557 RA
      tgtaaccaa aatcgctct cacaaagta ccgtaaatc 
      gggacttgg ccacttatt tgacgtgac atcgtagaa 
      attagactt atccgaagt aaatctgtg atcctttta 
      gtttaattc agtgcccat caaaatggg gatcaaatt 
      tttagaagt aatcaaacc gttctgcag tatcctgcc 
      tgaaatagc caagcctga aagaaagat tcaattcag 
      ggagaaagt actatggac ggcgatcac acttttcat 
      ttttctcgt atgttgtca gattccact gtttggcat 
      tatgagttc agactcggc cgatccgtt ttattggat 
      acgtgtaat tttagcttc aaacagagg aaccctgat 
      ggagttggg tatttcccc gattgttac ttccggatt 
      gattatgca acttctagc aggagcgaa gataattga 
      agttgggga tacaccgaa agaccgagc tttgttcaa 
      cggcgcaca aaaattatt cggtatggt catcaccgt 
      gggccaagc catcgtata cgtaatgac cggcatgta 
      cggagaccc cgccgaaat aggcgcagg tgtatgtct 
      cttaatcat catccaact gttcgttgc tggtctcat 
      tgttctact cctcgacga actcctcca aaaaggcta 
      cggtcttgg ttctggcat ttccctctt cattgcaac 
      caacatttg tgaaacaat catctggaa ggcgttctc 
      accagccac agtaaacac cggccgagg aacggaatt 
      cgaaggcgc tgtaatcgc cctcttcca cctactgac 
      cacacgtca ggacaaaat ccgagcact tcgtgaggc 
      cttctaccg tcaaaatct acccaactt gatgaatct 
      tctagccac tgttctcgt ttttgcgat agttatata 
      cttccaagg gttcagggt tgatctccc aatcaaaag 
      tgcgagata ccgtggcca gtactcgag ttatccgat 
      caaactttt ctacacttc taatatccc cattatttt 
      gcaatcagc tctggtttc gaatcttta cgttatttc 
      gcaaatgtt ggccgtcaa attccaagg caacttcct 
      tattaattt attaggggt ttgggctga tgttggtgg 
      tgggggccc agctcgctc ttatccaat cggaggtct 
      ctgttacta cctctcccc tcctgaaag cgtcggtca 
      tattcttga agacccgat tcacgcact tttgtacat 
      tgtcttcat gttgggttc ttgtgcctt cttctccaa 
      gacgtggat cgaggtgtc cggaagttc agcaaaaga 
      tgtagcgaa gcagttgaa ggagcaaca gatggtcat 
      gaggggaca tcgcgataa ttcgatgat tcacgaact 
      gaaccggta tattccgac agcagcagc tttcggcgg 
      gctttgtat cggagctct gtctgtttt ggccgattt 
      cttgggtgc gattggttc aggcaccgg tattttgct 
      ggctgtcac tattattta ccagtactt cgaaatttt 
      tgttaaaga acagagcga tatgggcgg catgggggc 
      ccttctatt ttaaagcaa tcgaaatat cgtgcgtgc 
      gatattgtg attttctta ttgttaatt gtaaatgtt 
      gactgtcgc atttgctca ttcttacag gtgtagatt 
      tagttaatt gaggatgcg acagttaac gttttgtct 
      tgatcaggt gtgatcaat tttttgtac taagaagat 
      aatattaca aatattttt tcattgtat attcacatt 
      tagttcccc tctgcattt tatggatgt tgatatttg 
      tgaatggcg ctccgtaag acacgtctg gtatttaca 
      tacttgcat cattttcat gcaaacggt tgtgtgttt 
      tggtcattt tgttataga ttataattt attttaaat 
      acaactaat ttccataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY