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Gene information
General information
TC010548 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 15382674..15387524
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010548
    iBeetle screen iB_01708

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010548 RA
      MSSVRSASS HTYYSNTRS HLSNDQEHQ SLPPLQVIL 
      EEKNRVIDK LKKQVHNQR IQLEFYIKK EMNSPLMVE 
      FAKTCLPEL TDDNMTVAL AMNKFYREL GMKSRIMEL 
      YDRVCNIAG RTNCVIQTQ EVILNEIIS NMKQYMSPA 
      EIKKFLGNF DINTNYIEI FNSLLDTTI SLKSSMNGF 
      RKCCDRLIN AEEKRNEIV EQVYSLPQD ERTICNYLV 
      NVQMEKFEL ENLVKQLSK KRKILDPTV DEIDIIENN 
      LKLLHDNFD SLQEQDETD YCVTKMLEK VYSQAMSLL 
      SLSREQREK YNKEVDQLK EENEKLREI IQHIDNRSS 
      VGKCVTTDL NDIEKLHNV IKKTISEAR KFEKTKTEE 
      LKQKSQECE DLKKKLSEV VMQKRQLQT LLDSESTEE 
      NEFILEHES PSLSYKTQV ENLQTICGA HSA
    CDS sequence
    • >TC010548 RA
      atgagcagc gtccgaagt gccagtagc cacacttac 
      tactccaac acgcgatct catctcagc aatgatcag 
      gaacatcag tctcttccg cccctgcaa gtcatcctc 
      gaggaaaaa aatcgtgtt attgacaag ctcaaaaaa 
      caggtccac aatcaaaga atccagctc gagttttac 
      atcaagaag gaaatgaac agtccgctt atggtggaa 
      tttgccaaa acgtgctta cctgaactc acggacgat 
      aatatgacc gtggcttta gctatgaac aaattctac 
      agagaatta ggaatgaaa agccggata atggagctt 
      tatgaccgt gtttgcaac attgctggc cgaacgaat 
      tgtgttatt cagactcag gaagttatt ctcaatgaa 
      atcatttct aacatgaag cagtacatg agtcctgcc 
      gaaataaaa aagttcttg gggaacttt gacatcaat 
      acaaactat atcgaaata tttaacagt ctactggac 
      acgacaata agtttaaag agttcaatg aacgggttt 
      agaaaatgt tgtgatcgc ttgatcaac gccgaagag 
      aaacgaaat gaaattgtg gagcaagtt tactcgctt 
      cctcaagat gaacgcaca atctgcaac tacttagtc 
      aacgtgcag atggaaaaa ttcgagttg gaaaatttg 
      gtaaaacaa ctgtccaaa aaacgtaaa atactggac 
      cccaccgtt gatgagatc gacataatc gagaataat 
      ctcaagctc cttcatgac aattttgac tctttacag 
      gaacaagac gaaactgat tattgtgtc acaaaaatg 
      ttggagaag gtttactcg caagcaatg tccctcttg 
      tcactctct cgcgagcag cgcgagaaa tacaacaaa 
      gaagtggat caactcaag gaggagaac gaaaagctc 
      cgggaaata atccaacac attgacaac agaagctcg 
      gtcggaaaa tgcgtaaca accgatttg aacgacatc 
      gaaaagcta cacaacgtc atcaagaaa actataagt 
      gaggcccgc aaattcgag aaaacgaaa acggaagag 
      ctgaaacaa aagtcgcaa gagtgtgag gatttgaaa 
      aagaagttg agcgaagtg gtgatgcag aagaggcag 
      ctgcagacg ctgctggat tcggagagt actgaagaa 
      aatgagttc atactggag catgagagt ccaagtctt 
      tcgtataaa acacaagtg gaaaatcta caaacgatt 
      tgtggtgcg cacagtgcg tga
    mRNA sequence
    • >TC010548 RA
      tgctgggga aataattga aacaataaa ttgacgtta 
      cgtcattca ataaaaatg ccagttcaa attcaagta 
      tagatgcac agtttttga atagattag gaaaatgag 
      cagcgtccg aagtgccag tagccacac ttactactc 
      caacacgcg atctcatct cagcaatga tcaggaaca 
      tcagtctct tccgcccct gcaagtcat cctcgagga 
      aaaaaatcg tgttattga caagctcaa aaaacaggt 
      ccacaatca aagaatcca gctcgagtt ttacatcaa 
      gaaggaaat gaacagtcc gcttatggt ggaatttgc 
      caaaacgtg cttacctga actcacgga cgataatat 
      gaccgtggc tttagctat gaacaaatt ctacagaga 
      attaggaat gaaaagccg gataatgga gctttatga 
      ccgtgtttg caacattgc tggccgaac gaattgtgt 
      tattcagac tcaggaagt tattctcaa tgaaatcat 
      ttctaacat gaagcagta catgagtcc tgccgaaat 
      aaaaaagtt cttggggaa ctttgacat caatacaaa 
      ctatatcga aatatttaa cagtctact ggacacgac 
      aataagttt aaagagttc aatgaacgg gtttagaaa 
      atgttgtga tcgcttgat caacgccga agagaaacg 
      aaatgaaat tgtggagca agtttactc gcttcctca 
      agatgaacg cacaatctg caactactt agtcaacgt 
      gcagatgga aaaattcga gttggaaaa tttggtaaa 
      acaactgtc caaaaaacg taaaatact ggaccccac 
      cgttgatga gatcgacat aatcgagaa taatctcaa 
      gctccttca tgacaattt tgactcttt acaggaaca 
      agacgaaac tgattattg tgtcacaaa aatgttgga 
      gaaggttta ctcgcaagc aatgtccct cttgtcact 
      ctctcgcga gcagcgcga gaaatacaa caaagaagt 
      ggatcaact caaggagga gaacgaaaa gctccggga 
      aataatcca acacattga caacagaag ctcggtcgg 
      aaaatgcgt aacaaccga tttgaacga catcgaaaa 
      gctacacaa cgtcatcaa gaaaactat aagtgaggc 
      ccgcaaatt cgagaaaac gaaaacgga agagctgaa 
      acaaaagtc gcaagagtg tgaggattt gaaaaagaa 
      gttgagcga agtggtgat gcagaagag gcagctgca 
      gacgctgct ggattcgga gagtactga agaaaatga 
      gttcatact ggagcatga gagtccaag tctttcgta 
      taaaacaca agtggaaaa tctacaaac gatttgtgg 
      tgcgcacag tgcgtgatg tgaaaattt agtgttgtg 
      aaataaatt ttgttaacc aca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY