Status
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Gene information
General information
TC032522
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010547
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 15221146..15263335
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032522
    iBeetle screen iB_01707

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 leg musculature tb1-ptar1 shortened - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: Muscle defect due to defects in the leg morphology (tibiotarsus looks much sorter than normal)
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs femur shortened - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs tibiotarsus shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    posterior terminus shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    Pupal morphology
    head appendages pattern broader - Number of animals showing the phenotype: 9
    Comment: The distance between the labrum/mandible to the labial and maxillary palps increased!
    labial palp structure irregular - Number of animals showing the phenotype: 9

    Comment: In some cases, the labial palps were completely swollen and maxillary palps seemed to be transformed into antennae!
    antenna orientation bent - Number of animals showing the phenotype: 9

    gena size decreased - Number of animals showing the phenotype: 9
    elytra shortened - Number of animals showing the phenotype: 9

    legs femur structure irregular - Number of animals showing the phenotype: 9

    Comment: Femur and tibia were thicker/fatter than usual.
    legs tibia structure irregular - Number of animals showing the phenotype: 9
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032522 RA
      MATLGLSKV FILDKYFTE LQKFWETEK KLQDASSSN 
      EAVHLQQRL RSLSTELVT LRNRLHVQG APAAPLKGA 
      PVPVVAGTQ PVIPPRSAP TAPLPVVAV PDSRPQLPT 
      AGADLDDLI HLQGPLTED AVMKCLQAR FNASNFYTN 
      VGPILLCVN PYRDVGNPL TLNSTRGLA LSPQLNRVV 
      QEAVRQQSE TGYPQAIIL SGTSGSGKS HASMLLLRQ 
      LFAVAGGGP ETDAFKHLA AAFTVLRSL GSAKTATNS 
      ESSRIGQFI EVQVTDGAL YRTKIHCYF LDQTRVIRP 
      LPGEKNYHI FYQMLAGLS AEERSKLNL EGHTPATLR 
      YLQHGDTRQ DLAEDSSRF QTWKTCLGI LGIPFLDVV 
      RVLAAVLLL GNVQFLDGK GLEVDVKGD TELNAVAGL 
      LGVSGAALF RGLTTRTHN ARGQLVKSV CDANMSNMT 
      RDCLAKALY CRTVATIVR RANSLKRLG STLGTLSSD 
      SNESVHNQA EVTSQHAST VNGSTNAGS KSMAALNNA 
      VRHATDGFI GILDMFGFE DPKPSQLEH LCINLCAET 
      MQHFYNTHI FKSSIESCR DEGINCEVE VDYVDNVPC 
      IDLISSLRT GLLSMLDVE CSIRGTAES YVSKISVQH 
      KHNSRLFEP KPVDPRLFG IQHFAGRVV YDATDFLDT 
      NKDVVPDDL VAVFYKHNC NFGFATHLF GSELKALYS 
      VESVPRGVS FRISPTSHT DLLNGDEPV STLTQDFHT 
      RLDNLLRTL VHARPHFVR CIRSNSQET PNRFDRGTV 
      VRQIRSLQV LETVNLMAG GYPHRMRFK AFNARYRLL 
      APFARLHRT DEKVTDDCH LILQYVVDL ISKQHNTLV 
      STSWALGKR HIFLSEGIR QHLEKLRTE TRQKAATLI 
      QATWRGWHC RYRWAALRR EKEHKSATN TNTLNNRTP 
      IGGAIARPR PQPIAGTPP PDPNEKCDA KIIQQTCNL 
      FGLDLERPP PVPPSRSYT VSGNTKLGY PQTRVMKMS 
      YPEDCNGEG QVLMKGETV LVVGASHRR GHLIVEHKH 
      CTFHVPFQF LELKQSVNI
    CDS sequence
    • >TC032522 RA
      atggctacg ctaggcctc tccaaagtg ttcattttg 
      gacaaatac ttcacggag ctgcagaag ttctgggag 
      acggagaag aagctccaa gatgcgtcg tcgtcgaac 
      gaagccgtc cacctgcag cagcgcctc cgcagcctg 
      agcacggag ctggtgacg ctccgcaac cgcctccat 
      gtgcagggc gcgccggcg gcccccctg aagggcgcc 
      cccgtgccg gtggtggcc ggcacgcag ccggtgatt 
      cccccgcgg agcgcgccg acggcgccg cttcccgtg 
      gtggccgtc cccgacagc cgcccccag ctgcccacc 
      gcgggggcc gacctggac gacctcatc cacctgcag 
      gggcctctc accgaagac gcggtgatg aagtgcctc 
      caggcgcga ttcaacgct tccaacttc tacacaaac 
      gtgggtcca attctcttg tgtgtgaac ccatacaga 
      gacgtcggc aatccatta acactgaac agtactcgc 
      ggcctggcc ttgagcccc caactaaac agggtagtt 
      caggaagct gtccgacag caatccgaa acgggttat 
      ccccaagcc atcatcctt tcaggaacc agcggctcc 
      ggtaagagc catgcttcc atgttgctt ctgcgacaa 
      ctttttgcc gtcgctggc ggaggcccc gaaacggac 
      gctttcaag catttagcc gcggctttc acagtatta 
      cggtcgctg ggctccgct aaaaccgcc accaactcg 
      gagtcgagc cgaattggc cagttcatc gaagtgcag 
      gtcacggac ggggcgctg taccgcacc aaaatccac 
      tgctacttt ctggaccag acgcgcgtc atccggccc 
      ttgcccggc gagaaaaac taccacatt ttctaccaa 
      atgctcgcc ggcttgtcc gccgaagag cgcagcaaa 
      ctcaactta gaaggtcac acgccggcc actctgcgg 
      tacctgcaa cacggcgat acgcgccaa gacctggcc 
      gaggactcc tcgaggttt caaacgtgg aagacctgt 
      ttgggcatc cttggtatt ccgttcttg gatgttgtg 
      agggtccta gcggcggtg cttctcttg ggaaacgtg 
      caatttctg gacgggaag gggctggag gtggacgtg 
      aagggcgac acggagctg aatgccgtc gcggggctg 
      ctgggggtg tccggggct gcgctgttc cggggtctc 
      accactcgc acgcacaat gcgcgaggg cagctggtc 
      aagtcggtc tgcgacgcc aatatgtcg aacatgacg 
      cgggattgc ctggctaaa gcactgtat tgtcgaact 
      gtggccact atagttaga cgggccaac agtttgaag 
      aggttaggc tcgactctg gggactttg agttcggat 
      tcgaacgag tcggtgcat aatcaggca gaggttacg 
      agtcagcac gcttcgacg gtgaatggc agtaccaac 
      gctgggagc aagtccatg gcggcactt aacaacgcg 
      gtacgccat gccacggat gggtttatc gggattttg 
      gatatgttc gggtttgag gacccgaag cccagccag 
      ctggagcac ctctgcatc aatttatgc gcggagacg 
      atgcagcac ttctacaac acgcatatt ttcaagtcg 
      agtatcgag tcgtgtcgg gatgaggga ataaactgc 
      gaggtcgag gtggattac gttgataat gtcccttgt 
      atcgacttg atctcgtcg ctgcggacg ggtctgttg 
      agtatgctc gatgtggaa tgctcaatt cgcggcacc 
      gccgagtct tacgtatct aaaatcagc gtgcagcac 
      aaacacaat agtaggctc ttcgagccg aaaccggtc 
      gacccgcgc ctcttcggc atccagcac ttcgccggg 
      agggtcgtg tacgacgcc accgacttc ctggatacg 
      aacaaggac gtcgtcccc gatgacctg gtggcggtg 
      ttttacaaa cacaattgc aattttggg ttcgctact 
      catttattc ggcagcgaa ttgaaggct ttgtattcg 
      gtggagagc gtccctcga ggggtcagc ttccggata 
      tcgccgacg tcccacact gacctttta aatggcgac 
      gaacccgtg tctacactg acgcaagat ttccataca 
      cgactcgat aatttattg cgaacgctg gttcacgct 
      agacctcat ttcgtgcgc tgcattcgc agcaactca 
      caggagact cccaacaga ttcgatcgc ggaactgtt 
      gtccgacag atccgctcg ttacaagta ctcgaaacg 
      gttaatctc atggctgga ggatacccg catagaatg 
      cgcttcaaa gcgttcaat gccaggtac cggctcttg 
      gcccctttc gcgaggctg caccgcacg gacgagaaa 
      gtcaccgat gactgccat ctgatcctc caatacgtc 
      gtcgacttg atttccaaa cagcacaac acgttggtc 
      tcgacgagc tgggcgttg ggcaaacgc cacattttc 
      ctcagtgaa gggataaga caacacctg gaaaaactc 
      cggacggaa acgcgccag aaggcggca acgctgatt 
      caggccacc tggaggggt tggcattgc cggtaccga 
      tgggcggct ttacgccgc gagaaagag cacaaaagt 
      gccactaac accaacact ctgaataat cgtactcca 
      atagggggc gctatcgcc aggccacgg ccccaaccc 
      atcgccggg acgccccct cccgacccc aatgagaaa 
      tgcgacgcc aaaataatt caacagact tgcaatctc 
      tttgggctt gatttggaa cggccgcct cccgtgccc 
      cccagtcgt tcctacacc gtttcgggc aatacgaaa 
      cttggttac ccccaaact agagtaatg aaaatgtcg 
      taccccgag gattgcaac ggagaggga caagtgttg 
      atgaaagga gagactgtc ttagttgta ggagcttcg 
      caccgtcga ggtcatttg attgtcgaa cataaacac 
      tgtactttc cacgtacca ttccagttc ttggagttg 
      aaacaaagt gtcaatatt taa
    mRNA sequence
    • >TC032522 RA
      gtgcagatg cgggaaata ttcttctcc tcctagtca 
      ggtagcttt gagtcggag tgtgcacag tccccgtgt 
      gaagcactt aataaaaaa cgaagccta agtgaagac 
      cagtaaaga tttgagtcc cgaaaccag tttgtagcc 
      ctcgtcatg aatgcggcg gtctcgtgc tggacgcac 
      cattgaagt ctcctaatt gctccagcg acgagaagc 
      gttcgaaag ctcgccccc cggtcctcc tcatgtaaa 
      tgagcgagc gctggtcgc cgccatctc tcgagagct 
      agaggagcg gttagggcc tttgagacc cgtgatatc 
      ccgaaaagc tgcgtacag gtgtagcac aggtgagcg 
      gggagcatc acgtgagca ggtcgcagg tagcggccg 
      tcatccgag ccagtgcac cgaagtctt cgcgagtga 
      ttgttggtc gtcgtgttg tggtgtgat gtctgaaat 
      atggccggg catgctttc agcccctga aatatggct 
      acgctaggc ctctccaaa gtgttcatt ttggacaaa 
      tacttcacg gagctgcag aagttctgg gagacggag 
      aagaagctc caagatgcg tcgtcgtcg aacgaagcc 
      gtccacctg cagcagcgc ctccgcagc ctgagcacg 
      gagctggtg acgctccgc aaccgcctc catgtgcag 
      ggcgcgccg gcggccccc ctgaagggc gcccccgtg 
      ccggtggtg gccggcacg cagccggtg attcccccg 
      cggagcgcg ccgacggcg ccgcttccc gtggtggcc 
      gtccccgac agccgcccc cagctgccc accgcgggg 
      gccgacctg gacgacctc atccacctg caggggcct 
      ctcaccgaa gacgcggtg atgaagtgc ctccaggcg 
      cgattcaac gcttccaac ttctacaca aacgtgggt 
      ccaattctc ttgtgtgtg aacccatac agagacgtc 
      ggcaatcca ttaacactg aacagtact cgcggcctg 
      gccttgagc ccccaacta aacagggta gttcaggaa 
      gctgtccga cagcaatcc gaaacgggt tatccccaa 
      gccatcatc ctttcagga accagcggc tccggtaag 
      agccatgct tccatgttg cttctgcga caacttttt 
      gccgtcgct ggcggaggc cccgaaacg gacgctttc 
      aagcattta gccgcggct ttcacagta ttacggtcg 
      ctgggctcc gctaaaacc gccaccaac tcggagtcg 
      agccgaatt ggccagttc atcgaagtg caggtcacg 
      gacggggcg ctgtaccgc accaaaatc cactgctac 
      tttctggac cagacgcgc gtcatccgg cccttgccc 
      ggcgagaaa aactaccac attttctac caaatgctc 
      gccggcttg tccgccgaa gagcgcagc aaactcaac 
      ttagaaggt cacacgccg gccactctg cggtacctg 
      caacacggc gatacgcgc caagacctg gccgaggac 
      tcctcgagg tttcaaacg tggaagacc tgtttgggc 
      atccttggt attccgttc ttggatgtt gtgagggtc 
      ctagcggcg gtgcttctc ttgggaaac gtgcaattt 
      ctggacggg aaggggctg gaggtggac gtgaagggc 
      gacacggag ctgaatgcc gtcgcgggg ctgctgggg 
      gtgtccggg gctgcgctg ttccggggt ctcaccact 
      cgcacgcac aatgcgcga gggcagctg gtcaagtcg 
      gtctgcgac gccaatatg tcgaacatg acgcgggat 
      tgcctggct aaagcactg tattgtcga actgtggcc 
      actatagtt agacgggcc aacagtttg aagaggtta 
      ggctcgact ctggggact ttgagttcg gattcgaac 
      gagtcggtg cataatcag gcagaggtt acgagtcag 
      cacgcttcg acggtgaat ggcagtacc aacgctggg 
      agcaagtcc atggcggca cttaacaac gcggtacgc 
      catgccacg gatgggttt atcgggatt ttggatatg 
      ttcgggttt gaggacccg aagcccagc cagctggag 
      cacctctgc atcaattta tgcgcggag acgatgcag 
      cacttctac aacacgcat attttcaag tcgagtatc 
      gagtcgtgt cgggatgag ggaataaac tgcgaggtc 
      gaggtggat tacgttgat aatgtccct tgtatcgac 
      ttgatctcg tcgctgcgg acgggtctg ttgagtatg 
      ctcgatgtg gaatgctca attcgcggc accgccgag 
      tcttacgta tctaaaatc agcgtgcag cacaaacac 
      aatagtagg ctcttcgag ccgaaaccg gtcgacccg 
      cgcctcttc ggcatccag cacttcgcc gggagggtc 
      gtgtacgac gccaccgac ttcctggat acgaacaag 
      gacgtcgtc cccgatgac ctggtggcg gtgttttac 
      aaacacaat tgcaatttt gggttcgct actcattta 
      ttcggcagc gaattgaag gctttgtat tcggtggag 
      agcgtccct cgaggggtc agcttccgg atatcgccg 
      acgtcccac actgacctt ttaaatggc gacgaaccc 
      gtgtctaca ctgacgcaa gatttccat acacgactc 
      gataattta ttgcgaacg ctggttcac gctagacct 
      catttcgtg cgctgcatt cgcagcaac tcacaggag 
      actcccaac agattcgat cgcggaact gttgtccga 
      cagatccgc tcgttacaa gtactcgaa acggttaat 
      ctcatggct ggaggatac ccgcataga atgcgcttc 
      aaagcgttc aatgccagg taccggctc ttggcccct 
      ttcgcgagg ctgcaccgc acggacgag aaagtcacc 
      gatgactgc catctgatc ctccaatac gtcgtcgac 
      ttgatttcc aaacagcac aacacgttg gtctcgacg 
      agctgggcg ttgggcaaa cgccacatt ttcctcagt 
      gaagggata agacaacac ctggaaaaa ctccggacg 
      gaaacgcgc cagaaggcg gcaacgctg attcaggcc 
      acctggagg ggttggcat tgccggtac cgatgggcg 
      gctttacgc cgcgagaaa gagcacaaa agtgccact 
      aacaccaac actctgaat aatcgtact ccaataggg 
      ggcgctatc gccaggcca cggccccaa cccatcgcc 
      gggacgccc cctcccgac cccaatgag aaatgcgac 
      gccaaaata attcaacag acttgcaat ctctttggg 
      cttgatttg gaacggccg cctcccgtg ccccccagt 
      cgttcctac accgtttcg ggcaatacg aaacttggt 
      tacccccaa actagagta atgaaaatg tcgtacccc 
      gaggattgc aacggagag ggacaagtg ttgatgaaa 
      ggagagact gtcttagtt gtaggagct tcgcaccgt 
      cgaggtcat ttgattgtc gaacataaa cactgtact 
      ttccacgta ccattccag ttcttggag ttgaaacaa 
      agtgtcaat atttaaact tgccatttt atcgtattc 
      taagttgta aaatagtag gtttagata aatatgttt 
      tatttccat acttattga gatgtgttc attgtatag 
      cgaaaatgc cgccacagc cataatttt gctggtact 
      cttgattgt aattatttg tcataagac accaacgat 
      ttgtacaaa ccgtaattt aaatataca taaaactgt 
      cct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY