Status
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Gene information
General information
TC010519 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 14532849..14538764
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010519
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01704

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010519 RA
      MSRRRATEE TDKLTRIAI VNADKCKPK RCRQECKKS 
      CPVVRMGKL CIEVTPNSK MAAISEELC IGCGICVKK 
      CPFEAITII NLPSNLERD TTHRYGKNS FKLHRLPIP 
      RPGEVLGLV GTNGIGKST ALKILAGKQ KPNLGRYSD 
      PPDWTEILS HFRGSELQN YFTKILEDD LKALIKPQY 
      VDQIPKAVK GTVGQLLDK KTELDNQEQ ICEMLDLKH 
      IRDRAIEAL SGGELQRFA CAMVCIQNG DIFMFDEPS 
      SYLDVKQRL NAAQTIRSL LHPDKFIIV VEHDLSVLD 
      YLSDFICCL YGVPGAYGV VTMPFSVRE GINIFLDGF 
      VPTENLRFR DESLVFKVA ESANEEEIK RMNHYEYPS 
      MTKTMGNFQ LNVVKGQFS DSEILVLLG ENGTGKTTF 
      IRMLAGNLQ ADDGSGELP QLHISYKPQ KISPKSQGL 
      VRQLLHEKI RDAYIHPQF MADVTKPLK IDDIIDQEV 
      QNLSGGELQ RVALTLCLG KPADVYLID EPSAYLDSE 
      QRLMAAKVI KRFILHAKK TGFVVEHDF IMATYLADR 
      VIVFEGTPS VKTTANSPQ TLLAGMNRF LELLGITFR 
      RDPNNFRPR INKQESVKD VEQKRSGQY FFLED
    CDS sequence
    • >TC010519 RA
      atgtcacga agaagggca accgaagaa acagacaaa 
      ttaactcgt atcgctatt gtgaacgct gataagtgt 
      aagccgaaa cgatgtcgc caggagtgc aaaaagtcg 
      tgccctgtg gtccgcatg ggcaagtta tgtatcgaa 
      gtgaccccc aacagcaaa atggcagca atttccgag 
      gaactgtgc attgggtgc ggtatctgt gtgaagaaa 
      tgccccttt gaagccatc actatcatc aatcttccc 
      agtaatttg gagagagat actactcat cgctatggc 
      aaaaactcc ttcaaactt cacagattg ccaataccc 
      agacccggc gaagttcta ggtcttgtg gggaccaac 
      ggtattggg aaatccacc gcgttgaaa atccttgca 
      gggaagcaa aaacccaat ttggggcgc tactctgac 
      cctccagac tggacggag atcctgagc catttcagg 
      gggtctgag ctgcagaat tacttcact aagatattg 
      gaagacgac ctgaaagcg ctaatcaaa ccacaatac 
      gttgatcaa attcctaaa gctgtaaaa ggaacggtt 
      ggacaactt ttggacaag aagaccgag ttggataac 
      caggaacag atttgtgag atgttggat ttgaagcat 
      atacgcgac cgagcgatt gaggcgctc tctggtggg 
      gagttgcag agatttgcc tgtgctatg gtgtgcatt 
      caaaacggg gatattttc atgtttgac gagccttca 
      tcgtattta gatgtaaaa cagagatta aatgctgcc 
      caaactata agatcgttg ttacatcca gacaaattt 
      ataattgtc gtggaacac gatctcagc gtcttagat 
      tatttatca gatttcatt tgttgtttg tatggtgtt 
      cctggagcg tatggtgtg gttactatg ccattctcc 
      gttcgtgaa ggaattaat atatttttg gacggtttt 
      gttccaacc gaaaatctg cgtttccgt gacgaatct 
      ttagtgttc aaagtggct gaatctgcc aatgaagag 
      gaaataaaa cgtatgaac cattacgag tacccttcg 
      atgactaaa accatggga aatttccaa ctaaacgtg 
      gtcaaaggc caattttcc gattccgaa attttggtt 
      ttactcggc gaaaacggc accggtaaa acaacattc 
      attcgaatg ttggccggc aatttacaa gccgatgat 
      ggttccggg gaattacca caacttcat atcagttac 
      aagccgcaa aagatcagt ccgaaatct cagggatta 
      gtccgacaa ttgttacac gagaaaatc agggacgct 
      tacatccac ccacagttt atggctgac gtcactaag 
      ccgcttaaa atcgacgat attatcgac caagaggtg 
      caaaatctg tctggtggt gagttgcaa cgtgtggct 
      ctaactctg tgtttgggc aaaccagct gatgtctac 
      cttatcgac gaacccagt gcctactta gattccgag 
      cagcgtctc atggctgct aaagtaatc aaaagattt 
      attttgcat gcgaagaag acaggtttt gtggtagaa 
      cacgacttc atcatggct acttacctg gcagaccgc 
      gtgattgta tttgagggt acgccatct gttaagacc 
      acagccaac tcgccacag actcttcta gctggaatg 
      aaccgattt ttggagttg ttagggatt acatttagg 
      cgggatcct aataatttt agacccaga ataaacaag 
      caagagtct gttaaggat gttgagcag aaacggtca 
      ggacagtat ttctttttg gaggattaa
    mRNA sequence
    • >TC010519 RA
      atcctttcc tcatggccg gtcgtgctg gcggcttag 
      aataaattt gttttacat aaattcaaa ttactggtt 
      aggtgtcac tgagtccta acatattga gtcatgtca 
      cgaagaagg gcaaccgaa gaaacagac aaattaact 
      cgtatcgct attgtgaac gctgataag tgtaagccg 
      aaacgatgt cgccaggag tgcaaaaag tcgtgccct 
      gtggtccgc atgggcaag ttatgtatc gaagtgacc 
      cccaacagc aaaatggca gcaatttcc gaggaactg 
      tgcattggg tgcggtatc tgtgtgaag aaatgcccc 
      tttgaagcc atcactatc atcaatctt cccagtaat 
      ttggagaga gatactact catcgctat ggcaaaaac 
      tccttcaaa cttcacaga ttgccaata cccagaccc 
      ggcgaagtt ctaggtctt gtggggacc aacggtatt 
      gggaaatcc accgcgttg aaaatcctt gcagggaag 
      caaaaaccc aatttgggg cgctactct gaccctcca 
      gactggacg gagatcctg agccatttc agggggtct 
      gagctgcag aattacttc actaagata ttggaagac 
      gacctgaaa gcgctaatc aaaccacaa tacgttgat 
      caaattcct aaagctgta aaaggaacg gttggacaa 
      cttttggac aagaagacc gagttggat aaccaggaa 
      cagatttgt gagatgttg gatttgaag catatacgc 
      gaccgagcg attgaggcg ctctctggt ggggagttg 
      cagagattt gcctgtgct atggtgtgc attcaaaac 
      ggggatatt ttcatgttt gacgagcct tcatcgtat 
      ttagatgta aaacagaga ttaaatgct gcccaaact 
      ataagatcg ttgttacat ccagacaaa tttataatt 
      gtcgtggaa cacgatctc agcgtctta gattattta 
      tcagatttc atttgttgt ttgtatggt gttcctgga 
      gcgtatggt gtggttact atgccattc tccgttcgt 
      gaaggaatt aatatattt ttggacggt tttgttcca 
      accgaaaat ctgcgtttc cgtgacgaa tctttagtg 
      ttcaaagtg gctgaatct gccaatgaa gaggaaata 
      aaacgtatg aaccattac gagtaccct tcgatgact 
      aaaaccatg ggaaatttc caactaaac gtggtcaaa 
      ggccaattt tccgattcc gaaattttg gttttactc 
      ggcgaaaac ggcaccggt aaaacaaca ttcattcga 
      atgttggcc ggcaattta caagccgat gatggttcc 
      ggggaatta ccacaactt catatcagt tacaagccg 
      caaaagatc agtccgaaa tctcaggga ttagtccga 
      caattgtta cacgagaaa atcagggac gcttacatc 
      cacccacag tttatggct gacgtcact aagccgctt 
      aaaatcgac gatattatc gaccaagag gtgcaaaat 
      ctgtctggt ggtgagttg caacgtgtg gctctaact 
      ctgtgtttg ggcaaacca gctgatgtc taccttatc 
      gacgaaccc agtgcctac ttagattcc gagcagcgt 
      ctcatggct gctaaagta atcaaaaga tttattttg 
      catgcgaag aagacaggt tttgtggta gaacacgac 
      ttcatcatg gctacttac ctggcagac cgcgtgatt 
      gtatttgag ggtacgcca tctgttaag accacagcc 
      aactcgcca cagactctt ctagctgga atgaaccga 
      tttttggag ttgttaggg attacattt aggcgggat 
      cctaataat tttagaccc agaataaac aagcaagag 
      tctgttaag gatgttgag cagaaacgg tcaggacag 
      tatttcttt ttggaggat taaattttg ttatgaaat 
      attgtgtat tatatggcg ctctttaca ggtttctgt 
      ttttataaa caaatataa tatatgtgg aaggataat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY