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Gene information
General information
TC010495 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid19: 1217678..1258479
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010495
    iBeetle screen iB_01703
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    tropharium shape irregular - Number of animals showing the phenotype: 4

    egg chamber number decreased - Number of animals showing the phenotype: 4

    mature egg not present - Number of animals showing the phenotype: 4
    tropharium shortened - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010495 RA
      MQRLHPGKG TTSRQNPFV SLSEQLHSS ISFSKHKAN 
      RKMVERILE EAENYDPEC NGDVTNGDS IGGCTGGGG 
      GSASDSGDQ MDADARAKE LAHRKEEAK RKRRKKKRT 
      GSSLVSSCF QDLYKLSGE ILGEGAYAS VQTCINIYT 
      DQEYAVKII DKVPGHARA RVFREVETF HHCQGHPNI 
      IQLTEFFED EEKFYLVFE KINGGQLLR RIQENRYFS 
      EAAAAEIVR EVASALQFM HAKGIAHRD LKPENILCV 
      NKDTICPIK ICDLDLGSG IRFQSSVSS PLATPQLLT 
      PVGSAEFMA PEVVEAFIG ESDSVAYDK RCDLWSLGV 
      ITYILLCGY PPFYGKCGR DCGWERGDN CNACQDLLF 
      HSIQEGRYD FPEAEWGNI SDEAKDLIS SLLVKNASE 
      RIRAEEVLQ HPWLKCASE SATLTTPAI IKKNNSARE 
      LSQFAESAM AVNRVVQQH FSMKLDYLE RPNVYPIDE 
      KAAPYHAKV FGLSPPSES NLMQRRIKG RSCQFLLPT 
      PAPAIPSPS G
    CDS sequence
    • >TC010495 RA
      atgcagcgg ctacatcca ggaaaggga actactagt 
      cggcagaat ccctttgtt tctctttcg gaacaactt 
      cattcttca atttcgttc agcaaacat aaggcaaac 
      cgaaaaatg gtcgaaagg atattagaa gaagcggaa 
      aactacgat ccagaatgc aatggcgac gttaccaat 
      ggcgacagc atcgggggc tgcacgggc ggtggtgga 
      ggttctgcc tccgatagt ggcgaccag atggacgcc 
      gatgcccga gccaaagaa ctagcccat cgcaaggag 
      gaggccaaa aggaaacgc cgcaaaaag aaacgcacc 
      ggctcgtcg ctagtttca tcgtgtttc caagatttg 
      tataaactt tctggcgaa atcttgggt gagggggcc 
      tatgccagt gttcaaacc tgcatcaat atttacacc 
      gatcaagaa tatgcagtc aaaatcatc gataaggta 
      ccgggtcat gccagggct cgggtgttc agagaagtt 
      gaaactttc catcattgt caaggccac cccaatatc 
      attcagctc accgagttt ttcgaggat gaagaaaag 
      ttttatttg gtttttgag aagataaat ggaggacag 
      ctgttgagg aggattcag gagaatagg tatttcagt 
      gaagctgca gcagctgaa atagtacga gaagttgct 
      tcagcattg caattcatg catgccaaa ggcatagcc 
      caccgtgat ctcaaacct gaaaacatc ttgtgtgtg 
      aataaggat actatttgt ccaattaaa atttgtgat 
      ttggatttg ggatccggg attcgcttc cagtccagt 
      gtttcgagt ccgctggca actccacaa cttttgact 
      ccggtgggc agcgctgag ttcatggcg cccgaagtt 
      gtggaggcc ttcatcggc gagtcggac tcggtggcg 
      tacgacaaa cgttgcgat ttatggtcg ctgggcgtc 
      ataacgtac attcttttg tgcggttat ccgccattt 
      tacggcaaa tgcggtcgt gactgcggt tgggaacga 
      ggcgacaat tgcaatgct tgccaagat ctactcttc 
      cacagtatc caagagggc cgatacgac ttccccgaa 
      gccgaatgg ggtaacata tcggacgag gcaaaggat 
      ctgatatca tccttgctg gtgaaaaat gccagcgaa 
      cgcatcaga gccgaggaa gtgctccaa catccgtgg 
      ttgaaatgc gctagcgaa tcggcgact ttgaccacg 
      cccgccatc atcaagaag aacaattcc gcgagagaa 
      ctatcgcaa ttcgcggag agcgctatg gctgtgaac 
      agggtggtg cagcaacac ttcagcatg aagctggac 
      tatctggag aggccgaat gtctatccg attgacgag 
      aaggcggcg ccttaccac gccaaggtg tttggcttg 
      agtccgcct agcgagagc aacctaatg cagaggcgg 
      atcaaaggt cgatcgtgc cagttcttg ctgcccact 
      ccggccccc gccattccc agtccaagc ggttga
    mRNA sequence
    • >TC010495 RA
      ctctgaagg taaagaaga agcagagta aagaagaaa 
      aagaggaat acaaaaaac tagatgggt aattaactg 
      tacaaaccg taacttttt taacttatg aaacgattt 
      ttaaatcac aagttttac ctcctttct tgtgcgcac 
      gcgccaaat gtagtttgt tgtgggtaa gatggcggg 
      cctgaaagt gttgtctga gtcggaggc agactgcac 
      taatcaagc caaaaggta tgcagcggc tacatccag 
      gaaagggaa ctactagtc ggcagaatc cctttgttt 
      ctctttcgg aacaacttc attcttcaa tttcgttca 
      gcaaacata aggcaaacc gaaaaatgg tcgaaagga 
      tattagaag aagcggaaa actacgatc cagaatgca 
      atggcgacg ttaccaatg gcgacagca tcgggggct 
      gcacgggcg gtggtggag gttctgcct ccgatagtg 
      gcgaccaga tggacgccg atgcccgag ccaaagaac 
      tagcccatc gcaaggagg aggccaaaa ggaaacgcc 
      gcaaaaaga aacgcaccg gctcgtcgc tagtttcat 
      cgtgtttcc aagatttgt ataaacttt ctggcgaaa 
      tcttgggtg agggggcct atgccagtg ttcaaacct 
      gcatcaata tttacaccg atcaagaat atgcagtca 
      aaatcatcg ataaggtac cgggtcatg ccagggctc 
      gggtgttca gagaagttg aaactttcc atcattgtc 
      aaggccacc ccaatatca ttcagctca ccgagtttt 
      tcgaggatg aagaaaagt tttatttgg tttttgaga 
      agataaatg gaggacagc tgttgagga ggattcagg 
      agaataggt atttcagtg aagctgcag cagctgaaa 
      tagtacgag aagttgctt cagcattgc aattcatgc 
      atgccaaag gcatagccc accgtgatc tcaaacctg 
      aaaacatct tgtgtgtga ataaggata ctatttgtc 
      caattaaaa tttgtgatt tggatttgg gatccggga 
      ttcgcttcc agtccagtg tttcgagtc cgctggcaa 
      ctccacaac ttttgactc cggtgggca gcgctgagt 
      tcatggcgc ccgaagttg tggaggcct tcatcggcg 
      agtcggact cggtggcgt acgacaaac gttgcgatt 
      tatggtcgc tgggcgtca taacgtaca ttcttttgt 
      gcggttatc cgccatttt acggcaaat gcggtcgtg 
      actgcggtt gggaacgag gcgacaatt gcaatgctt 
      gccaagatc tactcttcc acagtatcc aagagggcc 
      gatacgact tccccgaag ccgaatggg gtaacatat 
      cggacgagg caaaggatc tgatatcat ccttgctgg 
      tgaaaaatg ccagcgaac gcatcagag ccgaggaag 
      tgctccaac atccgtggt tgaaatgcg ctagcgaat 
      cggcgactt tgaccacgc ccgccatca tcaagaaga 
      acaattccg cgagagaac tatcgcaat tcgcggaga 
      gcgctatgg ctgtgaaca gggtggtgc agcaacact 
      tcagcatga agctggact atctggaga ggccgaatg 
      tctatccga ttgacgaga aggcggcgc cttaccacg 
      ccaaggtgt ttggcttga gtccgccta gcgagagca 
      acctaatgc agaggcgga tcaaaggtc gatcgtgcc 
      agttcttgc tgcccactc cggcccccg ccattccca 
      gtccaagcg gttgaagac gtgttgcgc aagcatctc 
      caatatttt tttacaaaa taaggaaaa aaaagagaa 
      aaagtgcac gcgcaggag gcgcgtgtt ttgttactt 
      tcgttcaat tttttcgtc agaatattc ttcttcgtt 
      ttcatcatg agtcttttg actgtgtca ggacgtcgt 
      ctgcgtacg caaatacgc agaatgtcg tcttggcac 
      agttgtggc gtctccggc ctagtgtta gtcttttgg 
      agattgttt tccaatttt tttttcttt tttttttgt 
      atgtgttga ggtggactg attgcggtc agtccgcat 
      tgtgataat taaataagc atctaagcg tgcgaagta 
      gcgcacgat agactgtag acagcgtac ctgctttat 
      gttcggtaa gatacagaa cattaattc gagggacgg 
      ccgtcccac catgtactg acagtccat tgtatttta 
      agagtccta gtaagttcg gatattttt taaaagatt 
      ttgtaatgc tcaagggta gtttccggt attcgctag 
      taaggtgcg caattttcg gtcagtccc agtgaaaaa 
      aaaaagttt ttgatggcc aaaaattct gcatcatct 
      tccaacact cagggttgt tcattaaat ggcgagtca 
      ttacaaaac ttatatcag agaagggct cgtaacgcc 
      agaatgaat gtcattagt ttgtatgtg atatagggt 
      gaaagatat ccccaggag aagatccga catgattgg 
      aggagtatt cgtttttct atgcttatt tttatggca 
      tttattatg tacgaaagt acgaaatat tgtattttc 
      caatcagtt gataccaaa aatatgatg aaaaattta 
      taaaaataa aatatttaa gtcttaatt ttcatccag 
      atttcttta ataccttta tcagcactg caagtgcca 
      tcgtttgtt tttctttat atttgtgtg ttgtaagtg 
      catcggtat gagaacacc gtactttgt ttttccttc 
      tcatgccgg cgcaattat aatgcagtt ttcagatca 
      atttataat gttaattaa tgacgtatt ttttttaca 
      accaagcaa gctagagtt gaataaatt agtgatgga 
      aatttatgt accaagtgt gctttaatt tcatataat 
      aaagaccga ttttttta