General information | |||
TC010491 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 15695564..15709437 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC010491 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01702 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Comment: Due to the lack of GFP-Signal, status of tissue, eg. presence of muscles, is not scorable. |
Comment: Majority of animals still lays in the egg shell. If outside they appear in an u-shape, with the head bent towards the abdomen. All fluorescence is missing, musculature as well as nervous system and eyes. |
gena triplet seta number increased - Penetrance (percentage of animals/eggs on slide): more than 80%
gena triplet seta pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
thorax bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC010491 RA
MLRLSNSVR NSCDRFIPL RLNDEISPD PSNFDPLDH EVLENTGFI NYAKIRYNR NNYKNALRS ALLPYEPKV LNFFPPKKF KVKKTPGME HTWPVKPRE KPVIGLPKT VLDMPQFDS TIFHHVCDW SSEGCIATI FQNEVHIWN HVTQRRIRI TSTTHRIEY SIKWKNGGT QFAVGLFPS SVGIWDVVA NKLICWESC GHYAHKCKV FALEWINAQ LLVTGCSLG SLKVFNSEL NKIRESTFA HTGAILTIV RSCDGRFIA TTGMDKMMI VWRIPTLEK HFTFETDTI SRAVAWHPW RGSLLAVGV GQRNGHIVI FNINKKSSI AEEKFTYRN ATVNSLTFN PISGELVAS YFVRDARDR RKGVGVVVV FSNIDRIEE ELHLHDGPI LYLLWGNKG RNLASASAD ENLCIWDFF GKMDEQSQN GRKKKEKNT PTSSVLRST IR
- >TC010491 RA
atgctgcgt ttgtccaat tctgtaaga aactcttgt gatagattt attccgctt cgcctcaac gacgaaata tcaccagac ccatcaaac ttcgatccc ttagaccac gaagtattg gaaaacacc ggctttatc aattatgcc aaaatccga tacaaccgc aacaactac aaaaacgcg ttacgaagc gctttatta ccgtacgaa ccgaaagtt ttgaatttt tttccaccg aaaaaattc aaagtgaag aaaacaccg ggcatggaa catacttgg ccggttaaa ccgagggaa aagccggtc attggcttg ccgaagact gtcctagac atgccccaa ttcgattcc actatcttt caccatgtg tgtgattgg agcagtgaa ggctgtatt gccaccatt ttccaaaac gaggttcac atatggaac catgttacg cagagaaga ataagaatt acgtctaca actcatcga atagagtac tccatcaag tggaagaat ggagggacg cagtttgcg gtcggtttg ttccccagc agtgtcgga atttgggat gtagtagca aacaagctc atttgttgg gaatcctgt ggccactac gcccacaag tgcaaagtc tttgccctc gagtggata aatgcccaa cttttggtc accggatgt tctctagga tctttgaaa gtcttcaat tctgagcta aacaaaatc agggagtcg acttttgct cacactggt gccatttta accatcgta agatcttgc gatggcaga tttattgca acgactgga atggataaa atgatgatt gtgtggaga attccaact ttggagaaa catttcacg tttgaaacg gacacaatt agccgggca gtggcatgg cacccctgg cgtggctct cttctggca gtaggagta ggtcaaagg aatggacac atagtcatt tttaatata aataagaaa agttctatt gcggaggag aaatttact tatcgcaat gctacagtc aattctctc accttcaat ccaatcagt ggggagctt gtggcgagt tatttcgtc cgcgatgcg agagacaga cgcaagggg gtcggagtt gtggtcgtt ttctcaaat attgaccga atagaagag gaactgcat ctccacgat ggtccgatt ttgtatcta ttgtgggga aacaaaggt cggaattta gcgtcggca agtgccgac gagaatttg tgtatttgg gattttttc ggaaaaatg gatgaacaa agccaaaac ggaaggaag aaaaaagaa aaaaataca cctacaagt tcggtacta cgttctact attagataa
- >TC010491 RA
ttgcacaaa tttttagat ttttgtttt cagttttta tgaacaata tttatcatt tgtgtcgtg tctctcgtt tgaaaattt taaaaatgc tgcgtttgt ccaattctg taagaaact cttgtgata gatttattc cgcttcgcc tcaacgacg aaatatcac cagacccat caaacttcg atcccttag accacgaag tattggaaa acaccggct ttatcaatt atgccaaaa tccgataca accgcaaca actacaaaa acgcgttac gaagcgctt tattaccgt acgaaccga aagttttga atttttttc caccgaaaa aattcaaag tgaagaaaa caccgggca tggaacata cttggccgg ttaaaccga gggaaaagc cggtcattg gcttgccga agactgtcc tagacatgc cccaattcg attccacta tctttcacc atgtgtgtg attggagca gtgaaggct gtattgcca ccattttcc aaaacgagg ttcacatat ggaaccatg ttacgcaga gaagaataa gaattacgt ctacaactc atcgaatag agtactcca tcaagtgga agaatggag ggacgcagt ttgcggtcg gtttgttcc ccagcagtg tcggaattt gggatgtag tagcaaaca agctcattt gttgggaat cctgtggcc actacgccc acaagtgca aagtctttg ccctcgagt ggataaatg cccaacttt tggtcaccg gatgttctc taggatctt tgaaagtct tcaattctg agctaaaca aaatcaggg agtcgactt ttgctcaca ctggtgcca ttttaacca tcgtaagat cttgcgatg gcagattta ttgcaacga ctggaatgg ataaaatga tgattgtgt ggagaattc caactttgg agaaacatt tcacgtttg aaacggaca caattagcc gggcagtgg catggcacc cctggcgtg gctctcttc tggcagtag gagtaggtc aaaggaatg gacacatag tcattttta atataaata agaaaagtt ctattgcgg aggagaaat ttacttatc gcaatgcta cagtcaatt ctctcacct tcaatccaa tcagtgggg agcttgtgg cgagttatt tcgtccgcg atgcgagag acagacgca agggggtcg gagttgtgg tcgttttct caaatattg accgaatag aagaggaac tgcatctcc acgatggtc cgattttgt atctattgt ggggaaaca aaggtcgga atttagcgt cggcaagtg ccgacgaga atttgtgta tttgggatt ttttcggaa aaatggatg aacaaagcc aaaacggaa ggaagaaaa aagaaaaaa atacaccta caagttcgg tactacgtt ctactatta gataatttt aatgtgttt gtttgtgat tttaaatta tcaaaagcg ggtcttaag tacagtttt ataatttta catttgaat aaattttta caagagtt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
