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Gene information
General information
TC010489 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid19: 1322703..1372879
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010489
    iBeetle screen iB_01701
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    leg musculature not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    leg musculature sometimes rounded - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs pretarsus shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs pretarsus rounded - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010489 RA
      MDGTQRYLL IATAALFLF FDLCHGVRA PGAKKKVGP 
      IDQSAAAAS WHDFDNSDF FQSEHAVIQ THPSIGGGP 
      RFSSGGGRK AWKHLDFRN SATAELLKN PSLSSPDEC 
      ARACREGEP PRICYYHFT LELYTVLGA ACQVCTPNA 
      TNTVWSHCQ CVLADGVER GILTANRMI PGPSIQVCE 
      GDKVVIDVE NHIEGNEVT LHWHGVWQR GSQYYDGVP 
      FVTQCPIQQ GNTFRYQWI AGNAGTHFW HAHTGLQKM 
      DGLYGSVVI RQPPAKDPN SHLYDYDLT THVMLLSDW 
      MHEDATERF PGRLAVNTG QDPESLLIN GKGQFRDPN 
      TGFMTNTPL EVFTITPGR RYRFRMINS FASVCPAQL 
      TIQGHDLTL IATDGEPVH PVRVNTIIS FSGERYDFV 
      INADQTPGA YWIQLRGLG ECGIRRVQQ LGILRYAKG 
      PYQPSQAPP TYDYGIPQG VVLNPLDAR CNEIRPDAI 
      CVSQLKNAL SIDKGILRE KPDVKIFLP FRFHIYTPE 
      DLFAPNTYN RHLVAPNGD HVISLIDEI SYMAPPAPL 
      ISQYDDIDP QQFCNGDNR PADCQQNCM CTHKVDIPL 
      NAIVEIVLV DEVQQPNLS HPFHLHGYA FNVIGIGRS 
      PDQNVKKIN LKHALDLDR QGLLHRQFN LPPAKDTIA 
      VPNNGYVVL RLRANNPGF WLFHCHFLF HIVIGMNLV 
      LQVGTHADL PPVPPNFPT CGDHVPEIN SNPNLV
    CDS sequence
    • >TC010489 RA
      atggacgga acacaacgg taccttctg atcgcgacc 
      gcggcactc ttcctcttc tttgacctc tgccatgga 
      gtgcgagca ccaggcgcc aagaagaaa gtgggacca 
      atagaccag tctgcagcc gctgcgtct tggcatgat 
      ttcgacaac tcagatttt ttccaatct gaacatgct 
      gtgatccaa actcacccc tcgatagga ggagggccc 
      agatttagt tcagggggt gggagaaag gcgtggaaa 
      catttagat tttcgaaat tcggcgact gctgaatta 
      ttaaaaaat ccatcactg tcgtcacct gatgaatgt 
      gcgagggct tgtcgagaa ggggaaccc ccaagaatt 
      tgctactat catttcacg ctggaattg tacaccgtg 
      cttggagca gcctgccaa gtgtgtaca cctaacgcc 
      accaacacc gtctggtcc cattgtcaa tgcgtctta 
      gccgatggt gtcgaaaga ggcattcta acagcaaac 
      cgtatgatc ccagggccc agcatccaa gtctgcgaa 
      ggtgacaag gttgtgatc gacgtcgag aaccacatc 
      gaaggcaac gaagtgacc ctccactgg cacggagtc 
      tggcaaaga ggaagtcaa tactacgat ggagtgcct 
      ttcgttacc caatgcccc attcagcaa ggaaacacc 
      ttcaggtac caatggata gccggaaac gctggcact 
      cacttctgg cacgctcac accggttta caaaaaatg 
      gacggtctt tacggcagt gttgtgatc cgtcaacca 
      ccagccaag gaccccaac agtcactta tacgactat 
      gatttgacc acacacgtc atgcttttg agtgactgg 
      atgcacgaa gacgctact gaaagattc cccggaaga 
      ttggctgtc aacactgga caagatcct gagagtttg 
      ctcatcaac ggaaaagga caattcaga gatcccaat 
      actgggttt atgaccaat actccactt gaagtcttt 
      accattacg cctggacga agatacaga tttaggatg 
      attaattcg ttcgcttca gtgtgtcca gctcagttg 
      acgattcaa ggacatgat ttgacgctt attgctacc 
      gatggagag ccagtgcat cctgtgagg gttaacaca 
      atcatctcg ttttcaggt gaacgatac gattttgta 
      atcaacgct gaccaaaca cccggtgct tattggatc 
      caacttcga ggcttaggg gagtgtggc ataagaaga 
      gtacaacag ttgggtatt ctgcggtat gccaaaggg 
      ccataccaa cccagccaa gccccgccc acttatgac 
      tacggtatt cctcaagga gtggtttta aatcctttg 
      gacgcaaga tgtaatgaa attcgacca gacgccatc 
      tgtgtaagc cagttaaag aatgccctc agcattgat 
      aagggtatt ctgcgcgaa aaaccagac gttaaaatt 
      ttcttaccc ttccgtttc catatctac acgcctgag 
      gatcttttc gcccccaat acatacaac agacattta 
      gttgcacca aatggcgac cacgtcata agtttaatc 
      gacgaaatt tcctacatg gccccacct gcacctctt 
      atatctcaa tatgacgat atcgacccc caacaattc 
      tgcaacggt gataaccga ccagccgat tgccaacaa 
      aactgcatg tgcacccac aaagttgac attccactc 
      aacgccatc gtcgaaatc gtactagtt gacgaagta 
      caacaacca aatctaagc catccattc cacttgcac 
      ggctacgcc ttcaacgtg atcggcatt gggcgatct 
      cctgaccaa aacgtgaag aaaatcaac ttgaagcat 
      gcgctagat ctggacaga caagggctt ctccaccgt 
      caattcaac cttccgcca gctaaagac acgatagct 
      gtgccaaac aacggatac gtggtgtta cgacttagg 
      gcaaacaat cccggattc tggctgttc cattgtcac 
      ttcctcttc cacattgtg ataggaatg aatctagtt 
      ttgcaagtt ggcacccat gccgatctg ccgccggtt 
      cctcccaat ttccccaca tgtggagac cacgtgcca 
      gaaattaac tcgaatcct aacctcgtt taa
    mRNA sequence
    • >TC010489 RA
      agatggtcc gctgatttt gttttgttt aaaaaaacg 
      aaggcactc tctttaaaa ccaagtggt ggagcacaa 
      ggttttata aagtccggt gaacgcttg cggttgtgg 
      taaacgcgc tgtggtaaa aatagggca gtgcgaaag 
      agtgattgt gcgagaacg gacggattg ctgtttggt 
      tagtagtgt ttgcttgta acatggacg gaacacaac 
      ggtaccttc tgatcgcga ccgcggcac tcttcctct 
      tctttgacc tctgccatg gagtgcgag caccaggcg 
      ccaagaaga aagtgggac caatagacc agtctgcag 
      ccgctgcgt cttggcatg atttcgaca actcagatt 
      ttttccaat ctgaacatg ctgtgatcc aaactcacc 
      cctcgatag gaggagggc ccagattta gttcagggg 
      gtgggagaa aggcgtgga aacatttag attttcgaa 
      attcggcga ctgctgaat tattaaaaa atccatcac 
      tgtcgtcac ctgatgaat gtgcgaggg cttgtcgag 
      aaggggaac ccccaagaa tttgctact atcatttca 
      cgctggaat tgtacaccg tgcttggag cagcctgcc 
      aagtgtgta cacctaacg ccaccaaca ccgtctggt 
      cccattgtc aatgcgtct tagccgatg gtgtcgaaa 
      gaggcattc taacagcaa accgtatga tcccagggc 
      ccagcatcc aagtctgcg aaggtgaca aggttgtga 
      tcgacgtcg agaaccaca tcgaaggca acgaagtga 
      ccctccact ggcacggag tctggcaaa gaggaagtc 
      aatactacg atggagtgc ctttcgtta cccaatgcc 
      ccattcagc aaggaaaca ccttcaggt accaatgga 
      tagccggaa acgctggca ctcacttct ggcacgctc 
      acaccggtt tacaaaaaa tggacggtc tttacggca 
      gtgttgtga tccgtcaac caccagcca aggacccca 
      acagtcact tatacgact atgatttga ccacacacg 
      tcatgcttt tgagtgact ggatgcacg aagacgcta 
      ctgaaagat tccccggaa gattggctg tcaacactg 
      gacaagatc ctgagagtt tgctcatca acggaaaag 
      gacaattca gagatccca atactgggt ttatgacca 
      atactccac ttgaagtct ttaccatta cgcctggac 
      gaagataca gatttagga tgattaatt cgttcgctt 
      cagtgtgtc cagctcagt tgacgattc aaggacatg 
      atttgacgc ttattgcta ccgatggag agccagtgc 
      atcctgtga gggttaaca caatcatct cgttttcag 
      gtgaacgat acgattttg taatcaacg ctgaccaaa 
      cacccggtg cttattgga tccaacttc gaggcttag 
      gggagtgtg gcataagaa gagtacaac agttgggta 
      ttctgcggt atgccaaag ggccatacc aacccagcc 
      aagccccgc ccacttatg actacggta ttcctcaag 
      gagtggttt taaatcctt tggacgcaa gatgtaatg 
      aaattcgac cagacgcca tctgtgtaa gccagttaa 
      agaatgccc tcagcattg ataagggta ttctgcgcg 
      aaaaaccag acgttaaaa ttttcttac ccttccgtt 
      tccatatct acacgcctg aggatcttt tcgccccca 
      atacataca acagacatt tagttgcac caaatggcg 
      accacgtca taagtttaa tcgacgaaa tttcctaca 
      tggccccac ctgcacctc ttatatctc aatatgacg 
      atatcgacc cccaacaat tctgcaacg gtgataacc 
      gaccagccg attgccaac aaaactgca tgtgcaccc 
      acaaagttg acattccac tcaacgcca tcgtcgaaa 
      tcgtactag ttgacgaag tacaacaac caaatctaa 
      gccatccat tccacttgc acggctacg ccttcaacg 
      tgatcggca ttgggcgat ctcctgacc aaaacgtga 
      agaaaatca acttgaagc atgcgctag atctggaca 
      gacaagggc ttctccacc gtcaattca accttccgc 
      cagctaaag acacgatag ctgtgccaa acaacggat 
      acgtggtgt tacgactta gggcaaaca atcccggat 
      tctggctgt tccattgtc acttcctct tccacattg 
      tgataggaa tgaatctag ttttgcaag ttggcaccc 
      atgccgatc tgccgccgg ttcctccca atttcccca 
      catgtggag accacgtgc cagaaatta actcgaatc 
      ctaacctcg tttaagatt tagatcgtt cctctgaaa 
      cttcgtttc gtggaacga aaccaatta agttacctt 
      caccaaatt cgtttgttt tgctgtgaa aattgatta 
      ttttcgatc ttgccattc caaaaatct tcaatctag 
      tgttactca atttgtaat ttatattat ttatctaca 
      aatggtttt tattcgcca taattaatt taggtaagt 
      ataagtagt cttcaaccc cttccgata cttccttag 
      aaattacca taggtgctg tatatattg gccgaatgg 
      cgtctgtag actgtagtg taaatacaa tatggcgct 
      tgcattgca aattgttac caatctacc gaatttgca 
      attcgaagc gagtctacc tacttataa taatcagta 
      tttgatgct agagttctt tattttttt ataaaattt 
      ccagaaatt taggtaata atctaaccc agaattcac 
      attgtacag atttcgtct aatggtgtc acactaacc 
      ccagttatt taagattgt cctagtctt ccgcagtct 
      tcaataact atacagagt tactgttat ttttataaa 
      atatgtatt ataaaaaag ttcaataaa aaatgttaa 
      tcaat