Please wait ...
Gene information
General information
TC010479 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451027.1: 111948..130953
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.

    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.

    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01699

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    RNAi sequence
    Left: Right:
    Protein sequence
    • >TC010479 RA
    CDS sequence
    • >TC010479 RA
      atgccgtct gaaaagtcc gtttacctg cccaaccct 
      tccgtggcg aaaaatgcg ttcgtctcg tccttcgca 
      caataccag gaaatgtac cagaaatcg atagacaac 
      cctgaggag ttctggggt gaaatcgcc aagcaattc 
      tactgggag accccgatc gacattaac tcgtttttt 
      cgctacaat tttgacatc agaaagggg cccatttac 
      accaaatgg ctggacggg gcgaccacg aatatcgcc 
      tataatttg ctggatcga aatgtgaaa aatgggttc 
      ggggacaaa gttgcgttt tattgggag gggaaccac 
      ccggacgat tacagccgc ctaacgtat aaaaaatta 
      ctggaggaa gtttgcaga tttgcgaat gttttgcga 
      agtaaagga gtgaaaaaa ggcgacagg gttgctgtt 
      tacatgcct atgatcctt gaagctgtg ataacctta 
      ctaggatgt gcacggatt ggagcagtt cattccctt 
      gtgtttgca ggattttcg gccgattct ttcgccgaa 
      cgcatcctc gactgtgaa gcccgcgtt ctcgtaaca 
      gcagacggc gtctggcga ggagaaaaa ctcctctcc 
      ttgaaaaat atctgtgat caatcgatg aataaatgt 
      gcacagaaa ggccacaat gtgaaaact tgcatagtg 
      gtgtcgcac ttgggccgc gttacttcg ccatctggg 
      gttcaaaag aatcggaac aataaaacg cctatgcaa 
      gaaggtcgc gatgtttgg tggcacgat attgttcct 
      caagcgtca actgattgt gtgccagag tgggttcaa 
      gctgaagat cccttgttt atgttgtac acgagtggt 
      tcaactggg aagcccaaa ggtgtggta cacacaaca 
      gcggggtat ttgttgtac gctgccacc acgtttaaa 
      tacgtgttt gattacaag cccaatgat gtttattgg 
      tgtactgct gatattggc tggatcact ggacacacg 
      tatgtggtg tatgggccg ttggcgaat tgtgcaact 
      tcggttctg tatgaagga actccattt tatccaaat 
      aatgacaga ttctggcta gtcgttaaa aagtataag 
      gttaatcag ttttacact gcccctact gccattaga 
      gctttaatg aaatttgaa gatgatctt gttaaaaga 
      gtcgaccta tcctcttta aaagtatta ggctcagtt 
      ggtgagcca ataaaccct gaagcttgg atgtggtat 
      tacaaacta gtcggaaga gaaaaatgt tcaattgtt 
      gacaccttc tggcagact gaaacaggg ggtcatgtt 
      ttgacaccg cttcctggg gcaactcct atgaaacca 
      ggcgctgct gcattcccc ttctttggc gtccaacca 
      gccctcttg gacgagtcc ggcaacgag ataaagggc 
      gaaggtgaa ggttacctc gttttcgct cgcccttgg 
      cccggcata atgcgcacc ctttacaac aaccacccc 
      cgatacgaa accacctac ttctccaaa ttccctggc 
      tattactgc actggagac ggtgctcga agagacgcc 
      gacggctat ctctgggtc acaggacgt gttgatgac 
      atgttgaac gtctccgga catttaatg tcaaccgct 
      gaagtcgaa tcggttcta accgaacac ccgaatgtt 
      gccgaggct gcagttgtt gcaaaacca cacccagtc 
      aaaggcgaa tgcctttat tgtttcatc acaacgacg 
      gacacgact cgctttgac gacaagttg ttgactgag 
      ttgaagcag cttgtcaga gagaggata ggacctttt 
      gcacagcct gatgttgtg caaaatgcg ccagctttg 
      cctaagaca aggtccggg aaaataatg cggaggatt 
      ttgcggaag gtggctgtt aatgaccga gatgtgggg 
      gatgtgagt acgttagct gatagtcat gttgttgag 
      gagttgttc gcgagtcga ccagtgaac gcttag
    mRNA sequence
    • >TC010479 RA
      taaacgaat aatcagtgg ctggcgcgt ccgccactt 
      cagtcgcgt tcaagtttt gttttcgaa aggtggtga 
      aaaaaccgc gtcaaaatg ccgtctgaa aagtccgtt 
      tacctgccc aacccttcc gtggcgaaa aatgcgttc 
      gtctcgtcc ttcgcacaa taccaggaa atgtaccag 
      aaatcgata gacaaccct gaggagttc tggggtgaa 
      atcgccaag caattctac tgggagacc ccgatcgac 
      attaactcg ttttttcgc tacaatttt gacatcaga 
      aaggggccc atttacacc aaatggctg gacggggcg 
      accacgaat atcgcctat aatttgctg gatcgaaat 
      gtgaaaaat gggttcggg gacaaagtt gcgttttat 
      tgggagggg aaccacccg gacgattac agccgccta 
      acgtataaa aaattactg gaggaagtt tgcagattt 
      gcgaatgtt ttgcgaagt aaaggagtg aaaaaaggc 
      gacagggtt gctgtttac atgcctatg atccttgaa 
      gctgtgata accttacta ggatgtgca cggattgga 
      gcagttcat tcccttgtg tttgcagga ttttcggcc 
      gattctttc gccgaacgc atcctcgac tgtgaagcc 
      cgcgttctc gtaacagca gacggcgtc tggcgagga 
      gaaaaactc ctctccttg aaaaatatc tgtgatcaa 
      tcgatgaat aaatgtgca cagaaaggc cacaatgtg 
      aaaacttgc atagtggtg tcgcacttg ggccgcgtt 
      acttcgcca tctggggtt caaaagaat cggaacaat 
      aaaacgcct atgcaagaa ggtcgcgat gtttggtgg 
      cacgatatt gttcctcaa gcgtcaact gattgtgtg 
      ccagagtgg gttcaagct gaagatccc ttgtttatg 
      ttgtacacg agtggttca actgggaag cccaaaggt 
      gtggtacac acaacagcg gggtatttg ttgtacgct 
      gccaccacg tttaaatac gtgtttgat tacaagccc 
      aatgatgtt tattggtgt actgctgat attggctgg 
      atcactgga cacacgtat gtggtgtat gggccgttg 
      gcgaattgt gcaacttcg gttctgtat gaaggaact 
      ccattttat ccaaataat gacagattc tggctagtc 
      gttaaaaag tataaggtt aatcagttt tacactgcc 
      cctactgcc attagagct ttaatgaaa tttgaagat 
      gatcttgtt aaaagagtc gacctatcc tctttaaaa 
      gtattaggc tcagttggt gagccaata aaccctgaa 
      gcttggatg tggtattac aaactagtc ggaagagaa 
      aaatgttca attgttgac accttctgg cagactgaa 
      acagggggt catgttttg acaccgctt cctggggca 
      actcctatg aaaccaggc gctgctgca ttccccttc 
      tttggcgtc caaccagcc ctcttggac gagtccggc 
      aacgagata aagggcgaa ggtgaaggt tacctcgtt 
      ttcgctcgc ccttggccc ggcataatg cgcaccctt 
      tacaacaac cacccccga tacgaaacc acctacttc 
      tccaaattc cctggctat tactgcact ggagacggt 
      gctcgaaga gacgccgac ggctatctc tgggtcaca 
      ggacgtgtt gatgacatg ttgaacgtc tccggacat 
      ttaatgtca accgctgaa gtcgaatcg gttctaacc 
      gaacacccg aatgttgcc gaggctgca gttgttgca 
      aaaccacac ccagtcaaa ggcgaatgc ctttattgt 
      ttcatcaca acgacggac acgactcgc tttgacgac 
      aagttgttg actgagttg aagcagctt gtcagagag 
      aggatagga ccttttgca cagcctgat gttgtgcaa 
      aatgcgcca gctttgcct aagacaagg tccgggaaa 
      ataatgcgg aggattttg cggaaggtg gctgttaat 
      gaccgagat gtgggggat gtgagtacg ttagctgat 
      agtcatgtt gttgaggag ttgttcgcg agtcgacca 
      gtgaacgct tagggttgt tgttgatct gttgaaatn 
      gtattattg tagttttta ttttgagag ttttttaca 
      gcacgcaag ctaacatgt ctctgaaaa gagttatag 
      aaataaaag gctgatttt atgtaggtt aacgtagag 
      cagcacttc acattaact aactaaata ggtaagtat 
      ttcttgcac aaaaatttt tgttggttg tgtttttac 
      ttactgtta cactgaaag tgtctacgc atacgagtc 
      cttgctaac ctacatatc atgatcaga gagtgtata 
      catatgttt tgataaaat atacttatt ctagattca 
      ggtgcaatc actattatt tttttattt atacgttgt 
      aaatgtata gttgaagga aaatattca aatgtacgc 
      tttatgaaa atatgtaga aatttaatt attcaagca 
      atttattgt gcggagttt agtaatatt ggcatgatt 
      gttataaat caatttatg tacacatct aattgcttg 
      taatgtatt tatgtaagt atatgttgt actgaataa 
      agaggaaag cagtga

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY