Status
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Gene information
General information
TC033105
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010469,TC010468
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 6817506..6855645
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033105
    iBeetle screen iB_01697
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    legs segment slightly shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033105 RA
      MVAGQNVDT QQNADMYCA LYGEYYPDN QYFIQGAPP 
      EMCPAHVCA MQSEFGPVT VPMVSTNSS PPIAMPVQV 
      PPGHVVQQI VDESGTLRH VILSPQHPA GLVPLTSHN 
      PQHYGAGPG NSTNQPQQA FYQGLPTGF PASHFHSNI 
      PPGHPGPSP TRLGHSPPL NQIYYKDER AKRQYIKLK 
      KKHQDKQKL DSMQKDLVN GLRRPSAKD KDMNSVGTS 
      EDGEESSIP DEEDSVQII ADILSSVQA PKVSELSSR 
      SALLLWAPP TKLSEASST DGPEIDVGD LRYEVLLSD 
      KSKEMKFKS IYSGTSLSC RIRDLKPGQ EYSVCLQVH 
      YDELQGAAT DPVKFTTPP CEPDQPQPP KLHQRQKTS 
      LQLKWNSVN DNGSHITCY ILEYDEGKG GDFVEFHRG 
      RVKQYTIHK LQPATPYRF RLAAMNEIG KSGYSEVVT 
      YNTCNNPPT KPPPPVLAE ASINYLHLQ WSKRPSDEE 
      FTLQMNDSR TSYRPVYNG RETICICTG LSNFTEYKF 
      RLRAENDGG QSPWSDEVT YQTLPDRPR EPSKPVVKG 
      RIHAHSFKL KWEPPNYTG GADITEYIL EVNSGSGYE 
      VVYTGTETE AVCDKLTPG TTYQLRASC ISAGGRSNY 
      SNPCTVTTD AIAPGQCAA PRLHGKPKS SSITIRWCE 
      PDYNGGAPV LEYEVEMAG PPDHTRNLV HKSKETECT 
      AANLTPGCE YEFFVRAVN RIGPGLWSD PLKVTSGAA 
      PPDVIETLA TNCKSPFHI FVEWREPPS NGAPILDYR 
      LEISTSNND FHLVYQGAE NYHDVKGLS PFTTYYFRV 
      QACNSAGCS LYSPVAATV TPAAPPSSV TTLRSDSTP 
      TSITLNWNE PSNNGSEIT HYNIEFGDK LIVTDGPVR 
      EYQIDNLQP ETTYKIKIQ AVNAIGTGS LSSTLKVST 
      LKLPPAAPR LECIGIGHN YLKLKWGDG KNVDYTHYL 
      IEMENGRTH DFQCVYKGT ALTYKVNKL HELTTYRFR 
      ICASNDAGV GDFSDIYEF TTSIAPPAV VKQPKLIEV 
      DQTSCVIEW MPSKNQFVD PVIFQVQVA RVKDQFFKE 
      TYRGAESKC SIENLESGV EYSARVCPI RLTASGELV 
      GPSSPLLHF STLIAEPTV VSKISTNPS SPTHSHRNH 
      RTALTSILH RLKTLLNTP LVVAIVVTI IGIIISIWI 
      MSFL
    • >TC033105 RB
      MVKVTDGPM AEAKPATTA TTTQSPTIS NNNATNSNS 
      TTHTHIRKP RNHNHTHHI HQTLHHYTY LHHHCTHPS 
      DNAETNGSN GAGSPAKFH FGPGFEPQI SGGNYCPGP 
      SQSQNNSEY VVLFHVNPG VTIRFQMGD SFEVLQGPV 
      TVPMVSTNS SPPIAMPVQ VPPGHVVQQ IVDESGTLR 
      HVILSPQHP AGLVPLTSH NPQHYGAGP GNSTNQPQQ 
      AFYQGLPTG FPASHFHSN IPPGHPGPS PTRLGHSPP 
      LNQIYYKDE RAKRQYIKL KKKHQDKQK LDSMQKDLV 
      NGLRRPSAK DKDMNSVGT SEDGEESSI PDEEDSVQI 
      IADILSSVQ APKVSELSS RSALLLWAP PTKLSEASS 
      TDGPEIDVG DLRYEVLLS DKSKEMKFK SIYSGTSLS 
      CRIRDLKPG QEYSVCLQV HYDELQGAA TDPVKFTTP 
      PCEPDQPQP PKLHQRQKT SLQLKWNSV NDNGSHITC 
      YILEYDEGK GGDFVEFHR GRVKQYTIH KLQPATPYR 
      FRLAAMNEI GKSGYSEVV TYNTCNNPP TKPPPPVLA 
      EASINYLHL QWSKRPSDE EFTLQMNDS RTSYRPVYN 
      GRETICICT GLSNFTEYK FRLRAENDG GQSPWSDEV 
      TYQTLPDRP REPSKPVVK GRIHAHSFK LKWEPPNYT 
      GGADITEYI LEVNSGSGY EVVYTGTET EAVCDKLTP 
      GTTYQLRAS CISAGGRSN YSNPCTVTT DAIAPGQCA 
      APRLHGKPK SSSITIRWC EPDYNGGAP VLEYEVEMA 
      GPPDHTRNL VHKSKETEC TAANLTPGC EYEFFVRAV 
      NRIGPGLWS DPLKVTSGA APPDVIETL ATNCKSPFH 
      IFVEWREPP SNGAPILDY RLEISTSNN DFHLVYQGA 
      ENYHDVKGL SPFTTYYFR VQACNSAGC SLYSPVAAT 
      VTPAAPPSS VTTLRSDST PTSITLNWN EPSNNGSEI 
      THYNIEFGD KLIVTDGPV REYQIDNLQ PETTYKIKI 
      QAVNAIGTG SLSSTLKVS TLKLPPAAP RLECIGIGH 
      NYLKLKWGD GKNVDYTHY LIEMENGRT HDFQCVYKG 
      TALTYKVNK LHELTTYRF RICASNDAG VGDFSDIYE 
      FTTSIAPPA VVKQPKLIE VDQTSCVIE WMPSKNQFV 
      DPVIFQVQV ARVKDQFFK ETYRGAESK CSIENLESG 
      VEYSARVCP IRLTASGEL VGPSSPLLH FSTLIAEPT 
      VVSKISTNP SSPTHSHRN HRTALTSIL HRLKTLLNT 
      PLVVAIVVT IIGIIISIW IMSFL
    CDS sequence
    • >TC033105 RA
      atggtggcc ggccaaaac gtggacacc caacagaat 
      gccgacatg tattgtgcc ctttatgga gaatactac 
      cctgacaat cagtacttc attcaaggg gcgcccccc 
      gagatgtgc cccgctcac gtctgcgcc atgcaaagc 
      gaattcggt ccagtaaca gttccgatg gtatcaacg 
      aattcgtcg ccacccatt gcgatgcca gtacaagtt 
      cctccaggt catgtcgta caacaaatt gttgacgaa 
      agcggcacc ttgcgccat gtgatactc tccccacaa 
      catccagcc ggactcgtg ccattaacc tcacataat 
      ccacagcac tatggtgcc ggtccaggg aatagcaca 
      aaccagcct caacaagca ttctatcaa ggcctgccg 
      acggggttt ccagcctca catttccac tcgaacatc 
      ccgccaggt catccagga ccaagtcct actcgtctc 
      ggacattcc ccaccttta aatcaaatt tattacaag 
      gatgaacga gccaaacga caatatatt aagttgaaa 
      aaaaaacat caagacaaa cagaaatta gacagtatg 
      cagaaggac ctggtgaat ggattgagg aggccgtca 
      gccaaagat aaagacatg aacagtgtt ggaacgtca 
      gaggatggg gaggagagc agtattccg gacgaagag 
      gattccgtt caaatcatt gctgatatt ttgtcgtcc 
      gtccaggct ccaaaggta tctgaactg agttcccga 
      agtgcctta ttgctgtgg gcgccgccc acgaaactc 
      tcggaagct tcgagtacg gacggcccc gagatcgac 
      gtcggtgat ttacgctac gaagtatta cttagtgat 
      aagagcaaa gaaatgaaa tttaagtca atatacagc 
      ggtacttcg ctgtcttgt cgcatccgg gacctaaaa 
      ccgggccag gagtactcc gtctgtctc caggtgcac 
      tacgacgag ctccagggc gccgccacc gaccccgtc 
      aagttcacc acgccgccg tgcgagccc gaccagccg 
      cagcccccc aagctgcac cagcggcag aaaacctcg 
      ctgcagctg aagtggaac agcgtcaac gacaacggc 
      tcgcacatc acgtgctac atcctggag tacgacgag 
      ggcaaaggc ggcgacttc gtggagttc caccgcggg 
      agagtgaaa cagtacacc atacacaag ttgcaacca 
      gcgaccccg taccggttc aggctggcg gcgatgaac 
      gagataggg aagagcggg tactcggaa gtggtcact 
      tacaacact tgtaacaac ccgccgacg aaaccgccg 
      cctccggtg ctggcagag gcctcgatc aattacctc 
      cacttgcag tggagcaaa aggcctagc gatgaggag 
      tttacgctt cagatgaac gactcgagg actagctat 
      cggccggtt tacaacgga agggagacg atttgtatt 
      tgtacgggt ctgagtaat tttacggag tataagttc 
      cggctgagg gcggagaat gacggggga cagtcgccg 
      tggtccgac gaggtgacg tatcagacc ttgcctgat 
      cgccccagg gagccctcc aagcccgtc gtcaagggc 
      cggatccac gcacactcg ttcaagctg aagtgggag 
      ccgccgaac tacaccggc ggcgccgac atcaccgag 
      tatatcctc gaggtgaac tccggcagc ggctacgag 
      gtcgtctac accggaacg gaaacggaa gcagtctgt 
      gataaacta acccccggc accacctac cagttgcgc 
      gcgagctgc atcagtgcc ggcggccgt agcaactac 
      tcaaaccct tgcactgtg acgacggac gccatcgcc 
      cccggccag tgcgccgcc ccccgtctg cacggcaag 
      cccaagtcg agcagcatc accatcagg tggtgcgag 
      cccgactac aacggcggg gcgccggtg ctggagtac 
      gaggtggag atggcgggc ccgcccgac cacacgcga 
      aatctagtt cacaaaagc aaagagacc gaatgcacg 
      gctgcgaat ctcactccg ggctgtgag tacgagttc 
      tttgtgaga gccgtcaac aggataggg cccggactg 
      tggtcggat cctttgaaa gttaccagc ggagcagct 
      ccgccagat gtcattgag acgctcgcg accaattgc 
      aaatcacca ttccatata ttcgtcgaa tggcgagag 
      ccgccgtct aacggagcg cccattcta gactataga 
      ttagaaatt agcacaagt aataatgac ttccattta 
      gtttatcaa ggagccgaa aattaccat gacgtcaag 
      ggactcagc cccttcacg acgtactac ttccgggtg 
      caagcatgc aactctgcc gggtgcagt ctgtactcc 
      cccgtggcg gcgaccgtc acccccgca gcgccccct 
      tccagcgta acgaccctc cgcagcgac tccaccccc 
      acgtcaata acgctcaac tggaacgag ccctccaat 
      aacggttcg gaaatcacc cactacaat atcgaattc 
      ggtgataag ctcatcgtt actgacggt cccgtgaga 
      gagtatcaa atcgacaat ctccaacca gagacaacc 
      tacaaaatc aaaatccaa gccgtaaat gcaatcggc 
      actggctct ctttcctcc acgcttaaa gtatcgaca 
      ctgaaactg ccgccagca gctcctaga cttgaatgc 
      atcggcatt ggacacaac tatctcaaa ctgaaatgg 
      ggcgacggc aaaaacgtc gactacact cactatctg 
      atcgaaatg gaaaacgga cgcacgcac gatttccaa 
      tgcgtctat aaaggaaca gctctgaca tacaaagtt 
      aataaattg catgaacta acaacatat aggtttagg 
      atctgcgca tcgaacgat gctggtgta ggggatttt 
      tccgatatt tatgaattt acgaccagt atagcaccg 
      cctgcagtc gtcaaacaa cccaaattg atcgaagtc 
      gatcaaacg agctgcgtg atcgaatgg atgccctcc 
      aaaaatcag ttcgtggat ccggtcatc ttccaagtt 
      caggtggcc agagtcaag gaccagttt ttcaaagag 
      acatacaga ggcgctgag tcgaagtgt tctatcgag 
      aatttagaa tcaggtgtg gaatacagc gctcgtgta 
      tgtccgatt agactgacg gccagcggc gagctggtg 
      ggcccctct tcgcctctc ttgcacttt tcgacactg 
      attgccgaa ccgactgta gtatcaaaa atctcgaca 
      aatcccagc tcaccgact cactcccac cgaaaccac 
      cggactgca ctgacgtca attctgcac cgcttaaag 
      acactccta aatacgccc cttgtcgtt gccatcgtg 
      gtaacaatc ataggaatt atcatttcg atatggatt 
      atgtcgttt ctctaa
    • >TC033105 RB
      atggtgaag gtaacagac gggccgatg gcagaggct 
      aagcctgca acaacagca acaacaaca caatctccg 
      acaattagt aacaacaac gcaactaac agcaacagt 
      acaacacac actcatatt cgtaaaccg cgtaaccac 
      aatcatacc catcatatt caccagaca ttacaccat 
      tacacctac ttacaccac cactgtacc catccaagt 
      gacaatgcg gaaaccaac ggctccaat ggagctgga 
      tctccggca aaattccat ttcgggccc ggattcgag 
      ccacaaatt tccgggggc aattactgc ccagggccg 
      agccagagt cagaacaac tccgaatac gtcgtgctg 
      ttccacgtc aatcctggc gtcaccatc aggttccag 
      atgggggat agcttcgag gtcctgcaa ggtccagta 
      acagttccg atggtatca acgaattcg tcgccaccc 
      attgcgatg ccagtacaa gttcctcca ggtcatgtc 
      gtacaacaa attgttgac gaaagcggc accttgcgc 
      catgtgata ctctcccca caacatcca gccggactc 
      gtgccatta acctcacat aatccacag cactatggt 
      gccggtcca gggaatagc acaaaccag cctcaacaa 
      gcattctat caaggcctg ccgacgggg tttccagcc 
      tcacatttc cactcgaac atcccgcca ggtcatcca 
      ggaccaagt cctactcgt ctcggacat tccccacct 
      ttaaatcaa atttattac aaggatgaa cgagccaaa 
      cgacaatat attaagttg aaaaaaaaa catcaagac 
      aaacagaaa ttagacagt atgcagaag gacctggtg 
      aatggattg aggaggccg tcagccaaa gataaagac 
      atgaacagt gttggaacg tcagaggat ggggaggag 
      agcagtatt ccggacgaa gaggattcc gttcaaatc 
      attgctgat attttgtcg tccgtccag gctccaaag 
      gtatctgaa ctgagttcc cgaagtgcc ttattgctg 
      tgggcgccg cccacgaaa ctctcggaa gcttcgagt 
      acggacggc cccgagatc gacgtcggt gatttacgc 
      tacgaagta ttacttagt gataagagc aaagaaatg 
      aaatttaag tcaatatac agcggtact tcgctgtct 
      tgtcgcatc cgggaccta aaaccgggc caggagtac 
      tccgtctgt ctccaggtg cactacgac gagctccag 
      ggcgccgcc accgacccc gtcaagttc accacgccg 
      ccgtgcgag cccgaccag ccgcagccc cccaagctg 
      caccagcgg cagaaaacc tcgctgcag ctgaagtgg 
      aacagcgtc aacgacaac ggctcgcac atcacgtgc 
      tacatcctg gagtacgac gagggcaaa ggcggcgac 
      ttcgtggag ttccaccgc gggagagtg aaacagtac 
      accatacac aagttgcaa ccagcgacc ccgtaccgg 
      ttcaggctg gcggcgatg aacgagata gggaagagc 
      gggtactcg gaagtggtc acttacaac acttgtaac 
      aacccgccg acgaaaccg ccgcctccg gtgctggca 
      gaggcctcg atcaattac ctccacttg cagtggagc 
      aaaaggcct agcgatgag gagtttacg cttcagatg 
      aacgactcg aggactagc tatcggccg gtttacaac 
      ggaagggag acgatttgt atttgtacg ggtctgagt 
      aattttacg gagtataag ttccggctg agggcggag 
      aatgacggg ggacagtcg ccgtggtcc gacgaggtg 
      acgtatcag accttgcct gatcgcccc agggagccc 
      tccaagccc gtcgtcaag ggccggatc cacgcacac 
      tcgttcaag ctgaagtgg gagccgccg aactacacc 
      ggcggcgcc gacatcacc gagtatatc ctcgaggtg 
      aactccggc agcggctac gaggtcgtc tacaccgga 
      acggaaacg gaagcagtc tgtgataaa ctaaccccc 
      ggcaccacc taccagttg cgcgcgagc tgcatcagt 
      gccggcggc cgtagcaac tactcaaac ccttgcact 
      gtgacgacg gacgccatc gcccccggc cagtgcgcc 
      gccccccgt ctgcacggc aagcccaag tcgagcagc 
      atcaccatc aggtggtgc gagcccgac tacaacggc 
      ggggcgccg gtgctggag tacgaggtg gagatggcg 
      ggcccgccc gaccacacg cgaaatcta gttcacaaa 
      agcaaagag accgaatgc acggctgcg aatctcact 
      ccgggctgt gagtacgag ttctttgtg agagccgtc 
      aacaggata gggcccgga ctgtggtcg gatcctttg 
      aaagttacc agcggagca gctccgcca gatgtcatt 
      gagacgctc gcgaccaat tgcaaatca ccattccat 
      atattcgtc gaatggcga gagccgccg tctaacgga 
      gcgcccatt ctagactat agattagaa attagcaca 
      agtaataat gacttccat ttagtttat caaggagcc 
      gaaaattac catgacgtc aagggactc agccccttc 
      acgacgtac tacttccgg gtgcaagca tgcaactct 
      gccgggtgc agtctgtac tcccccgtg gcggcgacc 
      gtcaccccc gcagcgccc ccttccagc gtaacgacc 
      ctccgcagc gactccacc cccacgtca ataacgctc 
      aactggaac gagccctcc aataacggt tcggaaatc 
      acccactac aatatcgaa ttcggtgat aagctcatc 
      gttactgac ggtcccgtg agagagtat caaatcgac 
      aatctccaa ccagagaca acctacaaa atcaaaatc 
      caagccgta aatgcaatc ggcactggc tctctttcc 
      tccacgctt aaagtatcg acactgaaa ctgccgcca 
      gcagctcct agacttgaa tgcatcggc attggacac 
      aactatctc aaactgaaa tggggcgac ggcaaaaac 
      gtcgactac actcactat ctgatcgaa atggaaaac 
      ggacgcacg cacgatttc caatgcgtc tataaagga 
      acagctctg acatacaaa gttaataaa ttgcatgaa 
      ctaacaaca tataggttt aggatctgc gcatcgaac 
      gatgctggt gtaggggat ttttccgat atttatgaa 
      tttacgacc agtatagca ccgcctgca gtcgtcaaa 
      caacccaaa ttgatcgaa gtcgatcaa acgagctgc 
      gtgatcgaa tggatgccc tccaaaaat cagttcgtg 
      gatccggtc atcttccaa gttcaggtg gccagagtc 
      aaggaccag tttttcaaa gagacatac agaggcgct 
      gagtcgaag tgttctatc gagaattta gaatcaggt 
      gtggaatac agcgctcgt gtatgtccg attagactg 
      acggccagc ggcgagctg gtgggcccc tcttcgcct 
      ctcttgcac ttttcgaca ctgattgcc gaaccgact 
      gtagtatca aaaatctcg acaaatccc agctcaccg 
      actcactcc caccgaaac caccggact gcactgacg 
      tcaattctg caccgctta aagacactc ctaaatacg 
      ccccttgtc gttgccatc gtggtaaca atcatagga 
      attatcatt tcgatatgg attatgtcg tttctctaa
    mRNA sequence
    • >TC033105 RA
      tcagatttt aataggagg tatagtgtt ttgtggaaa 
      gaacgttaa acgcgccgg aacgtgtgt gaccgaaag 
      tatgaaatt tgttcagtg tcctcgcac cccagagaa 
      ctattgacc agcccgtag aggcctagc ggaggtgcc 
      accatccag ccgggagtc ctccgtata ttgtctgtg 
      atctgttat gtaattgtg catgattaa atggtgaca 
      gtgtttggt aaaagtgca aagtgtgtt tcttaacgg 
      taacagtgc tagtcgacg ttcagggat ggtggccgg 
      ccaaaacgt ggacaccca acagaatgc cgacatgta 
      ttgtgccct ttatggaga atactaccc tgacaatca 
      gtacttcat tcaaggggc gccccccga gatgtgccc 
      cgctcacgt ctgcgccat gcaaagcga attcggtcc 
      agtaacagt tccgatggt atcaacgaa ttcgtcgcc 
      acccattgc gatgccagt acaagttcc tccaggtca 
      tgtcgtaca acaaattgt tgacgaaag cggcacctt 
      gcgccatgt gatactctc cccacaaca tccagccgg 
      actcgtgcc attaacctc acataatcc acagcacta 
      tggtgccgg tccagggaa tagcacaaa ccagcctca 
      acaagcatt ctatcaagg cctgccgac ggggtttcc 
      agcctcaca tttccactc gaacatccc gccaggtca 
      tccaggacc aagtcctac tcgtctcgg acattcccc 
      acctttaaa tcaaattta ttacaagga tgaacgagc 
      caaacgaca atatattaa gttgaaaaa aaaacatca 
      agacaaaca gaaattaga cagtatgca gaaggacct 
      ggtgaatgg attgaggag gccgtcagc caaagataa 
      agacatgaa cagtgttgg aacgtcaga ggatgggga 
      ggagagcag tattccgga cgaagagga ttccgttca 
      aatcattgc tgatatttt gtcgtccgt ccaggctcc 
      aaaggtatc tgaactgag ttcccgaag tgccttatt 
      gctgtgggc gccgcccac gaaactctc ggaagcttc 
      gagtacgga cggccccga gatcgacgt cggtgattt 
      acgctacga agtattact tagtgataa gagcaaaga 
      aatgaaatt taagtcaat atacagcgg tacttcgct 
      gtcttgtcg catccggga cctaaaacc gggccagga 
      gtactccgt ctgtctcca ggtgcacta cgacgagct 
      ccagggcgc cgccaccga ccccgtcaa gttcaccac 
      gccgccgtg cgagcccga ccagccgca gccccccaa 
      gctgcacca gcggcagaa aacctcgct gcagctgaa 
      gtggaacag cgtcaacga caacggctc gcacatcac 
      gtgctacat cctggagta cgacgaggg caaaggcgg 
      cgacttcgt ggagttcca ccgcgggag agtgaaaca 
      gtacaccat acacaagtt gcaaccagc gaccccgta 
      ccggttcag gctggcggc gatgaacga gatagggaa 
      gagcgggta ctcggaagt ggtcactta caacacttg 
      taacaaccc gccgacgaa accgccgcc tccggtgct 
      ggcagaggc ctcgatcaa ttacctcca cttgcagtg 
      gagcaaaag gcctagcga tgaggagtt tacgcttca 
      gatgaacga ctcgaggac tagctatcg gccggttta 
      caacggaag ggagacgat ttgtatttg tacgggtct 
      gagtaattt tacggagta taagttccg gctgagggc 
      ggagaatga cgggggaca gtcgccgtg gtccgacga 
      ggtgacgta tcagacctt gcctgatcg ccccaggga 
      gccctccaa gcccgtcgt caagggccg gatccacgc 
      acactcgtt caagctgaa gtgggagcc gccgaacta 
      caccggcgg cgccgacat caccgagta tatcctcga 
      ggtgaactc cggcagcgg ctacgaggt cgtctacac 
      cggaacgga aacggaagc agtctgtga taaactaac 
      ccccggcac cacctacca gttgcgcgc gagctgcat 
      cagtgccgg cggccgtag caactactc aaacccttg 
      cactgtgac gacggacgc catcgcccc cggccagtg 
      cgccgcccc ccgtctgca cggcaagcc caagtcgag 
      cagcatcac catcaggtg gtgcgagcc cgactacaa 
      cggcggggc gccggtgct ggagtacga ggtggagat 
      ggcgggccc gcccgacca cacgcgaaa tctagttca 
      caaaagcaa agagaccga atgcacggc tgcgaatct 
      cactccggg ctgtgagta cgagttctt tgtgagagc 
      cgtcaacag gatagggcc cggactgtg gtcggatcc 
      tttgaaagt taccagcgg agcagctcc gccagatgt 
      cattgagac gctcgcgac caattgcaa atcaccatt 
      ccatatatt cgtcgaatg gcgagagcc gccgtctaa 
      cggagcgcc cattctaga ctatagatt agaaattag 
      cacaagtaa taatgactt ccatttagt ttatcaagg 
      agccgaaaa ttaccatga cgtcaaggg actcagccc 
      cttcacgac gtactactt ccgggtgca agcatgcaa 
      ctctgccgg gtgcagtct gtactcccc cgtggcggc 
      gaccgtcac ccccgcagc gcccccttc cagcgtaac 
      gaccctccg cagcgactc cacccccac gtcaataac 
      gctcaactg gaacgagcc ctccaataa cggttcgga 
      aatcaccca ctacaatat cgaattcgg tgataagct 
      catcgttac tgacggtcc cgtgagaga gtatcaaat 
      cgacaatct ccaaccaga gacaaccta caaaatcaa 
      aatccaagc cgtaaatgc aatcggcac tggctctct 
      ttcctccac gcttaaagt atcgacact gaaactgcc 
      gccagcagc tcctagact tgaatgcat cggcattgg 
      acacaacta tctcaaact gaaatgggg cgacggcaa 
      aaacgtcga ctacactca ctatctgat cgaaatgga 
      aaacggacg cacgcacga tttccaatg cgtctataa 
      aggaacagc tctgacata caaagttaa taaattgca 
      tgaactaac aacatatag gtttaggat ctgcgcatc 
      gaacgatgc tggtgtagg ggatttttc cgatattta 
      tgaatttac gaccagtat agcaccgcc tgcagtcgt 
      caaacaacc caaattgat cgaagtcga tcaaacgag 
      ctgcgtgat cgaatggat gccctccaa aaatcagtt 
      cgtggatcc ggtcatctt ccaagttca ggtggccag 
      agtcaagga ccagttttt caaagagac atacagagg 
      cgctgagtc gaagtgttc tatcgagaa tttagaatc 
      aggtgtgga atacagcgc tcgtgtatg tccgattag 
      actgacggc cagcggcga gctggtggg cccctcttc 
      gcctctctt gcacttttc gacactgat tgccgaacc 
      gactgtagt atcaaaaat ctcgacaaa tcccagctc 
      accgactca ctcccaccg aaaccaccg gactgcact 
      gacgtcaat tctgcaccg cttaaagac actcctaaa 
      tacgcccct tgtcgttgc catcgtggt aacaatcat 
      aggaattat catttcgat atggattat gtcgtttct 
      ctaagtgca attgatgtg tggtcgctt tgctcactc 
      caaacaaac aaacttaaa ctaagcgat actaccctg 
      ccgtttggc gacgaaaag tggaatata aagtattat 
      taaacaggg cttattata attagagga ccaaagcgc 
      aagttttgg tatttttat acgtgtttc cgtacttgg 
      cgtggtgct atccaatta attaacgat gtgatgtct 
      aacggcggt caccgccgc agatgacct gtagtttga 
      tatatgtgt tgttattcc agtcatgtg tatagtaaa 
      gcaaacccg tacacatat tgtatgtat ttatgaagc 
      aggattaac taaatgaat tttcaagtg gctggaaag 
      tctgtacct ataaaattt taaatatat atacttata 
      taatacagc atataggtt aatgaaatt atataactc 
      taatgttaa tctattaag tatattaca atctaatct 
      tttattgtt taaagttgg ctaaaattg ggtttgtat 
      atgtgctac ttcctgcac agtcgacag agatatatt 
      aatatatta taggtataa aa
    • >TC033105 RB
      acgtaaaca aaaatcaat aaacatggt ggtaaatgt 
      gtaagtgtc ttcggggtt tctaatatg tttttataa 
      aaaacgttt tcccaacat aaggctatt taaaaaaac 
      gaatggtga aggtaacag acgggccga tggcagagg 
      ctaagcctg caacaacag caacaacaa cacaatctc 
      cgacaatta gtaacaaca acgcaacta acagcaaca 
      gtacaacac acactcata ttcgtaaac cgcgtaacc 
      acaatcata cccatcata ttcaccaga cattacacc 
      attacacct acttacacc accactgta cccatccaa 
      gtgacaatg cggaaacca acggctcca atggagctg 
      gatctccgg caaaattcc atttcgggc ccggattcg 
      agccacaaa tttccgggg gcaattact gcccagggc 
      cgagccaga gtcagaaca actccgaat acgtcgtgc 
      tgttccacg tcaatcctg gcgtcacca tcaggttcc 
      agatggggg atagcttcg aggtcctgc aaggtccag 
      taacagttc cgatggtat caacgaatt cgtcgccac 
      ccattgcga tgccagtac aagttcctc caggtcatg 
      tcgtacaac aaattgttg acgaaagcg gcaccttgc 
      gccatgtga tactctccc cacaacatc cagccggac 
      tcgtgccat taacctcac ataatccac agcactatg 
      gtgccggtc cagggaata gcacaaacc agcctcaac 
      aagcattct atcaaggcc tgccgacgg ggtttccag 
      cctcacatt tccactcga acatcccgc caggtcatc 
      caggaccaa gtcctactc gtctcggac attccccac 
      ctttaaatc aaatttatt acaaggatg aacgagcca 
      aacgacaat atattaagt tgaaaaaaa aacatcaag 
      acaaacaga aattagaca gtatgcaga aggacctgg 
      tgaatggat tgaggaggc cgtcagcca aagataaag 
      acatgaaca gtgttggaa cgtcagagg atggggagg 
      agagcagta ttccggacg aagaggatt ccgttcaaa 
      tcattgctg atattttgt cgtccgtcc aggctccaa 
      aggtatctg aactgagtt cccgaagtg ccttattgc 
      tgtgggcgc cgcccacga aactctcgg aagcttcga 
      gtacggacg gccccgaga tcgacgtcg gtgatttac 
      gctacgaag tattactta gtgataaga gcaaagaaa 
      tgaaattta agtcaatat acagcggta cttcgctgt 
      cttgtcgca tccgggacc taaaaccgg gccaggagt 
      actccgtct gtctccagg tgcactacg acgagctcc 
      agggcgccg ccaccgacc ccgtcaagt tcaccacgc 
      cgccgtgcg agcccgacc agccgcagc cccccaagc 
      tgcaccagc ggcagaaaa cctcgctgc agctgaagt 
      ggaacagcg tcaacgaca acggctcgc acatcacgt 
      gctacatcc tggagtacg acgagggca aaggcggcg 
      acttcgtgg agttccacc gcgggagag tgaaacagt 
      acaccatac acaagttgc aaccagcga ccccgtacc 
      ggttcaggc tggcggcga tgaacgaga tagggaaga 
      gcgggtact cggaagtgg tcacttaca acacttgta 
      acaacccgc cgacgaaac cgccgcctc cggtgctgg 
      cagaggcct cgatcaatt acctccact tgcagtgga 
      gcaaaaggc ctagcgatg aggagttta cgcttcaga 
      tgaacgact cgaggacta gctatcggc cggtttaca 
      acggaaggg agacgattt gtatttgta cgggtctga 
      gtaatttta cggagtata agttccggc tgagggcgg 
      agaatgacg ggggacagt cgccgtggt ccgacgagg 
      tgacgtatc agaccttgc ctgatcgcc ccagggagc 
      cctccaagc ccgtcgtca agggccgga tccacgcac 
      actcgttca agctgaagt gggagccgc cgaactaca 
      ccggcggcg ccgacatca ccgagtata tcctcgagg 
      tgaactccg gcagcggct acgaggtcg tctacaccg 
      gaacggaaa cggaagcag tctgtgata aactaaccc 
      ccggcacca cctaccagt tgcgcgcga gctgcatca 
      gtgccggcg gccgtagca actactcaa acccttgca 
      ctgtgacga cggacgcca tcgcccccg gccagtgcg 
      ccgcccccc gtctgcacg gcaagccca agtcgagca 
      gcatcacca tcaggtggt gcgagcccg actacaacg 
      gcggggcgc cggtgctgg agtacgagg tggagatgg 
      cgggcccgc ccgaccaca cgcgaaatc tagttcaca 
      aaagcaaag agaccgaat gcacggctg cgaatctca 
      ctccgggct gtgagtacg agttctttg tgagagccg 
      tcaacagga tagggcccg gactgtggt cggatcctt 
      tgaaagtta ccagcggag cagctccgc cagatgtca 
      ttgagacgc tcgcgacca attgcaaat caccattcc 
      atatattcg tcgaatggc gagagccgc cgtctaacg 
      gagcgccca ttctagact atagattag aaattagca 
      caagtaata atgacttcc atttagttt atcaaggag 
      ccgaaaatt accatgacg tcaagggac tcagcccct 
      tcacgacgt actacttcc gggtgcaag catgcaact 
      ctgccgggt gcagtctgt actcccccg tggcggcga 
      ccgtcaccc ccgcagcgc ccccttcca gcgtaacga 
      ccctccgca gcgactcca cccccacgt caataacgc 
      tcaactgga acgagccct ccaataacg gttcggaaa 
      tcacccact acaatatcg aattcggtg ataagctca 
      tcgttactg acggtcccg tgagagagt atcaaatcg 
      acaatctcc aaccagaga caacctaca aaatcaaaa 
      tccaagccg taaatgcaa tcggcactg gctctcttt 
      cctccacgc ttaaagtat cgacactga aactgccgc 
      cagcagctc ctagacttg aatgcatcg gcattggac 
      acaactatc tcaaactga aatggggcg acggcaaaa 
      acgtcgact acactcact atctgatcg aaatggaaa 
      acggacgca cgcacgatt tccaatgcg tctataaag 
      gaacagctc tgacataca aagttaata aattgcatg 
      aactaacaa catataggt ttaggatct gcgcatcga 
      acgatgctg gtgtagggg atttttccg atatttatg 
      aatttacga ccagtatag caccgcctg cagtcgtca 
      aacaaccca aattgatcg aagtcgatc aaacgagct 
      gcgtgatcg aatggatgc cctccaaaa atcagttcg 
      tggatccgg tcatcttcc aagttcagg tggccagag 
      tcaaggacc agtttttca aagagacat acagaggcg 
      ctgagtcga agtgttcta tcgagaatt tagaatcag 
      gtgtggaat acagcgctc gtgtatgtc cgattagac 
      tgacggcca gcggcgagc tggtgggcc cctcttcgc 
      ctctcttgc acttttcga cactgattg ccgaaccga 
      ctgtagtat caaaaatct cgacaaatc ccagctcac 
      cgactcact cccaccgaa accaccgga ctgcactga 
      cgtcaattc tgcaccgct taaagacac tcctaaata 
      cgccccttg tcgttgcca tcgtggtaa caatcatag 
      gaattatca tttcgatat ggattatgt cgtttctct 
      aagtgcaat tgatgtgtg gtcgctttg ctcactcca 
      aacaaacaa acttaaact aagcgatac taccctgcc 
      gtttggcga cgaaaagtg gaatataaa gtattatta 
      aacagggct tattataat tagaggacc aaagcgcaa 
      gttttggta tttttatac gtgtttccg tacttggcg 
      tggtgctat ccaattaat taacgatgt gatgtctaa 
      cggcggtca ccgccgcag atgacctgt agtttgata 
      tatgtgttg ttattccag tcatgtgta tagtaaagc 
      aaacccgta cacatattg tatgtattt atgaagcag 
      gattaacta aatgaattt tcaagtggc tggaaagtc 
      tgtacctat aaaatttta aatatatat acttatata 
      atacagcat ataggttaa tgaaattat ataactcta 
      atgttaatc tattaagta tattacaat ctaatcttt 
      tattgttta aagttggct aaaattggg tttgtatat 
      gtgctactt cctgcacag tcgacagag atatattaa 
      tatattata ggtataaaa