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Gene information
General information
TC010452 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 6443392..6447049
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010452
    iBeetle screen iB_01695
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010452 RA
      MGKEKIHIN IVVIGHVDS GKSTSTGHL IYKCGGIDK 
      RTIEKFEKE AQEMGKGSF KYAWVLDKL KAERERGIT 
      IDIALWKFE TSKYYVTII DAPGHRDFI KNMITGTSQ 
      ADCAVLIVA AGTGEFEAG ISKNGQTRE HALLAFTLG 
      VKQLIVGVN KMDSTEPPY SEARFEEIK KEVSSYIKK 
      IGYNPAAVA FVPISGWHG DNMLEPSDK MPWFKGWSV 
      ERKEGKAEG KTLIEALDA ILPPSRPTE KPLRLPLQD 
      VYKIGGIGT VPVGRVETG VLKPGMVVT FAPVGLTTE 
      VKSVEMHHE ALQEAVPGD NVGFNVKNV SVKELRRGY 
      VAGDSKNNP PKGAADFTA QVIVLNHPG QIANGYTPV 
      LDCHTAHIA CKFAEIKEK CDRRTGKTT EENPKSIKS 
      GDAAIVNLV PSKPLCVES FQEFPPLGR FAVRDMRQT 
      VAVGVIKSV NFKDTTGKV TKAAEKAQK KK
    CDS sequence
    • >TC010452 RA
      atgggtaag gaaaagatc catattaac atcgtcgtg 
      attggtcac gtcgattcc ggcaagtcc acctcgact 
      ggtcatttg atttacaaa tgcggcggt atcgacaaa 
      cgtaccatc gagaaattc gagaaggag gcccaagaa 
      atgggcaag ggctctttc aaatacgcc tgggttttg 
      gacaagctc aaggccgag cgtgagcgt ggtatcacc 
      atcgacatc gcgctttgg aaattcgaa acctcgaaa 
      tactacgtt actatcatt gacgccccc ggccacagg 
      gatttcatc aagaacatg atcacggga acgtcgcag 
      gccgattgt gccgtctta atcgtagcc gcaggcact 
      ggcgaattc gaggcaggg atttcgaag aacggccaa 
      acgcgcgaa cacgccctt ttagcgttc actttgggc 
      gtgaaacag ctcattgtt ggagtgaat aagatggat 
      tcgaccgaa ccaccctat tccgaggcg cgattcgag 
      gaaatcaag aaggaagtc tcgtcgtac atcaagaag 
      atcgggtat aatcccgca gctgtggct tttgtcccc 
      atttcgggg tggcatggg gataatatg ctcgagccc 
      tctgataag atgccctgg ttcaagggg tggagcgtc 
      gagaggaag gaagggaag gctgaagga aagacgctg 
      atcgaggcg ctggatgcg atcttgccg ccgtcaagg 
      cccaccgag aaacctcta cgtcttccc cttcaggat 
      gtgtacaag attggtggt attggcacc gtgcccgtg 
      ggccgagtg gagacgggc gtgctgaag cccggcatg 
      gtggtgacg ttcgccccc gtgggcctg accactgaa 
      gtgaagtcg gtggagatg caccacgag gctctgcag 
      gaggcggtg cccggcgac aacgtcggt ttcaacgtg 
      aagaacgtg tccgtaaag gagctgcgt cgcggttac 
      gtggccgga gactcgaag aacaatcca cccaaagga 
      gctgccgac ttcaccgct caagtcatc gtcctgaac 
      catcctggc cagatcgcc aacggatac actccggta 
      ttagattgc cacactgct catatcgct tgcaagttt 
      gccgaaatc aaggagaag tgcgatcgc cgtacgggc 
      aagacgact gaggagaat ccgaagtcg atcaagtct 
      ggtgacgcc gccatcgtc aacttggtg ccgtctaag 
      ccactctgc gtcgagtcg ttccaagag tttccgccc 
      ttgggacgt ttcgctgtc cgcgatatg cggcaaact 
      gtggccgtc ggcgtcatc aagtctgtc aactttaaa 
      gatactact gggaaagta acgaaggcc gccgagaag 
      gcacaaaag aagaaataa
    mRNA sequence
    • >TC010452 RA
      taggagagg attgtcgtt tttcccgtt ctcttgctg 
      cagtttcag aggcaaaac tcgtaatcc gccatgggt 
      aaggaaaag atccatatt aacatcgtc gtgattggt 
      cacgtcgat tccggcaag tccacctcg actggtcat 
      ttgatttac aaatgcggc ggtatcgac aaacgtacc 
      atcgagaaa ttcgagaag gaggcccaa gaaatgggc 
      aagggctct ttcaaatac gcctgggtt ttggacaag 
      ctcaaggcc gagcgtgag cgtggtatc accatcgac 
      atcgcgctt tggaaattc gaaacctcg aaatactac 
      gttactatc attgacgcc cccggccac agggatttc 
      atcaagaac atgatcacg ggaacgtcg caggccgat 
      tgtgccgtc ttaatcgta gccgcaggc actggcgaa 
      ttcgaggca gggatttcg aagaacggc caaacgcgc 
      gaacacgcc cttttagcg ttcactttg ggcgtgaaa 
      cagctcatt gttggagtg aataagatg gattcgacc 
      gaaccaccc tattccgag gcgcgattc gaggaaatc 
      aagaaggaa gtctcgtcg tacatcaag aagatcggg 
      tataatccc gcagctgtg gcttttgtc cccatttcg 
      gggtggcat ggggataat atgctcgag ccctctgat 
      aagatgccc tggttcaag gggtggagc gtcgagagg 
      aaggaaggg aaggctgaa ggaaagacg ctgatcgag 
      gcgctggat gcgatcttg ccgccgtca aggcccacc 
      gagaaacct ctacgtctt ccccttcag gatgtgtac 
      aagattggt ggtattggc accgtgccc gtgggccga 
      gtggagacg ggcgtgctg aagcccggc atggtggtg 
      acgttcgcc cccgtgggc ctgaccact gaagtgaag 
      tcggtggag atgcaccac gaggctctg caggaggcg 
      gtgcccggc gacaacgtc ggtttcaac gtgaagaac 
      gtgtccgta aaggagctg cgtcgcggt tacgtggcc 
      ggagactcg aagaacaat ccacccaaa ggagctgcc 
      gacttcacc gctcaagtc atcgtcctg aaccatcct 
      ggccagatc gccaacgga tacactccg gtattagat 
      tgccacact gctcatatc gcttgcaag tttgccgaa 
      atcaaggag aagtgcgat cgccgtacg ggcaagacg 
      actgaggag aatccgaag tcgatcaag tctggtgac 
      gccgccatc gtcaacttg gtgccgtct aagccactc 
      tgcgtcgag tcgttccaa gagtttccg cccttggga 
      cgtttcgct gtccgcgat atgcggcaa actgtggcc 
      gtcggcgtc atcaagtct gtcaacttt aaagatact 
      actgggaaa gtaacgaag gccgccgag aaggcacaa 
      aagaagaaa taactaggc ttagttcag tcttctgcc 
      tcaggcccc aagaaaatc ttcatcagc aactattat 
      ttattattc tctaggtgg ctacagcag aattatttt 
      catcgcaaa gagttcaga aggaaaaac atttccttt 
      cattctcat tttaggttt ttatttaat tgaacaact 
      gtaatttat aattattta tgcaggaaa gggcatttc 
      tgatagaat ttaagtttt gtttctatt atttattgt 
      ttttttttg ttattattg atgatgata gacttgcaa 
      ttgatatgt aagcagctt ctgtaacaa atacccaat 
      aaagaagct caagataa