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Gene information
General information
TC010450 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 6430592..6433982
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010450
    iBeetle screen iB_01694
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Fertility
    egg production: number >150% of expected -
    Comment: No phenotype!
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010450 RA
      MSSYLDEIL KTLGWEDGF QIPVANAEN QQLEAEVAR 
      LTLQKIKEK TALETSAVK LENIQNHLK YVKQESEQN 
      QNLITAHAQ QLQSEESRL RLSAAEKDK FEQDIKQCE 
      KQFADIQAR QSIKKNQLE RYVKKLDKL KSETDWDSE 
      ALKAWEESL KKRDDDNEL IKKFSKQDD RKYNELEAR 
      RQNLEVEFA NRKQTIDKM VSDLINYER VLERTGKIM 
      KQQVVERNA LIQQWKESV NILHQRDND IDRLQAQML 
      ETQEIISKE EEKLEEQKQ FYENEVRNN NELQHEAQQ 
      LNLLNSRIR RELNEMSQN MLVLNSQAD TLRRTMILV 
      ANQLEKERL HKKHAWEEI EKMEHKIAI FRANLDVLK 
      TKNEQVKQS TLSADERMK HLEKLIDYE QKQQKTLQL 
      EAESLQNAY FRSQHELQE QQSIGKMRD LEIYGMTTN 
      INHLRKHND DQKKALGKR KEDAYDLDY RIDEVQRKI 
      LINENVLHS DDTEELEGK LIELENKIA EHTEIKNLL 
      KDQVNKIRD DMRRLTTAI VADQNQLSV LKDKLQHQN 
      LNFEGGQKQ LQVLKNGIQ EKQVEENIL RLRVNQLNK 
      AMKKEENSI YTMKKFKLT LEQATQERM LEIETSKDI 
      LLAKKRNLG EDNGRLRCD IAERRLKLD HFKKKYDII 
      LTSLGKDED GQPLSVTHF KIKNAQEKY LLQQEGDEL 
      DMKIKKAEK EILAMENTL KVVNLTNAA FKQSLGAVE 
      DDGREMSEM KMLENEMLL INDTLRENR KKMAVKRQE 
      LEEVQNALK ELEIKRDQA KDALFDLEE EYLKTEKES 
      SEKEEKLKR AERCLKLAQ KKVRHKERE KYDRDLEIR 
      QLKEANLSA MQRLVEMSI RYQEMAPLI TRYTLEYDV 
      KLPEKRTIA SLCACSEST RSTSSSSCN QEKDVSDVK 
      SVKSESSST KSSAISKVS ITFQM
    CDS sequence
    • >TC010450 RA
      atgtcgagc tatttagac gaaattttg aaaactcta 
      gggtgggaa gatggcttt caaatacca gtcgcgaat 
      gcggaaaat caacaactc gaagcggaa gtcgccaga 
      ctcacgctc caaaaaatc aaggagaaa acagccctt 
      gaaacgtcc gccgtcaag ctcgaaaac atccagaat 
      cacctcaaa tacgttaag caagaaagc gagcaaaat 
      cagaatctg atcactgca cacgcccaa caactccaa 
      tccgaggag agtagactc cgcttgagc gccgctgaa 
      aaggataag ttcgagcag gacataaaa cagtgcgag 
      aaacaattc gccgatatc caggcacga caatcaatc 
      aagaaaaac cagctggag agatatgtg aaaaaattg 
      gacaaactc aaatccgaa acggactgg gacagcgag 
      gctctgaaa gcttgggaa gagtcgcta aaaaagcgc 
      gatgacgac aatgaacta atcaaaaaa ttctccaaa 
      caagacgac cgaaagtac aacgaactg gaagccagg 
      cgccagaac ttggaagtc gaattcgcg aacagaaaa 
      caaaccatt gacaaaatg gtctctgat ctgattaat 
      tacgaacgt gtcttggag cgcaccgga aaaatcatg 
      aagcagcaa gtcgttgaa cgcaatgcc ctcatccag 
      cagtggaag gagtcggtg aacatcctg caccaacgc 
      gacaacgac attgaccgc ctccaagca caaatgctg 
      gaaacgcag gaaataatc tcgaaggag gaggagaaa 
      ctagaggaa cagaagcaa ttttacgaa aacgaagtg 
      cgaaacaac aacgaactg cagcacgaa gcgcaacag 
      ttgaacctc ctaaactcg cgaatccgt cgcgaattg 
      aacgaaatg tcccaaaac atgttggtt ttgaacagt 
      caagccgat actttgcga cgaaccatg attctggtg 
      gccaaccag ctggagaaa gaacgtctg cacaagaaa 
      cacgcttgg gaagaaatc gagaagatg gaacacaaa 
      attgccatt ttccgcgcc aatttagac gtgctcaaa 
      accaagaac gagcaagtg aaacagtcg actttgtcg 
      gccgatgaa cgcatgaag cacttggag aagttgatt 
      gattacgaa cagaagcaa cagaaaacg ttgcagctt 
      gaagctgag tcgctgcag aatgcgtat ttccggtcc 
      caacacgaa ctgcaggaa cagcaaagt attgggaaa 
      atgcgagat cttgagatt tatggcatg accacgaat 
      atcaatcac ttgcggaag cataacgac gaccaaaag 
      aaggcctta gggaagcga aaggaggac gcgtacgat 
      ttggactat aggattgat gaggttcaa aggaaaatt 
      ctgataaac gagaatgtg cttcacagt gatgatacg 
      gaagaactc gaagggaaa ctcatcgag ttggaaaac 
      aaaattgcg gagcatact gaaattaaa aatttgtta 
      aaagatcaa gtgaacaaa atccgagat gatatgcga 
      agactgacc acggcaatc gtagctgac cagaatcaa 
      ctaagcgtc ttaaaagac aaactgcaa caccagaac 
      ttgaatttt gaaggcggg caaaaacaa ttacaagtc 
      ctgaaaaac ggcatacaa gagaagcaa gtggaggag 
      aacatccta cgcctgcgt gtcaaccaa ttgaacaaa 
      gcgatgaag aaggaagaa aacagtatc tacaccatg 
      aaaaaattc aaattaact ttggaacaa gcgactcaa 
      gaacgcatg cttgaaatt gagaccagt aaagatatc 
      cttcttgcg aaaaaacgc aacttgggt gaagacaat 
      ggacgatta agatgcgat atcgccgaa aggcgacta 
      aaactggac catttcaag aaaaaatat gacattatt 
      ttgaccagt ttggggaaa gatgaggac ggacagcca 
      ctgtctgtt actcacttt aagattaag aatgcgcaa 
      gaaaagtat ttgttgcaa caggaagga gatgagcta 
      gatatgaag ataaaaaag gcagaaaag gaaattctt 
      gcgatggaa aacactttg aaggttgtc aatttgaca 
      aatgcggcc tttaagcaa agtcttgga gcggttgag 
      gatgatgga cgggaaatg agtgaaatg aaaatgttg 
      gaaaatgaa atgcttttg ataaatgat actttgcgg 
      gagaatagg aagaagatg gcagtgaaa cgacaagaa 
      ctggaagaa gtacaaaat gcactaaaa gagcttgaa 
      ataaaacgc gatcaagcc aaagacgca cttttcgac 
      ttggaggaa gagtactta aaaactgag aaagaaagc 
      tctgagaaa gaagaaaag ctcaaaaga gctgaacgt 
      tgtttaaaa ctcgcacaa aagaaagtt cgtcacaag 
      gaacgtgaa aaatatgac agagacttg gaaatccgc 
      caattgaag gaagctaat ctcagtgct atgcagcgt 
      ttggtggaa atgtctata cgttatcaa gaaatggca 
      ccgctgatt actcgttac acgctcgaa tatgacgtc 
      aaattaccc gaaaaacgc acaattgcc tccttgtgt 
      gcctgctca gaatcaaca aggtctacg tccagcagt 
      tcttgtaat caagaaaaa gacgtttct gacgttaaa 
      agcgtcaaa tctgagtcc agttcgacg aaaagttcc 
      gcaatttca aaagtctca attacattc caaatgtag
    mRNA sequence
    • >TC010450 RA
      ccatttcgc aacgatgtc gagctattt agacgaaat 
      tttgaaaac tctagggtg ggaagatgg ctttcaaat 
      accagtcgc gaatgcgga aaatcaaca actcgaagc 
      ggaagtcgc cagactcac gctccaaaa aatcaagga 
      gaaaacagc ccttgaaac gtccgccgt caagctcga 
      aaacatcca gaatcacct caaatacgt taagcaaga 
      aagcgagca aaatcagaa tctgatcac tgcacacgc 
      ccaacaact ccaatccga ggagagtag actccgctt 
      gagcgccgc tgaaaagga taagttcga gcaggacat 
      aaaacagtg cgagaaaca attcgccga tatccaggc 
      acgacaatc aatcaagaa aaaccagct ggagagata 
      tgtgaaaaa attggacaa actcaaatc cgaaacgga 
      ctgggacag cgaggctct gaaagcttg ggaagagtc 
      gctaaaaaa gcgcgatga cgacaatga actaatcaa 
      aaaattctc caaacaaga cgaccgaaa gtacaacga 
      actggaagc caggcgcca gaacttgga agtcgaatt 
      cgcgaacag aaaacaaac cattgacaa aatggtctc 
      tgatctgat taattacga acgtgtctt ggagcgcac 
      cggaaaaat catgaagca gcaagtcgt tgaacgcaa 
      tgccctcat ccagcagtg gaaggagtc ggtgaacat 
      cctgcacca acgcgacaa cgacattga ccgcctcca 
      agcacaaat gctggaaac gcaggaaat aatctcgaa 
      ggaggagga gaaactaga ggaacagaa gcaatttta 
      cgaaaacga agtgcgaaa caacaacga actgcagca 
      cgaagcgca acagttgaa cctcctaaa ctcgcgaat 
      ccgtcgcga attgaacga aatgtccca aaacatgtt 
      ggttttgaa cagtcaagc cgatacttt gcgacgaac 
      catgattct ggtggccaa ccagctgga gaaagaacg 
      tctgcacaa gaaacacgc ttgggaaga aatcgagaa 
      gatggaaca caaaattgc cattttccg cgccaattt 
      agacgtgct caaaaccaa gaacgagca agtgaaaca 
      gtcgacttt gtcggccga tgaacgcat gaagcactt 
      ggagaagtt gattgatta cgaacagaa gcaacagaa 
      aacgttgca gcttgaagc tgagtcgct gcagaatgc 
      gtatttccg gtcccaaca cgaactgca ggaacagca 
      aagtattgg gaaaatgcg agatcttga gatttatgg 
      catgaccac gaatatcaa tcacttgcg gaagcataa 
      cgacgacca aaagaaggc cttagggaa gcgaaagga 
      ggacgcgta cgatttgga ctataggat tgatgaggt 
      tcaaaggaa aattctgat aaacgagaa tgtgcttca 
      cagtgatga tacggaaga actcgaagg gaaactcat 
      cgagttgga aaacaaaat tgcggagca tactgaaat 
      taaaaattt gttaaaaga tcaagtgaa caaaatccg 
      agatgatat gcgaagact gaccacggc aatcgtagc 
      tgaccagaa tcaactaag cgtcttaaa agacaaact 
      gcaacacca gaacttgaa ttttgaagg cgggcaaaa 
      acaattaca agtcctgaa aaacggcat acaagagaa 
      gcaagtgga ggagaacat cctacgcct gcgtgtcaa 
      ccaattgaa caaagcgat gaagaagga agaaaacag 
      tatctacac catgaaaaa attcaaatt aactttgga 
      acaagcgac tcaagaacg catgcttga aattgagac 
      cagtaaaga tatccttct tgcgaaaaa acgcaactt 
      gggtgaaga caatggacg attaagatg cgatatcgc 
      cgaaaggcg actaaaact ggaccattt caagaaaaa 
      atatgacat tattttgac cagtttggg gaaagatga 
      ggacggaca gccactgtc tgttactca ctttaagat 
      taagaatgc gcaagaaaa gtatttgtt gcaacagga 
      aggagatga gctagatat gaagataaa aaaggcaga 
      aaaggaaat tcttgcgat ggaaaacac tttgaaggt 
      tgtcaattt gacaaatgc ggcctttaa gcaaagtct 
      tggagcggt tgaggatga tggacggga aatgagtga 
      aatgaaaat gttggaaaa tgaaatgct tttgataaa 
      tgatacttt gcgggagaa taggaagaa gatggcagt 
      gaaacgaca agaactgga agaagtaca aaatgcact 
      aaaagagct tgaaataaa acgcgatca agccaaaga 
      cgcactttt cgacttgga ggaagagta cttaaaaac 
      tgagaaaga aagctctga gaaagaaga aaagctcaa 
      aagagctga acgttgttt aaaactcgc acaaaagaa 
      agttcgtca caaggaacg tgaaaaata tgacagaga 
      cttggaaat ccgccaatt gaaggaagc taatctcag 
      tgctatgca gcgtttggt ggaaatgtc tatacgtta 
      tcaagaaat ggcaccgct gattactcg ttacacgct 
      cgaatatga cgtcaaatt acccgaaaa acgcacaat 
      tgcctcctt gtgtgcctg ctcagaatc aacaaggtc 
      tacgtccag cagttcttg taatcaaga aaaagacgt 
      ttctgacgt taaaagcgt caaatctga gtccagttc 
      gacgaaaag ttccgcaat ttcaaaagt ctcaattac 
      attccaaat gtaggtaaa aatacacaa ttaaagtaa 
      gg