Status
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Gene information
General information
TC010443 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7338840..7340772
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010443
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01693

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010443 RA
      MDKDNEPVG NCPICDKPF SLSLIESHV NKCIFLNSA 
DAEDMPKRK RSPSPILPQ NTTKPKTTQ KMDLVSPSK 
KPKTSSSTS TTKENPLTF TTPLAKQLQ PKCLDDFMG 
QSHVLGENT VLRTLLEKG DIPNMVLWG PPGCGKTSL 
SGVIQGICK SNPTKLKFV SLCAATAGV KDVQNIVSA 
AKLQQKFGC RTVLFMDEI HRFNKKQQD IFLLHVEKG 
DIILVGATT ENPSFTVNS ALLSRCRVI VLQKLDPDC 
LYQILERGA RNFNVEVVD KGARSKGFA VQADALKWL 
ADISDGDAR IALGNLQLV LQYNEDKNK VVTIEDIKE 
KIKKSHLLY DRKGEEHYN VISAMHKSI RGSDPNAAL 
YWTTRMIVS GEDPLYIAR RMVRAASED IGNADPRAL 
QLAVSTMQG CQLLGMPEC DVLLAQCAI YLARAPKSR 
EADSALARA KQLIKSCEG LQPSVPMHL RNAPTRLMK 
DLGYGKLEG DERYYMPPE LKNVDFFK
    CDS sequence
    • >TC010443 RA
      atggacaaa gataacgaa cccgttggt aactgccct 
atttgcgac aagcccttt tcgttaagc ttgatcgaa 
agtcacgtt aacaaatgt atcttcctg aactcggcg 
gacgctgaa gacatgccg aaacgcaaa cggtccccc 
tcgccaatt ctcccccag aacactacc aaaccaaag 
acaacccaa aaaatggat ttagtttca ccatccaag 
aaaccaaaa acctcgtct tcaacaagc accaccaaa 
gagaacccc ctgaccttt acaaccccc ttagccaag 
cagctccaa cccaagtgc ctggacgac ttcatgggc 
caaagccac gtcctgggc gaaaacacc gtgctgagg 
accctactg gagaaagga gacattccc aacatggtc 
ctgtggggg ccccccggc tgcggcaaa acgtccctc 
tcgggggtg atccaaggc atatgtaag agcaacccc 
acgaaatta aaattcgtg tcgttatgt gcggccact 
gcgggcgtt aaagacgtg caaaatata gtctcggca 
gcgaagctg cagcagaaa tttgggtgc cgcactgtt 
ttattcatg gatgagata cacaggttt aataaaaag 
cagcaggac attttcctg ttgcatgtg gagaaaggg 
gacattatt ttagtgggg gccaccact gaaaacccg 
tcgtttact gtaaattcg gcacttttg agccgctgt 
cgtgtgatt gtcttgcaa aagttggac cccgattgc 
ttgtatcaa attttggaa cggggggct cggaatttc 
aacgtggaa gttgtagat aagggggca aggagtaaa 
ggatttgcg gtgcaagca gacgcgctt aagtggttg 
gctgatata agcgatggg gacgccagg attgccctg 
gggaaccta caactagtt ctccaatac aatgaggat 
aaaaataaa gttgttacg atcgaggat attaaggaa 
aaaatcaag aaatctcat ttgttgtat gatcgcaaa 
ggggaagaa cactataat gttatttca gcaatgcac 
aagtcaatc aggggctca gaccccaat gcggccctt 
tactggacc acgagaatg atagtcagt ggtgaagac 
cctctctac attgctcgc agaatggtc agggctgct 
agtgaagat attggtaat gcagaccct agagcccta 
caactggca gtcagtaca atgcaaggg tgtcaattg 
ttagggatg cccgaatgt gacgtactt ttggcccag 
tgtgcaatt tatttagcc agagcccct aagtcaaga 
gaggccgat tcggcttta gctagagca aaacagctc 
attaaaagt tgtgaaggg ttgcaaccc tctgttcct 
atgcatttg agaaacgct ccaacaaga ttaatgaaa 
gatttgggg tatggaaaa cttgagggt gatgaacgg 
tattacatg cctccagaa ctcaaaaac gtggatttt 
ttcaagtaa
    mRNA sequence
    • >TC010443 RA
      catttaaaa ccaacgcgc gtattttcg aatgttcgt 
ttgtaggtt aagtttccc ccgctttgt tcgcaaaaa 
acccaacga tggacaaag ataacgaac ccgttggta 
actgcccta tttgcgaca agccctttt cgttaagct 
tgatcgaaa gtcacgtta acaaatgta tcttcctga 
actcggcgg acgctgaag acatgccga aacgcaaac 
ggtccccct cgccaattc tcccccaga acactacca 
aaccaaaga caacccaaa aaatggatt tagtttcac 
catccaaga aaccaaaaa cctcgtctt caacaagca 
ccaccaaag agaaccccc tgaccttta caaccccct 
tagccaagc agctccaac ccaagtgcc tggacgact 
tcatgggcc aaagccacg tcctgggcg aaaacaccg 
tgctgagga ccctactgg agaaaggag acattccca 
acatggtcc tgtgggggc cccccggct gcggcaaaa 
cgtccctct cgggggtga tccaaggca tatgtaaga 
gcaacccca cgaaattaa aattcgtgt cgttatgtg 
cggccactg cgggcgtta aagacgtgc aaaatatag 
tctcggcag cgaagctgc agcagaaat ttgggtgcc 
gcactgttt tattcatgg atgagatac acaggttta 
ataaaaagc agcaggaca ttttcctgt tgcatgtgg 
agaaagggg acattattt tagtggggg ccaccactg 
aaaacccgt cgtttactg taaattcgg cacttttga 
gccgctgtc gtgtgattg tcttgcaaa agttggacc 
ccgattgct tgtatcaaa ttttggaac ggggggctc 
ggaatttca acgtggaag ttgtagata agggggcaa 
ggagtaaag gatttgcgg tgcaagcag acgcgctta 
agtggttgg ctgatataa gcgatgggg acgccagga 
ttgccctgg ggaacctac aactagttc tccaataca 
atgaggata aaaataaag ttgttacga tcgaggata 
ttaaggaaa aaatcaaga aatctcatt tgttgtatg 
atcgcaaag gggaagaac actataatg ttatttcag 
caatgcaca agtcaatca ggggctcag accccaatg 
cggcccttt actggacca cgagaatga tagtcagtg 
gtgaagacc ctctctaca ttgctcgca gaatggtca 
gggctgcta gtgaagata ttggtaatg cagacccta 
gagccctac aactggcag tcagtacaa tgcaagggt 
gtcaattgt tagggatgc ccgaatgtg acgtacttt 
tggcccagt gtgcaattt atttagcca gagccccta 
agtcaagag aggccgatt cggctttag ctagagcaa 
aacagctca ttaaaagtt gtgaagggt tgcaaccct 
ctgttccta tgcatttga gaaacgctc caacaagat 
taatgaaag atttggggt atggaaaac ttgagggtg 
atgaacggt attacatgc ctccagaac tcaaaaacg 
tggattttt tcaagtaac ttcgccacc aaatgtttt 
ttacgttct ttatttatc aaatctgta atatacaga 
ctcctacaa tcactgtta ttgtaacag gaatataat 
aactaaaat ggattgttc aaaatataa atattttta 
cattaacta aaaccctta caaggtcgc ctaaagtaa 
tccagattt aaaaaaaaa tacatgagt tgttccttg 
ataaaacaa tcatttaaa aaaatgcac cacttccat 
caacgtttt caatttatt cacaataaa ttaatagtt 
aaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY