Status
Please wait ...
Gene information
General information
TC010443 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 6303632..6305564
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010443
    iBeetle screen iB_01693
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010443 RA
      MDKDNEPVG NCPICDKPF SLSLIESHV NKCIFLNSA 
      DAEDMPKRK RSPSPILPQ NTTKPKTTQ KMDLVSPSK 
      KPKTSSSTS TTKENPLTF TTPLAKQLQ PKCLDDFMG 
      QSHVLGENT VLRTLLEKG DIPNMVLWG PPGCGKTSL 
      SGVIQGICK SNPTKLKFV SLCAATAGV KDVQNIVSA 
      AKLQQKFGC RTVLFMDEI HRFNKKQQD IFLLHVEKG 
      DIILVGATT ENPSFTVNS ALLSRCRVI VLQKLDPDC 
      LYQILERGA RNFNVEVVD KGARSKGFA VQADALKWL 
      ADISDGDAR IALGNLQLV LQYNEDKNK VVTIEDIKE 
      KIKKSHLLY DRKGEEHYN VISAMHKSI RGSDPNAAL 
      YWTTRMIVS GEDPLYIAR RMVRAASED IGNADPRAL 
      QLAVSTMQG CQLLGMPEC DVLLAQCAI YLARAPKSR 
      EADSALARA KQLIKSCEG LQPSVPMHL RNAPTRLMK 
      DLGYGKLEG DERYYMPPE LKNVDFFK
    CDS sequence
    • >TC010443 RA
      atggacaaa gataacgaa cccgttggt aactgccct 
      atttgcgac aagcccttt tcgttaagc ttgatcgaa 
      agtcacgtt aacaaatgt atcttcctg aactcggcg 
      gacgctgaa gacatgccg aaacgcaaa cggtccccc 
      tcgccaatt ctcccccag aacactacc aaaccaaag 
      acaacccaa aaaatggat ttagtttca ccatccaag 
      aaaccaaaa acctcgtct tcaacaagc accaccaaa 
      gagaacccc ctgaccttt acaaccccc ttagccaag 
      cagctccaa cccaagtgc ctggacgac ttcatgggc 
      caaagccac gtcctgggc gaaaacacc gtgctgagg 
      accctactg gagaaagga gacattccc aacatggtc 
      ctgtggggg ccccccggc tgcggcaaa acgtccctc 
      tcgggggtg atccaaggc atatgtaag agcaacccc 
      acgaaatta aaattcgtg tcgttatgt gcggccact 
      gcgggcgtt aaagacgtg caaaatata gtctcggca 
      gcgaagctg cagcagaaa tttgggtgc cgcactgtt 
      ttattcatg gatgagata cacaggttt aataaaaag 
      cagcaggac attttcctg ttgcatgtg gagaaaggg 
      gacattatt ttagtgggg gccaccact gaaaacccg 
      tcgtttact gtaaattcg gcacttttg agccgctgt 
      cgtgtgatt gtcttgcaa aagttggac cccgattgc 
      ttgtatcaa attttggaa cggggggct cggaatttc 
      aacgtggaa gttgtagat aagggggca aggagtaaa 
      ggatttgcg gtgcaagca gacgcgctt aagtggttg 
      gctgatata agcgatggg gacgccagg attgccctg 
      gggaaccta caactagtt ctccaatac aatgaggat 
      aaaaataaa gttgttacg atcgaggat attaaggaa 
      aaaatcaag aaatctcat ttgttgtat gatcgcaaa 
      ggggaagaa cactataat gttatttca gcaatgcac 
      aagtcaatc aggggctca gaccccaat gcggccctt 
      tactggacc acgagaatg atagtcagt ggtgaagac 
      cctctctac attgctcgc agaatggtc agggctgct 
      agtgaagat attggtaat gcagaccct agagcccta 
      caactggca gtcagtaca atgcaaggg tgtcaattg 
      ttagggatg cccgaatgt gacgtactt ttggcccag 
      tgtgcaatt tatttagcc agagcccct aagtcaaga 
      gaggccgat tcggcttta gctagagca aaacagctc 
      attaaaagt tgtgaaggg ttgcaaccc tctgttcct 
      atgcatttg agaaacgct ccaacaaga ttaatgaaa 
      gatttgggg tatggaaaa cttgagggt gatgaacgg 
      tattacatg cctccagaa ctcaaaaac gtggatttt 
      ttcaagtaa
    mRNA sequence
    • >TC010443 RA
      catttaaaa ccaacgcgc gtattttcg aatgttcgt 
      ttgtaggtt aagtttccc ccgctttgt tcgcaaaaa 
      acccaacga tggacaaag ataacgaac ccgttggta 
      actgcccta tttgcgaca agccctttt cgttaagct 
      tgatcgaaa gtcacgtta acaaatgta tcttcctga 
      actcggcgg acgctgaag acatgccga aacgcaaac 
      ggtccccct cgccaattc tcccccaga acactacca 
      aaccaaaga caacccaaa aaatggatt tagtttcac 
      catccaaga aaccaaaaa cctcgtctt caacaagca 
      ccaccaaag agaaccccc tgaccttta caaccccct 
      tagccaagc agctccaac ccaagtgcc tggacgact 
      tcatgggcc aaagccacg tcctgggcg aaaacaccg 
      tgctgagga ccctactgg agaaaggag acattccca 
      acatggtcc tgtgggggc cccccggct gcggcaaaa 
      cgtccctct cgggggtga tccaaggca tatgtaaga 
      gcaacccca cgaaattaa aattcgtgt cgttatgtg 
      cggccactg cgggcgtta aagacgtgc aaaatatag 
      tctcggcag cgaagctgc agcagaaat ttgggtgcc 
      gcactgttt tattcatgg atgagatac acaggttta 
      ataaaaagc agcaggaca ttttcctgt tgcatgtgg 
      agaaagggg acattattt tagtggggg ccaccactg 
      aaaacccgt cgtttactg taaattcgg cacttttga 
      gccgctgtc gtgtgattg tcttgcaaa agttggacc 
      ccgattgct tgtatcaaa ttttggaac ggggggctc 
      ggaatttca acgtggaag ttgtagata agggggcaa 
      ggagtaaag gatttgcgg tgcaagcag acgcgctta 
      agtggttgg ctgatataa gcgatgggg acgccagga 
      ttgccctgg ggaacctac aactagttc tccaataca 
      atgaggata aaaataaag ttgttacga tcgaggata 
      ttaaggaaa aaatcaaga aatctcatt tgttgtatg 
      atcgcaaag gggaagaac actataatg ttatttcag 
      caatgcaca agtcaatca ggggctcag accccaatg 
      cggcccttt actggacca cgagaatga tagtcagtg 
      gtgaagacc ctctctaca ttgctcgca gaatggtca 
      gggctgcta gtgaagata ttggtaatg cagacccta 
      gagccctac aactggcag tcagtacaa tgcaagggt 
      gtcaattgt tagggatgc ccgaatgtg acgtacttt 
      tggcccagt gtgcaattt atttagcca gagccccta 
      agtcaagag aggccgatt cggctttag ctagagcaa 
      aacagctca ttaaaagtt gtgaagggt tgcaaccct 
      ctgttccta tgcatttga gaaacgctc caacaagat 
      taatgaaag atttggggt atggaaaac ttgagggtg 
      atgaacggt attacatgc ctccagaac tcaaaaacg 
      tggattttt tcaagtaac ttcgccacc aaatgtttt 
      ttacgttct ttatttatc aaatctgta atatacaga 
      ctcctacaa tcactgtta ttgtaacag gaatataat 
      aactaaaat ggattgttc aaaatataa atattttta 
      cattaacta aaaccctta caaggtcgc ctaaagtaa 
      tccagattt aaaaaaaaa tacatgagt tgttccttg 
      ataaaacaa tcatttaaa aaaatgcac cacttccat 
      caacgtttt caatttatt cacaataaa ttaatagtt 
      aaaac