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Gene information
General information
TC010437 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7296555..7301754
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010437
    iBeetle screen iB_01691

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010437 RA
      MSLLCGHKL LKTGFKNYS KLRRFTSEV TNSEVLVQY 
      FGLPSYKTN SYIKKQKLG RIRPENLIQ TANFCKEVG 
      FDLKTVLDA PMLLRFYPS TVDQYYTVL KEGGFKNIT 
      PKVLAKFRT LCRAPIYKL KNRHIDRKT DVVASFISH 
      LNPRPENFT VNTSGDEEV WAEAHFKVL LEYLKWRLN 
      ATDDDIAKL VKVHPTVCR KSFKYLCEN IATAEELGF 
      THDKILKYG YILHAYPKY TKEVMEKYP VVAGACIKR 
      AMRMYPKIV NTSPSNIKR ILQILRDHN ISDEAIAKR 
      MNVLHISPE TVDIRLHEL KNVADFKVL LHNPNILKL 
      VIHHNRAKS RLQFLKELQ LKCTTVSVL GNDNNKFDI 
      HIREGRDAN TFTDIYAFL KGTFKKEVD EFEATLKKH 
      PYYLQVPLL SMEDTYFYL REEKFSNSA IFKVIYILL 
      YPKEKIKNA FKEVNQFAS RRSKHLSQI SRLNLALYF 
      IEREHHFTG DGVWEKNER IEEAA
    CDS sequence
    • >TC010437 RA
      atgtcactc ttgtgtgga cataaactg ctaaagaca 
      ggctttaaa aactactcg aaattaagg agatttaca 
      agcgaagtt actaacagt gaagttcta gtacagtat 
      tttggtctg ccctcctat aaaacgaat tcatacatt 
      aagaagcaa aagcttggc cgaataaga ccggagaat 
      ctcatccaa acggcaaat ttctgtaaa gaagttggt 
      tttgacctt aaaactgtg ttagacgcc ccgatgctg 
      ttacgtttt tatcctagt acagtggac cagtactac 
      accgtatta aaggaaggg ggtttcaaa aacatcacc 
      cctaaggtt ttagccaaa tttagaacg ctttgccgc 
      gccccaata tataaacta aaaaatcga catatagat 
      cgaaaaaca gacgtagtt gctagtttt ataagtcat 
      ctaaacccg cgccctgaa aactttact gttaacact 
      tctggtgat gaagaggtc tgggctgaa gcacatttc 
      aaagtactg ttggaatat ttaaaatgg cgtttgaat 
      gctactgat gacgatatc gccaaattg gtcaaagta 
      catccaacc gtttgccgc aaaagcttc aaatattta 
      tgcgaaaac attgccact gcagaagaa ttaggtttc 
      acccacgac aaaatactg aaatatgga tacatactg 
      cacgcttat ccaaaatat accaaagaa gttatggaa 
      aagtatcca gttgtggca ggcgcttgt atcaagaga 
      gcgatgcgg atgtatccc aaaattgta aacacatct 
      ccctctaat attaagagg atcttacag attttaagg 
      gaccacaac atatcggac gaagcaatt gcaaaaaga 
      atgaatgtt ttgcatata tcgccagaa acggtggat 
      attcgttta catgagtta aaaaacgtg gccgacttc 
      aaagtgctt ttgcacaac cctaatatt ctcaaactt 
      gtaattcac cacaacagg gccaaatct cgtttacag 
      ttcctgaaa gagttgcaa ttaaaatgc acaactgtt 
      tcagtccta gggaatgat aataacaaa tttgatatt 
      catatcagg gaaggacgc gacgccaac acttttact 
      gacatttac gcgttttta aaggggact tttaagaaa 
      gaagttgac gagtttgaa gccaccttg aaaaaacac 
      ccttattat ttacaagtg cctttacta tcaatggaa 
      gatacgtat ttttacctt agggaagag aaattctca 
      aattctgcg atttttaaa gtcatttat attttgtta 
      tatcccaaa gagaaaatt aaaaatgcg ttcaaggag 
      gtgaaccag tttgctagt cgacgtagc aagcacttg 
      agccaaatc agccgatta aacctagct ttgtacttt 
      attgaaagg gaacaccat tttacaggg gatggcgtt 
      tgggaaaaa aatgaacgg atagaggaa gccgcttaa
    mRNA sequence
    • >TC010437 RA
      ccgtaaaag aataaattg ttgagtcat gaaaatctg 
      gcaaaacgt ttcttcacc gagctctga cctagggcg 
      tagtcaaaa atactactc cgagactaa ttttgaata 
      aacacaatt accatatca cataaaaaa tattggaat 
      gtacaggag ggactttag aacggtgtt catgattat 
      caaaacatt cgtaatcac ttaatcagt ctgcaagtt 
      tgcaagttt acctaaggc accagtgtt tccacaacc 
      attacgtgc attacaatt gtttttatt tttttattt 
      tcttaaatc agtaaatta ataataatc aatcaatgc 
      aacactttt ttgctacca tttacgttc aaaaaatct 
      tcacttttt aatttttaa attatcagt tcacaaact 
      actatttaa atgctatta taaagatct ttcaaatgt 
      ggaaacctc ggtagaaac tgatctgat cagtattat 
      ttcttaata ccgtatgac cgtatgaaa taaaacaat 
      tacaagggt actgtccat aaaattgaa atactgatc 
      atttagtat ttagtaatt gaaactaac atacaaatg 
      agggcaaat taatacaag attttttac aagttttaa 
      aaaaagctg ttggaaaat cctcaaaca acacacatt 
      aatacagtg tgtcattta aaaagcact gaagtgtcg 
      tcagtgttt ccaaaaaca taattttac taaagatta 
      ttgagtttg aaattttca acccacaaa atgtttgaa 
      aacacacca acatttggt aaaaacaat gcgccgcga 
      gtgcgtggt cctctgcca gccattgtg tgctgtacc 
      caaacatgg tactatgac ctggttcaa ttttaatta 
      aaataatta ccacagact ctaatgaat taatttagt 
      tacgatgaa cggggtgca tgtgactaa gaagaaaat 
      tcattgaaa tggcatcac ggtttttgc ttcgtgtgc 
      caatttcaa aagggcggc ccgagtgca gggaccaaa 
      cttatccta agttacgat gcgcacatc aatctttga 
      tcctttccg accagctgt tattccaac ccactgctt 
      atgtgacgt agtactgaa ccaaaattt aaaacgaaa 
      tcttaaaca ttgatttgt ttcgtaaac aatctcaat 
      taaaagtga acagataat cgctactgt gacaaatat 
      actaactat gaaagtgcc taaaataag aatgttaag 
      tcttagatt tttaatgaa tagaaataa atgtagttt 
      ttattaaag tgtagtgtc gcgctggtt gttttcatc 
      atgacttct taaagctct attattaaa atacagttt 
      tcgttccgt actacgccc gttaagctt caaggcttg 
      acaaacgaa ttatccgaa agcatcaag gagtttgcg 
      aagctctaa aagtgaaag tattactgc catgtttat 
      cagtcttat catctttta tctcgttct agtccgtag 
      ttttagcta attagtctt ttttagagc agtctttta 
      ggagccgta gatcctttc aaagtttgc ttcatctgt 
      tgactttcc gtagacgct gagtaaaaa tgacccacc 
      accagtgag accaaatac tttaagata ttttacctg 
      tacattgct tacggatag tattattcg aattaagag 
      tttgtacaa cttctgttg agaaaatca atttccata 
      cgaaaattt taaaagtat tatattact tattaaatt 
      ggtatagaa gcaaatttt acgaatata aaatatgtt 
      acaaatatt tgttgctat attgttaca tagaaaaca 
      aattgaaaa ttaagtgga agaaaaaaa taatgatcc 
      aacatagtt tttcgctat gcagaacaa agataaatg 
      aaaaataaa attgcaaaa actctccaa caactagtg 
      aaaccggtg caagtgata atggcatgt gatctaacc 
      tcaaaaaaa caactatga atatttttt aaatgtgat 
      caaaataac gatgtcact cttgtgtgg acataaact 
      gctaaagac aggctttaa aaactactc gaaattaag 
      gagatttac aagcgaagt tactaacag tgaagttct 
      agtacagta ttttggtct gccctccta taaaacgaa 
      ttcatacat taagaagca aaagcttgg ccgaataag 
      accggagaa tctcatcca aacggcaaa tttctgtaa 
      agaagttgg ttttgacct taaaactgt gttagacgc 
      cccgatgct gttacgttt ttatcctag tacagtgga 
      ccagtacta caccgtatt aaaggaagg gggtttcaa 
      aaacatcac ccctaaggt tttagccaa atttagaac 
      gctttgccg cgccccaat atataaact aaaaaatcg 
      acatataga tcgaaaaac agacgtagt tgctagttt 
      tataagtca tctaaaccc gcgccctga aaactttac 
      tgttaacac ttctggtga tgaagaggt ctgggctga 
      agcacattt caaagtact gttggaata tttaaaatg 
      gcgtttgaa tgctactga tgacgatat cgccaaatt 
      ggtcaaagt acatccaac cgtttgccg caaaagctt 
      caaatattt atgcgaaaa cattgccac tgcagaaga 
      attaggttt cacccacga caaaatact gaaatatgg 
      atacatact gcacgctta tccaaaata taccaaaga 
      agttatgga aaagtatcc agttgtggc aggcgcttg 
      tatcaagag agcgatgcg gatgtatcc caaaattgt 
      aaacacatc tccctctaa tattaagag gatcttaca 
      gattttaag ggaccacaa catatcgga cgaagcaat 
      tgcaaaaag aatgaatgt tttgcatat atcgccaga 
      aacggtgga tattcgttt acatgagtt aaaaaacgt 
      ggccgactt caaagtgct tttgcacaa ccctaatat 
      tctcaaact tgtaattca ccacaacag ggccaaatc 
      tcgtttaca gttcctgaa agagttgca attaaaatg 
      cacaactgt ttcagtcct agggaatga taataacaa 
      atttgatat tcatatcag ggaaggacg cgacgccaa 
      cacttttac tgacattta cgcgttttt aaaggggac 
      ttttaagaa agaagttga cgagtttga agccacctt 
      gaaaaaaca cccttatta tttacaagt gcctttact 
      atcaatgga agatacgta tttttacct tagggaaga 
      gaaattctc aaattctgc gatttttaa agtcattta 
      tattttgtt atatcccaa agagaaaat taaaaatgc 
      gttcaagga ggtgaacca gtttgctag tcgacgtag 
      caagcactt gagccaaat cagccgatt aaacctagc 
      tttgtactt tattgaaag ggaacacca ttttacagg 
      ggatggcgt ttgggaaaa aaatgaacg gatagagga 
      agccgctta aatacgaaa cgtgttgtt ctttattaa 
      ttttaatat aaatctgtt ttctttcct agtgttgtg 
      gggaaaaaa gaaaagtac ggaacatga atttattac 
      ctaaataaa taaatacac ggtgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY