| General information | |||
| TC010437 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 7296555..7301754 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC010437 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01691 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 4 (11 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC010437 RA
MSLLCGHKL LKTGFKNYS KLRRFTSEV TNSEVLVQY FGLPSYKTN SYIKKQKLG RIRPENLIQ TANFCKEVG FDLKTVLDA PMLLRFYPS TVDQYYTVL KEGGFKNIT PKVLAKFRT LCRAPIYKL KNRHIDRKT DVVASFISH LNPRPENFT VNTSGDEEV WAEAHFKVL LEYLKWRLN ATDDDIAKL VKVHPTVCR KSFKYLCEN IATAEELGF THDKILKYG YILHAYPKY TKEVMEKYP VVAGACIKR AMRMYPKIV NTSPSNIKR ILQILRDHN ISDEAIAKR MNVLHISPE TVDIRLHEL KNVADFKVL LHNPNILKL VIHHNRAKS RLQFLKELQ LKCTTVSVL GNDNNKFDI HIREGRDAN TFTDIYAFL KGTFKKEVD EFEATLKKH PYYLQVPLL SMEDTYFYL REEKFSNSA IFKVIYILL YPKEKIKNA FKEVNQFAS RRSKHLSQI SRLNLALYF IEREHHFTG DGVWEKNER IEEAA
- >TC010437 RA
atgtcactc ttgtgtgga cataaactg ctaaagaca ggctttaaa aactactcg aaattaagg agatttaca agcgaagtt actaacagt gaagttcta gtacagtat tttggtctg ccctcctat aaaacgaat tcatacatt aagaagcaa aagcttggc cgaataaga ccggagaat ctcatccaa acggcaaat ttctgtaaa gaagttggt tttgacctt aaaactgtg ttagacgcc ccgatgctg ttacgtttt tatcctagt acagtggac cagtactac accgtatta aaggaaggg ggtttcaaa aacatcacc cctaaggtt ttagccaaa tttagaacg ctttgccgc gccccaata tataaacta aaaaatcga catatagat cgaaaaaca gacgtagtt gctagtttt ataagtcat ctaaacccg cgccctgaa aactttact gttaacact tctggtgat gaagaggtc tgggctgaa gcacatttc aaagtactg ttggaatat ttaaaatgg cgtttgaat gctactgat gacgatatc gccaaattg gtcaaagta catccaacc gtttgccgc aaaagcttc aaatattta tgcgaaaac attgccact gcagaagaa ttaggtttc acccacgac aaaatactg aaatatgga tacatactg cacgcttat ccaaaatat accaaagaa gttatggaa aagtatcca gttgtggca ggcgcttgt atcaagaga gcgatgcgg atgtatccc aaaattgta aacacatct ccctctaat attaagagg atcttacag attttaagg gaccacaac atatcggac gaagcaatt gcaaaaaga atgaatgtt ttgcatata tcgccagaa acggtggat attcgttta catgagtta aaaaacgtg gccgacttc aaagtgctt ttgcacaac cctaatatt ctcaaactt gtaattcac cacaacagg gccaaatct cgtttacag ttcctgaaa gagttgcaa ttaaaatgc acaactgtt tcagtccta gggaatgat aataacaaa tttgatatt catatcagg gaaggacgc gacgccaac acttttact gacatttac gcgttttta aaggggact tttaagaaa gaagttgac gagtttgaa gccaccttg aaaaaacac ccttattat ttacaagtg cctttacta tcaatggaa gatacgtat ttttacctt agggaagag aaattctca aattctgcg atttttaaa gtcatttat attttgtta tatcccaaa gagaaaatt aaaaatgcg ttcaaggag gtgaaccag tttgctagt cgacgtagc aagcacttg agccaaatc agccgatta aacctagct ttgtacttt attgaaagg gaacaccat tttacaggg gatggcgtt tgggaaaaa aatgaacgg atagaggaa gccgcttaa
- >TC010437 RA
ccgtaaaag aataaattg ttgagtcat gaaaatctg gcaaaacgt ttcttcacc gagctctga cctagggcg tagtcaaaa atactactc cgagactaa ttttgaata aacacaatt accatatca cataaaaaa tattggaat gtacaggag ggactttag aacggtgtt catgattat caaaacatt cgtaatcac ttaatcagt ctgcaagtt tgcaagttt acctaaggc accagtgtt tccacaacc attacgtgc attacaatt gtttttatt tttttattt tcttaaatc agtaaatta ataataatc aatcaatgc aacactttt ttgctacca tttacgttc aaaaaatct tcacttttt aatttttaa attatcagt tcacaaact actatttaa atgctatta taaagatct ttcaaatgt ggaaacctc ggtagaaac tgatctgat cagtattat ttcttaata ccgtatgac cgtatgaaa taaaacaat tacaagggt actgtccat aaaattgaa atactgatc atttagtat ttagtaatt gaaactaac atacaaatg agggcaaat taatacaag attttttac aagttttaa aaaaagctg ttggaaaat cctcaaaca acacacatt aatacagtg tgtcattta aaaagcact gaagtgtcg tcagtgttt ccaaaaaca taattttac taaagatta ttgagtttg aaattttca acccacaaa atgtttgaa aacacacca acatttggt aaaaacaat gcgccgcga gtgcgtggt cctctgcca gccattgtg tgctgtacc caaacatgg tactatgac ctggttcaa ttttaatta aaataatta ccacagact ctaatgaat taatttagt tacgatgaa cggggtgca tgtgactaa gaagaaaat tcattgaaa tggcatcac ggtttttgc ttcgtgtgc caatttcaa aagggcggc ccgagtgca gggaccaaa cttatccta agttacgat gcgcacatc aatctttga tcctttccg accagctgt tattccaac ccactgctt atgtgacgt agtactgaa ccaaaattt aaaacgaaa tcttaaaca ttgatttgt ttcgtaaac aatctcaat taaaagtga acagataat cgctactgt gacaaatat actaactat gaaagtgcc taaaataag aatgttaag tcttagatt tttaatgaa tagaaataa atgtagttt ttattaaag tgtagtgtc gcgctggtt gttttcatc atgacttct taaagctct attattaaa atacagttt tcgttccgt actacgccc gttaagctt caaggcttg acaaacgaa ttatccgaa agcatcaag gagtttgcg aagctctaa aagtgaaag tattactgc catgtttat cagtcttat catctttta tctcgttct agtccgtag ttttagcta attagtctt ttttagagc agtctttta ggagccgta gatcctttc aaagtttgc ttcatctgt tgactttcc gtagacgct gagtaaaaa tgacccacc accagtgag accaaatac tttaagata ttttacctg tacattgct tacggatag tattattcg aattaagag tttgtacaa cttctgttg agaaaatca atttccata cgaaaattt taaaagtat tatattact tattaaatt ggtatagaa gcaaatttt acgaatata aaatatgtt acaaatatt tgttgctat attgttaca tagaaaaca aattgaaaa ttaagtgga agaaaaaaa taatgatcc aacatagtt tttcgctat gcagaacaa agataaatg aaaaataaa attgcaaaa actctccaa caactagtg aaaccggtg caagtgata atggcatgt gatctaacc tcaaaaaaa caactatga atatttttt aaatgtgat caaaataac gatgtcact cttgtgtgg acataaact gctaaagac aggctttaa aaactactc gaaattaag gagatttac aagcgaagt tactaacag tgaagttct agtacagta ttttggtct gccctccta taaaacgaa ttcatacat taagaagca aaagcttgg ccgaataag accggagaa tctcatcca aacggcaaa tttctgtaa agaagttgg ttttgacct taaaactgt gttagacgc cccgatgct gttacgttt ttatcctag tacagtgga ccagtacta caccgtatt aaaggaagg gggtttcaa aaacatcac ccctaaggt tttagccaa atttagaac gctttgccg cgccccaat atataaact aaaaaatcg acatataga tcgaaaaac agacgtagt tgctagttt tataagtca tctaaaccc gcgccctga aaactttac tgttaacac ttctggtga tgaagaggt ctgggctga agcacattt caaagtact gttggaata tttaaaatg gcgtttgaa tgctactga tgacgatat cgccaaatt ggtcaaagt acatccaac cgtttgccg caaaagctt caaatattt atgcgaaaa cattgccac tgcagaaga attaggttt cacccacga caaaatact gaaatatgg atacatact gcacgctta tccaaaata taccaaaga agttatgga aaagtatcc agttgtggc aggcgcttg tatcaagag agcgatgcg gatgtatcc caaaattgt aaacacatc tccctctaa tattaagag gatcttaca gattttaag ggaccacaa catatcgga cgaagcaat tgcaaaaag aatgaatgt tttgcatat atcgccaga aacggtgga tattcgttt acatgagtt aaaaaacgt ggccgactt caaagtgct tttgcacaa ccctaatat tctcaaact tgtaattca ccacaacag ggccaaatc tcgtttaca gttcctgaa agagttgca attaaaatg cacaactgt ttcagtcct agggaatga taataacaa atttgatat tcatatcag ggaaggacg cgacgccaa cacttttac tgacattta cgcgttttt aaaggggac ttttaagaa agaagttga cgagtttga agccacctt gaaaaaaca cccttatta tttacaagt gcctttact atcaatgga agatacgta tttttacct tagggaaga gaaattctc aaattctgc gatttttaa agtcattta tattttgtt atatcccaa agagaaaat taaaaatgc gttcaagga ggtgaacca gtttgctag tcgacgtag caagcactt gagccaaat cagccgatt aaacctagc tttgtactt tattgaaag ggaacacca ttttacagg ggatggcgt ttgggaaaa aaatgaacg gatagagga agccgctta aatacgaaa cgtgttgtt ctttattaa ttttaatat aaatctgtt ttctttcct agtgttgtg gggaaaaaa gaaaagtac ggaacatga atttattac ctaaataaa taaatacac ggtgt
