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Gene information
General information
TC010437 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7296555..7301754
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010437
    iBeetle screen iB_01691

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010437 RA
      MSLLCGHKL LKTGFKNYS KLRRFTSEV TNSEVLVQY 
FGLPSYKTN SYIKKQKLG RIRPENLIQ TANFCKEVG 
FDLKTVLDA PMLLRFYPS TVDQYYTVL KEGGFKNIT 
PKVLAKFRT LCRAPIYKL KNRHIDRKT DVVASFISH 
LNPRPENFT VNTSGDEEV WAEAHFKVL LEYLKWRLN 
ATDDDIAKL VKVHPTVCR KSFKYLCEN IATAEELGF 
THDKILKYG YILHAYPKY TKEVMEKYP VVAGACIKR 
AMRMYPKIV NTSPSNIKR ILQILRDHN ISDEAIAKR 
MNVLHISPE TVDIRLHEL KNVADFKVL LHNPNILKL 
VIHHNRAKS RLQFLKELQ LKCTTVSVL GNDNNKFDI 
HIREGRDAN TFTDIYAFL KGTFKKEVD EFEATLKKH 
PYYLQVPLL SMEDTYFYL REEKFSNSA IFKVIYILL 
YPKEKIKNA FKEVNQFAS RRSKHLSQI SRLNLALYF 
IEREHHFTG DGVWEKNER IEEAA
    CDS sequence
    • >TC010437 RA
      atgtcactc ttgtgtgga cataaactg ctaaagaca 
ggctttaaa aactactcg aaattaagg agatttaca 
agcgaagtt actaacagt gaagttcta gtacagtat 
tttggtctg ccctcctat aaaacgaat tcatacatt 
aagaagcaa aagcttggc cgaataaga ccggagaat 
ctcatccaa acggcaaat ttctgtaaa gaagttggt 
tttgacctt aaaactgtg ttagacgcc ccgatgctg 
ttacgtttt tatcctagt acagtggac cagtactac 
accgtatta aaggaaggg ggtttcaaa aacatcacc 
cctaaggtt ttagccaaa tttagaacg ctttgccgc 
gccccaata tataaacta aaaaatcga catatagat 
cgaaaaaca gacgtagtt gctagtttt ataagtcat 
ctaaacccg cgccctgaa aactttact gttaacact 
tctggtgat gaagaggtc tgggctgaa gcacatttc 
aaagtactg ttggaatat ttaaaatgg cgtttgaat 
gctactgat gacgatatc gccaaattg gtcaaagta 
catccaacc gtttgccgc aaaagcttc aaatattta 
tgcgaaaac attgccact gcagaagaa ttaggtttc 
acccacgac aaaatactg aaatatgga tacatactg 
cacgcttat ccaaaatat accaaagaa gttatggaa 
aagtatcca gttgtggca ggcgcttgt atcaagaga 
gcgatgcgg atgtatccc aaaattgta aacacatct 
ccctctaat attaagagg atcttacag attttaagg 
gaccacaac atatcggac gaagcaatt gcaaaaaga 
atgaatgtt ttgcatata tcgccagaa acggtggat 
attcgttta catgagtta aaaaacgtg gccgacttc 
aaagtgctt ttgcacaac cctaatatt ctcaaactt 
gtaattcac cacaacagg gccaaatct cgtttacag 
ttcctgaaa gagttgcaa ttaaaatgc acaactgtt 
tcagtccta gggaatgat aataacaaa tttgatatt 
catatcagg gaaggacgc gacgccaac acttttact 
gacatttac gcgttttta aaggggact tttaagaaa 
gaagttgac gagtttgaa gccaccttg aaaaaacac 
ccttattat ttacaagtg cctttacta tcaatggaa 
gatacgtat ttttacctt agggaagag aaattctca 
aattctgcg atttttaaa gtcatttat attttgtta 
tatcccaaa gagaaaatt aaaaatgcg ttcaaggag 
gtgaaccag tttgctagt cgacgtagc aagcacttg 
agccaaatc agccgatta aacctagct ttgtacttt 
attgaaagg gaacaccat tttacaggg gatggcgtt 
tgggaaaaa aatgaacgg atagaggaa gccgcttaa
    mRNA sequence
    • >TC010437 RA
      ccgtaaaag aataaattg ttgagtcat gaaaatctg 
gcaaaacgt ttcttcacc gagctctga cctagggcg 
tagtcaaaa atactactc cgagactaa ttttgaata 
aacacaatt accatatca cataaaaaa tattggaat 
gtacaggag ggactttag aacggtgtt catgattat 
caaaacatt cgtaatcac ttaatcagt ctgcaagtt 
tgcaagttt acctaaggc accagtgtt tccacaacc 
attacgtgc attacaatt gtttttatt tttttattt 
tcttaaatc agtaaatta ataataatc aatcaatgc 
aacactttt ttgctacca tttacgttc aaaaaatct 
tcacttttt aatttttaa attatcagt tcacaaact 
actatttaa atgctatta taaagatct ttcaaatgt 
ggaaacctc ggtagaaac tgatctgat cagtattat 
ttcttaata ccgtatgac cgtatgaaa taaaacaat 
tacaagggt actgtccat aaaattgaa atactgatc 
atttagtat ttagtaatt gaaactaac atacaaatg 
agggcaaat taatacaag attttttac aagttttaa 
aaaaagctg ttggaaaat cctcaaaca acacacatt 
aatacagtg tgtcattta aaaagcact gaagtgtcg 
tcagtgttt ccaaaaaca taattttac taaagatta 
ttgagtttg aaattttca acccacaaa atgtttgaa 
aacacacca acatttggt aaaaacaat gcgccgcga 
gtgcgtggt cctctgcca gccattgtg tgctgtacc 
caaacatgg tactatgac ctggttcaa ttttaatta 
aaataatta ccacagact ctaatgaat taatttagt 
tacgatgaa cggggtgca tgtgactaa gaagaaaat 
tcattgaaa tggcatcac ggtttttgc ttcgtgtgc 
caatttcaa aagggcggc ccgagtgca gggaccaaa 
cttatccta agttacgat gcgcacatc aatctttga 
tcctttccg accagctgt tattccaac ccactgctt 
atgtgacgt agtactgaa ccaaaattt aaaacgaaa 
tcttaaaca ttgatttgt ttcgtaaac aatctcaat 
taaaagtga acagataat cgctactgt gacaaatat 
actaactat gaaagtgcc taaaataag aatgttaag 
tcttagatt tttaatgaa tagaaataa atgtagttt 
ttattaaag tgtagtgtc gcgctggtt gttttcatc 
atgacttct taaagctct attattaaa atacagttt 
tcgttccgt actacgccc gttaagctt caaggcttg 
acaaacgaa ttatccgaa agcatcaag gagtttgcg 
aagctctaa aagtgaaag tattactgc catgtttat 
cagtcttat catctttta tctcgttct agtccgtag 
ttttagcta attagtctt ttttagagc agtctttta 
ggagccgta gatcctttc aaagtttgc ttcatctgt 
tgactttcc gtagacgct gagtaaaaa tgacccacc 
accagtgag accaaatac tttaagata ttttacctg 
tacattgct tacggatag tattattcg aattaagag 
tttgtacaa cttctgttg agaaaatca atttccata 
cgaaaattt taaaagtat tatattact tattaaatt 
ggtatagaa gcaaatttt acgaatata aaatatgtt 
acaaatatt tgttgctat attgttaca tagaaaaca 
aattgaaaa ttaagtgga agaaaaaaa taatgatcc 
aacatagtt tttcgctat gcagaacaa agataaatg 
aaaaataaa attgcaaaa actctccaa caactagtg 
aaaccggtg caagtgata atggcatgt gatctaacc 
tcaaaaaaa caactatga atatttttt aaatgtgat 
caaaataac gatgtcact cttgtgtgg acataaact 
gctaaagac aggctttaa aaactactc gaaattaag 
gagatttac aagcgaagt tactaacag tgaagttct 
agtacagta ttttggtct gccctccta taaaacgaa 
ttcatacat taagaagca aaagcttgg ccgaataag 
accggagaa tctcatcca aacggcaaa tttctgtaa 
agaagttgg ttttgacct taaaactgt gttagacgc 
cccgatgct gttacgttt ttatcctag tacagtgga 
ccagtacta caccgtatt aaaggaagg gggtttcaa 
aaacatcac ccctaaggt tttagccaa atttagaac 
gctttgccg cgccccaat atataaact aaaaaatcg 
acatataga tcgaaaaac agacgtagt tgctagttt 
tataagtca tctaaaccc gcgccctga aaactttac 
tgttaacac ttctggtga tgaagaggt ctgggctga 
agcacattt caaagtact gttggaata tttaaaatg 
gcgtttgaa tgctactga tgacgatat cgccaaatt 
ggtcaaagt acatccaac cgtttgccg caaaagctt 
caaatattt atgcgaaaa cattgccac tgcagaaga 
attaggttt cacccacga caaaatact gaaatatgg 
atacatact gcacgctta tccaaaata taccaaaga 
agttatgga aaagtatcc agttgtggc aggcgcttg 
tatcaagag agcgatgcg gatgtatcc caaaattgt 
aaacacatc tccctctaa tattaagag gatcttaca 
gattttaag ggaccacaa catatcgga cgaagcaat 
tgcaaaaag aatgaatgt tttgcatat atcgccaga 
aacggtgga tattcgttt acatgagtt aaaaaacgt 
ggccgactt caaagtgct tttgcacaa ccctaatat 
tctcaaact tgtaattca ccacaacag ggccaaatc 
tcgtttaca gttcctgaa agagttgca attaaaatg 
cacaactgt ttcagtcct agggaatga taataacaa 
atttgatat tcatatcag ggaaggacg cgacgccaa 
cacttttac tgacattta cgcgttttt aaaggggac 
ttttaagaa agaagttga cgagtttga agccacctt 
gaaaaaaca cccttatta tttacaagt gcctttact 
atcaatgga agatacgta tttttacct tagggaaga 
gaaattctc aaattctgc gatttttaa agtcattta 
tattttgtt atatcccaa agagaaaat taaaaatgc 
gttcaagga ggtgaacca gtttgctag tcgacgtag 
caagcactt gagccaaat cagccgatt aaacctagc 
tttgtactt tattgaaag ggaacacca ttttacagg 
ggatggcgt ttgggaaaa aaatgaacg gatagagga 
agccgctta aatacgaaa cgtgttgtt ctttattaa 
ttttaatat aaatctgtt ttctttcct agtgttgtg 
gggaaaaaa gaaaagtac ggaacatga atttattac 
ctaaataaa taaatacac ggtgt
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