| General information | |||
| TC010434 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 7301756..7313233 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC010434 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01690 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Adult morphology
potentially confused/crosscontaminated with : eggs of..., iB # (comma separated) 01783
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: > 50
larval musculature irregular -
Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
Analysis of larval stage 1 cuticle
potentially confused/crosscontaminated with : eggs of..., iB # (comma separated) 01783
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
Days post injection
adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
Days post injection
- >TC010434 RA
MDSALKQSR KPNPRENAF PLSAATFWY TIPTFVQGF KRDLEESDL TETLTEHKS SILGNKMEK AWKAEEIKA AKANRKPSL ERVLFKVFS FEFVFYGIV LALSEAIRL NQPLFLGKF VAFFDTYRT PVMRDAAHV VTDGTAQNH SEEIGQPLA LGKLLTFYQ PLNTEVSQT DAYWYAAGV VICSLANIA FSHPQMLGV MHLGMKMRV ACCSLIYRK TLKLSKTAL GQTTAGQVV NLLSNDVNR FDVALLFAH QLWVGPLET IVCTYFMYL QVGYSAIIG VAFLLLFIP LQIFLGKRI SVLRLRTAL RTDERVRLM NEIISGIQV IKMYAWEKP FASLVALAR RYEIKSIRI SSYMRGITL SFIMFTTRM SIFASVLAY VLFDNTITA EKVFVLTSF YNILRQTMT VFFPQGISQ VAEARVSIA RLNKFMLYD ETQIAKELK RRQAEGKKD NLISNGIDA ARDLGVFMK NASAKWSEA SSDNTLNNV NLTAVPGKL VAVIGPVGS GKSSLFHAI LQELPLFDG SLSVNGEIS YASQEPWLF AGSVRQNIL FGLPMDKLR YKTVVKKCA LERDFTLLP YGDKTMVGD RGVSLSGGQ RARINLARA VYKQADIYL LDDPLSAVD THVGKQLFE NCIAGYLKN KTVILITHQ LQYLKEVDQ IIYLHDGVV KAQGSFKEL QATGLDFTN LLGAAQDED EEKKKEEEL IRQGSIRSI ASVEGEAPK IVEEQKGTG SVGADVYLG YFKAGGNCC VIFVLFALF IVTQIFASI ADYFITYWV NIEQQDAQK NKTSVAEAQ DDDFWHFSR DTSIYIYSV IIGLLIIIT LIRSFTFFS VCMRASTRL HDNMFASIT RATMRFFNT NSAGRILNR FSKDMGSID ELLTSAMID CLQIGLSLL GIIIVVAVV SPWLMVPTV VAGIIFYFL RIFYIRTSR NVKRLEGIT RSPVFSHLN ASLQGLTTI RAFGAQEIL EKEFDGHQD LHSSAWFSF ISTSRAFGY WLDVVCIIY ITLVTFSFL VIGNEKFGG NVGLAITQA IGLTGMFQW GMRQSTELE NQMTSVERV LEYNNIEHE GNLESPPDK KPAPSWPND GKIEFINVF LRYFPDDPP VLKNLSFTI NPREKIGIV GRTGAGKSS LINAIFQLS DTQGAIIID GIDITEIGL HDLRSKISI IPQEPVLFS GTMRKNLDP FDDYSDADL WRALEDVEL KDEVSNLTS GLNSKMSEG GSNFSVGQR QLVCLARAI LRNNKILVL DEATANIDP QTDALIQNT IRNKFSDCT VLTIAHRLH TVMDSDKIL VMDAGTMKE FDHAYNLLQ DSNTILYGM VQQTGKAMA ETLFNVAKE SYNKIHQG
- >TC010434 RB
MDSALKQSR KPNPRENAF PLSAATFWY TIPTFVQGF KRDLEESDL TETLTEHKS SILGNKMEK AWKAEEIKA AKANRKPSL ERVLFKVFS FEFVFYGIV LALSEAIRI GQPLALGKL LTFYQPLNT EVSQTDAYW YAAGVVICS LANIAFSHP QMLGVMHLG MKMRVACCS LIYRKTLKL SKTALGQTT AGQVVNLLS NDVNRFDVA LLFAHQLWV GPLETIVCT YFMYLQVGY SAIIGVAFL LLFIPLQIF LGKRISVLR LRTALRTDE RVRLMNEII SGIQVIKMY AWEKPFASL VALARRYEI KSIRISSYM RGITLSFIM FTTRMSIFA SVLAYVLFD NTITAEKVF VLTSFYNIL RQTMTVFFP QGISQVAEA RVSIARLNK FMLYDETQI AKELKRRQA EGKKDNLIS NGIDAARDL GVFMKNASA KWSEASSDN TLNNVNLTA VPGKLVAVI GPVGSGKSS LFHAILQEL PLFDGSLSV NGEISYASQ EPWLFAGSV RQNILFGLP MDKLRYKTV VKKCALERD FTLLPYGDK TMVGDRGVS LSGGQRARI NLARAVYKQ ADIYLLDDP LSAVDTHVG KQLFENCIA GYLKNKTVI LITHQLQYL KEVDQIIYL HDGVVKAQG SFKELQATG LDFTNLLGA AQDEDEEKK KEEELIRQG SIRSIASVE GEAPKIVEE QKGTGSVGA DVYLGYFKA GGNCCVIFV LFALFIVTQ IFASIADYF ITYWVNIEQ QDAQKNKTS VAEAQDDDF WHFSRDTSI YIYSVIIGL LIIITLIRS FTFFSVCMR ASTRLHDNM FASITRATM RFFNTNSAG RILNRFSKD MGSIDELLT SAMIDCLQI GLSLLGIII VVAVVSPWL MVPTVVAGI IFYFLRIFY IRTSRNVKR LEGITRSPV FSHLNASLQ GLTTIRAFG AQEILEKEF DGHQDLHSS AWFSFISTS RAFGYWLDV VCIIYITLV TFSFLVIGN EKFGGNVGL AITQAIGLT GMFQWGMRQ STELENQMT SVERVLEYN NIEHEGNLE SPPDKKPAP SWPNDGKIE FINVFLRYF PDDPPVLKN LSFTINPRE KIGIVGRTG AGKSSLINA IFQLSDTQG AIIIDGIDI TEIGLHDLR SKISIIPQE PVLFSGTMR KNLDPFDDY SDADLWRAL EDVELKDEV SNLTSGLNS KMSEGGSNF SVGQRQLVC LARAILRNN KILVLDEAT ANIDPQTDA LIQNTIRNK FSDCTVLTI AHRLHTVMD SDKILVMDA GTMKEFDHA YNLLQDSNT ILYGMVQQT GKAMAETLF NVAKESYNK IHQG
- >TC010434 RC
MLGVMHLGM KMRVACCSL IYRKTLKLS KTALGQTTA GQVVNLLSN DVNRFDVAL LFAHQLWVG PLETIVCTY FMYLQVGYS AIIGVAFLL LFIPLQIFL GKRISVLRL RTALRTDER VRLMNEIIS GIQVIKMYA WEKPFASLV ALARRYEIK SIRISSYMR GITLSFIMF TTRMSIFAS VLAYVLFDN TITAEKVFV LTSFYNILR QTMTVFFPQ GISQVAEAR VSIARLNKF MLYDETQIA KELKRRQAE GKKDNLISN GIDAARDLG VFMKNASAK WSEASSDNT LNNVNLTAV PGKLVAVIG PVGSGKSSL FHAILQELP LFDGSLSVN GEISYASQE PWLFAGSVR QNILFGLPM DKLRYKTVV KKCALERDF TLLPYGDKT MVGDRGVSL SGGQRARIN LARAVYKQA DIYLLDDPL SAVDTHVGK QLFENCIAG YLKNKTVIL ITHQLQYLK EVDQIIYLH DGVVKAQGS FKELQATGL DFTNLLGAA QDEDEEKKK EEELIRQGS IRSIASVEG EAPKIVEEQ KGTGSVGAD VYLGYFKAG GNCCVIFVL FALFIVTQI FASIADYFI TYWVNIEQQ DAQKNKTSV AEAQDDDFW HFSRDTSIY IYSVIIGLL IIITLIRSF TFFSVCMRA STRLHDNMF ASITRATMR FFNTNSAGR ILNRFSKDM GSIDELLTS AMIDCLQIG LSLLGIIIV VAVVSPWLM VPTVVAGII FYFLRIFYI RTSRNVKRL EGITRSPVF SHLNASLQG LTTIRAFGA QEILEKEFD GHQDLHSSA WFSFISTSR AFGYWLDVV CIIYITLVT FSFLVIGNE KFGGNVGLA ITQAIGLTG MFQWGMRQS TELENQMTS VERVLEYNN IEHEGNLES PPDKKPAPS WPNDGKIEF INVFLRYFP DDPPVLKNL SFTINPREK IGIVGRTGA GKSSLINAI FQLSDTQGA IIIDGIDIT EIGLHDLRS KISIIPQEP VLFSGTMRK NLDPFDDYS DADLWRALE DVELKDEVS NLTSGLNSK MSEGGSNFS VGQRQLVCL ARAILRNNK ILVLDEATA NIDPQTDAL IQNTIRNKF SDCTVLTIA HRLHTVMDS DKILVMDAG TMKEFDHAY NLLQDSNTI LYGMVQQTG KAMAETLFN VAKESYNKI HQG
- >TC010434 RA
atggactcg gctcttaaa caaagcaga aagccaaat cctagagaa aatgcattc cctttatca gctgcgacc ttctggtat acgattcca actttcgtt cagggcttc aagcgtgac ttagaagag tccgacttg accgagact ctgactgag cacaagtca agtatactg ggaaataaa atggaaaaa gcttggaaa gctgaagag atcaaagcc gcgaaggct aaccgtaaa ccgtcgcta gaaagagta ctctttaaa gtttttagt tttgaattt gttttttat ggtattgtt ttagcactg agtgaagct ataagattg aaccagcca ctatttttg ggcaagttt gttgcattc tttgatact tatcgaacg ccggttatg cgagatgcc gcccacgtt gtcactgat ggaaccgca caaaatcat tctgaggaa attgggcaa ccgctggct ttgggaaaa ctcctaacg ttctaccaa cccctcaat actgaagtt tcacaaacc gatgcttac tggtacgcc gccggagtc gtgatatgt tccctggca aacattgca ttttcgcac cctcaaatg ttgggggtg atgcattta gggatgaaa atgagggtt gcgtgttgt tcactaatc taccgcaaa acactgaaa ctgagcaaa acggctttg ggtcaaaca actgcgggt caagtggtc aatttgtta tcaaacgat gttaacaga ttcgatgtt gctctactt ttcgcccat cagttatgg gtggggcca cttgagacc atcgtttgt acttacttc atgtactta caagttgga tactcggcg attattggc gttgcgttt cttctgctg tttataccg ctgcaaatt ttcctgggg aagcggatt tcggtcctc cgcctgaga accgcgctc cgaaccgac gaacgcgtc cgtttgatg aacgaaatc atctccggc atccaagtg atcaaaatg tacgcctgg gagaaaccc ttcgcaagt ctggtggct ctcgcccgt cgctatgaa atcaaatcg atccgaatc tcctcctac atgcggggc atcaccctc tccttcatc atgttcacc acccggatg tcgatcttc gcaagtgtc ttagcctac gtcctgttc gacaacacc ataaccgcc gaaaaagtc ttcgtcttg acctcgttc tacaacatt ctgcgccaa accatgacc gtgtttttc ccccaaggc atttcccaa gtggccgag gcgcgcgtc tccatcgcc cggctgaac aaattcatg ctctacgac gagacccag atcgccaaa gaactgaaa cggcgccaa gccgagggc aagaaggac aatctgatc tcgaacggg attgacgcg gcccgcgat ttgggcgtt ttcatgaaa aatgcctca gccaagtgg agcgaagcc tcgagcgat aacacgctc aacaacgtg aatttgacg gcggttccg gggaagttg gtggcggtt attgggccg gttgggagc ggcaagagc agcctgttc catgccatt ttgcaagag ctgcccttg tttgacggg agtctctcg gttaatggg gaaatcagt tacgcgtca caagagccg tggcttttt gcgggaagt gtgcgccaa aacatactc ttcggcctg ccaatggac aagctcagg tacaaaact gtggtgaaa aaatgtgcc ttagaacgc gacttcacc cttttgcct tatggggac aagacgatg gttggggac agaggggtg tccctcagt gggggccag agggcgagg atcaacctc gcgagggcc gtctataaa caagccgat atttaccta ctggatgat ccattatca gctgttgat acacacgtg ggtaaacaa ttgttcgag aattgcatc gccggttac ttgaaaaat aagaccgtc atattgatt actcatcag ttacaatat ttgaaagaa gtggatcaa attatttac ctgcatgac ggggttgtt aaagcacaa gggagtttc aaggaattg caagcgacg gggctggac tttaccaac cttctgggg gcggcgcag gacgaagac gaggagaag aaaaaagaa gaagagttg ataaggcag ggttcgatc cgaagcatt gcctcagtc gaaggggaa gccccgaaa atcgtggag gaacagaag ggcacaggc tcggttggg gccgatgtc tacttgggc tacttcaaa gctgggggc aactgttgt gtcattttc gtacttttc gcactgttt atcgtaact cagattttt gcaagtatt gccgactat tttatcacc tattgggtc aatatcgaa caacaagat gctcaaaaa aacaaaact agcgttgcc gaggcccaa gatgacgat ttctggcac ttctctcgg gacacgtca atttatatt tattcggtc attatcggg cttttgata atcataact ttgatccgt tcgttcacg tttttctcc gtgtgcatg agggcgtcc acccgactt cacgacaac atgtttgcg agcataaca cgggccact atgagattt ttcaatacg aatagtgct ggacggatt ttgaaccgg ttttccaaa gatatgggt tctattgat gaactttta acgtctgcg atgattgat tgcttgcaa atcgggctt tccctcctt ggaattatt atagttgtg gctgttgtt agtccctgg ttgatggta ccgactgtt gttgccgga attattttc tatttcttg aggattttc tacatacgg acaagtcgg aatgtcaaa cgtttggag ggcataacc cgaagtcct gtattttca cacttaaat gcctcgctt caaggctta accacaatt cgggctttt ggtgctcaa gaaatctta gaaaaggag ttcgatggt catcaagat ttgcacagt tcggcttgg tttagcttt atttcaacc tcaagagca ttcgggtat tggctggac gttgtctgc atcatctac atcaccttg gtcacgttt agtttcttg gttattgga aacgaaaaa tttggtggc aacgtgggg ctggccata acgcaggcc atcggcctg accggcatg ttccagtgg ggcatgcgt cagtccacg gagctggag aaccagatg acctccgtg gagcgcgtc ctcgagtac aacaacatc gagcacgag ggcaacctg gagtcgccc ccggacaaa aagccggct ccgtcgtgg cccaacgac ggcaaaatc gagttcatc aacgtgttc ctgcgctat ttccccgac gacccgccc gtgctcaag aacctaagt ttcacgata aacccgcgg gagaagatc gggattgtg ggccgcaca ggcgccggc aagtcctcg ctaatcaac gcgattttc caactcagc gacacgcag ggcgccatc atcatcgac ggcatcgac atcaccgag atcggtctg cacgacttg cgctcaaaa atctcgata atcccgcag gagccggtg ctgttctcc gggactatg cgcaagaac ttggacccg ttcgacgac tactcggac gccgacctg tggcgcgct ttggaggac gtggagttg aaggacgag gtgtcgaac ttgacctcg ggactcaac tcgaaaatg tcggaggga gggtcgaat ttcagtgtg gggcagagg caactggtg tgtctggcg cgggcgatt ttgcgcaac aataagatt cttgttctg gacgaagcc acggctaat attgacccg caaaccgat gcgttgatt cagaacacc attaggaac aaattctcg gattgtacg gtacttacg atcgctcat aggctgcac actgtgatg gattcggac aagattctg gtcatggat gcggggaca atgaaggag tttgaccat gcgtataat ctgttgcag gatagcaat actatactg tacggcatg gtccagcaa actggcaag gccatggcc gaaacattg ttcaatgtt gctaaagaa agctacaac aaaatacat caaggttag
- >TC010434 RB
atggactcg gctcttaaa caaagcaga aagccaaat cctagagaa aatgcattc cctttatca gctgcgacc ttctggtat acgattcca actttcgtt cagggcttc aagcgtgac ttagaagag tccgacttg accgagact ctgactgag cacaagtca agtatactg ggaaataaa atggaaaaa gcttggaaa gctgaagag atcaaagcc gcgaaggct aaccgtaaa ccgtcgcta gaaagagta ctctttaaa gtttttagt tttgaattt gttttttat ggtattgtt ttagcactg agtgaagct ataagaatt gggcaaccg ctggctttg ggaaaactc ctaacgttc taccaaccc ctcaatact gaagtttca caaaccgat gcttactgg tacgccgcc ggagtcgtg atatgttcc ctggcaaac attgcattt tcgcaccct caaatgttg ggggtgatg catttaggg atgaaaatg agggttgcg tgttgttca ctaatctac cgcaaaaca ctgaaactg agcaaaacg gctttgggt caaacaact gcgggtcaa gtggtcaat ttgttatca aacgatgtt aacagattc gatgttgct ctacttttc gcccatcag ttatgggtg gggccactt gagaccatc gtttgtact tacttcatg tacttacaa gttggatac tcggcgatt attggcgtt gcgtttctt ctgctgttt ataccgctg caaattttc ctggggaag cggatttcg gtcctccgc ctgagaacc gcgctccga accgacgaa cgcgtccgt ttgatgaac gaaatcatc tccggcatc caagtgatc aaaatgtac gcctgggag aaacccttc gcaagtctg gtggctctc gcccgtcgc tatgaaatc aaatcgatc cgaatctcc tcctacatg cggggcatc accctctcc ttcatcatg ttcaccacc cggatgtcg atcttcgca agtgtctta gcctacgtc ctgttcgac aacaccata accgccgaa aaagtcttc gtcttgacc tcgttctac aacattctg cgccaaacc atgaccgtg tttttcccc caaggcatt tcccaagtg gccgaggcg cgcgtctcc atcgcccgg ctgaacaaa ttcatgctc tacgacgag acccagatc gccaaagaa ctgaaacgg cgccaagcc gagggcaag aaggacaat ctgatctcg aacgggatt gacgcggcc cgcgatttg ggcgttttc atgaaaaat gcctcagcc aagtggagc gaagcctcg agcgataac acgctcaac aacgtgaat ttgacggcg gttccgggg aagttggtg gcggttatt gggccggtt gggagcggc aagagcagc ctgttccat gccattttg caagagctg cccttgttt gacgggagt ctctcggtt aatggggaa atcagttac gcgtcacaa gagccgtgg ctttttgcg ggaagtgtg cgccaaaac atactcttc ggcctgcca atggacaag ctcaggtac aaaactgtg gtgaaaaaa tgtgcctta gaacgcgac ttcaccctt ttgccttat ggggacaag acgatggtt ggggacaga ggggtgtcc ctcagtggg ggccagagg gcgaggatc aacctcgcg agggccgtc tataaacaa gccgatatt tacctactg gatgatcca ttatcagct gttgataca cacgtgggt aaacaattg ttcgagaat tgcatcgcc ggttacttg aaaaataag accgtcata ttgattact catcagtta caatatttg aaagaagtg gatcaaatt atttacctg catgacggg gttgttaaa gcacaaggg agtttcaag gaattgcaa gcgacgggg ctggacttt accaacctt ctgggggcg gcgcaggac gaagacgag gagaagaaa aaagaagaa gagttgata aggcagggt tcgatccga agcattgcc tcagtcgaa ggggaagcc ccgaaaatc gtggaggaa cagaagggc acaggctcg gttggggcc gatgtctac ttgggctac ttcaaagct gggggcaac tgttgtgtc attttcgta cttttcgca ctgtttatc gtaactcag atttttgca agtattgcc gactatttt atcacctat tgggtcaat atcgaacaa caagatgct caaaaaaac aaaactagc gttgccgag gcccaagat gacgatttc tggcacttc tctcgggac acgtcaatt tatatttat tcggtcatt atcgggctt ttgataatc ataactttg atccgttcg ttcacgttt ttctccgtg tgcatgagg gcgtccacc cgacttcac gacaacatg tttgcgagc ataacacgg gccactatg agatttttc aatacgaat agtgctgga cggattttg aaccggttt tccaaagat atgggttct attgatgaa cttttaacg tctgcgatg attgattgc ttgcaaatc gggctttcc ctccttgga attattata gttgtggct gttgttagt ccctggttg atggtaccg actgttgtt gccggaatt attttctat ttcttgagg attttctac atacggaca agtcggaat gtcaaacgt ttggagggc ataacccga agtcctgta ttttcacac ttaaatgcc tcgcttcaa ggcttaacc acaattcgg gcttttggt gctcaagaa atcttagaa aaggagttc gatggtcat caagatttg cacagttcg gcttggttt agctttatt tcaacctca agagcattc gggtattgg ctggacgtt gtctgcatc atctacatc accttggtc acgtttagt ttcttggtt attggaaac gaaaaattt ggtggcaac gtggggctg gccataacg caggccatc ggcctgacc ggcatgttc cagtggggc atgcgtcag tccacggag ctggagaac cagatgacc tccgtggag cgcgtcctc gagtacaac aacatcgag cacgagggc aacctggag tcgcccccg gacaaaaag ccggctccg tcgtggccc aacgacggc aaaatcgag ttcatcaac gtgttcctg cgctatttc cccgacgac ccgcccgtg ctcaagaac ctaagtttc acgataaac ccgcgggag aagatcggg attgtgggc cgcacaggc gccggcaag tcctcgcta atcaacgcg attttccaa ctcagcgac acgcagggc gccatcatc atcgacggc atcgacatc accgagatc ggtctgcac gacttgcgc tcaaaaatc tcgataatc ccgcaggag ccggtgctg ttctccggg actatgcgc aagaacttg gacccgttc gacgactac tcggacgcc gacctgtgg cgcgctttg gaggacgtg gagttgaag gacgaggtg tcgaacttg acctcggga ctcaactcg aaaatgtcg gagggaggg tcgaatttc agtgtgggg cagaggcaa ctggtgtgt ctggcgcgg gcgattttg cgcaacaat aagattctt gttctggac gaagccacg gctaatatt gacccgcaa accgatgcg ttgattcag aacaccatt aggaacaaa ttctcggat tgtacggta cttacgatc gctcatagg ctgcacact gtgatggat tcggacaag attctggtc atggatgcg gggacaatg aaggagttt gaccatgcg tataatctg ttgcaggat agcaatact atactgtac ggcatggtc cagcaaact ggcaaggcc atggccgaa acattgttc aatgttgct aaagaaagc tacaacaaa atacatcaa ggttag
- >TC010434 RC
atgttgggg gtgatgcat ttagggatg aaaatgagg gttgcgtgt tgttcacta atctaccgc aaaacactg aaactgagc aaaacggct ttgggtcaa acaactgcg ggtcaagtg gtcaatttg ttatcaaac gatgttaac agattcgat gttgctcta cttttcgcc catcagtta tgggtgggg ccacttgag accatcgtt tgtacttac ttcatgtac ttacaagtt ggatactcg gcgattatt ggcgttgcg tttcttctg ctgtttata ccgctgcaa attttcctg gggaagcgg atttcggtc ctccgcctg agaaccgcg ctccgaacc gacgaacgc gtccgtttg atgaacgaa atcatctcc ggcatccaa gtgatcaaa atgtacgcc tgggagaaa cccttcgca agtctggtg gctctcgcc cgtcgctat gaaatcaaa tcgatccga atctcctcc tacatgcgg ggcatcacc ctctccttc atcatgttc accacccgg atgtcgatc ttcgcaagt gtcttagcc tacgtcctg ttcgacaac accataacc gccgaaaaa gtcttcgtc ttgacctcg ttctacaac attctgcgc caaaccatg accgtgttt ttcccccaa ggcatttcc caagtggcc gaggcgcgc gtctccatc gcccggctg aacaaattc atgctctac gacgagacc cagatcgcc aaagaactg aaacggcgc caagccgag ggcaagaag gacaatctg atctcgaac gggattgac gcggcccgc gatttgggc gttttcatg aaaaatgcc tcagccaag tggagcgaa gcctcgagc gataacacg ctcaacaac gtgaatttg acggcggtt ccggggaag ttggtggcg gttattggg ccggttggg agcggcaag agcagcctg ttccatgcc attttgcaa gagctgccc ttgtttgac gggagtctc tcggttaat ggggaaatc agttacgcg tcacaagag ccgtggctt tttgcggga agtgtgcgc caaaacata ctcttcggc ctgccaatg gacaagctc aggtacaaa actgtggtg aaaaaatgt gccttagaa cgcgacttc acccttttg ccttatggg gacaagacg atggttggg gacagaggg gtgtccctc agtgggggc cagagggcg aggatcaac ctcgcgagg gccgtctat aaacaagcc gatatttac ctactggat gatccatta tcagctgtt gatacacac gtgggtaaa caattgttc gagaattgc atcgccggt tacttgaaa aataagacc gtcatattg attactcat cagttacaa tatttgaaa gaagtggat caaattatt tacctgcat gacggggtt gttaaagca caagggagt ttcaaggaa ttgcaagcg acggggctg gactttacc aaccttctg ggggcggcg caggacgaa gacgaggag aagaaaaaa gaagaagag ttgataagg cagggttcg atccgaagc attgcctca gtcgaaggg gaagccccg aaaatcgtg gaggaacag aagggcaca ggctcggtt ggggccgat gtctacttg ggctacttc aaagctggg ggcaactgt tgtgtcatt ttcgtactt ttcgcactg tttatcgta actcagatt tttgcaagt attgccgac tattttatc acctattgg gtcaatatc gaacaacaa gatgctcaa aaaaacaaa actagcgtt gccgaggcc caagatgac gatttctgg cacttctct cgggacacg tcaatttat atttattcg gtcattatc gggcttttg ataatcata actttgatc cgttcgttc acgtttttc tccgtgtgc atgagggcg tccacccga cttcacgac aacatgttt gcgagcata acacgggcc actatgaga tttttcaat acgaatagt gctggacgg attttgaac cggttttcc aaagatatg ggttctatt gatgaactt ttaacgtct gcgatgatt gattgcttg caaatcggg ctttccctc cttggaatt attatagtt gtggctgtt gttagtccc tggttgatg gtaccgact gttgttgcc ggaattatt ttctatttc ttgaggatt ttctacata cggacaagt cggaatgtc aaacgtttg gagggcata acccgaagt cctgtattt tcacactta aatgcctcg cttcaaggc ttaaccaca attcgggct tttggtgct caagaaatc ttagaaaag gagttcgat ggtcatcaa gatttgcac agttcggct tggtttagc tttatttca acctcaaga gcattcggg tattggctg gacgttgtc tgcatcatc tacatcacc ttggtcacg tttagtttc ttggttatt ggaaacgaa aaatttggt ggcaacgtg gggctggcc ataacgcag gccatcggc ctgaccggc atgttccag tggggcatg cgtcagtcc acggagctg gagaaccag atgacctcc gtggagcgc gtcctcgag tacaacaac atcgagcac gagggcaac ctggagtcg cccccggac aaaaagccg gctccgtcg tggcccaac gacggcaaa atcgagttc atcaacgtg ttcctgcgc tatttcccc gacgacccg cccgtgctc aagaaccta agtttcacg ataaacccg cgggagaag atcgggatt gtgggccgc acaggcgcc ggcaagtcc tcgctaatc aacgcgatt ttccaactc agcgacacg cagggcgcc atcatcatc gacggcatc gacatcacc gagatcggt ctgcacgac ttgcgctca aaaatctcg ataatcccg caggagccg gtgctgttc tccgggact atgcgcaag aacttggac ccgttcgac gactactcg gacgccgac ctgtggcgc gctttggag gacgtggag ttgaaggac gaggtgtcg aacttgacc tcgggactc aactcgaaa atgtcggag ggagggtcg aatttcagt gtggggcag aggcaactg gtgtgtctg gcgcgggcg attttgcgc aacaataag attcttgtt ctggacgaa gccacggct aatattgac ccgcaaacc gatgcgttg attcagaac accattagg aacaaattc tcggattgt acggtactt acgatcgct cataggctg cacactgtg atggattcg gacaagatt ctggtcatg gatgcgggg acaatgaag gagtttgac catgcgtat aatctgttg caggatagc aatactata ctgtacggc atggtccag caaactggc aaggccatg gccgaaaca ttgttcaat gttgctaaa gaaagctac aacaaaata catcaaggt tag
- >TC010434 RA
catccttga attttagtg cgggaacta aattaaact cgtgccaag acactgaat gggtttctc cgattcatc aaatcaaaa tctttaatt ttcttacgt ttctgcatg aggcagggg ggtttttcg gtactcgtt tgaacaggt tatggactc ggctcttaa acaaagcag aaagccaaa tcctagaga aaatgcatt ccctttatc agctgcgac cttctggta tacgattcc aactttcgt tcagggctt caagcgtga cttagaaga gtccgactt gaccgagac tctgactga gcacaagtc aagtatact gggaaataa aatggaaaa agcttggaa agctgaaga gatcaaagc cgcgaaggc taaccgtaa accgtcgct agaaagagt actctttaa agtttttag ttttgaatt tgtttttta tggtattgt tttagcact gagtgaagc tataagatt gaaccagcc actattttt gggcaagtt tgttgcatt ctttgatac ttatcgaac gccggttat gcgagatgc cgcccacgt tgtcactga tggaaccgc acaaaatca ttctgagga aattgggca accgctggc tttgggaaa actcctaac gttctacca acccctcaa tactgaagt ttcacaaac cgatgctta ctggtacgc cgccggagt cgtgatatg ttccctggc aaacattgc attttcgca ccctcaaat gttgggggt gatgcattt agggatgaa aatgagggt tgcgtgttg ttcactaat ctaccgcaa aacactgaa actgagcaa aacggcttt gggtcaaac aactgcggg tcaagtggt caatttgtt atcaaacga tgttaacag attcgatgt tgctctact tttcgccca tcagttatg ggtggggcc acttgagac catcgtttg tacttactt catgtactt acaagttgg atactcggc gattattgg cgttgcgtt tcttctgct gtttatacc gctgcaaat tttcctggg gaagcggat ttcggtcct ccgcctgag aaccgcgct ccgaaccga cgaacgcgt ccgtttgat gaacgaaat catctccgg catccaagt gatcaaaat gtacgcctg ggagaaacc cttcgcaag tctggtggc tctcgcccg tcgctatga aatcaaatc gatccgaat ctcctccta catgcgggg catcaccct ctccttcat catgttcac cacccggat gtcgatctt cgcaagtgt cttagccta cgtcctgtt cgacaacac cataaccgc cgaaaaagt cttcgtctt gacctcgtt ctacaacat tctgcgcca aaccatgac cgtgttttt cccccaagg catttccca agtggccga ggcgcgcgt ctccatcgc ccggctgaa caaattcat gctctacga cgagaccca gatcgccaa agaactgaa acggcgcca agccgaggg caagaagga caatctgat ctcgaacgg gattgacgc ggcccgcga tttgggcgt tttcatgaa aaatgcctc agccaagtg gagcgaagc ctcgagcga taacacgct caacaacgt gaatttgac ggcggttcc ggggaagtt ggtggcggt tattgggcc ggttgggag cggcaagag cagcctgtt ccatgccat tttgcaaga gctgccctt gtttgacgg gagtctctc ggttaatgg ggaaatcag ttacgcgtc acaagagcc gtggctttt tgcgggaag tgtgcgcca aaacatact cttcggcct gccaatgga caagctcag gtacaaaac tgtggtgaa aaaatgtgc cttagaacg cgacttcac ccttttgcc ttatgggga caagacgat ggttgggga cagaggggt gtccctcag tgggggcca gagggcgag gatcaacct cgcgagggc cgtctataa acaagccga tatttacct actggatga tccattatc agctgttga tacacacgt gggtaaaca attgttcga gaattgcat cgccggtta cttgaaaaa taagaccgt catattgat tactcatca gttacaata tttgaaaga agtggatca aattattta cctgcatga cggggttgt taaagcaca agggagttt caaggaatt gcaagcgac ggggctgga ctttaccaa ccttctggg ggcggcgca ggacgaaga cgaggagaa gaaaaaaga agaagagtt gataaggca gggttcgat ccgaagcat tgcctcagt cgaagggga agccccgaa aatcgtgga ggaacagaa gggcacagg ctcggttgg ggccgatgt ctacttggg ctacttcaa agctggggg caactgttg tgtcatttt cgtactttt cgcactgtt tatcgtaac tcagatttt tgcaagtat tgccgacta ttttatcac ctattgggt caatatcga acaacaaga tgctcaaaa aaacaaaac tagcgttgc cgaggccca agatgacga tttctggca cttctctcg ggacacgtc aatttatat ttattcggt cattatcgg gcttttgat aatcataac tttgatccg ttcgttcac gtttttctc cgtgtgcat gagggcgtc cacccgact tcacgacaa catgtttgc gagcataac acgggccac tatgagatt tttcaatac gaatagtgc tggacggat tttgaaccg gttttccaa agatatggg ttctattga tgaactttt aacgtctgc gatgattga ttgcttgca aatcgggct ttccctcct tggaattat tatagttgt ggctgttgt tagtccctg gttgatggt accgactgt tgttgccgg aattatttt ctatttctt gaggatttt ctacatacg gacaagtcg gaatgtcaa acgtttgga gggcataac ccgaagtcc tgtattttc acacttaaa tgcctcgct tcaaggctt aaccacaat tcgggcttt tggtgctca agaaatctt agaaaagga gttcgatgg tcatcaaga tttgcacag ttcggcttg gtttagctt tatttcaac ctcaagagc attcgggta ttggctgga cgttgtctg catcatcta catcacctt ggtcacgtt tagtttctt ggttattgg aaacgaaaa atttggtgg caacgtggg gctggccat aacgcaggc catcggcct gaccggcat gttccagtg gggcatgcg tcagtccac ggagctgga gaaccagat gacctccgt ggagcgcgt cctcgagta caacaacat cgagcacga gggcaacct ggagtcgcc cccggacaa aaagccggc tccgtcgtg gcccaacga cggcaaaat cgagttcat caacgtgtt cctgcgcta tttccccga cgacccgcc cgtgctcaa gaacctaag tttcacgat aaacccgcg ggagaagat cgggattgt gggccgcac aggcgccgg caagtcctc gctaatcaa cgcgatttt ccaactcag cgacacgca gggcgccat catcatcga cggcatcga catcaccga gatcggtct gcacgactt gcgctcaaa aatctcgat aatcccgca ggagccggt gctgttctc cgggactat gcgcaagaa cttggaccc gttcgacga ctactcgga cgccgacct gtggcgcgc tttggagga cgtggagtt gaaggacga ggtgtcgaa cttgacctc gggactcaa ctcgaaaat gtcggaggg agggtcgaa tttcagtgt ggggcagag gcaactggt gtgtctggc gcgggcgat tttgcgcaa caataagat tcttgttct ggacgaagc cacggctaa tattgaccc gcaaaccga tgcgttgat tcagaacac cattaggaa caaattctc ggattgtac ggtacttac gatcgctca taggctgca cactgtgat ggattcgga caagattct ggtcatgga tgcggggac aatgaagga gtttgacca tgcgtataa tctgttgca ggatagcaa tactatact gtacggcat ggtccagca aactggcaa ggccatggc cgaaacatt gttcaatgt tgctaaaga aagctacaa caaaataca tcaaggtta gtccaattc taggttgca aatttggga cagtactta agggctggc tcctgtttt aacgaagat tctgcgata atagtttta atttggaga acaaatttt atatttta
- >TC010434 RB
catccttga attttagtg cgggaacta aattaaact cgtgccaag acactgaat gggtttctc cgattcatc aaatcaaaa tctttaatt ttcttacgt ttctgcatg aggcagggg ggtttttcg gtactcgtt tgaacaggt tatggactc ggctcttaa acaaagcag aaagccaaa tcctagaga aaatgcatt ccctttatc agctgcgac cttctggta tacgattcc aactttcgt tcagggctt caagcgtga cttagaaga gtccgactt gaccgagac tctgactga gcacaagtc aagtatact gggaaataa aatggaaaa agcttggaa agctgaaga gatcaaagc cgcgaaggc taaccgtaa accgtcgct agaaagagt actctttaa agtttttag ttttgaatt tgtttttta tggtattgt tttagcact gagtgaagc tataagaat tgggcaacc gctggcttt gggaaaact cctaacgtt ctaccaacc cctcaatac tgaagtttc acaaaccga tgcttactg gtacgccgc cggagtcgt gatatgttc cctggcaaa cattgcatt ttcgcaccc tcaaatgtt gggggtgat gcatttagg gatgaaaat gagggttgc gtgttgttc actaatcta ccgcaaaac actgaaact gagcaaaac ggctttggg tcaaacaac tgcgggtca agtggtcaa tttgttatc aaacgatgt taacagatt cgatgttgc tctactttt cgcccatca gttatgggt ggggccact tgagaccat cgtttgtac ttacttcat gtacttaca agttggata ctcggcgat tattggcgt tgcgtttct tctgctgtt tataccgct gcaaatttt cctggggaa gcggatttc ggtcctccg cctgagaac cgcgctccg aaccgacga acgcgtccg tttgatgaa cgaaatcat ctccggcat ccaagtgat caaaatgta cgcctggga gaaaccctt cgcaagtct ggtggctct cgcccgtcg ctatgaaat caaatcgat ccgaatctc ctcctacat gcggggcat caccctctc cttcatcat gttcaccac ccggatgtc gatcttcgc aagtgtctt agcctacgt cctgttcga caacaccat aaccgccga aaaagtctt cgtcttgac ctcgttcta caacattct gcgccaaac catgaccgt gtttttccc ccaaggcat ttcccaagt ggccgaggc gcgcgtctc catcgcccg gctgaacaa attcatgct ctacgacga gacccagat cgccaaaga actgaaacg gcgccaagc cgagggcaa gaaggacaa tctgatctc gaacgggat tgacgcggc ccgcgattt gggcgtttt catgaaaaa tgcctcagc caagtggag cgaagcctc gagcgataa cacgctcaa caacgtgaa tttgacggc ggttccggg gaagttggt ggcggttat tgggccggt tgggagcgg caagagcag cctgttcca tgccatttt gcaagagct gcccttgtt tgacgggag tctctcggt taatgggga aatcagtta cgcgtcaca agagccgtg gctttttgc gggaagtgt gcgccaaaa catactctt cggcctgcc aatggacaa gctcaggta caaaactgt ggtgaaaaa atgtgcctt agaacgcga cttcaccct tttgcctta tggggacaa gacgatggt tggggacag aggggtgtc cctcagtgg gggccagag ggcgaggat caacctcgc gagggccgt ctataaaca agccgatat ttacctact ggatgatcc attatcagc tgttgatac acacgtggg taaacaatt gttcgagaa ttgcatcgc cggttactt gaaaaataa gaccgtcat attgattac tcatcagtt acaatattt gaaagaagt ggatcaaat tatttacct gcatgacgg ggttgttaa agcacaagg gagtttcaa ggaattgca agcgacggg gctggactt taccaacct tctgggggc ggcgcagga cgaagacga ggagaagaa aaaagaaga agagttgat aaggcaggg ttcgatccg aagcattgc ctcagtcga aggggaagc cccgaaaat cgtggagga acagaaggg cacaggctc ggttggggc cgatgtcta cttgggcta cttcaaagc tgggggcaa ctgttgtgt cattttcgt acttttcgc actgtttat cgtaactca gatttttgc aagtattgc cgactattt tatcaccta ttgggtcaa tatcgaaca acaagatgc tcaaaaaaa caaaactag cgttgccga ggcccaaga tgacgattt ctggcactt ctctcggga cacgtcaat ttatattta ttcggtcat tatcgggct tttgataat cataacttt gatccgttc gttcacgtt tttctccgt gtgcatgag ggcgtccac ccgacttca cgacaacat gtttgcgag cataacacg ggccactat gagattttt caatacgaa tagtgctgg acggatttt gaaccggtt ttccaaaga tatgggttc tattgatga acttttaac gtctgcgat gattgattg cttgcaaat cgggctttc cctccttgg aattattat agttgtggc tgttgttag tccctggtt gatggtacc gactgttgt tgccggaat tattttcta tttcttgag gattttcta catacggac aagtcggaa tgtcaaacg tttggaggg cataacccg aagtcctgt attttcaca cttaaatgc ctcgcttca aggcttaac cacaattcg ggcttttgg tgctcaaga aatcttaga aaaggagtt cgatggtca tcaagattt gcacagttc ggcttggtt tagctttat ttcaacctc aagagcatt cgggtattg gctggacgt tgtctgcat catctacat caccttggt cacgtttag tttcttggt tattggaaa cgaaaaatt tggtggcaa cgtggggct ggccataac gcaggccat cggcctgac cggcatgtt ccagtgggg catgcgtca gtccacgga gctggagaa ccagatgac ctccgtgga gcgcgtcct cgagtacaa caacatcga gcacgaggg caacctgga gtcgccccc ggacaaaaa gccggctcc gtcgtggcc caacgacgg caaaatcga gttcatcaa cgtgttcct gcgctattt ccccgacga cccgcccgt gctcaagaa cctaagttt cacgataaa cccgcggga gaagatcgg gattgtggg ccgcacagg cgccggcaa gtcctcgct aatcaacgc gattttcca actcagcga cacgcaggg cgccatcat catcgacgg catcgacat caccgagat cggtctgca cgacttgcg ctcaaaaat ctcgataat cccgcagga gccggtgct gttctccgg gactatgcg caagaactt ggacccgtt cgacgacta ctcggacgc cgacctgtg gcgcgcttt ggaggacgt ggagttgaa ggacgaggt gtcgaactt gacctcggg actcaactc gaaaatgtc ggagggagg gtcgaattt cagtgtggg gcagaggca actggtgtg tctggcgcg ggcgatttt gcgcaacaa taagattct tgttctgga cgaagccac ggctaatat tgacccgca aaccgatgc gttgattca gaacaccat taggaacaa attctcgga ttgtacggt acttacgat cgctcatag gctgcacac tgtgatgga ttcggacaa gattctggt catggatgc ggggacaat gaaggagtt tgaccatgc gtataatct gttgcagga tagcaatac tatactgta cggcatggt ccagcaaac tggcaaggc catggccga aacattgtt caatgttgc taaagaaag ctacaacaa aatacatca aggttagtc caattctag gttgcaaat ttgggacag tacttaagg gctggctcc tgttttaac gaagattct gcgataata gttttaatt tggagaaca aattttata tttta
- >TC010434 RC
tcagtattg tttgatact tattggtga atagttgaa aagtgaaac acttttctc gagcttaag tagggtaat agaattttt actcaaatc gtactaacc tcccccgtt gtttcagac gtttctgca tgaggcagg ggggttttt cggtactcg tttgaacag gttatggac tcggctctt aaacaaagc agaaagcca aatcctaga gaaaatgca ttcccttta tcagctgcg accttctgg tatacgatt ccaactttc gttcagggc ttcaagcgt gacttagaa gagtccgac ttgaccgag actctgact gagcacaag tcaagtata ctgggaaat aaaatggaa aaagcttgg aaagctgaa gagatcaaa gccgcgaag gctaaccgt aaaccgtcg ctagaaaga gtactcttt aaagttttt agttttgaa tttgttttt tatggtatt gttttagca ctgagtgaa gctataagg tgagttgag ggtatcatt cactatcat tctatgttc tgttcgact tgtttctct catagtgga taatatgaa ataattttg gtatttaga ttgaaccag ccactattt ttgggcaag tttgttgca ttctttgat acttatcga acgccggtt atgcgagat gccgcccac gttgtcact gatggaacc gcacaaaat cattctgag gagtaaata tttaccgca gtgtgtgac tagagtaga gttgggcct tttctttca aatcttccg ttctatttt ttatttagt ttcattaca ctgtaaata gcctccttc atctgtaca ttagcacaa tttttattt ttattttgc atgctcctt ttactcctc ttttttgca tgttttgtt gtttttagt gttttagca gattcaaaa atgccgttt ttccagaat tgggcaacc gctggcttt gggaaaact cctaacgtt ctaccaacc cctcaatac tgaagtttc acaaaccga tgcttactg gtacgccgc cggagtcgt gatatgttc cctggcaaa cattgcatt ttcgcaccc tcaaatgtt gggggtgat gcatttagg gatgaaaat gagggttgc gtgttgttc actaatcta ccgcaaaac actgaaact gagcaaaac ggctttggg tcaaacaac tgcgggtca agtggtcaa tttgttatc aaacgatgt taacagatt cgatgttgc tctactttt cgcccatca gttatgggt ggggccact tgagaccat cgtttgtac ttacttcat gtacttaca agttggata ctcggcgat tattggcgt tgcgtttct tctgctgtt tataccgct gcaaatttt cctggggaa gcggatttc ggtcctccg cctgagaac cgcgctccg aaccgacga acgcgtccg tttgatgaa cgaaatcat ctccggcat ccaagtgat caaaatgta cgcctggga gaaaccctt cgcaagtct ggtggctct cgcccgtcg ctatgaaat caaatcgat ccgaatctc ctcctacat gcggggcat caccctctc cttcatcat gttcaccac ccggatgtc gatcttcgc aagtgtctt agcctacgt cctgttcga caacaccat aaccgccga aaaagtctt cgtcttgac ctcgttcta caacattct gcgccaaac catgaccgt gtttttccc ccaaggcat ttcccaagt ggccgaggc gcgcgtctc catcgcccg gctgaacaa attcatgct ctacgacga gacccagat cgccaaaga actgaaacg gcgccaagc cgagggcaa gaaggacaa tctgatctc gaacgggat tgacgcggc ccgcgattt gggcgtttt catgaaaaa tgcctcagc caagtggag cgaagcctc gagcgataa cacgctcaa caacgtgaa tttgacggc ggttccggg gaagttggt ggcggttat tgggccggt tgggagcgg caagagcag cctgttcca tgccatttt gcaagagct gcccttgtt tgacgggag tctctcggt taatgggga aatcagtta cgcgtcaca agagccgtg gctttttgc gggaagtgt gcgccaaaa catactctt cggcctgcc aatggacaa gctcaggta caaaactgt ggtgaaaaa atgtgcctt agaacgcga cttcaccct tttgcctta tggggacaa gacgatggt tggggacag aggggtgtc cctcagtgg gggccagag ggcgaggat caacctcgc gagggccgt ctataaaca agccgatat ttacctact ggatgatcc attatcagc tgttgatac acacgtggg taaacaatt gttcgagaa ttgcatcgc cggttactt gaaaaataa gaccgtcat attgattac tcatcagtt acaatattt gaaagaagt ggatcaaat tatttacct gcatgacgg ggttgttaa agcacaagg gagtttcaa ggaattgca agcgacggg gctggactt taccaacct tctgggggc ggcgcagga cgaagacga ggagaagaa aaaagaaga agagttgat aaggcaggg ttcgatccg aagcattgc ctcagtcga aggggaagc cccgaaaat cgtggagga acagaaggg cacaggctc ggttggggc cgatgtcta cttgggcta cttcaaagc tgggggcaa ctgttgtgt cattttcgt acttttcgc actgtttat cgtaactca gatttttgc aagtattgc cgactattt tatcaccta ttgggtcaa tatcgaaca acaagatgc tcaaaaaaa caaaactag cgttgccga ggcccaaga tgacgattt ctggcactt ctctcggga cacgtcaat ttatattta ttcggtcat tatcgggct tttgataat cataacttt gatccgttc gttcacgtt tttctccgt gtgcatgag ggcgtccac ccgacttca cgacaacat gtttgcgag cataacacg ggccactat gagattttt caatacgaa tagtgctgg acggatttt gaaccggtt ttccaaaga tatgggttc tattgatga acttttaac gtctgcgat gattgattg cttgcaaat cgggctttc cctccttgg aattattat agttgtggc tgttgttag tccctggtt gatggtacc gactgttgt tgccggaat tattttcta tttcttgag gattttcta catacggac aagtcggaa tgtcaaacg tttggaggg cataacccg aagtcctgt attttcaca cttaaatgc ctcgcttca aggcttaac cacaattcg ggcttttgg tgctcaaga aatcttaga aaaggagtt cgatggtca tcaagattt gcacagttc ggcttggtt tagctttat ttcaacctc aagagcatt cgggtattg gctggacgt tgtctgcat catctacat caccttggt cacgtttag tttcttggt tattggaaa cgaaaaatt tggtggcaa cgtggggct ggccataac gcaggccat cggcctgac cggcatgtt ccagtgggg catgcgtca gtccacgga gctggagaa ccagatgac ctccgtgga gcgcgtcct cgagtacaa caacatcga gcacgaggg caacctgga gtcgccccc ggacaaaaa gccggctcc gtcgtggcc caacgacgg caaaatcga gttcatcaa cgtgttcct gcgctattt ccccgacga cccgcccgt gctcaagaa cctaagttt cacgataaa cccgcggga gaagatcgg gattgtggg ccgcacagg cgccggcaa gtcctcgct aatcaacgc gattttcca actcagcga cacgcaggg cgccatcat catcgacgg catcgacat caccgagat cggtctgca cgacttgcg ctcaaaaat ctcgataat cccgcagga gccggtgct gttctccgg gactatgcg caagaactt ggacccgtt cgacgacta ctcggacgc cgacctgtg gcgcgcttt ggaggacgt ggagttgaa ggacgaggt gtcgaactt gacctcggg actcaactc gaaaatgtc ggagggagg gtcgaattt cagtgtggg gcagaggca actggtgtg tctggcgcg ggcgatttt gcgcaacaa taagattct tgttctgga cgaagccac ggctaatat tgacccgca aaccgatgc gttgattca gaacaccat taggaacaa attctcgga ttgtacggt acttacgat cgctcatag gctgcacac tgtgatgga ttcggacaa gattctggt catggatgc ggggacaat gaaggagtt tgaccatgc gtataatct gttgcagga tagcaatac tatactgta cggcatggt ccagcaaac tggcaaggc catggccga aacattgtt caatgttgc taaagaaag ctacaacaa aatacatca aggttagtc caattctag gttgcaaat ttgggacag tacttaagg gctggctcc tgttttaac gaagattct gcgataata gttttaatt tggagaaca aattttata tttta
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