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Gene information
General information
TC010433 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 6277878..6281525
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010433
    iBeetle screen iB_01689
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    legs femur broader - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs tibiotarsus broader - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs femur shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax tibiotarsus shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs segment potentially fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010433 RA
      MLGFGKKGA ISNSAEKQE TLLTLSILT LAAILSFAT 
      RLFSVLRFE SVIHEFDPY FNYRTTKFL AEEGFYAFH 
      NWFDDRAWY PLGRIIGGT IYPGLMVTS AAFYHLCWL 
      FNITIDIRN VCVFLAPLF SSFTTIITY LLTKEVKNA 
      GAGLVAAAM VSIVPGYIS RSVAGSYDN EGIAIFCML 
      LTYYTWIKA VKTGTILWG TFAALAYFY MVSSWGGYV 
      FLINLIPLH VITLMVTGR FSHRIYIAY STLYCVGTI 
      LSMQISFVG FQPVQSSEH MLALGVFGL CQIYAFVDY 
      LRSKLTKND FDVLFNFLI YTVGILFIV VFGVLTLSG 
      KISPWTGRF YSLLDPSYA KNHIPIIAS VSEHQPTSW 
      SSFYFDLQI LVFLFPAGL YFCFSQLTD ANIFMILYG 
      VTSIYFAGV MVRLMLVLA PVMCILSGI AVSHLLTKF 
      MKNTDTAKP SEHKKSKKL ESNYVLKNE ISSAFVGVI 
      CLLLISYTF HCTWVTSEA YSSPSIVLS ARSHDGGRI 
      IFDDFREAY YWLKMNTPE DARIMSWWD YGYQITAMA 
      NRTILVDNN TWNNTHISR VGQAMASSE EKAYEIMKE 
      LDVDYVLVI FGGLTGYSS DDINKFLWM VRIGGSTDR 
      GAHIKEWNY YSSSGEFRV DKEGSQTLL NCLMYKMCY 
      YRFGQVYTE GGKPPGYDR VRGAEIGNK DFELDVLEE 
      AYTTEHWLV RIYKVKDLP NRGV
    CDS sequence
    • >TC010433 RA
      atgttgggg ttcggaaaa aagggtgct attagcaac 
      agtgccgaa aaacaagaa actctttta acgttatca 
      attctaact ttggcagcg atactttct tttgcgact 
      cgtcttttc tccgttttg cgttttgag agcgtaatc 
      cacgagttt gacccctat tttaattac cgcacgact 
      aaatttttg gcagaagag ggcttttat gcttttcat 
      aattggttc gatgacagg gcatggtat cccttgggg 
      aggattatc ggcggtacg atatatcct ggcttaatg 
      gtaacttca gcagccttt taccatttg tgttggtta 
      ttcaacatc acaattgat ataagaaat gtgtgcgtc 
      tttttggcg cccttattt tcgtctttt acgacaata 
      atcacatac ctgctgaca aaagaagtg aaaaatgcg 
      ggcgctggg cttgttgcc gcagctatg gtttctatt 
      gtaccagga tatatcagc agatcagtc gctgggagt 
      tacgataac gaaggaatt gctatattt tgtatgcta 
      ctcacttat tacacttgg attaaagcc gtgaaaact 
      gggacaatc ctgtggggg actttcgca gcactggca 
      tacttttac atggtctcc tcctgggga ggctacgtt 
      tttttaatc aatttaatc ccgttgcat gtcataact 
      ttgatggta actggccga ttttcacac agaatttac 
      atagcgtat agtactcta tattgtgtg gggactatt 
      ttatcaatg cagatttcc ttcgtgggc ttccagcct 
      gtgcaaagc tctgaacac atgctggcc ttgggcgtg 
      tttggctta tgtcaaatc tacgcgttc gtcgattac 
      ttgcgaagc aaactaacg aaaaacgac ttcgatgtt 
      ttattcaac ttcttaatt tacacagtg ggaatttta 
      ttcatcgta gttttcggc gttttgacc ctctctggg 
      aaaatttcc ccatggact ggtcgtttc tactccctc 
      cttgatcca tcatacgcc aaaaaccac attcccata 
      atcgccagc gtttccgaa catcagccc acgtcctgg 
      agctcgttt tatttcgat ttacaaatt ctggtcttc 
      ttgttccct gctggtctc tacttttgt ttcagtcag 
      ttgactgat gcgaacatt tttatgatt ttgtacggc 
      gttactagc atttatttt gctggggtc atggtgcgt 
      ctgatgtta gttctagcc ccagtcatg tgtatttta 
      agcggaatc gcagtctcg cacttgctt acaaaattc 
      atgaaaaac accgacacg gccaaacct tccgagcac 
      aaaaaaagc aaaaagttg gagtcgaat tacgtgctg 
      aaaaacgag atttcgtcg gcgtttgtt ggcgtcatt 
      tgcctgctg ttgatttct tacactttc cactgtact 
      tgggtcacc tccgaggcc tacagttcc ccgagtatt 
      gtgctaagt gcgaggtca cacgacggt ggaagaatc 
      attttcgat gatttccgc gaagcttac tactggctt 
      aagatgaac acgcctgaa gatgcgagg atcatgtcg 
      tggtgggat tacggttat cagataacg gcgatggcg 
      aaccggaca attctcgtc gataataac acttggaac 
      aacacgcat atcagtcga gtgggccag gcgatggcg 
      agctcggag gaaaaggcc tatgagatc atgaaggag 
      ctggatgtt gattacgtt ctagtaatt ttcgggggg 
      ctgacgggg tactcgtcc gatgatata aacaagttt 
      ttgtggatg gtccgcatt gggggaagt accgacaga 
      ggtgcgcat ataaaagag tggaattac tactcgtcc 
      agtggcgaa ttccgggtc gataaagaa gggtcgcaa 
      accctgctc aactgtctg atgtacaaa atgtgctac 
      taccgcttt gggcaagtg tacacggaa ggggggaag 
      cccccgggt tatgaccgc gtccgaggc gcggaaatt 
      ggaaataag gacttcgaa ctggacgtc ttggaggag 
      gcttacacg actgaacac tggctcgtg cgtatttac 
      aaagtcaaa gatttgccc aacaggggt gtataa
    mRNA sequence
    • >TC010433 RA
      ggactgtga tagaagaaa aagtgtttt aatttgtat 
      ttattttgc cgttttgta tatttttta aattttatt 
      ttgtgtatt aataaagaa ttacttcca aatttcttt 
      attatttct ttaaaacga atttttgcc ggccggtgt 
      ggagccggc aaagcgcgt gcgtttttg gaattgtgg 
      tgtgtgacg tgtatcggt aaaaaagtc ttttttgga 
      aatgtgcct gtttataca atgaaaaac cccactaac 
      gactaacaa tacgcgttc ccgtgcgtt tttaattat 
      tatttacac atttaagtg aatattaag taatttcca 
      taacctaac ctcagaaga cagttgcca caaaaaatt 
      aaaatgttg gggttcgga aaaaagggt gctattagc 
      aacagtgcc gaaaaacaa gaaactctt ttaacgtta 
      tcaattcta actttggca gcgatactt tcttttgcg 
      actcgtctt ttctccgtt ttgcgtttt gagagcgta 
      atccacgag tttgacccc tattttaat taccgcacg 
      actaaattt ttggcagaa gagggcttt tatgctttt 
      cataattgg ttcgatgac agggcatgg tatcccttg 
      gggaggatt atcggcggt acgatatat cctggctta 
      atggtaact tcagcagcc ttttaccat ttgtgttgg 
      ttattcaac atcacaatt gatataaga aatgtgtgc 
      gtctttttg gcgccctta ttttcgtct tttacgaca 
      ataatcaca tacctgctg acaaaagaa gtgaaaaat 
      gcgggcgct gggcttgtt gccgcagct atggtttct 
      attgtacca ggatatatc agcagatca gtcgctggg 
      agttacgat aacgaagga attgctata ttttgtatg 
      ctactcact tattacact tggattaaa gccgtgaaa 
      actgggaca atcctgtgg gggactttc gcagcactg 
      gcatacttt tacatggtc tcctcctgg ggaggctac 
      gttttttta atcaattta atcccgttg catgtcata 
      actttgatg gtaactggc cgattttca cacagaatt 
      tacatagcg tatagtact ctatattgt gtggggact 
      attttatca atgcagatt tccttcgtg ggcttccag 
      cctgtgcaa agctctgaa cacatgctg gccttgggc 
      gtgtttggc ttatgtcaa atctacgcg ttcgtcgat 
      tacttgcga agcaaacta acgaaaaac gacttcgat 
      gttttattc aacttctta atttacaca gtgggaatt 
      ttattcatc gtagttttc ggcgttttg accctctct 
      gggaaaatt tccccatgg actggtcgt ttctactcc 
      ctccttgat ccatcatac gccaaaaac cacattccc 
      ataatcgcc agcgtttcc gaacatcag cccacgtcc 
      tggagctcg ttttatttc gatttacaa attctggtc 
      ttcttgttc cctgctggt ctctacttt tgtttcagt 
      cagttgact gatgcgaac atttttatg attttgtac 
      ggcgttact agcatttat tttgctggg gtcatggtg 
      cgtctgatg ttagttcta gccccagtc atgtgtatt 
      ttaagcgga atcgcagtc tcgcacttg cttacaaaa 
      ttcatgaaa aacaccgac acggccaaa ccttccgag 
      cacaaaaaa agcaaaaag ttggagtcg aattacgtg 
      ctgaaaaac gagatttcg tcggcgttt gttggcgtc 
      atttgcctg ctgttgatt tcttacact ttccactgt 
      acttgggtc acctccgag gcctacagt tccccgagt 
      attgtgcta agtgcgagg tcacacgac ggtggaaga 
      atcattttc gatgatttc cgcgaagct tactactgg 
      cttaagatg aacacgcct gaagatgcg aggatcatg 
      tcgtggtgg gattacggt tatcagata acggcgatg 
      gcgaaccgg acaattctc gtcgataat aacacttgg 
      aacaacacg catatcagt cgagtgggc caggcgatg 
      gcgagctcg gaggaaaag gcctatgag atcatgaag 
      gagctggat gttgattac gttctagta attttcggg 
      gggctgacg gggtactcg tccgatgat ataaacaag 
      tttttgtgg atggtccgc attggggga agtaccgac 
      agaggtgcg catataaaa gagtggaat tactactcg 
      tccagtggc gaattccgg gtcgataaa gaagggtcg 
      caaaccctg ctcaactgt ctgatgtac aaaatgtgc 
      tactaccgc tttgggcaa gtgtacacg gaagggggg 
      aagcccccg ggttatgac cgcgtccga ggcgcggaa 
      attggaaat aaggacttc gaactggac gtcttggag 
      gaggcttac acgactgaa cactggctc gtgcgtatt 
      tacaaagtc aaagatttg cccaacagg ggtgtataa 
      gtagttttt caatttttc atttattaa taaaaaatc 
      actggtaat tgatgtttt atttgacct cgattagac 
      aaccaacaa tcgcatggt ggtagtgaa acagcaacc 
      ggcatgcgt cgaaaattg caaataaac tcttaggtt 
      acggagttg agctcattc gttcgtcta cgtttatca 
      catccttga attttagtg cgggaacta aattaaact 
      cgtgccaag acactgaat gggtttctc cgattcatc 
      aaatcaaaa tctttaatt ttcttgtaa gtgtcccgt 
      tttgttttt ttttttacc gcggtcgca attatttta 
      attt