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Gene information
General information
TC010426 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7350301..7352413
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010426
    iBeetle screen iB_01688

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010426 RA
      MHFKLLLLA ALVLLFSTA THASVGGYI CPNDRKKLL 
      TVLVKALIN VEGDFSTPY YGIKGFKAL NEQVAAVLV 
      KDNCAHIKK YYKADCTPE ENFQGLSAW NLLGCSGKL 
      HTDATITNL RTVLESDKS TTSDIRYAA ETLKLLGAA 
      IPNGAKVAQ LIQAKLKED DSLQSLGHA LHAAALLGT 
      SGNFIQDRI EEVVVQADE VDGKLLQWE GGLTTTSLL 
      ITGLLRFPG AKPLNAQQA EKLATYLLT RRTVQTPKG 
      ALALLEATT SLASSDISP VSITIAGPA QVTLDKPEL 
      KVHISDILG RALKAPPTP VVAQSATRI TDDVVVLSK 
      QPLSPGGTP VEFVLPLRL EPGQYRIAL TAGSHSTTL 
      TVRVLGPVT LEWLQIGLG DADGSAAPR LTKLTHPSK 
      LPSPLRADS SHHLVAKFA ISRVVHQAF LRLYSGKKE 
      IIFVAEPDN SKLYKISVN LASELAHSG TFEMELILG 
      DSVMSNPIR WVIGTIEVS LSTPEPEKK SLRGPKPEI 
      KHLFRQPEK RPAEVVSML FTGLTVSPL LLLFVLWWK 
      VGVNFGGFT TLSVPFHVG FGGILYLFF LFWWKLDMF 
      TTCAWLIPI GGFTFLAGH KLLSHVAKH KKA
    CDS sequence
    • >TC010426 RA
      atgcatttc aaacttcta ctattagct gcactcgta 
      cttttattc agcaccgcc acacatgca agcgttgga 
      ggttacatc tgccccaat gatcgaaaa aagttattg 
      actgtctta gttaaggct ctaatcaac gttgagggc 
      gacttttca acgccctat tatggcatt aaaggcttt 
      aaggctctt aacgaacaa gtggcggct gttctagtc 
      aaagataat tgtgcacac ataaagaaa tactacaaa 
      gctgactgc actcctgag gaaaatttc cagggtttg 
      tcggcctgg aacttgctg gggtgttcg ggaaaactt 
      cacactgat gccacaatt actaatttg cgaactgtg 
      ctggaaagt gacaaatcc acaacatcc gacattcga 
      tacgccgct gaaaccttg aaactgcta ggggccgca 
      atcccaaat ggggctaaa gtggcccaa ctcatccaa 
      gccaagtta aaggaagac gacagtttg caaagtttg 
      ggtcatgca ctacacgca gccgccctt ttgggcacc 
      tccggcaac ttcatccaa gaccgcatt gaagaagtg 
      gtcgtccaa gcggacgaa gtcgatgga aaactcctc 
      cagtgggaa gggggcctc accaccacc tccctcctc 
      ataaccggt ttgctccgt tttcccggg gccaagccc 
      ctcaacgcc caacaggcc gaaaaactg gccacttac 
      ttactcacc cgtcgaaca gtccagacc cccaagggg 
      gccctagct ttgctggaa gccaccaca agtttggct 
      tcaagcgac atctcccct gtcagcatc acaatcgcg 
      gggcccgct caagtcacc ttggacaaa ccggagctt 
      aaagtgcac atttcggat attctaggc agggctttg 
      aaggcgccc ccgacgcca gtcgtggcc caatctgcg 
      acacgcatc accgacgat gtcgtggtc ttgtccaag 
      cagcccctc agccctggg ggcacccca gttgagttc 
      gttttgccc ctgagactc gaaccgggg caatatcgc 
      attgcgctg actgcgggg tcacattcc accactctg 
      accgtcagg gttttgggg cccgtgacg ctcgagtgg 
      ctgcagatc gggctgggg gacgccgac ggcagtgca 
      gcccccagg ctcaccaaa ctaacacat ccttcgaaa 
      ttgcccagt ccgttacgt gcagattca agtcatcac 
      cttgttgcg aaatttgca atttcccga gtggtgcat 
      caagcattt ttgcggctt tattccggt aaaaaagag 
      attattttt gttgctgag cctgacaac agtaagttg 
      tacaaaatc agtgttaat ttagcgtca gaattggcg 
      cattcgggc acttttgaa atggaattg attttggga 
      gactctgtg atgagtaac cccattcgg tgggtcatt 
      ggtacgatc gaagttagt ttgagtaca cctgagccg 
      gagaaaaaa tcgctcaga ggccccaaa cccgagatt 
      aagcatctg ttcagacag ccggaaaaa cgaccagcg 
      gaagttgtg tcaatgctc ttcaccggt ttgacggtt 
      tcccccttg ctactgttg ttcgttttg tggtggaaa 
      gttggggtc aattttggc ggtttcacc acgctttcg 
      gtaccattc catgtcggt tttgggggc attttgtac 
      ttgtttttc ctgttttgg tggaaacta gacatgttc 
      accacttgt gcttggttg attccaatc ggaggtttc 
      acgtttttg gccggtcac aagttactg agccatgtc 
      gcaaaacac aaaaaagct tga
    mRNA sequence
    • >TC010426 RA
      ctaccaaca gtcagattt tgtgatgta cgtgtcgtt 
      gttgtaatg tgccggtga tttccacaa taaaatgca 
      tttcaaact tctactatt agctgcact cgtactttt 
      attcagcac cgccacaca tgcaagcgt tggaggtta 
      catctgccc caatgatcg aaaaaagtt attgactgt 
      cttagttaa ggctctaat caacgttga gggcgactt 
      ttcaacgcc ctattatgg cattaaagg ctttaaggc 
      tcttaacga acaagtggc ggctgttct agtcaaaga 
      taattgtgc acacataaa gaaatacta caaagctga 
      ctgcactcc tgaggaaaa tttccaggg tttgtcggc 
      ctggaactt gctggggtg ttcgggaaa acttcacac 
      tgatgccac aattactaa tttgcgaac tgtgctgga 
      aagtgacaa atccacaac atccgacat tcgatacgc 
      cgctgaaac cttgaaact gctaggggc cgcaatccc 
      aaatggggc taaagtggc ccaactcat ccaagccaa 
      gttaaagga agacgacag tttgcaaag tttgggtca 
      tgcactaca cgcagccgc ccttttggg cacctccgg 
      caacttcat ccaagaccg cattgaaga agtggtcgt 
      ccaagcgga cgaagtcga tggaaaact cctccagtg 
      ggaaggggg cctcaccac cacctccct cctcataac 
      cggtttgct ccgttttcc cggggccaa gcccctcaa 
      cgcccaaca ggccgaaaa actggccac ttacttact 
      cacccgtcg aacagtcca gacccccaa gggggccct 
      agctttgct ggaagccac cacaagttt ggcttcaag 
      cgacatctc ccctgtcag catcacaat cgcggggcc 
      cgctcaagt caccttgga caaaccgga gcttaaagt 
      gcacatttc ggatattct aggcagggc tttgaaggc 
      gcccccgac gccagtcgt ggcccaatc tgcgacacg 
      catcaccga cgatgtcgt ggtcttgtc caagcagcc 
      cctcagccc tgggggcac cccagttga gttcgtttt 
      gcccctgag actcgaacc ggggcaata tcgcattgc 
      gctgactgc ggggtcaca ttccaccac tctgaccgt 
      cagggtttt ggggcccgt gacgctcga gtggctgca 
      gatcgggct gggggacgc cgacggcag tgcagcccc 
      caggctcac caaactaac acatccttc gaaattgcc 
      cagtccgtt acgtgcaga ttcaagtca tcaccttgt 
      tgcgaaatt tgcaatttc ccgagtggt gcatcaagc 
      atttttgcg gctttattc cggtaaaaa agagattat 
      ttttgttgc tgagcctga caacagtaa gttgtacaa 
      aatcagtgt taatttagc gtcagaatt ggcgcattc 
      gggcacttt tgaaatgga attgatttt gggagactc 
      tgtgatgag taaccccat tcggtgggt cattggtac 
      gatcgaagt tagtttgag tacacctga gccggagaa 
      aaaatcgct cagaggccc caaacccga gattaagca 
      tctgttcag acagccgga aaaacgacc agcggaagt 
      tgtgtcaat gctcttcac cggtttgac ggtttcccc 
      cttgctact gttgttcgt tttgtggtg gaaagttgg 
      ggtcaattt tggcggttt caccacgct ttcggtacc 
      attccatgt cggttttgg gggcatttt gtacttgtt 
      tttcctgtt ttggtggaa actagacat gttcaccac 
      ttgtgcttg gttgattcc aatcggagg tttcacgtt 
      tttggccgg tcacaagtt actgagcca tgtcgcaaa 
      acacaaaaa agcttgagc tgtgcaatt tttgcaatt 
      tgagtgtcc aattttgtt gtttgaatt gtttaattt 
      gcccaccgg caatttgta aagactttt gtaataaat 
      aaaatacag tacgtgtat agta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY