Status
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Gene information
General information
TC010423 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7399862..7401733
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010423
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01687

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    antenna shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    antenna not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs sometimes randomnot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax segment sometimes partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen constricted - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010423 RA
      MNIILEFFS ALAVIIVLS WWYLFLKNY KVGKFFKNF 
      SGPPGVPLL GNALDFKNK TKGVLPAFL NYNKNFGGL 
      VKVQIGPFR KLLLVSDYK FLEFVLSST KIINKSQNY 
      RTMLPWLGT GLLTSEGIK WKKHRRIIT PTFHFKILE 
      QFINSFDAA GDVMINKLR KKVGIESVD IYPFVTLCA 
      LDIICETAM GTTINAQNN EESEYVTSV KEMGRIIIE 
      RAIAPQKNN EFLFRFTKD YQLQKSALK VLHNYTNNV 
      ISKRREELL KDQASKVSE NVIDMGIKK KMAFLDLLL 
      QATVDGRPL TNEEIREEV DTFMFEGHD TTASGISFA 
      LYCLANNPE AQEKAYEEQ VALFGKEKK PIVSYSDLQ 
      EMKYLELVI KEALRLYPS VPFYARETN QEVEFGDIK 
      IPKGVNITI FAYGIHRDP KYFPEPDKF DPGRFETID 
      GKLPYAYIP FSAGPRNCI GQKFAMLEM KSTISKVLR 
      NFKLCPATP HHTLDLVAE TVLKSDNGV RLSLMERQ
    CDS sequence
    • >TC010423 RA
      atgaacatc atacttgaa tttttctca gccttggct 
      gtgatcata gtgttgtcg tggtggtac ctcttctta 
      aaaaactac aaagttggc aaatttttc aaaaatttt 
      tccggccct ccgggcgtt ccactcctc ggcaatgcg 
      ctcgacttc aagaataaa accaaaggc gtcttgccg 
      gctttcctc aattataac aaaaacttc ggcggactt 
      gtgaaagtc caaatcgga ccgttccgg aaactgctc 
      ctagtctcg gactacaaa tttctggaa ttcgtctta 
      agttcgacg aaaattatc aacaaatcg cagaattac 
      cggacaatg ttgccctgg ctgggcacc ggcttgctc 
      acaagtgag ggaattaag tggaagaaa cacagaagg 
      attatcaca ccgacgttc catttcaaa attttggaa 
      caatttatc aattccttc gacgctgct ggcgatgtt 
      atgatcaat aaactgcga aagaaggtg ggaattgag 
      agcgtcgat atttaccct tttgtcaca ctttgtgcc 
      ttagatatt atttgtgaa acagcaatg ggtacgact 
      ataaatgcc caaaacaac gaagaatcc gagtatgtc 
      acaagtgtg aaagaaatg ggccgtatt atcatcgaa 
      cgtgcaatt gccccacaa aaaaataac gaatttttg 
      ttccgcttc actaaagac taccagctt cagaaaagt 
      gcgttgaaa gtcttgcac aattacacc aacaacgtg 
      atcagcaaa cgacgggaa gaactccta aaagaccaa 
      gcgtccaaa gtaagcgag aatgtgatc gacatgggg 
      attaagaaa aaaatggcc tttttggac ctgcttttg 
      caagccact gtcgacgga cggcctcta accaacgaa 
      gaaataaga gaagaagtt gacacgttc atgtttgaa 
      ggtcacgac accacggct tcaggcatc agttttgca 
      ctgtattgt ctagccaac aatcccgaa gctcaggaa 
      aaggcgtac gaagaacaa gtggcactt ttcggcaaa 
      gagaagaaa ccgattgtg agctactcc gacctgcag 
      gaaatgaag tatttggag cttgtgatc aaagaagcg 
      ctccgtttg tacccctcg gtgccgttc tacgcccgg 
      gaaaccaac caggaggtc gagttcggc gatattaag 
      atccccaaa ggtgtgaat atcacaatt ttcgcttac 
      ggcatccac cgggacccg aaatatttc ccagagccg 
      gacaagttc gatcccggc agattcgaa acgatcgat 
      ggcaaactg ccttatgct tatataccg ttcagtgcc 
      ggccctagg aattgcatt gggcaaaaa ttcgcaatg 
      ttggagatg aaaagcacg atttcgaag gtgttgagg 
      aatttcaaa ctgtgtcca gcgacgccc catcacacg 
      ttggacctt gtggcggaa actgtactt aagtccgac 
      aatggggtt cgactttcg ctgatggag aggcaataa
    mRNA sequence
    • >TC010423 RA
      accagaaca gtttacacc ctcagcatg aacatcata 
      cttgaattt ttctcagcc ttggctgtg atcatagtg 
      ttgtcgtgg tggtacctc ttcttaaaa aactacaaa 
      gttggcaaa tttttcaaa aatttttcc ggccctccg 
      ggcgttcca ctcctcggc aatgcgctc gacttcaag 
      aataaaacc aaaggcgtc ttgccggct ttcctcaat 
      tataacaaa aacttcggc ggacttgtg aaagtccaa 
      atcggaccg ttccggaaa ctgctccta gtctcggac 
      tacaaattt ctggaattc gtcttaagt tcgacgaaa 
      attatcaac aaatcgcag aattaccgg acaatgttg 
      ccctggctg ggcaccggc ttgctcaca agtgaggga 
      attaagtgg aagaaacac agaaggatt atcacaccg 
      acgttccat ttcaaaatt ttggaacaa tttatcaat 
      tccttcgac gctgctggc gatgttatg atcaataaa 
      ctgcgaaag aaggtggga attgagagc gtcgatatt 
      taccctttt gtcacactt tgtgcctta gatattatt 
      tgtgaaaca gcaatgggt acgactata aatgcccaa 
      aacaacgaa gaatccgag tatgtcaca agtgtgaaa 
      gaaatgggc cgtattatc atcgaacgt gcaattgcc 
      ccacaaaaa aataacgaa tttttgttc cgcttcact 
      aaagactac cagcttcag aaaagtgcg ttgaaagtc 
      ttgcacaat tacaccaac aacgtgatc agcaaacga 
      cgggaagaa ctcctaaaa gaccaagcg tccaaagta 
      agcgagaat gtgatcgac atggggatt aagaaaaaa 
      atggccttt ttggacctg cttttgcaa gccactgtc 
      gacggacgg cctctaacc aacgaagaa ataagagaa 
      gaagttgac acgttcatg tttgaaggt cacgacacc 
      acggcttca ggcatcagt tttgcactg tattgtcta 
      gccaacaat cccgaagct caggaaaag gcgtacgaa 
      gaacaagtg gcacttttc ggcaaagag aagaaaccg 
      attgtgagc tactccgac ctgcaggaa atgaagtat 
      ttggagctt gtgatcaaa gaagcgctc cgtttgtac 
      ccctcggtg ccgttctac gcccgggaa accaaccag 
      gaggtcgag ttcggcgat attaagatc cccaaaggt 
      gtgaatatc acaattttc gcttacggc atccaccgg 
      gacccgaaa tatttccca gagccggac aagttcgat 
      cccggcaga ttcgaaacg atcgatggc aaactgcct 
      tatgcttat ataccgttc agtgccggc cctaggaat 
      tgcattggg caaaaattc gcaatgttg gagatgaaa 
      agcacgatt tcgaaggtg ttgaggaat ttcaaactg 
      tgtccagcg acgccccat cacacgttg gaccttgtg 
      gcggaaact gtacttaag tccgacaat ggggttcga 
      ctttcgctg atggagagg caataacat attgttatg 
      tctgttatt tatcagtat ctaattttt atatgtttt 
      attattgtt ttaataaat aaactattt ttctaaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY