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Gene information
General information
TC010419 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 6387925..6396245
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010419
    iBeetle screen iB_01686
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010419 RA
      MLRELTLLV VGAAMVMAQ RRLALPDPR 
      SCASRVRHA TYRDARGVT HSYFFSWEH 
      PPTRSLEVD WLDARNICR RHCMDAVSL 
      ETPQENEFI KQRLVRGNV RYIWTSGRK 
      CNFAGCERA DLQPPNING WFWSGSGAK 
      IGPTTQRNS GDWSHTGGY GQPQPDNRE 
      AAQGNDESC LSILNNFYN DGVKWHDVA 
      CYHVKPFVC EDSEELLNF VASRNPGIR 
      L
    CDS sequence
    • >TC010419 RA
      atgcttcga gaactgacg ctccttgtg gtaggagcc 
      gccatggtg atggcccaa agacgactg gccctccca 
      gaccccaga agttgtgcc agcagagtt cgccacgcc 
      acttacaga gacgcccgc ggagtgaca cattcgtac 
      ttcttcagc tgggagcat ccgcccacc agaagcctg 
      gaagtggac tggcttgac gcccgtaac atctgcaga 
      agacactgc atggacgcc gtgtcgctg gaaacacct 
      caagagaac gaattcatc aaacagaga ctagtcaga 
      ggcaacgtc cgctacatc tggacctcg ggccgaaaa 
      tgcaacttc gccggttgc gagcgcgcc gacttgcag 
      cctccaaac atcaacgga tggttctgg tccggatcc 
      ggggccaag atcgggccc accactcag agaaactcc 
      ggggattgg agtcacacc ggagggtac ggccaaccc 
      cagcccgac aacagagag gctgcacag ggcaatgat 
      gaatcttgc ttgtccatt ttgaacaac ttttacaat 
      gacggggtt aaatggcac gatgttgcg tgctaccat 
      gtcaagcct ttcgtttgt gaagacagt gaagagttg 
      ttgaacttt gttgcttcc aggaatcca ggaattcgt 
      ctttaa
    mRNA sequence
    • >TC010419 RA
      ttcagtgcc acagcaaag gttcaatgt gtaatttac 
      ataaacaaa taaatacgc gtttaatgt ctcttttta 
      ttattagaa tgtgatgcg aattcggtc aatgtgaaa 
      ccaacaatg aaaaatatc cacaaatcg atcttcgta 
      gccttggga gtagcgttc cggaaacgt tttttcttg 
      ctgaacaat taaatatta tttaaattg taacagttc 
      ggaattgaa aattttgca caaggtctg aagatctcc 
      tctcgaggt atgcgatag tgatagtgg tcaaatgtc 
      agtggtcat cggacgccg cttgggggt actcggggg 
      gtccccggg gggttccat cgaatgtag gttatggag 
      ttgtgggga tgtaggacg gctatatac accgactct 
      gcatctgtc agacagttg attcaaagt gcttcttca 
      ctgctactt gctggaata ttacaggcc aaaatgctt 
      cgagaactg acgctcctt gtggtagga gccgccatg 
      gtgatggcc caaagacga ctggccctc ccagacccc 
      agaagttgt gccagcaga gttcgccac gccacttac 
      agagacgcc cgcggagtg acacattcg tacttcttc 
      agctgggag catccgccc accagaagc ctggaagtg 
      gactggctt gacgcccgt aacatctgc agaagacac 
      tgcatggac gccgtgtcg ctggaaaca cctcaagag 
      aacgaattc atcaaacag agactagtc agaggcaac 
      gtccgctac atctggacc tcgggccga aaatgcaac 
      ttcgccggt tgcgagcgc gccgacttg cagcctcca 
      aacatcaac ggatggttc tggtccgga tccggggcc 
      aagatcggg cccaccact cagagaaac tccggggat 
      tggagtcac accggaggg tacggccaa ccccagccc 
      gacaacaga gaggctgca cagggcaat gatgaatct 
      tgcttgtcc attttgaac aacttttac aatgacggg 
      gttaaatgg cacgatgtt gcgtgctac catgtcaag 
      cctttcgtt tgtgaagac agtgaagag ttgttgaac 
      tttgttgct tccaggaat ccaggaatt cgtctttaa 
      attatttct ttaacagtc ctttcgttc ttcctcagt 
      gttttggag aaaatttaa ccacggatc gtggtttgt 
      tctcttcaa gttcccttt ctgcatgtg aatatctgc 
      ttcctttcc ctagtttta gtaattatt gtatctatt 
      tttaaatta tattttaat attaaacat ttatattaa 
      ac