Status
Please wait ...
Gene information
General information
TC010417 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7450008..7459978
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010417
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01685

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010417 RA
      MAANPFDKY LKTLTVESK EYKYYDLSA LGAQYDRLP 
      YSIRVLLES AVRNCDNFQ VKENDVQNI LNWEQNQSV 
      EGGIEIPFK PARVILQDF TGVPAVVDF AAMRDAVKG 
      LGGNPEKIN PSCPADLVI DHSVQVDFA RSPSALKKN 
      EDLEFERNQ ERFTFLKWG AKAFNNMLI VPPGSGIVH 
      QVNLEYLAR VVFTGKDKP ILYPDTVVG TDSHTTMIN 
      GLGVLGWGV GGIEAEAVM LGQSISMLL PKVVGYRLH 
      GTLGQYVTS TDLVLTITK NLRQLGVVG KFVEFYGPG 
      VAALSIADR ATIANMCPE YGATVGYFP VDEHSLTYL 
      RQTSRPDEQ IKLIEAYLK ATKQLRNYA NEMNEPIFS 
      QSVSLDLST VVSSVSGPK RPNDRVSVS DMKNDFRLC 
      LSNKIGFKG FGIPEAKLN TEAKFMYNG SQYTIRHGS 
      VIIAAITSC TNTSNPSVM LGAGLLAKN AVAAGLTVA 
      PYIKTSLSP GSGVVTYYL QESKVIDAL TQLGFDIVG 
      YGCMTCIGN SGGIDENIV NAIEQNDLV CCGVLSGNR 
      NFEGRIHPN TRANYLASP LLVIAYAIA GTVDIDFEK 
      EPLGKRPDG SPVFLREIW PTRKEIHAV EQQYVIPAM 
      FQQVYSRIQ LGSSSWQSL NAPSGILYP WSDSSTYIK 
      KPPFFDGMT KQLPPMQPI SGARVLLYL GDSVTTDHI 
      SPAGSIGRN SPAARYLAQ NGLTPREFN SYGSRRGND 
      AIMARGTFA NIRLVNKFM SNAGPKTVY LPTNEEMDV 
      FDCAERYKS AKTPLIILA GKDYGSGSS RDWAAKGPY 
      LLGVRAVIA ESFERIHRS NLVGMGIIP LQFLPNETA 
      ETLGLTGKE IYNIEIPAD LKPGQNIKI STDTSKTFN 
      VVLRFDTEV DLLFYKHGG ILNYMIRKI VA
    CDS sequence
    • >TC010417 RA
      atggctgcc aaccctttt gacaaatat ttgaaaact 
      ttaacagta gagtctaag gagtacaag tattatgat 
      ttatcggca ctgggggcc caatatgac cggctgccc 
      tattctatc agagtcttg ctggagtca gcggtcaga 
      aactgtgac aatttccaa gttaaagaa aacgatgtt 
      caaaatatc ctaaactgg gaacaaaat cagtcggtg 
      gaaggtggc attgagata ccattcaaa cctgcaagg 
      gtcattctg caggacttt acgggggtc cctgcagtg 
      gtcgacttt gccgcaatg agagacgct gttaaaggt 
      ttgggcgga aatccggaa aaaattaat ccaagttgt 
      cctgcagac ctcgttatt gatcattct gttcaagtt 
      gattttgca agatcgcca agtgcgtta aagaagaat 
      gaagatttg gaatttgag cgaaatcag gaaagattc 
      acttttctg aaatggggg gctaaagcg ttcaataac 
      atgttgatt gtgccacca gggagtgga attgtgcac 
      caagtcaac ttggagtac ttggcaagg gttgttttc 
      accgggaaa gacaagccc attttgtac ccagataca 
      gtggtcgga actgactcg cacacgact atgatcaac 
      ggattaggt gttctgggt tggggtgtt ggtggtatt 
      gaagctgag gctgttatg ttaggccaa tccatcagt 
      atgttactc ccaaaggtg gttggttac agattgcac 
      ggtacttta ggacaatat gtgacctcc actgattta 
      gttctcaca ataactaaa aatttgaga caattggga 
      gttgttgga aagtttgtc gagttttac ggtccagga 
      gtggcagca ctgtcgatc gcagaccgt gccacaatc 
      gctaatatg tgtcccgaa tatggggct actgttgga 
      tatttcccc gttgatgag cattctctg acttatttg 
      agacaaact agtcgccca gatgaacag attaaattg 
      attgaggcg tatttgaaa gctacgaag caattgaga 
      aattatgcg aatgaaatg aatgaacca attttcagc 
      cagagcgtg agcttggac ttatcaact gttgtctcg 
      tctgtgagc ggaccgaaa cgacctaac gacagagtt 
      tctgtttca gatatgaag aacgatttc agattgtgt 
      ttaagcaat aagatcggt ttcaagggc ttcggcatc 
      cccgaagcc aaactcaac accgaagcc aaattcatg 
      tacaacggc tcgcaatac acgatccgc cacggaagc 
      gtaataatc gcagcaata acctcgtgc acaaacaca 
      agcaatccc agcgtgatg ctgggggcc ggcctcttg 
      gccaagaac gcggtggct gccggcctc accgtcgcc 
      ccttacatc aaaacgagc ctttccccc ggctcgggg 
      gtcgtcact tactacctc caggagtcc aaagtcatc 
      gacgcccta acccagctc gggttcgac atcgtgggc 
      tacggctgc atgacctgc attggcaac agcggcggc 
      attgacgaa aacattgtc aatgcaatc gagcaaaac 
      gatttagtt tgttgtggc gtactttcc gggaataga 
      aatttcgaa gggcggatt cacccaaac acgcgggct 
      aattactta gcgagtccg ctcctagtc atagcctat 
      gccattgcg ggcacggtg gacatagac tttgagaag 
      gagcctttg gggaaacgg ccggacggg tctccggtc 
      tttttgcgc gaaatttgg ccgacgagg aaggaaatt 
      catgcagtg gagcaacaa tatgtgatc ccggcaatg 
      ttccagcag gtgtattcg cgaattcag ttgggttcg 
      tcaagttgg caatcgttg aatgcacct tctgggatt 
      ttgtacccc tggtctgac agttccacc tacattaaa 
      aaaccgccg tttttcgac gggatgacg aagcagttg 
      cccccaatg cagcccatt tcgggggcc agggttttg 
      ctctatttg ggggattcg gtcaccact gaccacatt 
      agccctgcc ggctccatt ggacggaac agtccagct 
      gcgcggtat ttggcccag aatgggtta actccgcgc 
      gaatttaat tcttatggg tcgcgaagg ggcaacgat 
      gctatcatg gcaaggggc actttcgct aatatacga 
      ctggttaac aagtttatg agtaatgca ggcccgaag 
      actgtgtat ttgcccaca aatgaggag atggatgtt 
      tttgattgt gccgagagg tacaagagt gccaagacc 
      ccgctgatt atattagct ggaaaggat tacgggtcc 
      ggctccagc agggattgg gcggccaag ggaccctac 
      ttgttggga gtgcgtgcc gtaatcgct gagtcattc 
      gaacgaatt catcgctcg aatttggtg ggtatgggt 
      attattcct ctccaattc ttgccgaac gaaacggcc 
      gaaactctc ggcctaaca gggaaggaa atttacaat 
      atcgaaatt ccggccgat ttaaaaccg ggacaaaac 
      attaaaatt tccacagat acgagcaaa acgttcaat 
      gtcgttttg cgattcgat actgaggtt gacttattg 
      ttctacaaa cacggcggt atcttgaat tacatgatc 
      aggaaaatc gtagcgtaa
    mRNA sequence
    • >TC010417 RA
      cacaacaaa aatcaaaaa tcactttta taaaaaatt 
      gtggagcgc tgaaactgc gcaaaagac tctgaaaca 
      taacctgta catttcacc atacttttt aaagtttta 
      atcaaatac aattgtttg aattgtgga ttgacctct 
      gaaagcagt gataacgta tatcagata attatttgt 
      caaaaaata agtgcgttt tgcggtttt cgtggtggt 
      tttagatcg acaaacatt tttgttatt ctggcaact 
      gctacgtga cgggcaagt tgtaagtca tggctgcca 
      acccttttg acaaatatt tgaaaactt taacagtag 
      agtctaagg agtacaagt attatgatt tatcggcac 
      tgggggccc aatatgacc ggctgccct attctatca 
      gagtcttgc tggagtcag cggtcagaa actgtgaca 
      atttccaag ttaaagaaa acgatgttc aaaatatcc 
      taaactggg aacaaaatc agtcggtgg aaggtggca 
      ttgagatac cattcaaac ctgcaaggg tcattctgc 
      aggacttta cgggggtcc ctgcagtgg tcgactttg 
      ccgcaatga gagacgctg ttaaaggtt tgggcggaa 
      atccggaaa aaattaatc caagttgtc ctgcagacc 
      tcgttattg atcattctg ttcaagttg attttgcaa 
      gatcgccaa gtgcgttaa agaagaatg aagatttgg 
      aatttgagc gaaatcagg aaagattca cttttctga 
      aatgggggg ctaaagcgt tcaataaca tgttgattg 
      tgccaccag ggagtggaa ttgtgcacc aagtcaact 
      tggagtact tggcaaggg ttgttttca ccgggaaag 
      acaagccca ttttgtacc cagatacag tggtcggaa 
      ctgactcgc acacgacta tgatcaacg gattaggtg 
      ttctgggtt ggggtgttg gtggtattg aagctgagg 
      ctgttatgt taggccaat ccatcagta tgttactcc 
      caaaggtgg ttggttaca gattgcacg gtactttag 
      gacaatatg tgacctcca ctgatttag ttctcacaa 
      taactaaaa atttgagac aattgggag ttgttggaa 
      agtttgtcg agttttacg gtccaggag tggcagcac 
      tgtcgatcg cagaccgtg ccacaatcg ctaatatgt 
      gtcccgaat atggggcta ctgttggat atttccccg 
      ttgatgagc attctctga cttatttga gacaaacta 
      gtcgcccag atgaacaga ttaaattga ttgaggcgt 
      atttgaaag ctacgaagc aattgagaa attatgcga 
      atgaaatga atgaaccaa ttttcagcc agagcgtga 
      gcttggact tatcaactg ttgtctcgt ctgtgagcg 
      gaccgaaac gacctaacg acagagttt ctgtttcag 
      atatgaaga acgatttca gattgtgtt taagcaata 
      agatcggtt tcaagggct tcggcatcc ccgaagcca 
      aactcaaca ccgaagcca aattcatgt acaacggct 
      cgcaataca cgatccgcc acggaagcg taataatcg 
      cagcaataa cctcgtgca caaacacaa gcaatccca 
      gcgtgatgc tgggggccg gcctcttgg ccaagaacg 
      cggtggctg ccggcctca ccgtcgccc cttacatca 
      aaacgagcc tttcccccg gctcggggg tcgtcactt 
      actacctcc aggagtcca aagtcatcg acgccctaa 
      cccagctcg ggttcgaca tcgtgggct acggctgca 
      tgacctgca ttggcaaca gcggcggca ttgacgaaa 
      acattgtca atgcaatcg agcaaaacg atttagttt 
      gttgtggcg tactttccg ggaatagaa atttcgaag 
      ggcggattc acccaaaca cgcgggcta attacttag 
      cgagtccgc tcctagtca tagcctatg ccattgcgg 
      gcacggtgg acatagact ttgagaagg agcctttgg 
      ggaaacggc cggacgggt ctccggtct ttttgcgcg 
      aaatttggc cgacgagga aggaaattc atgcagtgg 
      agcaacaat atgtgatcc cggcaatgt tccagcagg 
      tgtattcgc gaattcagt tgggttcgt caagttggc 
      aatcgttga atgcacctt ctgggattt tgtacccct 
      ggtctgaca gttccacct acattaaaa aaccgccgt 
      ttttcgacg ggatgacga agcagttgc ccccaatgc 
      agcccattt cgggggcca gggttttgc tctatttgg 
      gggattcgg tcaccactg accacatta gccctgccg 
      gctccattg gacggaaca gtccagctg cgcggtatt 
      tggcccaga atgggttaa ctccgcgcg aatttaatt 
      cttatgggt cgcgaaggg gcaacgatg ctatcatgg 
      caaggggca ctttcgcta atatacgac tggttaaca 
      agtttatga gtaatgcag gcccgaaga ctgtgtatt 
      tgcccacaa atgaggaga tggatgttt ttgattgtg 
      ccgagaggt acaagagtg ccaagaccc cgctgatta 
      tattagctg gaaaggatt acgggtccg gctccagca 
      gggattggg cggccaagg gaccctact tgttgggag 
      tgcgtgccg taatcgctg agtcattcg aacgaattc 
      atcgctcga atttggtgg gtatgggta ttattcctc 
      tccaattct tgccgaacg aaacggccg aaactctcg 
      gcctaacag ggaaggaaa tttacaata tcgaaattc 
      cggccgatt taaaaccgg gacaaaaca ttaaaattt 
      ccacagata cgagcaaaa cgttcaatg tcgttttgc 
      gattcgata ctgaggttg acttattgt tctacaaac 
      acggcggta tcttgaatt acatgatca ggaaaatcg 
      tagcgtaat caaaagggt tttggtaaa cttttttca 
      atggattgg aagttctat tttattact taatctagt 
      acaaaatat tgttggaag taagctcag ttttattgt 
      tgagggttt atatttttg tgtgggctt ctttactta 
      gttgttata tatttttaa caatgttac acttttata 
      taaattgat gataataat aaaccatca cttttaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY