Status
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Gene information
General information
TC010410 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7520088..7523138
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010410
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01684

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 62.5% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    remarks: animals lost,
    Pupal morphology
    antenna shortened - Number of animals showing the phenotype: 7

    Adult morphology
    antenna shortened - Number of animals showing the phenotype: 5


    remarks: animals lost,
    Fertility
    egg production: number no/almost no eggs -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010410 RA
      MENSAPSLA ITPNVLLAE CCSICGKAH STQNCDYVQ 
      ILKYVHDNE IPSRAKLTL PKFLSIRIL SDGSYATIT 
      KSTISKGTR FGPFQAEKS STLEPTMTF PLKIFDKEN 
      REYFLDATD EQKCSWLMF VGHADCVEE QNLICYQEK 
      GEIYYVAIK DIRIGETLK VWYAPYYAQ KMNKAILAP 
      KVADDPLKN VDIDFLIKK QQKITERKV WTCKFCSHI 
      ENNITSFAR HILKHYTAQ HKNMCELCK KSFRSKKGL 
      NNHLKIVHK MLVTSSPKT SKSVVTPPK TDCRDVVLG 
      GPLLNDMFT DSSDLQQFD LLDNQNLLL DNDNLTVEN 
      LLSDNVKDL DHFNFEIDD SQKQFICDI CLKQFTKIK 
      LLIVHLRHH TGDFMCYKC LKIFCRREN LKSHICNMS 
      SHHRCDLCD RTFTQRKYL SRHLEAVHN KKYSCVSCG 
      KNYHSNKEL LEHNCSKIP NADKERFMC PICDKLFFR 
      EIYLKRHLK THDQKGRTS KPNYVTCDV CGLHLISTS 
      YFSHKKKHQ EPSYACEIC SKKFHRTDS LKQHVLIHQ 
      ANEFECDIC HKKYKTEKV FKYHLKRHN SVQAYKCSL 
      CDASFKIRN SLNRHIKNF HREKSNAKS EIEYYICPV 
      CQKSIKLRS SLLRHLKKS HPEVANIDL NAIRPKLFK 
      NTKNNDKDN EVPFSLTNE IDNQINTLL NNAENNTTI 
      NKNAVDSLV EELDINNKN EDKETREVC LSIPDLTEN 
      YQEITLDKN AYILDNGTI VQPQANSEN VIIYVLDQT 
      Y
    CDS sequence
    • >TC010410 RA
      atggaaaat tcggcccct tctcttgcc atcacgcca 
      aacgtgtta cttgccgaa tgttgcagt atctgtgga 
      aaagcgcac agtacccaa aattgtgat tacgtacaa 
      attttgaaa tatgtccat gataatgaa ataccatcc 
      agggctaaa ctcacttta cccaaattt ttaagcata 
      cgaattcta agtgatggc tcatatgca acaattacc 
      aagtctaca ataagtaag gggaccaga tttggccct 
      ttccaggcc gaaaaatcc agtactttg gagcctacg 
      atgactttt cctttgaaa atttttgat aaagaaaat 
      cgcgagtat tttttagat gccaccgac gaacagaag 
      tgcagttgg ctgatgttt gtggggcac gctgattgt 
      gttgaagaa cagaattta atctgctat caggaaaaa 
      ggcgaaatt tactacgtt gcaattaag gatattagg 
      attggggag actcttaag gtgtggtat gccccctac 
      tatgctcaa aaaatgaac aaagcaata ttagcccca 
      aaagtagcc gacgatcct ttaaaaaat gtggacatt 
      gattttttg attaaaaag caacaaaaa ataacggaa 
      cgtaaagtc tggacctgt aagttttgt agtcacatt 
      gagaacaat attacttcg tttgcgagg catatcttg 
      aaacattac actgctcag cacaaaaac atgtgcgaa 
      ttgtgtaag aaatcgttc agatcaaag aaaggctta 
      aataatcat ttgaaaatc gtgcacaaa atgttagtg 
      acttcgtca cccaaaact tcgaaaagt gttgttacg 
      cccccgaaa acagattgc agagatgtg gtacttggg 
      ggtccttta ttgaacgat atgttcacg gacagttcc 
      gatttgcaa caatttgat ctcctagat aatcagaat 
      ttattattg gacaatgat aatctaaca gtggaaaat 
      ctgctcagt gacaatgtt aaagatttg gaccatttc 
      aatttcgag atagacgac agtcagaag caatttatt 
      tgtgatatt tgtcttaag caatttacc aaaatcaag 
      ctcctaatc gtgcatttg aggcaccat acaggggac 
      tttatgtgt tacaagtgt ctaaagatt ttttgccga 
      agggaaaat ttaaaatcg cacatttgc aacatgagc 
      agtcaccac cgctgcgat ttatgcgat cgtactttc 
      acccaacgt aaatacctc tcgcggcat ctggaagcc 
      gtccataat aaaaaatac agttgcgtt tcgtgtggc 
      aaaaactat cactcgaat aaggagttg ctggaacac 
      aactgtagc aaaatcccg aatgccgac aaggagcgc 
      tttatgtgc cccatttgt gacaaactc ttcttccgt 
      gaaatttat ctcaagaga catttgaaa actcacgac 
      caaaaaggc agaacttca aagccgaat tacgtaact 
      tgcgacgtc tgcggcttg cacttaatc agcacttcg 
      tatttctcg cacaagaag aaacaccaa gagccttca 
      tacgcttgc gaaatctgt agtaaaaaa ttccacagg 
      accgactcg ctcaaacaa cacgttttg atccatcaa 
      gccaacgag ttcgaatgt gatatttgc cataaaaag 
      tacaaaacc gaaaaagta tttaagtac catctcaag 
      cggcataat agtgtgcag gcctacaag tgctcgctg 
      tgcgacgcc tcgttcaaa atccgcaac agtttaaac 
      cgacacatt aagaatttt cacagagaa aaaagcaat 
      gcaaagagc gagattgaa tactacatt tgcccagtt 
      tgccaaaag tcaattaaa ttgcgaagc tcgctgttg 
      aggcatttg aagaaatcg caccctgaa gtggccaac 
      attgacttg aacgcaatt agacccaaa ttatttaaa 
      aacacaaaa aataatgat aaggataat gaagtgccg 
      tttagtctt acgaacgaa atcgataac caaattaat 
      acgttgttg aacaatgcc gaaaataac acaacgatt 
      aataaaaac gccgttgat agtttggtg gaagagctg 
      gatattaat aacaaaaat gaggacaaa gagacgagg 
      gaagtgtgt ctctcgatt ccggacctt accgaaaat 
      taccaggaa atcacctta gataaaaac gcttatatc 
      ctggataat ggcaccatt gttcagccg caggcaaac 
      tccgaaaat gttattatt tatgttttg gaccaaaca 
      tattaa
    mRNA sequence
    • >TC010410 RA
      tgtcttttc gacagtcaa agaaattag ttgtacttt 
      gacaacgct gctggcaaa tgtcacaaa aaagactga 
      tttaacctc actttttta ttgataaat ttgaaattc 
      tgttgtggt ttttgaaaa aatcagttt aattttgtt 
      gtctttgat tatataata ataaaatgg aaaattcgg 
      ccccttctc ttgccatca cgccaaacg tgttacttg 
      ccgaatgtt gcagtatct gtggaaaag cgcacagta 
      cccaaaatt gtgattacg tacaaattt tgaaatatg 
      tccatgata atgaaatac catccaggg ctaaactca 
      ctttaccca aatttttaa gcatacgaa ttctaagtg 
      atggctcat atgcaacaa ttaccaagt ctacaataa 
      gtaagggga ccagatttg gccctttcc aggccgaaa 
      aatccagta ctttggagc ctacgatga cttttcctt 
      tgaaaattt ttgataaag aaaatcgcg agtattttt 
      tagatgcca ccgacgaac agaagtgca gttggctga 
      tgtttgtgg ggcacgctg attgtgttg aagaacaga 
      atttaatct gctatcagg aaaaaggcg aaatttact 
      acgttgcaa ttaaggata ttaggattg gggagactc 
      ttaaggtgt ggtatgccc cctactatg ctcaaaaaa 
      tgaacaaag caatattag ccccaaaag tagccgacg 
      atcctttaa aaaatgtgg acattgatt ttttgatta 
      aaaagcaac aaaaaataa cggaacgta aagtctgga 
      cctgtaagt tttgtagtc acattgaga acaatatta 
      cttcgtttg cgaggcata tcttgaaac attacactg 
      ctcagcaca aaaacatgt gcgaattgt gtaagaaat 
      cgttcagat caaagaaag gcttaaata atcatttga 
      aaatcgtgc acaaaatgt tagtgactt cgtcaccca 
      aaacttcga aaagtgttg ttacgcccc cgaaaacag 
      attgcagag atgtggtac ttgggggtc ctttattga 
      acgatatgt tcacggaca gttccgatt tgcaacaat 
      ttgatctcc tagataatc agaatttat tattggaca 
      atgataatc taacagtgg aaaatctgc tcagtgaca 
      atgttaaag atttggacc atttcaatt tcgagatag 
      acgacagtc agaagcaat ttatttgtg atatttgtc 
      ttaagcaat ttaccaaaa tcaagctcc taatcgtgc 
      atttgaggc accatacag gggacttta tgtgttaca 
      agtgtctaa agatttttt gccgaaggg aaaatttaa 
      aatcgcaca tttgcaaca tgagcagtc accaccgct 
      gcgatttat gcgatcgta ctttcaccc aacgtaaat 
      acctctcgc ggcatctgg aagccgtcc ataataaaa 
      aatacagtt gcgtttcgt gtggcaaaa actatcact 
      cgaataagg agttgctgg aacacaact gtagcaaaa 
      tcccgaatg ccgacaagg agcgcttta tgtgcccca 
      tttgtgaca aactcttct tccgtgaaa tttatctca 
      agagacatt tgaaaactc acgaccaaa aaggcagaa 
      cttcaaagc cgaattacg taacttgcg acgtctgcg 
      gcttgcact taatcagca cttcgtatt tctcgcaca 
      agaagaaac accaagagc cttcatacg cttgcgaaa 
      tctgtagta aaaaattcc acaggaccg actcgctca 
      aacaacacg ttttgatcc atcaagcca acgagttcg 
      aatgtgata tttgccata aaaagtaca aaaccgaaa 
      aagtattta agtaccatc tcaagcggc ataatagtg 
      tgcaggcct acaagtgct cgctgtgcg acgcctcgt 
      tcaaaatcc gcaacagtt taaaccgac acattaaga 
      attttcaca gagaaaaaa gcaatgcaa agagcgaga 
      ttgaatact acatttgcc cagtttgcc aaaagtcaa 
      ttaaattgc gaagctcgc tgttgaggc atttgaaga 
      aatcgcacc ctgaagtgg ccaacattg acttgaacg 
      caattagac ccaaattat ttaaaaaca caaaaaata 
      atgataagg ataatgaag tgccgttta gtcttacga 
      acgaaatcg ataaccaaa ttaatacgt tgttgaaca 
      atgccgaaa ataacacaa cgattaata aaaacgccg 
      ttgatagtt tggtggaag agctggata ttaataaca 
      aaaatgagg acaaagaga cgagggaag tgtgtctct 
      cgattccgg accttaccg aaaattacc aggaaatca 
      ccttagata aaaacgctt atatcctgg ataatggca 
      ccattgttc agccgcagg caaactccg aaaatgtta 
      ttatttatg ttttggacc aaacatatt aagtttctc 
      gtttgattt aagtttaaa tgttgagtg tttgttgtt 
      aaatgaagt tttgtactt ataagcaaa aataaagac 
      agtttttca aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY