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Gene information
General information
TC010405 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 6616525..6648060
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010405
    iBeetle screen iB_01682
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010405 RA
      MATKPDYQR PYGIDGRYT EEPTQYSPA SHHEGAEPS 
      PHSRATTPL ECLPYTETD QENYPNVMR SEGDYNILF 
      NNTRIDEVP IDETSGTEY LQLGTVQNV SNGSPNGSA 
      NSNSGSPRR VAFTHLNSI QTSDQHTII GGSHLTQLT 
      SHISYTGDI ESTPGVNIA SSLYSRNPS SYTTGTMPY 
      YGTGSPELS SQSTQLWTN SGLNSNAGL VLAEEYPKV 
      TSSTALPAF NRLSTLHTN SHHRSAPYT VPSSSNYND 
      WQYGEPTGT LQYNILSPG ASRNRPLSA SASLSAMAA 
      EPGHGGVPD YYKNYYHGY NGANRAPAL HTTEEKSSR 
      RLSASRRVG LTCTNCHTS TTSLWRRNT VGEPVCNAC 
      GLYFKLHGV NRPLAMKKD SIQTRKRKP KGSKDSNSR 
      NALTNALES TINNIKLEQ SLPSVKLEH SSLENYNDL 
      RSISSLNHV SHNTNSNYV YNNQSHQRM SPYTSQSSQ 
      MTNSYFDML PQASPSSPS TTSPSPNSP LIVNNNNNN 
      NNTKVIMNG ENNNMERPT VVTLSS
    CDS sequence
    • >TC010405 RA
      atggcaaca aaaccagat tatcaacga ccatacggt 
      atcgatggc cgatacacc gaggagccc acgcaatac 
      agccccgcc tctcatcat gagggggct gagccttca 
      ccccactct agagcgaca acgcctctt gaatgtctg 
      ccctacaca gaaaccgat caggaaaat tatcccaat 
      gttatgcga tcggaaggc gattataac atccttttc 
      aataacacg cggatcgac gaggtgccc attgacgag 
      acctccggt acggagtac ctgcagctg ggtaccgtc 
      caaaatgtt tccaatgga tcgccgaac ggtagtgcg 
      aattctaac tcagggtcc cccaggaga gtggccttt 
      acacattta aattcgata cagacttca gaccagcac 
      accatcata ggggggtcg catctcacg caactgact 
      agccatatt agttacaca ggagatatc gaatccact 
      cctggagta aacatcgca agctctttg tactctcga 
      aatccgagc agttacacg actgggacc atgccttac 
      tacgggacg ggatcgccg gagttgtcc tcgcaatcg 
      acccaactc tggaccaac tcaggatta aattccaac 
      gctggactg gtgctagct gaagagtat ccaaaggtg 
      acttcctcg acggctctg ccggcattc aaccgcttg 
      tcgaccctt cacacgaac tctcaccac cgaagcgcc 
      ccgtatacg gtgccaagc tcgtcgaat tacaacgac 
      tggcagtac ggggagccc acggggacg ctccagtac 
      aacatcctg agtcctggg gcctcgcga aaccgcccc 
      ctgtcggcc tcagcctcc ctctcggca atggcggca 
      gagccaggc cacgggggt gttcctgat tactacaag 
      aactactac catgggtat aatggggcc aatagggcc 
      ccggctttg cacaccacc gaagaaaag tccagtcgc 
      cgtttgtct gcctctcgc cgagtgggt ttaacttgc 
      accaactgc cacacaagt acgacgtct ctttggcga 
      agaaacaca gttggtgag cctgtctgt aatgcttgc 
      ggcctttac ttcaagctc catggagtg aacaggcct 
      ttagcaatg aagaaggat agtatccag acaaggaaa 
      cggaaacct aaagggagc aaggacagc aactcccgg 
      aacgcccta actaacgcc ctagaatcc accattaac 
      aatatcaag ttggagcag agtctacct tctgttaaa 
      ttagaacat tcctcttta gagaattac aacgacttg 
      aggtctata tcgtcgctg aaccacgtg tcgcacaac 
      acaaactcg aattacgtg tacaacaat cagtctcat 
      caaaggatg tctccttac acgtcacag tcatcgcag 
      atgaccaac agttacttc gacatgctt ccgcaagcg 
      agtcctagt tcgccctcc acgacatca ccgagcccc 
      aattcgcca cttattgtc aacaataac aacaacaac 
      aacaacact aaagtgatt atgaacggg gaaaacaac 
      aacatggag agacccaca gtggtcact ttgtcctca 
      taa
    mRNA sequence
    • >TC010405 RA
      agaaacttg aatacatgg ctaggtatt tacataact 
      ccgctttga agaatgcaa aatgagcga tagaataag 
      aacaatttt aaaacaaaa atgtaattg tgatggatt 
      tacataccg gtggtgaac catattgag ctacaatga 
      ccacaattg agtaaaagt ccgagtcat aaaacgttt 
      accttatta cctgtgcaa actggcgtt actgttatc 
      gctgtgctg taatatcgt taatgttaa attttaatt 
      agaatgact tccttcaaa cgtcttatc gtgaaggtg 
      ggacgagtg ataaacgtg acctacata aacttacgt 
      gttgtagta ggtaatacc acatgagga aattttgaa 
      atcgaacac attttacta atttaagtt tttattgta 
      ctgtaaact ttgtaaggt aaacagaag tctccaagg 
      cttgtgagg cgtgtcgtg gattttgaa cttttgcct 
      cggccaata gaaatgagt aaacctgaa aaaacaagc 
      agaataata ccactattt gctctaaat gtaaaaata 
      gacaagatg gttatttct caattctta tctctgaac 
      tcaaatatt accatgagt attcaaatt gtgactaag 
      agcaaacca gccaaaggc aaagggata agcagattt 
      taatctctt tgttgttgt ttgggagag ggcggtttc 
      aataacaat tagtggatg atttacaat gtttttgca 
      ataagtaag agtctaaca agcaaatgt ccgtgtggt 
      aaacagctc acaacagca aacaaacaa catgttttt 
      cgaaatgaa atataattt attattcat ctcttgaac 
      cccgtttga acatcatct caactaatt ttcagattt 
      tattctaaa gtgaggtgg agatttgtt ttcaaggtt 
      tcttacaat ttgacccag tcagatgtc aaagttcac 
      gtgataaca ttagtgaac ttacaccat ccctcgttt 
      ttcttctta ttttacgat ggtgaaata gatcttttt 
      gaaagacta atgaaagcg tagaaaata ttttttttc 
      tttttggga tcaaaccgt aaaacgccg ccgtcaacg 
      cgcgcggaa agatatcta aaatttacg gccgcagac 
      ggcatttat cagggcgaa agctgtaac aaaactaaa 
      taaaccctg aaataaagg atgtgaaaa ttcattttt 
      tccaagaaa gtgtaaaac tgatggcat atgcattct 
      tatggtgta aataaagga ccaacaaaa aaatttcgg 
      gtgaaatga gccagaaaa tattgataa aatatgcaa 
      atgggtgag aaattgaaa gagtagaga agacccaat 
      gatacttac gcttgtcca aaataagaa acaaataaa 
      attgaggcg tttctcccc acttcccac caataacac 
      aagcacgaa ccaatcccc actacaaaa ttgtggccg 
      ctgaaaaat taaaactgt ccgaccgct ccgtcgttc 
      ttgccatga ctgttgtac tcgagtgtt ataaacgtt 
      gcagttggg tttgttgat agtggttga ttttgataa 
      caccgatat ggcaacaaa accagatta tcaacgacc 
      atacggtat cgatggccg atacaccga ggagcccac 
      gcaatacag ccccgcctc tcatcatga gggggctga 
      gccttcacc ccactctag agcgacaac gcctcttga 
      atgtctgcc ctacacaga aaccgatca ggaaaatta 
      tcccaatgt tatgcgatc ggaaggcga ttataacat 
      ccttttcaa taacacgcg gatcgacga ggtgcccat 
      tgacgagac ctccggtac ggagtacct gcagctggg 
      taccgtcca aaatgtttc caatggatc gccgaacgg 
      tagtgcgaa ttctaactc agggtcccc caggagagt 
      ggcctttac acatttaaa ttcgataca gacttcaga 
      ccagcacac catcatagg ggggtcgca tctcacgca 
      actgactag ccatattag ttacacagg agatatcga 
      atccactcc tggagtaaa catcgcaag ctctttgta 
      ctctcgaaa tccgagcag ttacacgac tgggaccat 
      gccttacta cgggacggg atcgccgga gttgtcctc 
      gcaatcgac ccaactctg gaccaactc aggattaaa 
      ttccaacgc tggactggt gctagctga agagtatcc 
      aaaggtgac ttcctcgac ggctctgcc ggcattcaa 
      ccgcttgtc gacccttca cacgaactc tcaccaccg 
      aagcgcccc gtatacggt gccaagctc gtcgaatta 
      caacgactg gcagtacgg ggagcccac ggggacgct 
      ccagtacaa catcctgag tcctggggc ctcgcgaaa 
      ccgccccct gtcggcctc agcctccct ctcggcaat 
      ggcggcaga gccaggcca cgggggtgt tcctgatta 
      ctacaagaa ctactacca tgggtataa tggggccaa 
      tagggcccc ggctttgca caccaccga agaaaagtc 
      cagtcgccg tttgtctgc ctctcgccg agtgggttt 
      aacttgcac caactgcca cacaagtac gacgtctct 
      ttggcgaag aaacacagt tggtgagcc tgtctgtaa 
      tgcttgcgg cctttactt caagctcca tggagtgaa 
      caggccttt agcaatgaa gaaggatag tatccagac 
      aaggaaacg gaaacctaa agggagcaa ggacagcaa 
      ctcccggaa cgccctaac taacgccct agaatccac 
      cattaacaa tatcaagtt ggagcagag tctaccttc 
      tgttaaatt agaacattc ctctttaga gaattacaa 
      cgacttgag gtctatatc gtcgctgaa ccacgtgtc 
      gcacaacac aaactcgaa ttacgtgta caacaatca 
      gtctcatca aaggatgtc tccttacac gtcacagtc 
      atcgcagat gaccaacag ttacttcga catgcttcc 
      gcaagcgag tcctagttc gccctccac gacatcacc 
      gagccccaa ttcgccact tattgtcaa caataacaa 
      caacaacaa caacactaa agtgattat gaacgggga 
      aaacaacaa catggagag acccacagt ggtcacttt 
      gtcctcata attgttctc tgaaatacc gtatcatta 
      gtggacgta taagaatac ataattgca ataatataa 
      atatgctta tggttactt taccgataa gttgtgttt 
      aggtattta acgagtgtc gtctgttgt cctatcttt 
      tattggaag agatctcta atttgttat tgtttgacc 
      tgttgatcg caaattcgt aattgtgaa aaggttatt 
      ttcggctgt aataacatg agaatgcaa cttgtgtaa 
      catgacagt ctagaaagt aaatatttt tgttaaata 
      gctttatcc ataagatta tcgaaattt aataacacg 
      ttgtgatag ggaaatgga tattttgcg gacatttat 
      tgagaagga agttgagta caaaggtag tttgttatt 
      tgtatatta tatatactt aaaagaaaa tttctctga 
      aattaccaa agacaattg tagtttttt caacactag 
      gtttaatat tctcgaata tcactttgc ttttaagag 
      ttaattgtt ttattttga ctgattgaa tctacacaa 
      aacatttat ttgaaaaag gtctgatgt acattgtct 
      agatctttt tattaagag tgtttattc taatcattt 
      agaagttaa ggatttttg ttttattga ttatatttt 
      gttgtatat taatatctc tcttattta ttgtgagtt 
      attgaattg tgaatccac attccgagt acttgtaca 
      gttcgttta cttgtaagg tttaggttc tgtagatat 
      tttgcaaag ttagatgca aacacaaaa taaattttc 
      tccaagtta cttttaatt tttgtataa tcacatttt 
      taaatttta caaatatta ttggttatt tactgatat 
      tattatttt gtactgctt gtatattat ttcataata 
      aaataattt aatcttt