Status
Please wait ...
Gene information
General information
TC010395 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7890967..7892693
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC010395
      iBeetle screen iB_01680

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      larva cuticle dorsal not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      head bristle irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      maxilla sometimes not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

      eclosion delayed (still pupa) - Number of animals showing the phenotype: 6 (16 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 5 (22 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
      Days post injection


      Pupal morphology
      dorsal thoracic muscles irregular - Number of animals showing the phenotype: 3

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC010395 RA
        MSSDVSQDE YEYVQYNTE FYEEEEGPP LIKKRSRLI 
        CAVVQNNVD LLRDLLDSP KWYTVEDSM GYNLLQIAV 
        LQERREVFD YMLSIPDFP FGALNRDGE TALDVAVTC 
        FVSNDIWID GYFARELIK KGVFTTRHE FLLQNAIER 
        SLTEVAKAL IEKGVDVNK VFDEEWGHT LLQTAVGEN 
        NAELTAMLL IFGADPFVL YSTGYTVFE RSSPELQEI 
        LFYYIYDQY SDAEIHLEE LLSLATTNP FLFNEIFKN 
        SIDITINNC NPCTCFEIL ALMDIESLK LTVSKCEHY 
        LKQMFSVPF RRKTQVRNS QWPLETSFF LLETDLKDE 
        IVNVINSLD SSSLLQNMI RDEKNESEI SEVLCFLLS 
        YGLEIVAYD LHLIYSKFG YCNLYKILL HMDISGKCD 
        DFNNVKIMP LFVYDVTIE NCLGFLESS EPSHLLELS 
        SYFCHQNLT EKCERAIAD GHLEKRTFQ QIPLLVELA 
        RNTFRKFFI EKFQIKTSR RFYSLLNCL PISATHRKI 
        ISYETPLYC
      CDS sequence
      • >TC010395 RA
        atgtcttca gacgtgtcc caagacgag tacgagtac 
        gtgcagtac aataccgag ttctatgaa gaggaggag 
        ggcccccct ctgatcaaa aaaaggtcg cgtttaatt 
        tgtgcagtc gtccaaaac aatgtggac ctccttcga 
        gatttgctg gactctcct aagtggtac actgtggag 
        gacagcatg gggtacaac ttgctacaa attgcagtt 
        ttgcaagag agacgggaa gtgtttgac tacatgtta 
        tcaattcct gacttcccc ttcggtgcc ttaaatcgt 
        gatggagag acggctcta gacgttgcg gttacatgt 
        tttgtgagt aacgatatt tggattgac ggatacttc 
        gcccgtgag ttaattaaa aagggggtt ttcacaaca 
        cggcacgaa ttcttatta caaaacgct attgaacga 
        tctcttact gaagttgcg aaggcgtta atcgagaaa 
        ggagttgat gttaacaaa gttttcgat gaggaatgg 
        gggcacaca cttttgcaa actgccgtt ggggaaaat 
        aatgctgag ctaactgca atgttatta atatttggt 
        gccgacccg ttcgtcttg tattctaca gggtacacc 
        gttttcgaa agaagtagt ccagaatta caagaaatc 
        ttattttac tacatatac gatcaatat tcagatgca 
        gaaatccac ttggaagag ttgttatcg cttgccacg 
        acgaatccg tttttattt aacgaaatt ttcaaaaat 
        tcaattgat atcaccatt aataattgt aatccttgt 
        acttgtttt gaaatcttg gcccttatg gacatcgaa 
        tctttgaaa ttaactgtg tcaaagtgt gagcattac 
        ttgaaacaa atgttttcg gtgccgttt cggagaaaa 
        acacaagta cgtaatagc caatggcct ttagaaact 
        tcatttttc ttacttgag actgacttg aaagacgaa 
        atcgtcaat gttattaac tcattagat agttcctca 
        cttttacaa aatatgatt agagatgaa aaaaacgaa 
        tctgaaatt agcgaagtt ctttgtttt ttgttgagc 
        tacggcctc gagattgtt gcatacgac cttcatttg 
        atttactcc aagtttggg tattgcaat ttatacaaa 
        attcttcta cacatggat atttcaggg aaatgtgat 
        gattttaac aatgtgaaa attatgccg ttatttgtt 
        tatgatgtg accattgaa aattgtcta ggttttttg 
        gaatcaagc gaaccctcc catttactt gaactttct 
        tcgtatttt tgtcaccag aacttgact gaaaaatgt 
        gaacgtgca attgctgat ggacactta gaaaaacga 
        acgttccag caaatacct ctactggtt gagttggcg 
        agaaatacg tttcgaaag ttttttatc gaaaaattt 
        caaatcaaa acgtctcgg aggttctat tcgcttttg 
        aactgctta cccataagt gctactcat aggaagatt 
        attagttat gaaacacca ctgtattgt taa
      mRNA sequence
      • >TC010395 RA
        gatatcgta attctgggg attaagtcc ttcagagtc 
        ataattccc agtcaaaat gtcttcaga cgtgtccca 
        agacgagta cgagtacgt gcagtacaa taccgagtt 
        ctatgaaga ggaggaggg cccccctct gatcaaaaa 
        aaggtcgcg tttaatttg tgcagtcgt ccaaaacaa 
        tgtggacct ccttcgaga tttgctgga ctctcctaa 
        gtggtacac tgtggagga cagcatggg gtacaactt 
        gctacaaat tgcagtttt gcaagagag acgggaagt 
        gtttgacta catgttatc aattcctga cttcccctt 
        cggtgcctt aaatcgtga tggagagac ggctctaga 
        cgttgcggt tacatgttt tgtgagtaa cgatatttg 
        gattgacgg atacttcgc ccgtgagtt aattaaaaa 
        gggggtttt cacaacacg gcacgaatt cttattaca 
        aaacgctat tgaacgatc tcttactga agttgcgaa 
        ggcgttaat cgagaaagg agttgatgt taacaaagt 
        tttcgatga ggaatgggg gcacacact tttgcaaac 
        tgccgttgg ggaaaataa tgctgagct aactgcaat 
        gttattaat atttggtgc cgacccgtt cgtcttgta 
        ttctacagg gtacaccgt tttcgaaag aagtagtcc 
        agaattaca agaaatctt attttacta catatacga 
        tcaatattc agatgcaga aatccactt ggaagagtt 
        gttatcgct tgccacgac gaatccgtt tttatttaa 
        cgaaatttt caaaaattc aattgatat caccattaa 
        taattgtaa tccttgtac ttgttttga aatcttggc 
        ccttatgga catcgaatc tttgaaatt aactgtgtc 
        aaagtgtga gcattactt gaaacaaat gttttcggt 
        gccgtttcg gagaaaaac acaagtacg taatagcca 
        atggccttt agaaacttc atttttctt acttgagac 
        tgacttgaa agacgaaat cgtcaatgt tattaactc 
        attagatag ttcctcact tttacaaaa tatgattag 
        agatgaaaa aaacgaatc tgaaattag cgaagttct 
        ttgtttttt gttgagcta cggcctcga gattgttgc 
        atacgacct tcatttgat ttactccaa gtttgggta 
        ttgcaattt atacaaaat tcttctaca catggatat 
        ttcagggaa atgtgatga ttttaacaa tgtgaaaat 
        tatgccgtt atttgttta tgatgtgac cattgaaaa 
        ttgtctagg ttttttgga atcaagcga accctccca 
        tttacttga actttcttc gtatttttg tcaccagaa 
        cttgactga aaaatgtga acgtgcaat tgctgatgg 
        acacttaga aaaacgaac gttccagca aatacctct 
        actggttga gttggcgag aaatacgtt tcgaaagtt 
        ttttatcga aaaatttca aatcaaaac gtctcggag 
        gttctattc gcttttgaa ctgcttacc cataagtgc 
        tactcatag gaagattat tagttatga aacaccact 
        gtattgtta aactatttt tgtatgttg atattgccg 
        aataaatta tttacttgt aa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY