Status
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Gene information
General information
TC032596
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010384,TC010383
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid24: 955016..968205
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032596
    iBeetle screen iB_01678
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032596 RA
      MQLRRVPSK TKIKLTNSK KSCKKRKQQ NCRKNDKCV 
      CMPMQLSTF EFPRIWKEL RLKNQLCDG IVRCADGTE 
      FKIHRAILS AASPYFRAL FTNSINRGQ PETYEANVS 
      IPGKIFALL LDFAYTGTC SINSTNVEN LLKFADRYE 
      ILGVVQMCC RYLLDELSP TNCLGILHF ANQYFCSDL 
      VKRGQRYVK HNFAKVLGE SSEFVHLDK NELKEIISD 
      DELNIKHEE IVFDAIMKW INFCPNSRK IHFFELLRC 
      VRFGNMLRE NILIISNHQ FVEEKSECK EYMEKVVAQ 
      TELAAYPGK IDVNNILFR PRISYDVLF AIGGWSAGS 
      PTNFVETYD IRADRWLLS SDTDSFPRA YHGLCTLNG 
      IIYVIGGFD GNQYFNTVR RFDPVNHTW SECACMYHH 
      RCYVSVVMA DNMIYAMGG YNGRSRMNT AEKYDPSKN 
      QWEMIPPMQ KQRSDASAA TLNEKIYIV GGFNGQEVM 
      RSAEVFDIK TNQWSYIPQ MISARSGVS LVVYDNTLY 
      ALGGFNGYV RLTSGEKYV PGESPWWTE ISEMMTPRS 
      NFATVILDD YIYVIGGFN DNFVSGSST INFVEYYDP 
      EADDWYDAS PMNLNRSAL SACVISGLP NTKDYSILG 
      RSQQEWGQG AEIGSN
    • >TC032596 RB
      MQLRRVPSK TKIKLTNSK KSCKKRKQQ NCRKNDKCV 
      CMPMQLSTF EFPRIWKEL RLKNQLCDG IVRCADGTE 
      FKIHRAILS AASPYFRAL FTNSINRGQ PETYEANVS 
      IPGKIFALL LDFAYTGTC SINSTNVEN LLKFADRYE 
      ILGVVQMCC RYLLDELSP TNCLGILHF ANQYFCSDL 
      VKRGQRYVK HNFAKVLGE SSEFVHLDK NELKEIISD 
      DELNIKHEE IVFDAIMKW INFCPNSRK IHFFELLRC 
      VRFGNMLRE NILIISNHQ FVEEKSECK EYMEKVVAQ 
      TELAAYPGK IDVNNILFR PRISYDVLF AIGGWSAGS 
      PTNFVETYD IRADRWLLS SDTDSFPRA YHGLCTLNG 
      IIYVIGGFD GNQYFNTVR RFDPVNHTW SECACMYHH 
      RCYVSVVMA DNMIYAMGG YNGRSRMNT AEKYDPSKN 
      QWEMIPPMQ KQRSDASAA TLNEKIYIV GGFNGQEVM 
      RSAEVFDIK TNQWSYIPQ MISARSGVS LVVYDNTLY 
      ALGGFNGYV RLTSGEKYV PGESPWWTE ISEMMTPRS 
      NFATVILDD YIYVIGGFN GSSTINFVE YYDPEADDW 
      YDASPMNLN RSALSACVI SGLPNTKDY SILGRSQQE 
      WGQGAEIGS N
    CDS sequence
    • >TC032596 RA
      atgcagctt cgacgcgtc ccctcaaaa acaaaaata 
      aagttgaca aattcgaaa aaatcatgc aagaagcga 
      aaacaacag aattgcaga aagaacgac aagtgtgtg 
      tgtatgccg atgcagctt agcaccttc gaatttccc 
      cgaatttgg aaagaattg cgtctcaaa aaccaactt 
      tgcgacggg atcgtccgt tgtgccgac ggcaccgag 
      ttcaaaatc caccgagcc atcctctcg gcagccagt 
      ccctacttc cgagccctc ttcaccaac tcgatcaat 
      cggggccag cccgagacc tacgaggcc aatgtgagc 
      attccgggc aaaatcttt gccctgctg ctcgatttc 
      gcctacacg gggacgtgc tcgatcaac tccacgaac 
      gtggagaat ttgctcaag tttgccgac cggtacgag 
      atcctcgga gttgtgcaa atgtgctgc aggtatttg 
      ctggatgaa ctaagtccg acgaattgc ttgggaatt 
      ttgcacttt gccaatcag tatttttgc agcgatttg 
      gtgaagcga gggcaaaga tacgtcaag cacaacttt 
      gccaaagtt ttgggcgaa agtagcgaa tttgtccat 
      ttggacaaa aacgaactg aaggaaatt ataagtgac 
      gatgaattg aatatcaaa cacgaagaa attgtattt 
      gatgcaatt atgaaatgg atcaatttt tgcccaaac 
      agtcgcaag attcatttt tttgagctg ctgaggtgt 
      gtcagattt gggaacatg ttaagggag aatattttg 
      ataatatcc aatcaccag tttgtcgaa gaaaagtct 
      gaatgtaag gaatacatg gagaaagtt gtggctcag 
      actgaactt gctgcctat cccggaaaa attgacgtt 
      aacaatatt ttatttcgg ccaaggatt tcctacgat 
      gtgcttttt gcaatcggg ggttggagt gctggaagt 
      ccgacgaat tttgtcgaa acttatgat atccgagct 
      gatcgttgg ctactttct tctgacact gattctttt 
      ccaagagct tatcatggg ttgtgtact ttgaatggg 
      attatttat gtgattgga ggctttgat ggtaaccag 
      tattttaac acagttcgg cgttttgat ccagtgaac 
      cacacctgg agtgagtgt gcttgtatg taccaccat 
      cgttgttat gtgagtgtt gtaatggcc gataatatg 
      atatatgcc atgggaggg tacaatggt agaagcaga 
      atgaatacg gccgagaaa tatgatcct agcaaaaac 
      caatgggag atgattcct ccaatgcag aaacaacgg 
      tctgatgcc agtgctgcc acgcttaac gaaaaaatt 
      tacatagtt gggggcttt aacggccag gaagtgatg 
      cgatccgcc gaagtcttc gatatcaaa accaaccaa 
      tggtcttac atcccccaa atgatatcg gcgcgttcc 
      ggcgtctct ttggtggtt tacgacaac accctgtat 
      gcactgggg ggtttcaac ggctacgtg agactcaca 
      agtggcgag aaatacgtc cctggcgag tctccctgg 
      tggacggaa atctccgaa atgatgaca cctcgcagt 
      aattttgcc acagttatc ctagatgac tacatctac 
      gtcatcgga gggttcaac gacaacttc gtgtcaggt 
      tcgtcgact atcaacttc gtggagtat tacgatccg 
      gaagcggat gactggtat gatgcctca ccgatgaat 
      ttgaacaga tcggctttg agtgcttgc gttattagt 
      ggattgccg aatactaaa gattattcg atactgggg 
      cggagtcaa caggaatgg ggacaagga gctgagatt 
      ggctcaaat taa
    • >TC032596 RB
      atgcagctt cgacgcgtc ccctcaaaa acaaaaata 
      aagttgaca aattcgaaa aaatcatgc aagaagcga 
      aaacaacag aattgcaga aagaacgac aagtgtgtg 
      tgtatgccg atgcagctt agcaccttc gaatttccc 
      cgaatttgg aaagaattg cgtctcaaa aaccaactt 
      tgcgacggg atcgtccgt tgtgccgac ggcaccgag 
      ttcaaaatc caccgagcc atcctctcg gcagccagt 
      ccctacttc cgagccctc ttcaccaac tcgatcaat 
      cggggccag cccgagacc tacgaggcc aatgtgagc 
      attccgggc aaaatcttt gccctgctg ctcgatttc 
      gcctacacg gggacgtgc tcgatcaac tccacgaac 
      gtggagaat ttgctcaag tttgccgac cggtacgag 
      atcctcgga gttgtgcaa atgtgctgc aggtatttg 
      ctggatgaa ctaagtccg acgaattgc ttgggaatt 
      ttgcacttt gccaatcag tatttttgc agcgatttg 
      gtgaagcga gggcaaaga tacgtcaag cacaacttt 
      gccaaagtt ttgggcgaa agtagcgaa tttgtccat 
      ttggacaaa aacgaactg aaggaaatt ataagtgac 
      gatgaattg aatatcaaa cacgaagaa attgtattt 
      gatgcaatt atgaaatgg atcaatttt tgcccaaac 
      agtcgcaag attcatttt tttgagctg ctgaggtgt 
      gtcagattt gggaacatg ttaagggag aatattttg 
      ataatatcc aatcaccag tttgtcgaa gaaaagtct 
      gaatgtaag gaatacatg gagaaagtt gtggctcag 
      actgaactt gctgcctat cccggaaaa attgacgtt 
      aacaatatt ttatttcgg ccaaggatt tcctacgat 
      gtgcttttt gcaatcggg ggttggagt gctggaagt 
      ccgacgaat tttgtcgaa acttatgat atccgagct 
      gatcgttgg ctactttct tctgacact gattctttt 
      ccaagagct tatcatggg ttgtgtact ttgaatggg 
      attatttat gtgattgga ggctttgat ggtaaccag 
      tattttaac acagttcgg cgttttgat ccagtgaac 
      cacacctgg agtgagtgt gcttgtatg taccaccat 
      cgttgttat gtgagtgtt gtaatggcc gataatatg 
      atatatgcc atgggaggg tacaatggt agaagcaga 
      atgaatacg gccgagaaa tatgatcct agcaaaaac 
      caatgggag atgattcct ccaatgcag aaacaacgg 
      tctgatgcc agtgctgcc acgcttaac gaaaaaatt 
      tacatagtt gggggcttt aacggccag gaagtgatg 
      cgatccgcc gaagtcttc gatatcaaa accaaccaa 
      tggtcttac atcccccaa atgatatcg gcgcgttcc 
      ggcgtctct ttggtggtt tacgacaac accctgtat 
      gcactgggg ggtttcaac ggctacgtg agactcaca 
      agtggcgag aaatacgtc cctggcgag tctccctgg 
      tggacggaa atctccgaa atgatgaca cctcgcagt 
      aattttgcc acagttatc ctagatgac tacatctac 
      gtcatcgga gggttcaac ggttcgtcg actatcaac 
      ttcgtggag tattacgat ccggaagcg gatgactgg 
      tatgatgcc tcaccgatg aatttgaac agatcggct 
      ttgagtgct tgcgttatt agtggattg ccgaatact 
      aaagattat tcgatactg gggcggagt caacaggaa 
      tggggacaa ggagctgag attggctca aattaa
    mRNA sequence
    • >TC032596 RA
      acgtttgat taaaaacct caaaataat aaagtggct 
      ttgaaataa attgaagtg tccactaaa tgtcacatt 
      aatcaattt cgaaaatat acacacact tcccaagcc 
      agtgaacca tcacattca aatggattt tacgatgca 
      gcttcgacg cgtcccctc aaaaacaaa aataaagtt 
      gacaaattc gaaaaaatc atgcaagaa gcgaaaaca 
      acagaattg cagaaagaa cgacaagtg tgtgtgtat 
      gccgatgca gcttagcac cttcgaatt tccccgaat 
      ttggaaaga attgcgtct caaaaacca actttgcga 
      cgggatcgt ccgttgtgc cgacggcac cgagttcaa 
      aatccaccg agccatcct ctcggcagc cagtcccta 
      cttccgagc cctcttcac caactcgat caatcgggg 
      ccagcccga gacctacga ggccaatgt gagcattcc 
      gggcaaaat ctttgccct gctgctcga tttcgccta 
      cacggggac gtgctcgat caactccac gaacgtgga 
      gaatttgct caagtttgc cgaccggta cgagatcct 
      cggagttgt gcaaatgtg ctgcaggta tttgctgga 
      tgaactaag tccgacgaa ttgcttggg aattttgca 
      ctttgccaa tcagtattt ttgcagcga tttggtgaa 
      gcgagggca aagatacgt caagcacaa ctttgccaa 
      agttttggg cgaaagtag cgaatttgt ccatttgga 
      caaaaacga actgaagga aattataag tgacgatga 
      attgaatat caaacacga agaaattgt atttgatgc 
      aattatgaa atggatcaa tttttgccc aaacagtcg 
      caagattca tttttttga gctgctgag gtgtgtcag 
      atttgggaa catgttaag ggagaatat tttgataat 
      atccaatca ccagtttgt cgaagaaaa gtctgaatg 
      taaggaata catggagaa agttgtggc tcagactga 
      acttgctgc ctatcccgg aaaaattga cgttaacaa 
      tattttatt tcggccaag gatttccta cgatgtgct 
      ttttgcaat cgggggttg gagtgctgg aagtccgac 
      gaattttgt cgaaactta tgatatccg agctgatcg 
      ttggctact ttcttctga cactgattc ttttccaag 
      agcttatca tgggttgtg tactttgaa tgggattat 
      ttatgtgat tggaggctt tgatggtaa ccagtattt 
      taacacagt tcggcgttt tgatccagt gaaccacac 
      ctggagtga gtgtgcttg tatgtacca ccatcgttg 
      ttatgtgag tgttgtaat ggccgataa tatgatata 
      tgccatggg agggtacaa tggtagaag cagaatgaa 
      tacggccga gaaatatga tcctagcaa aaaccaatg 
      ggagatgat tcctccaat gcagaaaca acggtctga 
      tgccagtgc tgccacgct taacgaaaa aatttacat 
      agttggggg ctttaacgg ccaggaagt gatgcgatc 
      cgccgaagt cttcgatat caaaaccaa ccaatggtc 
      ttacatccc ccaaatgat atcggcgcg ttccggcgt 
      ctctttggt ggtttacga caacaccct gtatgcact 
      ggggggttt caacggcta cgtgagact cacaagtgg 
      cgagaaata cgtccctgg cgagtctcc ctggtggac 
      ggaaatctc cgaaatgat gacacctcg cagtaattt 
      tgccacagt tatcctaga tgactacat ctacgtcat 
      cggagggtt caacgacaa cttcgtgtc aggttcgtc 
      gactatcaa cttcgtgga gtattacga tccggaagc 
      ggatgactg gtatgatgc ctcaccgat gaatttgaa 
      cagatcggc tttgagtgc ttgcgttat tagtggatt 
      gccgaatac taaagatta ttcgatact ggggcggag 
      tcaacagga atggggaca aggagctga gattggctc 
      aaattaatg tgttcgaag agtgaaaat gtgtggttc 
      ttgcctttt ttatctata cttgtttat tttttaaat 
      ttgccagtt ttatacaat aaaatatta cattttga
    • >TC032596 RB
      acgtttgat taaaaacct caaaataat aaagtggct 
      ttgaaataa attgaagtg tccactaaa tgtcacatt 
      aatcaattt cgaaaatat acacacact tcccaagcc 
      agtgaacca tcacattca aatggattt tacgatgca 
      gcttcgacg cgtcccctc aaaaacaaa aataaagtt 
      gacaaattc gaaaaaatc atgcaagaa gcgaaaaca 
      acagaattg cagaaagaa cgacaagtg tgtgtgtat 
      gccgatgca gcttagcac cttcgaatt tccccgaat 
      ttggaaaga attgcgtct caaaaacca actttgcga 
      cgggatcgt ccgttgtgc cgacggcac cgagttcaa 
      aatccaccg agccatcct ctcggcagc cagtcccta 
      cttccgagc cctcttcac caactcgat caatcgggg 
      ccagcccga gacctacga ggccaatgt gagcattcc 
      gggcaaaat ctttgccct gctgctcga tttcgccta 
      cacggggac gtgctcgat caactccac gaacgtgga 
      gaatttgct caagtttgc cgaccggta cgagatcct 
      cggagttgt gcaaatgtg ctgcaggta tttgctgga 
      tgaactaag tccgacgaa ttgcttggg aattttgca 
      ctttgccaa tcagtattt ttgcagcga tttggtgaa 
      gcgagggca aagatacgt caagcacaa ctttgccaa 
      agttttggg cgaaagtag cgaatttgt ccatttgga 
      caaaaacga actgaagga aattataag tgacgatga 
      attgaatat caaacacga agaaattgt atttgatgc 
      aattatgaa atggatcaa tttttgccc aaacagtcg 
      caagattca tttttttga gctgctgag gtgtgtcag 
      atttgggaa catgttaag ggagaatat tttgataat 
      atccaatca ccagtttgt cgaagaaaa gtctgaatg 
      taaggaata catggagaa agttgtggc tcagactga 
      acttgctgc ctatcccgg aaaaattga cgttaacaa 
      tattttatt tcggccaag gatttccta cgatgtgct 
      ttttgcaat cgggggttg gagtgctgg aagtccgac 
      gaattttgt cgaaactta tgatatccg agctgatcg 
      ttggctact ttcttctga cactgattc ttttccaag 
      agcttatca tgggttgtg tactttgaa tgggattat 
      ttatgtgat tggaggctt tgatggtaa ccagtattt 
      taacacagt tcggcgttt tgatccagt gaaccacac 
      ctggagtga gtgtgcttg tatgtacca ccatcgttg 
      ttatgtgag tgttgtaat ggccgataa tatgatata 
      tgccatggg agggtacaa tggtagaag cagaatgaa 
      tacggccga gaaatatga tcctagcaa aaaccaatg 
      ggagatgat tcctccaat gcagaaaca acggtctga 
      tgccagtgc tgccacgct taacgaaaa aatttacat 
      agttggggg ctttaacgg ccaggaagt gatgcgatc 
      cgccgaagt cttcgatat caaaaccaa ccaatggtc 
      ttacatccc ccaaatgat atcggcgcg ttccggcgt 
      ctctttggt ggtttacga caacaccct gtatgcact 
      ggggggttt caacggcta cgtgagact cacaagtgg 
      cgagaaata cgtccctgg cgagtctcc ctggtggac 
      ggaaatctc cgaaatgat gacacctcg cagtaattt 
      tgccacagt tatcctaga tgactacat ctacgtcat 
      cggagggtt caacggttc gtcgactat caacttcgt 
      ggagtatta cgatccgga agcggatga ctggtatga 
      tgcctcacc gatgaattt gaacagatc ggctttgag 
      tgcttgcgt tattagtgg attgccgaa tactaaaga 
      ttattcgat actggggcg gagtcaaca ggaatgggg 
      acaaggagc tgagattgg ctcaaatta atgtgttcg 
      aagagtgaa aatgtgtgg ttcttgcct tttttatct 
      atacttgtt tatttttta aatttgcca gttttatac 
      aataaaata ttacatttt ga