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Gene information
General information
TC010373 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid24: 560271..566534
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC010373
      iBeetle screen iB_01677
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      Comment: larvae with reduced fluorescence were still moving, so the decreasing fluorescence isprobably not to the death of the larvae
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC010373 RA
        MELVKDLVN NQLTLYLLP VALVIIVVI LVFTFGFKS 
        AEQPPFDKL TNDDRKQAG KKKKIKEKK TTANGHVST 
        GDAKTEKSP VKDSKRSPT KDSTDSQKP KDKKIENNK 
        QAEKNKKNE ANKNKVIEE VKNKKNLKK FLSEKPVDF 
        DDGNWETVP SKADKKKKP DQSPAKKDK KSKKVEKAA 
        EIEKLVKEV VNKELREKE KEIKVEKPV EEPIQIEEP 
        VVVIEEKKE KKKEKKAKK ENEKPVEKE PPVPKEVPK 
        QKVEKVIEE IREEPPKTP VKEGGVAFD ELGDVWTEA 
        KVAKKSKKK ARRDN
      CDS sequence
      • >TC010373 RA
        atggaatta gtgaaagat cttgtaaat aaccaactc 
        accctctac ctgttacct gtggctttg gtcattatt 
        gtagtgatt ctagtgttc acattcgga ttcaaatcg 
        gcagaacaa ccacctttc gacaaactc accaacgat 
        gatcgaaaa caagcaggg aaaaagaag aaaatcaag 
        gaaaagaaa accaccgcc aacggacac gtgtctact 
        ggtgatgca aaaaccgag aaatcacca gtcaaagat 
        tcaaaaagg tcgcctact aaagattcg accgactct 
        caaaaacca aaagacaag aaaatagaa aacaacaaa 
        caggccgaa aagaataag aagaacgaa gccaacaaa 
        aataaagtg atcgaagaa gtgaaaaac aagaagaac 
        ttgaagaaa ttcctcagt gagaaacca gtggatttc 
        gacgatggc aactgggag accgttccc agcaaagcc 
        gataagaag aagaaaccc gatcagagt ccagccaag 
        aaggacaag aagtcgaaa aaggtcgag aaagcggcc 
        gaaattgaa aagttagtc aaggaagtg gttaacaag 
        gagctgcgt gagaaggaa aaggagatc aaggttgag 
        aaacctgta gaggaaccg attcagatt gaggagcct 
        gttgtcgtg attgaagag aagaaagaa aagaagaag 
        gaaaagaag gccaagaag gagaatgaa aaaccggtt 
        gagaaagaa ccgccggtt ccaaaggaa gttcctaaa 
        cagaaggtt gagaaagtg attgaagag attagggaa 
        gaaccgccc aagactccc gtcaaagag ggaggcgtt 
        gccttcgac gagctggga gatgtttgg acagaagcg 
        aaggtggcg aagaagagt aagaaaaaa gcgcgtaga 
        gataactag
      mRNA sequence
      • >TC010373 RA
        agactaaaa aatcagtaa gtcaattcc aataattac 
        aataatttg cttttttcg agtcttttg aaatcatta 
        attttgcaa aaaactagt caaaaaaac caccgtagt 
        agtgttggc agtgtagaa ttccccaat ttgtcccta 
        attttgaca gcttgcgca aatacgtgg cgcagaatg 
        cagaaaata aacatatgt gtctgttta cgtcacagc 
        cgcgtcacc aaaaattag atttgcggt ggttcgttg 
        gttccgtgg cagcttttc ggtagaaat aatcacctt 
        tcggtccat caaaatgga attagtgaa agatcttgt 
        aaataacca actcaccct ctacctgtt acctgtggc 
        tttggtcat tattgtagt gattctagt gttcacatt 
        cggattcaa atcggcaga acaaccacc tttcgacaa 
        actcaccaa cgatgatcg aaaacaagc agggaaaaa 
        gaagaaaat caaggaaaa gaaaaccac cgccaacgg 
        acacgtgtc tactggtga tgcaaaaac cgagaaatc 
        accagtcaa agattcaaa aaggtcgcc tactaaaga 
        ttcgaccga ctctcaaaa accaaaaga caagaaaat 
        agaaaacaa caaacaggc cgaaaagaa taagaagaa 
        cgaagccaa caaaaataa agtgatcga agaagtgaa 
        aaacaagaa gaacttgaa gaaattcct cagtgagaa 
        accagtgga tttcgacga tggcaactg ggagaccgt 
        tcccagcaa agccgataa gaagaagaa acccgatca 
        gagtccagc caagaagga caagaagtc gaaaaaggt 
        cgagaaagc ggccgaaat tgaaaagtt agtcaagga 
        agtggttaa caaggagct gcgtgagaa ggaaaagga 
        gatcaaggt tgagaaacc tgtagagga accgattca 
        gattgagga gcctgttgt cgtgattga agagaagaa 
        agaaaagaa gaaggaaaa gaaggccaa gaaggagaa 
        tgaaaaacc ggttgagaa agaaccgcc ggttccaaa 
        ggaagttcc taaacagaa ggttgagaa agtgattga 
        agagattag ggaagaacc gcccaagac tcccgtcaa 
        agagggagg cgttgcctt cgacgagct gggagatgt 
        ttggacaga agcgaaggt ggcgaagaa gagtaagaa 
        aaaagcgcg tagagataa ctagaagta gttcaacaa 
        atcagatat tgtatgtgt caggtatgt gattacgac 
        tatgtgcta cgagacaag atctcaaac cttaaaaag 
        acttaagta gctgattat cacagaatt ttgaactca 
        tcattcatt caaacatac tcaaaagaa taacgagtg 
        caacgtggc atgatagta ttcacaatc aaacgaaag 
        cttttttaa gtaaatgtc tagataaga ggtggcagc 
        atagctgca atattcaat gtgcattcc atgaggtca 
        tgtagtttt gtttgtttc attttgttg attttttca 
        tttagcaat ttgtatttt gtgccaata aaatcaaat 
        gaatgtctg acctatcca atgaaataa atggcctta 
        tagtcttgc agtgtgttg caaatatgc gatattttt 
        aataatata tttttgcga gaagagtaa caatcgtag 
        gttaacgtc ttccatatt tgttgtttg tttcatatt 
        gtcacactt cattgtaga tattttgct cagattttg 
        tgcatcagc ctaattaat aagttctaa ctaagcttc 
        aatctattg cttcctcga ccaatgaag gtaacatcc 
        ttcattgct tagagaagc tatgaaatt atttacttg 
        agattatgt gcatgtcca agcagcgac aaattgaaa 
        atcaaaatt tagcaatct tttgacatt tctgtggtg 
        gcatatagt ctcaagtga agggtcttg cctgatcaa 
        tatttttac tgcaagtag tcatttttt attgtagat 
        ttaaggtaa ataaatgta gtgtatttt tatttccta 
        taaactatt tttaatgcg tgtatcata ttgtaacta 
        atgtgtaaa attaaatat ttgatatgt tttcaatgt 
        aaagttttg ttgaatatt ctatgtact tttcatgtc 
        gactgtaac gggtacaca aaaacatat tgccctaac 
        gagcaagta tttatgata agaaaatta gtcattttt 
        attttagtt gcttgatac ggctcgagt ttgattatt 
        gtaaataac tcccgtatt tgatatttg aaatgtaca 
        ttgatcggc tttgtgtta gtttgtttt gctgtagag 
        acaccctaa ggtggttaa attgtttgt catctattt 
        attttagtc cagttttct ttgaatatg acaagctta 
        tttgtttcg tggaatcaa ataagccgc cgttgtgta 
        attaaattg aatacaaac ctgtattgt gtacatact 
        gtgttatta cattacttg tacatcgtt ttgtataag 
        gtctgtgga gtgttatct tttggggac cattttcag 
        aatatattt tgaaaaatt taaacttga attttcatt 
        atttaagtt taaattgaa acagttacc tcaagatcg 
        gaataataa cacctagta aaaatatct actttattt 
        taatttaca tgaaattat aaacatttg ttatgaaca 
        attattaca atagtaaag taatatata cgaatctgt 
        catttgcct ttagatatc tatcttcaa tattccacg 
        cacaatagc attttgttc actctcttt aacaaatgt 
        acggaggac taacactag gaggcaaat cactgtttt 
        ttcaaccta aaaaatata gcgcttact tgcggagaa 
        ctaaaaact aggaggcga a