General information | |||
TC010373 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 24641958..24648221 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC010373 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01677 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
Comment: larvae with reduced fluorescence were still moving, so the decreasing fluorescence isprobably not to the death of the larvae |
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
- >TC010373 RA
MELVKDLVN NQLTLYLLP VALVIIVVI LVFTFGFKS AEQPPFDKL TNDDRKQAG KKKKIKEKK TTANGHVST GDAKTEKSP VKDSKRSPT KDSTDSQKP KDKKIENNK QAEKNKKNE ANKNKVIEE VKNKKNLKK FLSEKPVDF DDGNWETVP SKADKKKKP DQSPAKKDK KSKKVEKAA EIEKLVKEV VNKELREKE KEIKVEKPV EEPIQIEEP VVVIEEKKE KKKEKKAKK ENEKPVEKE PPVPKEVPK QKVEKVIEE IREEPPKTP VKEGGVAFD ELGDVWTEA KVAKKSKKK ARRDN
- >TC010373 RA
atggaatta gtgaaagat cttgtaaat aaccaactc accctctac ctgttacct gtggctttg gtcattatt gtagtgatt ctagtgttc acattcgga ttcaaatcg gcagaacaa ccacctttc gacaaactc accaacgat gatcgaaaa caagcaggg aaaaagaag aaaatcaag gaaaagaaa accaccgcc aacggacac gtgtctact ggtgatgca aaaaccgag aaatcacca gtcaaagat tcaaaaagg tcgcctact aaagattcg accgactct caaaaacca aaagacaag aaaatagaa aacaacaaa caggccgaa aagaataag aagaacgaa gccaacaaa aataaagtg atcgaagaa gtgaaaaac aagaagaac ttgaagaaa ttcctcagt gagaaacca gtggatttc gacgatggc aactgggag accgttccc agcaaagcc gataagaag aagaaaccc gatcagagt ccagccaag aaggacaag aagtcgaaa aaggtcgag aaagcggcc gaaattgaa aagttagtc aaggaagtg gttaacaag gagctgcgt gagaaggaa aaggagatc aaggttgag aaacctgta gaggaaccg attcagatt gaggagcct gttgtcgtg attgaagag aagaaagaa aagaagaag gaaaagaag gccaagaag gagaatgaa aaaccggtt gagaaagaa ccgccggtt ccaaaggaa gttcctaaa cagaaggtt gagaaagtg attgaagag attagggaa gaaccgccc aagactccc gtcaaagag ggaggcgtt gccttcgac gagctggga gatgtttgg acagaagcg aaggtggcg aagaagagt aagaaaaaa gcgcgtaga gataactag
- >TC010373 RA
agactaaaa aatcagtaa gtcaattcc aataattac aataatttg cttttttcg agtcttttg aaatcatta attttgcaa aaaactagt caaaaaaac caccgtagt agtgttggc agtgtagaa ttccccaat ttgtcccta attttgaca gcttgcgca aatacgtgg cgcagaatg cagaaaata aacatatgt gtctgttta cgtcacagc cgcgtcacc aaaaattag atttgcggt ggttcgttg gttccgtgg cagcttttc ggtagaaat aatcacctt tcggtccat caaaatgga attagtgaa agatcttgt aaataacca actcaccct ctacctgtt acctgtggc tttggtcat tattgtagt gattctagt gttcacatt cggattcaa atcggcaga acaaccacc tttcgacaa actcaccaa cgatgatcg aaaacaagc agggaaaaa gaagaaaat caaggaaaa gaaaaccac cgccaacgg acacgtgtc tactggtga tgcaaaaac cgagaaatc accagtcaa agattcaaa aaggtcgcc tactaaaga ttcgaccga ctctcaaaa accaaaaga caagaaaat agaaaacaa caaacaggc cgaaaagaa taagaagaa cgaagccaa caaaaataa agtgatcga agaagtgaa aaacaagaa gaacttgaa gaaattcct cagtgagaa accagtgga tttcgacga tggcaactg ggagaccgt tcccagcaa agccgataa gaagaagaa acccgatca gagtccagc caagaagga caagaagtc gaaaaaggt cgagaaagc ggccgaaat tgaaaagtt agtcaagga agtggttaa caaggagct gcgtgagaa ggaaaagga gatcaaggt tgagaaacc tgtagagga accgattca gattgagga gcctgttgt cgtgattga agagaagaa agaaaagaa gaaggaaaa gaaggccaa gaaggagaa tgaaaaacc ggttgagaa agaaccgcc ggttccaaa ggaagttcc taaacagaa ggttgagaa agtgattga agagattag ggaagaacc gcccaagac tcccgtcaa agagggagg cgttgcctt cgacgagct gggagatgt ttggacaga agcgaaggt ggcgaagaa gagtaagaa aaaagcgcg tagagataa ctagaagta gttcaacaa atcagatat tgtatgtgt caggtatgt gattacgac tatgtgcta cgagacaag atctcaaac cttaaaaag acttaagta gctgattat cacagaatt ttgaactca tcattcatt caaacatac tcaaaagaa taacgagtg caacgtggc atgatagta ttcacaatc aaacgaaag cttttttaa gtaaatgtc tagataaga ggtggcagc atagctgca atattcaat gtgcattcc atgaggtca tgtagtttt gtttgtttc attttgttg attttttca tttagcaat ttgtatttt gtgccaata aaatcaaat gaatgtctg acctatcca atgaaataa atggcctta tagtcttgc agtgtgttg caaatatgc gatattttt aataatata tttttgcga gaagagtaa caatcgtag gttaacgtc ttccatatt tgttgtttg tttcatatt gtcacactt cattgtaga tattttgct cagattttg tgcatcagc ctaattaat aagttctaa ctaagcttc aatctattg cttcctcga ccaatgaag gtaacatcc ttcattgct tagagaagc tatgaaatt atttacttg agattatgt gcatgtcca agcagcgac aaattgaaa atcaaaatt tagcaatct tttgacatt tctgtggtg gcatatagt ctcaagtga agggtcttg cctgatcaa tatttttac tgcaagtag tcatttttt attgtagat ttaaggtaa ataaatgta gtgtatttt tatttccta taaactatt tttaatgcg tgtatcata ttgtaacta atgtgtaaa attaaatat ttgatatgt tttcaatgt aaagttttg ttgaatatt ctatgtact tttcatgtc gactgtaac gggtacaca aaaacatat tgccctaac gagcaagta tttatgata agaaaatta gtcattttt attttagtt gcttgatac ggctcgagt ttgattatt gtaaataac tcccgtatt tgatatttg aaatgtaca ttgatcggc tttgtgtta gtttgtttt gctgtagag acaccctaa ggtggttaa attgtttgt catctattt attttagtc cagttttct ttgaatatg acaagctta tttgtttcg tggaatcaa ataagccgc cgttgtgta attaaattg aatacaaac ctgtattgt gtacatact gtgttatta cattacttg tacatcgtt ttgtataag gtctgtgga gtgttatct tttggggac cattttcag aatatattt tgaaaaatt taaacttga attttcatt atttaagtt taaattgaa acagttacc tcaagatcg gaataataa cacctagta aaaatatct actttattt taatttaca tgaaattat aaacatttg ttatgaaca attattaca atagtaaag taatatata cgaatctgt catttgcct ttagatatc tatcttcaa tattccacg cacaatagc attttgttc actctcttt aacaaatgt acggaggac taacactag gaggcaaat cactgtttt ttcaaccta aaaaatata gcgcttact tgcggagaa ctaaaaact aggaggcga a
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
