Status
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Gene information
General information
TC010373 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 24641958..24648221
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC010373
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes

      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
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      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
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      Name
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      Email
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_01677

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      Comment: larvae with reduced fluorescence were still moving, so the decreasing fluorescence isprobably not to the death of the larvae
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
      Days post injection

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC010373 RA
        MELVKDLVN NQLTLYLLP VALVIIVVI LVFTFGFKS 
AEQPPFDKL TNDDRKQAG KKKKIKEKK TTANGHVST 
GDAKTEKSP VKDSKRSPT KDSTDSQKP KDKKIENNK 
QAEKNKKNE ANKNKVIEE VKNKKNLKK FLSEKPVDF 
DDGNWETVP SKADKKKKP DQSPAKKDK KSKKVEKAA 
EIEKLVKEV VNKELREKE KEIKVEKPV EEPIQIEEP 
VVVIEEKKE KKKEKKAKK ENEKPVEKE PPVPKEVPK 
QKVEKVIEE IREEPPKTP VKEGGVAFD ELGDVWTEA 
KVAKKSKKK ARRDN
      CDS sequence
      • >TC010373 RA
        atggaatta gtgaaagat cttgtaaat aaccaactc 
accctctac ctgttacct gtggctttg gtcattatt 
gtagtgatt ctagtgttc acattcgga ttcaaatcg 
gcagaacaa ccacctttc gacaaactc accaacgat 
gatcgaaaa caagcaggg aaaaagaag aaaatcaag 
gaaaagaaa accaccgcc aacggacac gtgtctact 
ggtgatgca aaaaccgag aaatcacca gtcaaagat 
tcaaaaagg tcgcctact aaagattcg accgactct 
caaaaacca aaagacaag aaaatagaa aacaacaaa 
caggccgaa aagaataag aagaacgaa gccaacaaa 
aataaagtg atcgaagaa gtgaaaaac aagaagaac 
ttgaagaaa ttcctcagt gagaaacca gtggatttc 
gacgatggc aactgggag accgttccc agcaaagcc 
gataagaag aagaaaccc gatcagagt ccagccaag 
aaggacaag aagtcgaaa aaggtcgag aaagcggcc 
gaaattgaa aagttagtc aaggaagtg gttaacaag 
gagctgcgt gagaaggaa aaggagatc aaggttgag 
aaacctgta gaggaaccg attcagatt gaggagcct 
gttgtcgtg attgaagag aagaaagaa aagaagaag 
gaaaagaag gccaagaag gagaatgaa aaaccggtt 
gagaaagaa ccgccggtt ccaaaggaa gttcctaaa 
cagaaggtt gagaaagtg attgaagag attagggaa 
gaaccgccc aagactccc gtcaaagag ggaggcgtt 
gccttcgac gagctggga gatgtttgg acagaagcg 
aaggtggcg aagaagagt aagaaaaaa gcgcgtaga 
gataactag
      mRNA sequence
      • >TC010373 RA
        agactaaaa aatcagtaa gtcaattcc aataattac 
aataatttg cttttttcg agtcttttg aaatcatta 
attttgcaa aaaactagt caaaaaaac caccgtagt 
agtgttggc agtgtagaa ttccccaat ttgtcccta 
attttgaca gcttgcgca aatacgtgg cgcagaatg 
cagaaaata aacatatgt gtctgttta cgtcacagc 
cgcgtcacc aaaaattag atttgcggt ggttcgttg 
gttccgtgg cagcttttc ggtagaaat aatcacctt 
tcggtccat caaaatgga attagtgaa agatcttgt 
aaataacca actcaccct ctacctgtt acctgtggc 
tttggtcat tattgtagt gattctagt gttcacatt 
cggattcaa atcggcaga acaaccacc tttcgacaa 
actcaccaa cgatgatcg aaaacaagc agggaaaaa 
gaagaaaat caaggaaaa gaaaaccac cgccaacgg 
acacgtgtc tactggtga tgcaaaaac cgagaaatc 
accagtcaa agattcaaa aaggtcgcc tactaaaga 
ttcgaccga ctctcaaaa accaaaaga caagaaaat 
agaaaacaa caaacaggc cgaaaagaa taagaagaa 
cgaagccaa caaaaataa agtgatcga agaagtgaa 
aaacaagaa gaacttgaa gaaattcct cagtgagaa 
accagtgga tttcgacga tggcaactg ggagaccgt 
tcccagcaa agccgataa gaagaagaa acccgatca 
gagtccagc caagaagga caagaagtc gaaaaaggt 
cgagaaagc ggccgaaat tgaaaagtt agtcaagga 
agtggttaa caaggagct gcgtgagaa ggaaaagga 
gatcaaggt tgagaaacc tgtagagga accgattca 
gattgagga gcctgttgt cgtgattga agagaagaa 
agaaaagaa gaaggaaaa gaaggccaa gaaggagaa 
tgaaaaacc ggttgagaa agaaccgcc ggttccaaa 
ggaagttcc taaacagaa ggttgagaa agtgattga 
agagattag ggaagaacc gcccaagac tcccgtcaa 
agagggagg cgttgcctt cgacgagct gggagatgt 
ttggacaga agcgaaggt ggcgaagaa gagtaagaa 
aaaagcgcg tagagataa ctagaagta gttcaacaa 
atcagatat tgtatgtgt caggtatgt gattacgac 
tatgtgcta cgagacaag atctcaaac cttaaaaag 
acttaagta gctgattat cacagaatt ttgaactca 
tcattcatt caaacatac tcaaaagaa taacgagtg 
caacgtggc atgatagta ttcacaatc aaacgaaag 
cttttttaa gtaaatgtc tagataaga ggtggcagc 
atagctgca atattcaat gtgcattcc atgaggtca 
tgtagtttt gtttgtttc attttgttg attttttca 
tttagcaat ttgtatttt gtgccaata aaatcaaat 
gaatgtctg acctatcca atgaaataa atggcctta 
tagtcttgc agtgtgttg caaatatgc gatattttt 
aataatata tttttgcga gaagagtaa caatcgtag 
gttaacgtc ttccatatt tgttgtttg tttcatatt 
gtcacactt cattgtaga tattttgct cagattttg 
tgcatcagc ctaattaat aagttctaa ctaagcttc 
aatctattg cttcctcga ccaatgaag gtaacatcc 
ttcattgct tagagaagc tatgaaatt atttacttg 
agattatgt gcatgtcca agcagcgac aaattgaaa 
atcaaaatt tagcaatct tttgacatt tctgtggtg 
gcatatagt ctcaagtga agggtcttg cctgatcaa 
tatttttac tgcaagtag tcatttttt attgtagat 
ttaaggtaa ataaatgta gtgtatttt tatttccta 
taaactatt tttaatgcg tgtatcata ttgtaacta 
atgtgtaaa attaaatat ttgatatgt tttcaatgt 
aaagttttg ttgaatatt ctatgtact tttcatgtc 
gactgtaac gggtacaca aaaacatat tgccctaac 
gagcaagta tttatgata agaaaatta gtcattttt 
attttagtt gcttgatac ggctcgagt ttgattatt 
gtaaataac tcccgtatt tgatatttg aaatgtaca 
ttgatcggc tttgtgtta gtttgtttt gctgtagag 
acaccctaa ggtggttaa attgtttgt catctattt 
attttagtc cagttttct ttgaatatg acaagctta 
tttgtttcg tggaatcaa ataagccgc cgttgtgta 
attaaattg aatacaaac ctgtattgt gtacatact 
gtgttatta cattacttg tacatcgtt ttgtataag 
gtctgtgga gtgttatct tttggggac cattttcag 
aatatattt tgaaaaatt taaacttga attttcatt 
atttaagtt taaattgaa acagttacc tcaagatcg 
gaataataa cacctagta aaaatatct actttattt 
taatttaca tgaaattat aaacatttg ttatgaaca 
attattaca atagtaaag taatatata cgaatctgt 
catttgcct ttagatatc tatcttcaa tattccacg 
cacaatagc attttgttc actctcttt aacaaatgt 
acggaggac taacactag gaggcaaat cactgtttt 
ttcaaccta aaaaatata gcgcttact tgcggagaa 
ctaaaaact aggaggcga a
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