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Gene information
General information
TC010353 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid24: 95533..102464
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010353
    iBeetle screen iB_01672
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    remarks: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010353 RA
      MGCSCCTIK VLLVCVVIS VALLIVSLA LAFKVFPDL 
      LESEVNKAV RLEDGTKQY DRFVELPFP VDFKVYLFN 
      VSNPQQVLD GTEKPKLEE IGPFVYKQY RKKTILGKN 
      EEEDTISYT QKETFEFDA EASKPLTEE SVVTVLNPA 
      LMSIYQLAE DLHLAGAAD TCIKQTFEN NQGKVFIEA 
      NVRKLLFDG FSFCKNTSP GICGLVNDL ICAIAATKR 
      NSDLVLPDY SLIFSYLNY KRKPDDGKY TVKRGLTNI 
      EKLGHIVAW NDSLYTKFW GEGTTCSEV KGTDSTLYP 
      PRVTTDSAF YIYSTDICR FVKINYKGE ESYKGIDGY 
      LFETSEDTL RSSAPEEDC YCSKLSRDM EGKKSCFLD 
      GVIDMQTCF GVPVLFSFP HFLWADNKY LSAVEGLNP 
      VEEKHKTYL VVEPNTGTP LKGMKRIQL NGVIRPIVG 
      IKSMLQTKR ALLPLLWIE EGVSLPQKY VDELKSSYF 
      DKVQIVDGV RYALIVISA ILVGAFGII ILRKRSHAK 
      HHV
    CDS sequence
    • >TC010353 RA
      atggggtgt tcgtgttgt accatcaaa gtattacta 
      gtttgtgta gtaatcagt gtagccctt ttgatagtg 
      tccctagcg ctagctttt aaggtattt cccgacctc 
      ctggaatct gaagtaaat aaggctgtt aggctcgag 
      gatggcact aaacagtat gacagattt gttgaacta 
      ccgtttcca gttgacttc aaagtgtac ctctttaac 
      gtctcaaac ccccaacaa gttctagat ggaactgaa 
      aaacctaaa ctcgaggaa attggaccc tttgtgtac 
      aagcaatac aggaaaaag acaatactg ggcaaaaat 
      gaagaagaa gacacaatt tcatacaca caaaaggaa 
      actttcgaa tttgacgcc gaggcatct aaaccgcta 
      acagaagag tcagttgta acggttttg aatcccgca 
      ttgatgtca atttatcaa ttggcggag gatcttcac 
      ttggccggg gccgcagac acttgcatc aagcaaaca 
      tttgagaac aaccaaggc aaagttttt atagaagca 
      aacgtgcga aaactgctg tttgacggc tttagtttc 
      tgcaagaac acttcgcct ggaatatgt ggccttgta 
      aacgaccta atttgtgcg attgcagcg acgaaaaga 
      aattcggac cttgtcttg cccgactat tctttgata 
      ttttcctat ttgaactac aaacgaaag ccggatgat 
      ggcaaatat accgtgaag agaggtctc accaacatt 
      gaaaaactg ggacatatt gtggcatgg aatgacagt 
      ttgtatacg aaattttgg ggggaaggc accacctgc 
      agtgaagtt aaagggacc gattcgacc ctttatccc 
      cctagggtt acaacagat agtgcattt tacatctat 
      tcgacggac atctgcagg tttgtgaaa atcaattac 
      aaaggagaa gagagttac aaaggcatc gatggctat 
      ttatttgaa accagtgag gacactttg cgaagctcc 
      gcccctgag gaagactgc tattgcagt aaattgagc 
      agagacatg gaaggcaaa aagagttgt ttcttggat 
      ggagtaata gacatgcaa acttgtttc ggagtgcca 
      gttcttttc tcattccca cacttcttg tgggccgat 
      aacaagtac ttaagtgca gtggaagga ctaaaccct 
      gtcgaggaa aaacacaaa acgtatctc gttgtcgag 
      ccaaatact ggaactcct ctgaaagga atgaaaagg 
      attcaactg aatggagtc attaggccg attgttgga 
      ataaaatca atgttgcaa accaagcga gctttattg 
      ccgcttttg tggatagaa gaaggagtg tctctgccc 
      caaaaatac gtcgatgaa ctaaaatcg agctacttt 
      gacaaggtt cagatagtc gatggtgtt agatacgct 
      cttatagta atatcagcc atattggtt ggtgctttt 
      ggaattatt attttacga aaacgttca cacgctaaa 
      caccatgta taa
    mRNA sequence
    • >TC010353 RA
      aatagattt atctcatca cttccaata acttataaa 
      cactttaat gtggacact gaaatagtt taggcgcaa 
      aatggggtg ttcgtgttg taccatcaa agtattact 
      agtttgtgt agtaatcag tgtagccct tttgatagt 
      gtccctagc gctagcttt taaggtatt tcccgacct 
      cctggaatc tgaagtaaa taaggctgt taggctcga 
      ggatggcac taaacagta tgacagatt tgttgaact 
      accgtttcc agttgactt caaagtgta cctctttaa 
      cgtctcaaa cccccaaca agttctaga tggaactga 
      aaaacctaa actcgagga aattggacc ctttgtgta 
      caagcaata caggaaaaa gacaatact gggcaaaaa 
      tgaagaaga agacacaat ttcatacac acaaaagga 
      aactttcga atttgacgc cgaggcatc taaaccgct 
      aacagaaga gtcagttgt aacggtttt gaatcccgc 
      attgatgtc aatttatca attggcgga ggatcttca 
      cttggccgg ggccgcaga cacttgcat caagcaaac 
      atttgagaa caaccaagg caaagtttt tatagaagc 
      aaacgtgcg aaaactgct gtttgacgg ctttagttt 
      ctgcaagaa cacttcgcc tggaatatg tggccttgt 
      aaacgacct aatttgtgc gattgcagc gacgaaaag 
      aaattcgga ccttgtctt gcccgacta ttctttgat 
      attttccta tttgaacta caaacgaaa gccggatga 
      tggcaaata taccgtgaa gagaggtct caccaacat 
      tgaaaaact gggacatat tgtggcatg gaatgacag 
      tttgtatac gaaattttg gggggaagg caccacctg 
      cagtgaagt taaagggac cgattcgac cctttatcc 
      ccctagggt tacaacaga tagtgcatt ttacatcta 
      ttcgacgga catctgcag gtttgtgaa aatcaatta 
      caaaggaga agagagtta caaaggcat cgatggcta 
      tttatttga aaccagtga ggacacttt gcgaagctc 
      cgcccctga ggaagactg ctattgcag taaattgag 
      cagagacat ggaaggcaa aaagagttg tttcttgga 
      tggagtaat agacatgca aacttgttt cggagtgcc 
      agttctttt ctcattccc acacttctt gtgggccga 
      taacaagta cttaagtgc agtggaagg actaaaccc 
      tgtcgagga aaaacacaa aacgtatct cgttgtcga 
      gccaaatac tggaactcc tctgaaagg aatgaaaag 
      gattcaact gaatggagt cattaggcc gattgttgg 
      aataaaatc aatgttgca aaccaagcg agctttatt 
      gccgctttt gtggataga agaaggagt gtctctgcc 
      ccaaaaata cgtcgatga actaaaatc gagctactt 
      tgacaaggt tcagatagt cgatggtgt tagatacgc 
      tcttatagt aatatcagc catattggt tggtgcttt 
      tggaattat tattttacg aaaacgttc acacgctaa 
      acaccatgt ataataata ggtggacac aaattaata 
      ttatatatt tgtgttgta aatacaatc ttacccagt 
      at