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Gene information
General information
TC010336 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid24: 839657..852604
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010336
    iBeetle screen iB_01669
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    egg chamber colour lighter - Number of animals showing the phenotype: 4
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection



    Pupal morphology
    wings shortened - Number of animals showing the phenotype: 6

    dorsal thoracic muscles fluorescence decreased - Number of animals showing the phenotype: 6

    oblique dorsal thoracic muscles shortened - Number of animals showing the phenotype: 6
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010336 RA
      MDSEDSRDA SSDNESERK GPHSPASSR SRSRASQAA 
      SVLSRSASP QSGPESPRS ALSRSTSPE DRSRSGPKS 
      PASASSRSS PENPPNGDG PRSPSPANS SRSSSPKSP 
      KRSESRSSK HSLSPDRVT NVSDRSGSS AAKRSDNED 
      GNTSDSSVA SVDSSSRKN KNITKNVDT LPEEISDGD 
      MESDHEKEP ERKKEEPKN VRQEININH EDLSDVSDL 
      DESIGGHSD DERDKNEEK KQNGETKKE DVEIPISEK 
      TSRPEDGEE QLDFEAEDG ECEMEEKKD KDEKDTGSL 
      VEDGEMAEQ KEKEKEELE EGEVTDEDE VRPEETEPR 
      PVCRFFSRG QCTWGASCR FLHPGVTDK GNYTMFELI 
      RPVVPGEYG REERSYGRP EPAPVESAW ERGLRTAKE 
      MVRKSIKRK EQDIDFEEK KMNLSLAQE EFDKENGYY 
      GRVASPEPR YVRHVPVEY PPVVRPPPA EEYYGRRQV 
      IYEPDYVAP PRERVRTYR ELPSHRMPD FYNNKYEDE 
      AVPASTKTR KPKREVIVQ RAAEERPVR GDEWSDPWM 
      RSKSPAGRK ASGRKRSYS SGSSYSSSS SSRSSSESS 
      RSSFRSRSR SHKRSSRSH RSRHVSPSV IVSERKAAN 
      ERAALMNPP APRKRAPSP GMMRVSATN PPAPVKEDQ 
      FGRTRSKMA IAAALARVK GRRRSSKSS SDSSGSSSS 
      DSDSSGSSS SSSREESSP RRTSGVDIS IAKAMDALK 
      GSSSEKRQI KLNLKNPVG ARKPEISDI ARKTEALQG 
      KKRPASSPP PLDPKSKKA TSRREELLK QLKAVEDAI 
      AKKRSKVN
    CDS sequence
    • >TC010336 RA
      atggactcg gaagattcg cgtgacgca tcttccgac 
      aacgaaagc gagagaaaa gggccccac agcccggcc 
      agttctagg tctaggagc cgagcctcg caagcggcc 
      tcggtccta agtcggtcg gcgtccccg caaagcggg 
      cccgagtcg ccccggtcg gccttaagc aggtcgacg 
      tccccggaa gacaggtcg cgaagcggc cccaagtca 
      ccggccagt gcctcgtcg cgtagctcc cccgagaac 
      cccccgaac ggcgacggc cccagatcc ccctctcct 
      gccaactcc tccaggtcc agctccccc aaaagcccc 
      aaacgctca gaaagtcgt agctcaaaa cattcactc 
      tcgcctgat cgggtcact aacgtttcc gacagatcg 
      ggctcctcg gccgccaag aggtccgac aatgaggac 
      ggaaacacg tccgattcg agcgtcgcc agtgtggat 
      tcctccagt cgcaagaat aaaaatatc acgaaaaat 
      gttgataca ctaccagag gaaatttcc gacggtgat 
      atggaatca gaccatgaa aaggagccg gaacgcaaa 
      aaggaagaa ccgaagaat gtgagacag gaaatcaat 
      attaaccat gaagacctg tccgacgtt tccgattta 
      gacgaatct attggaggc cattcggac gacgaacgc 
      gacaaaaac gaggagaaa aaacagaac ggagaaacg 
      aaaaaagaa gacgtcgaa atacccatt tcggaaaaa 
      acaagtcgg ccggaagac ggtgaagaa cagttggat 
      tttgaggca gaagatggt gaatgcgaa atggaggaa 
      aaaaaggac aaagatgag aaagataca ggttctttg 
      gttgaggat ggcgaaatg gcggaacaa aaagagaag 
      gaaaaggag gagcttgaa gagggtgaa gttacagat 
      gaggacgag gtcaggcct gaagagaca gaacctaga 
      cccgtgtgc agatttttc tcgaggggg cagtgcaca 
      tggggggcg agttgtcga tttttacat ccaggggtt 
      acggataaa ggaaattat acaatgttt gaattgatt 
      cggcctgtc gttcctggt gaatacgga agagaggag 
      cgatcctac ggtagacca gaaccagca cctgttgaa 
      tccgcttgg gaacgaggt cttcgcaca gccaaagaa 
      atggtccga aaatcgata aaacgtaaa gaacaagac 
      atcgatttt gaagaaaag aaaatgaat ttgagtctg 
      gcacaagag gaatttgac aaagaaaac ggttattac 
      ggacgcgtt gcatcgcca gaacccaga tatgtaaga 
      cacgttccc gttgaatat ccaccagtc gttcggcca 
      ccaccagct gaagaatat tacggccga agacaagtc 
      atatacgaa ccagattac gtagcaccg ccacgagaa 
      cgtgtccga acttacaga gaacttcca tcacataga 
      atgcccgat ttttacaat aataagtac gaagatgag 
      gcagttcca gcaagcaca aaaacccgc aagcctaaa 
      cgtgaagtc atcgttcag agagcagca gaagaacga 
      cctgtgcga ggagacgaa tggtcggat ccttggatg 
      cggtcaaaa tcaccagca ggtcgcaaa gcttcggga 
      cgaaaacgt tcgtacagt tccggttcg agttattct 
      tccagcagt tcgtctaga agttcatcg gaatctagt 
      cgtagttca ttccggtca agatcgagg tctcacaag 
      agatcaagt cgttcgcat cgcagtaga cacgtctcg 
      ccatctgtt attgtttca gagagaaaa gccgccaat 
      gagcgagcg gctttaatg aacccgcca gctccgcga 
      aaacgagca ccctcacct ggaatgatg agggttagt 
      gcaacaaat cctcccgca ccggtcaag gaggatcag 
      ttcggtcgc acgagaagt aaaatggca atagctgcg 
      gctttagca agggtgaaa gggcggcga agatcttcg 
      aagtcgtct tcggatagt tccggatcg agttcaagt 
      gattcggat tcctcagga tcgagtagt tcgtcaagt 
      cgggaggaa agttcgccg cgaaggacg agtggtgtg 
      gatatttca atagccaaa gccatggat gcgttgaaa 
      ggaagttcg tcggagaaa cggcaaatc aaactaaat 
      ttgaaaaac ccagtaggt gcgcgtaag ccggaaatt 
      tcggacatt gcgcgtaaa acggaagcg ttacaaggt 
      aagaaacgt ccggcgagt tcgccgcct ccattggat 
      cccaagtcg aagaaggcg acgtcacga agagaggag 
      ttactcaaa cagttgaaa gcggtggag gatgccata 
      gcaaaaaag aggtcaaaa gtgaattga
    mRNA sequence
    • >TC010336 RA
      cgggacgcc atttcctga agcaaaaag tggcaggga 
      gaggtgtct cgagtcaat gttgttaaa tcgaaatta 
      aaagcaatt ttgtgtgag aaaggccat tgcgttctg 
      ctgcaggaa ctgccgaac aaaaaccaa gcctaaatc 
      actgttaaa atggactcg gaagattcg cgtgacgca 
      tcttccgac aacgaaagc gagagaaaa gggccccac 
      agcccggcc agttctagg tctaggagc cgagcctcg 
      caagcggcc tcggtccta agtcggtcg gcgtccccg 
      caaagcggg cccgagtcg ccccggtcg gccttaagc 
      aggtcgacg tccccggaa gacaggtcg cgaagcggc 
      cccaagtca ccggccagt gcctcgtcg cgtagctcc 
      cccgagaac cccccgaac ggcgacggc cccagatcc 
      ccctctcct gccaactcc tccaggtcc agctccccc 
      aaaagcccc aaacgctca gaaagtcgt agctcaaaa 
      cattcactc tcgcctgat cgggtcact aacgtttcc 
      gacagatcg ggctcctcg gccgccaag aggtccgac 
      aatgaggac ggaaacacg tccgattcg agcgtcgcc 
      agtgtggat tcctccagt cgcaagaat aaaaatatc 
      acgaaaaat gttgataca ctaccagag gaaatttcc 
      gacggtgat atggaatca gaccatgaa aaggagccg 
      gaacgcaaa aaggaagaa ccgaagaat gtgagacag 
      gaaatcaat attaaccat gaagacctg tccgacgtt 
      tccgattta gacgaatct attggaggc cattcggac 
      gacgaacgc gacaaaaac gaggagaaa aaacagaac 
      ggagaaacg aaaaaagaa gacgtcgaa atacccatt 
      tcggaaaaa acaagtcgg ccggaagac ggtgaagaa 
      cagttggat tttgaggca gaagatggt gaatgcgaa 
      atggaggaa aaaaaggac aaagatgag aaagataca 
      ggttctttg gttgaggat ggcgaaatg gcggaacaa 
      aaagagaag gaaaaggag gagcttgaa gagggtgaa 
      gttacagat gaggacgag gtcaggcct gaagagaca 
      gaacctaga cccgtgtgc agatttttc tcgaggggg 
      cagtgcaca tggggggcg agttgtcga tttttacat 
      ccaggggtt acggataaa ggaaattat acaatgttt 
      gaattgatt cggcctgtc gttcctggt gaatacgga 
      agagaggag cgatcctac ggtagacca gaaccagca 
      cctgttgaa tccgcttgg gaacgaggt cttcgcaca 
      gccaaagaa atggtccga aaatcgata aaacgtaaa 
      gaacaagac atcgatttt gaagaaaag aaaatgaat 
      ttgagtctg gcacaagag gaatttgac aaagaaaac 
      ggttattac ggacgcgtt gcatcgcca gaacccaga 
      tatgtaaga cacgttccc gttgaatat ccaccagtc 
      gttcggcca ccaccagct gaagaatat tacggccga 
      agacaagtc atatacgaa ccagattac gtagcaccg 
      ccacgagaa cgtgtccga acttacaga gaacttcca 
      tcacataga atgcccgat ttttacaat aataagtac 
      gaagatgag gcagttcca gcaagcaca aaaacccgc 
      aagcctaaa cgtgaagtc atcgttcag agagcagca 
      gaagaacga cctgtgcga ggagacgaa tggtcggat 
      ccttggatg cggtcaaaa tcaccagca ggtcgcaaa 
      gcttcggga cgaaaacgt tcgtacagt tccggttcg 
      agttattct tccagcagt tcgtctaga agttcatcg 
      gaatctagt cgtagttca ttccggtca agatcgagg 
      tctcacaag agatcaagt cgttcgcat cgcagtaga 
      cacgtctcg ccatctgtt attgtttca gagagaaaa 
      gccgccaat gagcgagcg gctttaatg aacccgcca 
      gctccgcga aaacgagca ccctcacct ggaatgatg 
      agggttagt gcaacaaat cctcccgca ccggtcaag 
      gaggatcag ttcggtcgc acgagaagt aaaatggca 
      atagctgcg gctttagca agggtgaaa gggcggcga 
      agatcttcg aagtcgtct tcggatagt tccggatcg 
      agttcaagt gattcggat tcctcagga tcgagtagt 
      tcgtcaagt cgggaggaa agttcgccg cgaaggacg 
      agtggtgtg gatatttca atagccaaa gccatggat 
      gcgttgaaa ggaagttcg tcggagaaa cggcaaatc 
      aaactaaat ttgaaaaac ccagtaggt gcgcgtaag 
      ccggaaatt tcggacatt gcgcgtaaa acggaagcg 
      ttacaaggt aagaaacgt ccggcgagt tcgccgcct 
      ccattggat cccaagtcg aagaaggcg acgtcacga 
      agagaggag ttactcaaa cagttgaaa gcggtggag 
      gatgccata gcaaaaaag aggtcaaaa gtgaattga 
      aatcgattt atcacagca attccttta taagggatg 
      tttgcctaa ccagtgttt cttacacac gatttttgt 
      gtggatttt gaatcggca ctgtttgta gaattgtgt 
      cattgatgt ttttcatga atgatttca aattaggtg 
      ctttttttc tagagtagg ttattaaat gactgacgt 
      ttagcgtta tgattacta tatcgtagt aatttaatt 
      cacagtaaa ttgaattgt gattttaat agcatgtaa 
      catattaat gtaataaaa acaaccttc attttattc 
      gtttgttta ggtggttct atgtggatt tttcatgtt 
      agtattaac gggtgccta cagtgaatt taaagactt 
      ctattctcg aatgaaagt attatttta cacttgttt 
      actggtcaa tttgctagc tggttgaaa agatgtaca 
      cttcatgac aattttact cttttttaa agttttgtg 
      taaattttg gcaaacaaa cgtctgtat aaagtaagt 
      agaacgtgg actttaata ttctggatt atttctaaa 
      tattttgat atttataat ttaagtaaa gtacatatt 
      tgcagattt gatttttat ttattttga taacctacc 
      cgtgttatt gacgacata