Status
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Gene information
General information
TC010322 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 25309289..25320945
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010322
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01666

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010322 RA
      MYLYNLTLQ RATAITHAI HGNFSGTKM QEIVISRGK 
      SLEVLRPDP NTGKVHTLL TVEIFGVVR SLMSFRLTG 
      GTKDFVIVG SDSGRIVIL EYIPSKNNF EKVHQETFG 
      KSGVRRIVP GQYLATDPR GRAVMIGAV EKQKLAYIL 
      NRDVQARLT ISSPLEAHK SNTLVYHMV GVDVGYDNP 
      MFACLEIDY EEADSDPSG EAAQKTQQT LTFYELDLG 
      VNHVVRKYS EPLEEHANF LVTVPGGDD GPSGVLICS 
      ENYLTYKNL GDQHDIRCP IPRRRNDLD DPERGMIFV 
      CSATHKTKS MFFFLAQTE QGDIFKITL ETDEDMVTE 
      IKLKYFDTV PVASAMCVL KTGFLFVTS EFGNHYLYQ 
      IAHLGDDDD ELEFSSAMP LEEGDTFFF APRSLRNLV 
      LVDEMESLS PILSCRVAD LAGEDTPQL YMLCGRGPR 
      SSLRVLRHG LEVSEMAVS ELPGNPNAV WTVKRRSDD 
      EYDAYIIVS FVNATLVLS IGETVEEVT DSGFLGTTP 
      TLSCSALSD DALVQVYPG GIRHICSDK RVNEWKAPG 
      KKTIVKCAI NQRQVVIAL SGGELAYFE MDPTGQLHE 
      YKERKRMNA DVVCMALAN VAPGEQLSL FLAVGLADS 
      TVRIISLDP SDCLAPRSI QGLPVCAES LCIVEMGCT 
      DREPDNAAA ASTTSTLYL NIGLTNGAL LRNVLDPVS 
      GELSDTRTR YLGSRPVKL FRIRMQQSE AVLAMSSRS 
      WLSYYYQSR FYLTPLSYE SLEYASGFS SEQCPEGIV 
      AISTNTLRI LALEKLGAV FNQVSFPLE YTPRKFIIH 
      PESNNLLII ETEHNAYTE ETKKQRRLQ MAEEMKEAA 
      GEEEQELAK EMADAFLNE DLPESIFSA PKAGHGMWA 
      STLKIMDPV QGIVHKCIR LEQNEAAMS SVLVKFQNQ 
      PQQTLFLIV GISKDFQLN PRHCNTGFL DTYKMDPMG 
      RELEFVHRT PVDEVPMAL CAYNGLLLA GVGRMLRLY 
      DMGKKKLLR KCENKHIPN AIVNIQAMG KRIFVSDVQ 
      ESVFMVRYK RAENQLIIF ADDTHPRWV TCNCVLDYD 
      TVAVADKFG NIAILRLPP NVSDDVEED PTGHKSLWD 
      RGLLNGASQ KADTIATFH VGETVTWLQ KATLIPGGW 
      ESLIYTTLS GSVGVLVPF TSREDHDFF QHLEMHMRS 
      ENSPLCGRD HLSFRSYYF PVKNVIDGD LCEQYNSLE 
      PVKQKSIAS DLDRTPAEV SKKLEDIRT RYAF
    CDS sequence
    • >TC010322 RA
      atgtatctc tacaacctg accttacaa agggccaca 
      gccatcacc cacgccatc catggcaat ttttccggc 
      accaaaatg caagaaatt gtcatttca cgtggcaaa 
      agcttggaa gtgctacgg cctgacccc aacaccggt 
      aaagtccac acgcttcta accgtcgaa atattcgga 
      gtcgtccgc tcgttgatg tcgtttcga ctcaccgga 
      ggcaccaaa gacttcgtc atcgtgggc tccgactcc 
      ggcagaatc gttattctt gagtatatc ccgtctaaa 
      aacaacttc gagaaggtc caccaggag acgttcggc 
      aaatcgggg gtcagacgt attgttcca ggccagtac 
      ctagccact gaccctcga ggacgtgct gtaatgata 
      ggcgccgtc gagaaacaa aaactcgct tacattttg 
      aatagagac gtgcaagcc cgcttgacg atctcctcg 
      cccctcgaa gcccacaaa agcaacact cttgtctat 
      cacatggtg ggggtggac gtcggttat gacaaccct 
      atgttcgct tgcctcgaa atcgactac gaagaagca 
      gattcggat ccttctggc gaagcggct caaaaaact 
      caacaaacc ttgaccttc tacgagctg gacctgggt 
      gtaaatcac gttgtgcgg aaatactcg gaaccgttg 
      gaggaacac gcgaatttc ttagtcacg gttccaggg 
      ggcgacgat ggcccttca ggagtcctt atttgttca 
      gagaattat ttgacttat aagaatttg ggcgatcag 
      cacgatatt cgttgcccg attcccaga aggcggaac 
      gatttggat gacccagag agaggcatg atttttgtc 
      tgttctgcc acgcataag acgaagtcg atgttcttt 
      ttcctggca cagacggag caaggggat atctttaaa 
      atcactctc gaaacggat gaagatatg gtcacggaa 
      atcaagtta aagtatttc gatactgtt ccggtcgct 
      tccgctatg tgtgtgttg aagacgggg ttcttgttt 
      gtaacttcg gaatttggc aaccactac ttgtatcag 
      atcgcccat cttggtgac gacgacgat gagttggag 
      tttagctcc gctatgccc ttagaagaa ggtgacact 
      ttttttttc gcgcctcgt tcccttcgt aacttggtt 
      ttggtcgac gaaatggaa tcgctttca ccgattcta 
      tcttgtcga gttgctgat ctggcaggt gaagacact 
      cctcaattg tatatgctt tgcggaaga ggcccgaga 
      tcctcattg cgggttcta cgacacggt ctcgaagtg 
      tctgagatg gccgtgtcc gagcttcct ggcaatccc 
      aacgccgtt tggacggtt aagagaaga agtgacgat 
      gaatatgat gcttatatt attgtgtct ttcgttaac 
      gcaactttg gtattgtca attggtgaa actgtggag 
      gaggttaca gacagtggc tttttgggt acgacacca 
      acattgtcc tgttcagct cttagtgac gatgcctta 
      gttcaggtg tatccaggc ggtattcgg catatttgt 
      tccgataag cgtgtaaat gagtggaaa gctccaggg 
      aagaaaaca atcgtaaag tgtgcaatt aatcaacga 
      caagtggtt attgcactg tcagggggt gaactagct 
      tacttcgaa atggatccg acgggccaa ttacacgaa 
      tacaaagaa cgaaaacgt atgaacgcc gacgtcgtt 
      tgcatggca ttagcaaac gtggcgccc ggcgaacaa 
      ttatccctc ttccttgcc gtgggatta gccgatagt 
      acagtacgg attatctcg ctagatccg agcgattgt 
      ttggcgccg cgttctatc caaggatta cccgtttgt 
      gccgagtct ttatgtatc gtagaaatg ggttgcaca 
      gatcgtgaa ccggataac gctgcagct gccagcaca 
      acaagcacc ttatatctg aacattgga cttacaaac 
      ggcgcttta cttcgcaac gtattagat ccagtttct 
      ggagaattg tcagacaca agaacgaga tatttgggt 
      tcgagaccg gtcaaactc ttccgaata cgaatgcaa 
      caatcggaa gctgttctg gcaatgagt agccgttct 
      tggttgagt tattactac caaagtcgg ttttatttg 
      actccactc tcgtatgaa agtctggaa tatgcctca 
      gggttcagt tccgagcag tgtccagaa ggcattgtt 
      gcgatctcc acaaatacg ttacgtatt ttggcgttg 
      gaaaaacta ggggctgtt tttaatcaa gtctcattt 
      ccgcttgaa tacaccccg aggaagttt ataatccat 
      cctgaaagc aataatttg ctgatcatt gaaaccgag 
      cacaatgcc tacaccgaa gaaaccaaa aaacaacga 
      agattacaa atggccgaa gaaatgaaa gaagccgca 
      ggtgaagaa gaacaagag ttggctaaa gaaatggcc 
      gatgcgttt ttgaatgaa gacttgccc gaaagtatt 
      ttttcggcg cctaaagcc gggcatgga atgtgggct 
      tcgacgctt aaaataatg gatccggta caaggaatc 
      gtgcataag tgtatacga ttggaacag aacgaggca 
      gcaatgtct agtgttttg gttaaattc caaaatcag 
      ccacaacaa actctgttc ttgattgtt ggcatttct 
      aaagatttt caactaaat ccaaggcat tgtaacact 
      gggtttttg gatacttat aaaatggat ccaatgggt 
      cgggagttg gaatttgtt catagaact cctgttgat 
      gaagttcca atggccctt tgtgcttat aatggactg 
      ctacttgca ggagttggc aggatgttg aggctttac 
      gatatgggg aagaagaaa ctcttaagg aaatgtgaa 
      aacaagcac ataccaaac gctattgtg aatattcaa 
      gccatgggc aaacgaatt ttcgtttcg gacgttcaa 
      gagtctgtt ttcatggtt cggtacaag agagctgaa 
      aatcagttg attatattt gccgatgac acgcaccct 
      cgttgggtt acttgcaac tgtgtgctt gattatgat 
      acagtggct gttgccgac aagtttggt aatattgca 
      attttacgt ttgccacca aatgtaagt gatgatgtt 
      gaagaggat cctactgga cataaatcg ttatgggat 
      agaggactg ctaaatggg gcgtcacaa aaagctgat 
      actattgca acattccac gttggtgag actgtcacg 
      tggttgcaa aaagccaca ctgattcct ggaggttgg 
      gaatctttg atttacaca acactctca ggcagtgtt 
      ggtgttctt gtgccgttt acgtcacga gaagatcac 
      gattttttc caacatttg gaaatgcac atgaggagt 
      gaaaactca ccattgtgt ggaagagat cacctttca 
      tttagaagt tattatttt cctgtgaag aatgtcata 
      gatggagat ttatgtgaa cagtacaac tcgctggaa 
      cctgtcaaa caaaaaagc atagcgtcg gatttggac 
      cgtactcca gcggaagtt tcgaaaaaa ttagaagat 
      ataaggacc agatatgct ttctaa
    mRNA sequence
    • >TC010322 RA
      cttcaccgg tgtttgtgc gttattttg ccggtctgt 
      cgccatttg cagttagtt tttgtactt ttttgctaa 
      ataatgtat caaaacatt gcaaaaggt gctttatta 
      ttgtttttt aattaactt aaccatcga caaaaacgc 
      tcaaataaa taaccaagt caccacgtc acaacaatg 
      tgaaccatg cgaaaatcg aaaataaat ggttgaaaa 
      actttgaaa tattgaacg aaacgaagc tcatccacg 
      ttcaagata agcccccaa aatgtatct ctacaacct 
      gaccttaca aagggccac agccatcac ccacgccat 
      ccatggcaa tttttccgg caccaaaat gcaagaaat 
      tgtcatttc acgtggcaa aagcttgga agtgctacg 
      gcctgaccc caacaccgg taaagtcca cacgcttct 
      aaccgtcga aatattcgg agtcgtccg ctcgttgat 
      gtcgtttcg actcaccgg aggcaccaa agacttcgt 
      catcgtggg ctccgactc cggcagaat cgttattct 
      tgagtatat cccgtctaa aaacaactt cgagaaggt 
      ccaccagga gacgttcgg caaatcggg ggtcagacg 
      tattgttcc aggccagta cctagccac tgaccctcg 
      aggacgtgc tgtaatgat aggcgccgt cgagaaaca 
      aaaactcgc ttacatttt gaatagaga cgtgcaagc 
      ccgcttgac gatctcctc gcccctcga agcccacaa 
      aagcaacac tcttgtcta tcacatggt gggggtgga 
      cgtcggtta tgacaaccc tatgttcgc ttgcctcga 
      aatcgacta cgaagaagc agattcgga tccttctgg 
      cgaagcggc tcaaaaaac tcaacaaac cttgacctt 
      ctacgagct ggacctggg tgtaaatca cgttgtgcg 
      gaaatactc ggaaccgtt ggaggaaca cgcgaattt 
      cttagtcac ggttccagg gggcgacga tggcccttc 
      aggagtcct tatttgttc agagaatta tttgactta 
      taagaattt gggcgatca gcacgatat tcgttgccc 
      gattcccag aaggcggaa cgatttgga tgacccaga 
      gagaggcat gatttttgt ctgttctgc cacgcataa 
      gacgaagtc gatgttctt tttcctggc acagacgga 
      gcaagggga tatctttaa aatcactct cgaaacgga 
      tgaagatat ggtcacgga aatcaagtt aaagtattt 
      cgatactgt tccggtcgc ttccgctat gtgtgtgtt 
      gaagacggg gttcttgtt tgtaacttc ggaatttgg 
      caaccacta cttgtatca gatcgccca tcttggtga 
      cgacgacga tgagttgga gtttagctc cgctatgcc 
      cttagaaga aggtgacac ttttttttt cgcgcctcg 
      ttcccttcg taacttggt tttggtcga cgaaatgga 
      atcgctttc accgattct atcttgtcg agttgctga 
      tctggcagg tgaagacac tcctcaatt gtatatgct 
      ttgcggaag aggcccgag atcctcatt gcgggttct 
      acgacacgg tctcgaagt gtctgagat ggccgtgtc 
      cgagcttcc tggcaatcc caacgccgt ttggacggt 
      taagagaag aagtgacga tgaatatga tgcttatat 
      tattgtgtc tttcgttaa cgcaacttt ggtattgtc 
      aattggtga aactgtgga ggaggttac agacagtgg 
      ctttttggg tacgacacc aacattgtc ctgttcagc 
      tcttagtga cgatgcctt agttcaggt gtatccagg 
      cggtattcg gcatatttg ttccgataa gcgtgtaaa 
      tgagtggaa agctccagg gaagaaaac aatcgtaaa 
      gtgtgcaat taatcaacg acaagtggt tattgcact 
      gtcaggggg tgaactagc ttacttcga aatggatcc 
      gacgggcca attacacga atacaaaga acgaaaacg 
      tatgaacgc cgacgtcgt ttgcatggc attagcaaa 
      cgtggcgcc cggcgaaca attatccct cttccttgc 
      cgtgggatt agccgatag tacagtacg gattatctc 
      gctagatcc gagcgattg tttggcgcc gcgttctat 
      ccaaggatt acccgtttg tgccgagtc tttatgtat 
      cgtagaaat gggttgcac agatcgtga accggataa 
      cgctgcagc tgccagcac aacaagcac cttatatct 
      gaacattgg acttacaaa cggcgcttt acttcgcaa 
      cgtattaga tccagtttc tggagaatt gtcagacac 
      aagaacgag atatttggg ttcgagacc ggtcaaact 
      cttccgaat acgaatgca acaatcgga agctgttct 
      ggcaatgag tagccgttc ttggttgag ttattacta 
      ccaaagtcg gttttattt gactccact ctcgtatga 
      aagtctgga atatgcctc agggttcag ttccgagca 
      gtgtccaga aggcattgt tgcgatctc cacaaatac 
      gttacgtat tttggcgtt ggaaaaact aggggctgt 
      ttttaatca agtctcatt tccgcttga atacacccc 
      gaggaagtt tataatcca tcctgaaag caataattt 
      gctgatcat tgaaaccga gcacaatgc ctacaccga 
      agaaaccaa aaaacaacg aagattaca aatggccga 
      agaaatgaa agaagccgc aggtgaaga agaacaaga 
      gttggctaa agaaatggc cgatgcgtt tttgaatga 
      agacttgcc cgaaagtat tttttcggc gcctaaagc 
      cgggcatgg aatgtgggc ttcgacgct taaaataat 
      ggatccggt acaaggaat cgtgcataa gtgtatacg 
      attggaaca gaacgaggc agcaatgtc tagtgtttt 
      ggttaaatt ccaaaatca gccacaaca aactctgtt 
      cttgattgt tggcatttc taaagattt tcaactaaa 
      tccaaggca ttgtaacac tgggttttt ggatactta 
      taaaatgga tccaatggg tcgggagtt ggaatttgt 
      tcatagaac tcctgttga tgaagttcc aatggccct 
      ttgtgctta taatggact gctacttgc aggagttgg 
      caggatgtt gaggcttta cgatatggg gaagaagaa 
      actcttaag gaaatgtga aaacaagca cataccaaa 
      cgctattgt gaatattca agccatggg caaacgaat 
      tttcgtttc ggacgttca agagtctgt tttcatggt 
      tcggtacaa gagagctga aaatcagtt gattatatt 
      tgccgatga cacgcaccc tcgttgggt tacttgcaa 
      ctgtgtgct tgattatga tacagtggc tgttgccga 
      caagtttgg taatattgc aattttacg tttgccacc 
      aaatgtaag tgatgatgt tgaagagga tcctactgg 
      acataaatc gttatggga tagaggact gctaaatgg 
      ggcgtcaca aaaagctga tactattgc aacattcca 
      cgttggtga gactgtcac gtggttgca aaaagccac 
      actgattcc tggaggttg ggaatcttt gatttacac 
      aacactctc aggcagtgt tggtgttct tgtgccgtt 
      tacgtcacg agaagatca cgatttttt ccaacattt 
      ggaaatgca catgaggag tgaaaactc accattgtg 
      tggaagaga tcacctttc atttagaag ttattattt 
      tcctgtgaa gaatgtcat agatggaga tttatgtga 
      acagtacaa ctcgctgga acctgtcaa acaaaaaag 
      catagcgtc ggatttgga ccgtactcc agcggaagt 
      ttcgaaaaa attagaaga tataaggac cagatatgc 
      tttctaagt tatttttta ctgaattgt atttttgct 
      tcaaagaat taataaatg tcgttctgc gtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY