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Gene information
General information
TC010318 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451814.1: 1834..11333
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010318
    iBeetle screen iB_01665

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 5

    vitellogenic egg chamber mostlynot present - Number of animals showing the phenotype: 5
    vitellarium circumference decreased - Number of animals showing the phenotype: 5
    tropharium shape irregular - Number of animals showing the phenotype: 5
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010318 RA
      MSSQEDIIK KQKTCMQVA TNVTEQRHH VSRAKRGQI 
      LGHLRGFRG CTVWFTGLS GAGKTSIAF ELEAYLIQH 
      GIPAYGLDG DNMRTGLNK DLGFSKQDR EENIRRVAE 
      VARLFADAG QICLCSFVS PFAEDRELA RRIHRDNDL 
      PFFEVFVDC PLPVCEQRD TKGLYHKAR QGAIKGFTG 
      IDQPYEKPE HPDLVVKTI ESSVEESMF HVVEMLQEN 
      GIIPLVEED YEKVLELFV PKDRLAAAN EEAATLPKL 
      NLTVLDLQW LQVLSEGWA SPLKGFMRE DQFLQTLHF 
      NCLLDEVKN TNQSIPIVL PVSSADKER LDGASALSL 
      YHNGICYAI LRKPEFYYH RKEERVARQ FGITNKDHP 
      YIKMVYESG DWLVGGELE VLKRVQWGD ELDHYRLTP 
      NELRRKFKL MGADAVFAF QLRNPIHNG HALLMQDTR 
      KQLKERGYR KPVLLLHPL GGWTKDDDV PLHTRLLQH 
      QAVLDEGLL DRFSTVLAI FPSPMMYAG PTEVQWHAK 
      ARMNAGANF YIVGRDPAG VPHPRGKDA TPDGNLYDV 
      THGARVLKM APGLTSLEI IPFRVAAYD CKNKKMAFF 
      DPARKEEFE FISGTKMRT LARNGDNPP DGFMAPKAW 
      RVLADYYQS LIKDKY
    CDS sequence
    • >TC010318 RA
      atgtcgagc caagaagac atcattaag aagcagaag 
      acgtgcatg caagtggcc accaacgtc acggagcag 
      cggcaccac gtctcccga gccaagcgc ggccagatt 
      ttggggcac ctgcggggc ttccggggc tgcactgtc 
      tggttcacg gggctgtct ggcgcaggt aaaacctcc 
      atagccttc gaactggag gcctacctc atccagcac 
      ggcatcccc gcctacggc ctggacggc gacaacatg 
      cgcacgggg ctcaacaag gacctgggg ttcagcaaa 
      caggaccgg gaggagaac ataagacgc gtggcggaa 
      gtggcgcga cttttcgca gacgctggc cagatctgt 
      ctttgtagc ttcgtgtcc ccatttgca gaagacagg 
      gagctggcc cggaggata caccgcgat aacgacctc 
      ccgttcttc gaagtgttt gtcgattgt ccgctgccg 
      gtgtgcgag cagagggac accaagggg ctgtaccac 
      aaggccagg cagggggct atcaagggg ttcacgggg 
      attgaccag ccgtacgag aagccagag catcccgat 
      ttggtcgtg aagacgatt gagtcgtcg gtggaggag 
      tcgatgttc cacgttgtg gagatgttg caagaaaat 
      gggattatt cccctggtg gaggaggat tacgagaag 
      gtgctggag ctgtttgtg cccaaggac cggcttgct 
      gccgccaat gaggaggcg gcgactttg cccaagttg 
      aatttgact gtcctggat ttgcagtgg ctgcaggtt 
      ttgagcgaa ggctgggcc tccccctta aaaggcttc 
      atgcgcgaa gaccaattc ctgcaaacc ctccacttc 
      aactgcctc ctcgacgaa gtgaaaaac actaaccaa 
      tcaatcccc atcgtcttg cccgtctca tccgccgac 
      aaagagcgc ctcgacggc gcctcggcc ctatctctc 
      taccacaac ggcatctgc tacgccatt ctgcgcaaa 
      cccgaattc tactaccac cgcaaggaa gaacgcgtc 
      gcccgccaa ttcggcatc accaacaaa gaccaccct 
      tacataaaa atggtctac gaatcgggc gactggctc 
      gtggggggc gaactcgaa gtcctgaaa cgcgtgcaa 
      tggggcgac gaactcgac cactaccga ctcaccccc 
      aacgaactc cgccgaaag ttcaagtta atgggggct 
      gacgcagtt ttcgccttc caactgcgc aaccccatc 
      cacaacggt cacgcgctg ctgatgcaa gacacgcgg 
      aaacagctg aaagaaagg ggctaccgc aagccggtg 
      ctgcttttg caccctctg ggtggttgg acgaaagac 
      gacgatgtt ccccttcac actagattg ctccaacac 
      caagctgtg ctagatgag ggcttgctt gatcggttc 
      tcgaccgtt ctggccatt ttcccgagt cccatgatg 
      tacgcggga cccactgaa gtgcagtgg catgccaag 
      gcgcggatg aacgcgggc gcgaatttc tatatcgtt 
      gggagagat cccgcagga gtgccgcac ccacggggg 
      aaggacgcg actccagat gggaacttg tacgatgtc 
      acccatggg gctcgtgtg ctgaagatg gcacctggg 
      ttaacctcg cttgagatc attcccttc cgggtggcc 
      gcctacgat tgcaagaac aagaaaatg gccttcttt 
      gatccggcc aggaaggag gagtttgag ttcatttcg 
      gggacgaag atgaggacg cttgcgcgt aatggggat 
      aacccaccg gatgggttt atggcgccc aaggcctgg 
      agggtgctt gccgattac taccagtct ttgattaaa 
      gataaatat tag
    mRNA sequence
    • >TC010318 RA
      tggcaaccc aacctcaag gtaaataaa gtaaacata 
      acctagatc ccgcgaact gtcagtgtc agactgaag 
      agtagtgtg aaagaggaa ggaagtaat cggtttcac 
      ttccattac ctggccgat gtgctactt tgaaagtga 
      aatccgaaa gttgttgta aacctgtcg ttctaaacg 
      cactgccat gtcgagcca agaagacat cattaagaa 
      gcagaagac gtgcatgca agtggccac caacgtcac 
      ggagcagcg gcaccacgt ctcccgagc caagcgcgg 
      ccagatttt ggggcacct gcggggctt ccggggctg 
      cactgtctg gttcacggg gctgtctgg cgcaggtaa 
      aacctccat agccttcga actggaggc ctacctcat 
      ccagcacgg catccccgc ctacggcct ggacggcga 
      caacatgcg cacggggct caacaagga cctggggtt 
      cagcaaaca ggaccggga ggagaacat aagacgcgt 
      ggcggaagt ggcgcgact tttcgcaga cgctggcca 
      gatctgtct ttgtagctt cgtgtcccc atttgcaga 
      agacaggga gctggcccg gaggataca ccgcgataa 
      cgacctccc gttcttcga agtgtttgt cgattgtcc 
      gctgccggt gtgcgagca gagggacac caaggggct 
      gtaccacaa ggccaggca gggggctat caaggggtt 
      cacggggat tgaccagcc gtacgagaa gccagagca 
      tcccgattt ggtcgtgaa gacgattga gtcgtcggt 
      ggaggagtc gatgttcca cgttgtgga gatgttgca 
      agaaaatgg gattattcc cctggtgga ggaggatta 
      cgagaaggt gctggagct gtttgtgcc caaggaccg 
      gcttgctgc cgccaatga ggaggcggc gactttgcc 
      caagttgaa tttgactgt cctggattt gcagtggct 
      gcaggtttt gagcgaagg ctgggcctc ccccttaaa 
      aggcttcat gcgcgaaga ccaattcct gcaaaccct 
      ccacttcaa ctgcctcct cgacgaagt gaaaaacac 
      taaccaatc aatccccat cgtcttgcc cgtctcatc 
      cgccgacaa agagcgcct cgacggcgc ctcggccct 
      atctctcta ccacaacgg catctgcta cgccattct 
      gcgcaaacc cgaattcta ctaccaccg caaggaaga 
      acgcgtcgc ccgccaatt cggcatcac caacaaaga 
      ccaccctta cataaaaat ggtctacga atcgggcga 
      ctggctcgt ggggggcga actcgaagt cctgaaacg 
      cgtgcaatg gggcgacga actcgacca ctaccgact 
      cacccccaa cgaactccg ccgaaagtt caagttaat 
      gggggctga cgcagtttt cgccttcca actgcgcaa 
      ccccatcca caacggtca cgcgctgct gatgcaaga 
      cacgcggaa acagctgaa agaaagggg ctaccgcaa 
      gccggtgct gcttttgca ccctctggg tggttggac 
      gaaagacga cgatgttcc ccttcacac tagattgct 
      ccaacacca agctgtgct agatgaggg cttgcttga 
      tcggttctc gaccgttct ggccatttt cccgagtcc 
      catgatgta cgcgggacc cactgaagt gcagtggca 
      tgccaaggc gcggatgaa cgcgggcgc gaatttcta 
      tatcgttgg gagagatcc cgcaggagt gccgcaccc 
      acgggggaa ggacgcgac tccagatgg gaacttgta 
      cgatgtcac ccatggggc tcgtgtgct gaagatggc 
      acctgggtt aacctcgct tgagatcat tcccttccg 
      ggtggccgc ctacgattg caagaacaa gaaaatggc 
      cttctttga tccggccag gaaggagga gtttgagtt 
      catttcggg gacgaagat gaggacgct tgcgcgtaa 
      tggggataa cccaccgga tgggtttat ggcgcccaa 
      ggcctggag ggtgcttgc cgattacta ccagtcttt 
      gattaaaga taaatatta gatcgaaca ttccttctt 
      ttctttatg tctcgactt tttcgaact cgattttat 
      cttttcgaa gtctttcct tctgtaata ttattttat 
      tattggttt taattgtgc actataatc gtaggtgta 
      aaggaattt gttttagct ttaagtttc aattgcaat 
      tgatgagct tttaaaaat tgctaaatt gtttagaag 
      attttatta tctacttaa atgcatttc agaaggtaa 
      tgcaatgta tttcttaat gttctagcg aggacatga 
      agttgtaaa atgcataca ggactcaat atttccttt 
      taatctatt gaggtctta tattccaaa cttttacat 
      gaattttta acggcatag tccatagct ttatatcat 
      gtatgtgtt atgttttaa atatatttt attttattt 
      t
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