Status
Please wait ...
Gene information
General information
TC010300 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452189.1: 2335..6229
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010300
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01663

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010300 RA
      MATRSKPGG KHDDQSVET LAEVFRCFI CMEKLRDAH 
      LCPHCSKLC CYVCIRRWL TEQRSQCPH CRASLHLHE 
      LVNCRWVEE VTQQLDTLQ AVNLNSSRG EDNDRDKCA 
      THSEKLSVY CWTCRCCIC HQCALWGGT HSGHTFKPL 
      DEVYEQHVT QIKDEVVQL RRRLMELIS IVQEVERNV 
      ESVRSAKDE RVREIRNAV ELMIARLDS QLKTKLLTL 
      MGQKDSLTQ ETEQLEHLL HEIEHQLHT CTRSEMISK 
      SSELSRMIH AIRKKPMTS FVTAPVPAD FHSEIVPAY 
      DSSTFVMHC FSQLQRKAD PVYSTPLHC NGLCWRLKV 
      YPDGNGVVR GNYLSVFLE LSAGYPETS KYEYRVEMI 
      HQASRDSSK NIVREFASD FEVGECWGY NRFFRLDLL 
      ASEGYLNVA SDTLVLRFQ VRAPTFYQR CRDQQWYIN 
      QLQTVQNQY ISQINESKE RLAAEISRN AVAATLGAA 
      TQIPDIATI GMVKSMYNM APVVPSEAA EAQPMPSTS 
      KNGVAEKFD ITTLAKNLM SNVNTKQVA TTTATGDCP 
      NNTSGSSST SSIQTKKRE TGRQSGNES ATETPRHLL 
      GLGVSLSSP NLLNSNVSF TLCCSSSES DLSEPENLM 
      EEFEPAEAN LISTGDNSN DENDVDDET MSGENDVEY 
      AEFSMTQRL MMRDSSPAA AGATALSPT EGNSFEDKL 
      MLLQLFDRL ENSPQATTA PNVPQTDLP PVNLDLVLL 
      DNLSIKSDD QLSSPTVPD VANRRKLRK RFKGGASSE 
      GGATANPTW TETVPLNTK ESVMGPLES FLRSINCYP 
      EHDQHRKKH SSKRKPLKG AERILGFSL PQPASPPPL 
      GDNVDGSVQ PSSSNGFSW TPSLLSQRR PNKVKLQTD 
      LSKPEKESK NGRSDNQPH SA
    CDS sequence
    • >TC010300 RA
      atggccact aggagcaaa ccaggaggc aaacatgac 
      gaccaaagc gtggagacc ctggccgag gtcttccgc 
      tgcttcatc tgcatggag aaactgcgc gacgcccat 
      ctgtgcccg cactgctcc aagctgtgc tgctacgtt 
      tgcatccgg cggtggctc accgaacag cggtcgcag 
      tgcccccat tgccgggcg tcgctgcat ctgcacgaa 
      ctggtcaac tgcagatgg gtggaggag gtcacccag 
      cagctcgac accctgcag gccgtcaac ttgaacagc 
      agcaggggc gaagacaat gaccgggac aagtgtgcc 
      acacattcg gagaaactg tccgtctat tgctggacg 
      tgccgctgc tgcatttgc caccagtgc gctctgtgg 
      ggcggcact cactcgggg cacaccttc aagccactc 
      gacgaagtc tacgaacag cacgtcacc cagattaag 
      gacgaggtg gttcagctc aggcgccgc ctgatggag 
      ctgatcagc atcgtgcag gaagttgaa cgtaacgtg 
      gaatcggtt cgttcggcg aaagacgag cgcgtccgt 
      gagatccgc aacgccgtc gagctgatg attgcccgt 
      ctcgactcg cagttgaag accaaactc ctgactttg 
      atgggccag aaggactcg ctcacgcaa gaaaccgag 
      cagttggag cacctcctg cacgagatc gagcaccag 
      ctgcacacg tgcacccgc tcggagatg atctcgaag 
      agttcggaa ctgtcccga atgatccat gccattagg 
      aaaaaacca atgacgagt ttcgtgaca gctccagtt 
      cctgcggat tttcacagc gaaattgtg ccggcttac 
      gacagcagc acattcgta atgcattgt ttttcccaa 
      ctgcaacga aaagccgac cctgtctac tccactccc 
      ttgcactgt aacgggctc tgttggcgc ctcaaagtc 
      tacccggac ggcaacggt gttgtccgt ggcaattac 
      ctatcagtt ttcctcgaa ctgagcgcc ggttatccc 
      gaaacctcg aaatatgag taccgggtc gagatgatc 
      catcaggcg agtcgcgac tcgtcgaaa aacatcgtt 
      cgggaattc gcgtccgat ttcgaagtt ggcgagtgt 
      tggggctat aaccggttc ttccgactg gacctcctg 
      gccagcgag ggctacctg aacgtagcc tcggacacg 
      ctcgttttg cggttccag gtgcgcgcc cccaccttc 
      taccagcgc tgccgcgac cagcagtgg tacatcaac 
      caactgcaa accgttcaa aaccagtac atttcacaa 
      atcaacgaa tccaaagag cgcctcgcc gccgaaatc 
      tcccgaaat gccgttgcg gcgactctg ggggccgcc 
      acgcaaatc cccgacatt gccacaatt ggcatggtc 
      aaaagcatg tacaacatg gctccagtg gtgccatct 
      gaggcggcc gaggcccaa ccaatgccg tcaacttcg 
      aaaaatggg gttgcggaa aaattcgat atcacgact 
      ttggccaag aatttaatg agcaatgtt aatacgaag 
      caagtggcg actacgacg gcaacgggc gattgtccg 
      aataatacg agtggttcg tcgtcgacg agttccatc 
      cagacgaaa aaaagggaa acggggcga caaagtggc 
      aacgaatcg gccactgaa actccgcga catttactg 
      gggctggga gtgtcgctc agttcgccg aatttgttg 
      aattcgaac gtttccttc acgctttgt tgcagcagc 
      agcgaaagc gacctgagc gagccggag aacctgatg 
      gaggagttc gagccggcc gaggccaac ttgatcagc 
      actggcgac aattcgaac gatgagaac gacgtcgac 
      gacgagaca atgtcagga gagaatgat gtagagtat 
      gcggaattt tcaatgacc cagcgcctc atgatgcgg 
      gattcgagt cctgcggcc gcaggagcc acggctctc 
      tcgcccacc gagggcaac tcatttgag gacaaactg 
      atgctcttg caactgttt gatagattg gaaaattcg 
      ccacaggcg acaacggca cctaacgtg cctcagacc 
      gacttgcca cctgtcaat ctcgatcta gttctctta 
      gacaaccta tcaatcaag agcgatgat cagttatca 
      tcgcccact gtacctgac gtggccaat cgacgaaaa 
      ctgcggaag cggttcaaa gggggcgcc agcagtgag 
      ggtggcgct acagctaac ccgacttgg actgagacc 
      gtgccattg aacacgaag gagtcagtg atggggcct 
      ttggagtcc ttcctgcgt tccattaat tgttatccc 
      gaacatgac caacatcgc aagaaacac tcttcgaag 
      cggaaaccg ctaaaaggc gccgaacgc atccttggt 
      ttctcgctg ccacaaccg gcgtcgcct ccaccgctc 
      ggcgacaac gtcgatggt agtgtccaa ccatcgtca 
      tcgaacggt ttcagctgg acaccgtct ctcttaagt 
      cagaggcgc cccaataaa gtcaaactg caaactgat 
      ctcagcaaa ccggaaaag gagtccaaa aacggaagg 
      tctgataac cagccgcat tccgcctga
    mRNA sequence
    • >TC010300 RA
      tttgacaag acagctgtc aaaaaccag ttgattgcc 
      cccgcacga aatcctaac tgtattttc ctttgttcg 
      ctgtatttt gaacagtgg aatgttaaa tggggacgt 
      cggcgtaaa gcaacaatg ctagtttga cccgatcgc 
      tcaccttaa cgctcccca aaaaagtgt accttcggt 
      gtgcaaaat ggccactag gagcaaacc aggaggcaa 
      acatgacga ccaaagcgt ggagaccct ggccgaggt 
      cttccgctg cttcatctg catggagaa actgcgcga 
      cgcccatct gtgcccgca ctgctccaa gctgtgctg 
      ctacgtttg catccggcg gtggctcac cgaacagcg 
      gtcgcagtg cccccattg ccgggcgtc gctgcatct 
      gcacgaact ggtcaactg cagatgggt ggaggaggt 
      cacccagca gctcgacac cctgcaggc cgtcaactt 
      gaacagcag caggggcga agacaatga ccgggacaa 
      gtgtgccac acattcgga gaaactgtc cgtctattg 
      ctggacgtg ccgctgctg catttgcca ccagtgcgc 
      tctgtgggg cggcactca ctcggggca caccttcaa 
      gccactcga cgaagtcta cgaacagca cgtcaccca 
      gattaagga cgaggtggt tcagctcag gcgccgcct 
      gatggagct gatcagcat cgtgcagga agttgaacg 
      taacgtgga atcggttcg ttcggcgaa agacgagcg 
      cgtccgtga gatccgcaa cgccgtcga gctgatgat 
      tgcccgtct cgactcgca gttgaagac caaactcct 
      gactttgat gggccagaa ggactcgct cacgcaaga 
      aaccgagca gttggagca cctcctgca cgagatcga 
      gcaccagct gcacacgtg cacccgctc ggagatgat 
      ctcgaagag ttcggaact gtcccgaat gatccatgc 
      cattaggaa aaaaccaat gacgagttt cgtgacagc 
      tccagttcc tgcggattt tcacagcga aattgtgcc 
      ggcttacga cagcagcac attcgtaat gcattgttt 
      ttcccaact gcaacgaaa agccgaccc tgtctactc 
      cactccctt gcactgtaa cgggctctg ttggcgcct 
      caaagtcta cccggacgg caacggtgt tgtccgtgg 
      caattacct atcagtttt cctcgaact gagcgccgg 
      ttatcccga aacctcgaa atatgagta ccgggtcga 
      gatgatcca tcaggcgag tcgcgactc gtcgaaaaa 
      catcgttcg ggaattcgc gtccgattt cgaagttgg 
      cgagtgttg gggctataa ccggttctt ccgactgga 
      cctcctggc cagcgaggg ctacctgaa cgtagcctc 
      ggacacgct cgttttgcg gttccaggt gcgcgcccc 
      caccttcta ccagcgctg ccgcgacca gcagtggta 
      catcaacca actgcaaac cgttcaaaa ccagtacat 
      ttcacaaat caacgaatc caaagagcg cctcgccgc 
      cgaaatctc ccgaaatgc cgttgcggc gactctggg 
      ggccgccac gcaaatccc cgacattgc cacaattgg 
      catggtcaa aagcatgta caacatggc tccagtggt 
      gccatctga ggcggccga ggcccaacc aatgccgtc 
      aacttcgaa aaatggggt tgcggaaaa attcgatat 
      cacgacttt ggccaagaa tttaatgag caatgttaa 
      tacgaagca agtggcgac tacgacggc aacgggcga 
      ttgtccgaa taatacgag tggttcgtc gtcgacgag 
      ttccatcca gacgaaaaa aagggaaac ggggcgaca 
      aagtggcaa cgaatcggc cactgaaac tccgcgaca 
      tttactggg gctgggagt gtcgctcag ttcgccgaa 
      tttgttgaa ttcgaacgt ttccttcac gctttgttg 
      cagcagcag cgaaagcga cctgagcga gccggagaa 
      cctgatgga ggagttcga gccggccga ggccaactt 
      gatcagcac tggcgacaa ttcgaacga tgagaacga 
      cgtcgacga cgagacaat gtcaggaga gaatgatgt 
      agagtatgc ggaattttc aatgaccca gcgcctcat 
      gatgcggga ttcgagtcc tgcggccgc aggagccac 
      ggctctctc gcccaccga gggcaactc atttgagga 
      caaactgat gctcttgca actgtttga tagattgga 
      aaattcgcc acaggcgac aacggcacc taacgtgcc 
      tcagaccga cttgccacc tgtcaatct cgatctagt 
      tctcttaga caacctatc aatcaagag cgatgatca 
      gttatcatc gcccactgt acctgacgt ggccaatcg 
      acgaaaact gcggaagcg gttcaaagg gggcgccag 
      cagtgaggg tggcgctac agctaaccc gacttggac 
      tgagaccgt gccattgaa cacgaagga gtcagtgat 
      ggggccttt ggagtcctt cctgcgttc cattaattg 
      ttatcccga acatgacca acatcgcaa gaaacactc 
      ttcgaagcg gaaaccgct aaaaggcgc cgaacgcat 
      ccttggttt ctcgctgcc acaaccggc gtcgcctcc 
      accgctcgg cgacaacgt cgatggtag tgtccaacc 
      atcgtcatc gaacggttt cagctggac accgtctct 
      cttaagtca gaggcgccc caataaagt caaactgca 
      aactgatct cagcaaacc ggaaaagga gtccaaaaa 
      cggaaggtc tgataacca gccgcattc cgcctgatc 
      tgtgtaact tatttatta tgattaatt taatttttt 
      acgtggtag tttggtgga ctaatacag tacttaatg 
      attcaatta atccatcat tccagtagg aattaaaaa 
      ctaactaaa attaggctg tgtgagttg tcagctttg 
      aatgtctct aaataaaaa att
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY