Status
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Gene information
General information
TC010300 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452189.1: 2335..6229
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010300
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01663

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010300 RA
      MATRSKPGG KHDDQSVET LAEVFRCFI CMEKLRDAH 
LCPHCSKLC CYVCIRRWL TEQRSQCPH CRASLHLHE 
LVNCRWVEE VTQQLDTLQ AVNLNSSRG EDNDRDKCA 
THSEKLSVY CWTCRCCIC HQCALWGGT HSGHTFKPL 
DEVYEQHVT QIKDEVVQL RRRLMELIS IVQEVERNV 
ESVRSAKDE RVREIRNAV ELMIARLDS QLKTKLLTL 
MGQKDSLTQ ETEQLEHLL HEIEHQLHT CTRSEMISK 
SSELSRMIH AIRKKPMTS FVTAPVPAD FHSEIVPAY 
DSSTFVMHC FSQLQRKAD PVYSTPLHC NGLCWRLKV 
YPDGNGVVR GNYLSVFLE LSAGYPETS KYEYRVEMI 
HQASRDSSK NIVREFASD FEVGECWGY NRFFRLDLL 
ASEGYLNVA SDTLVLRFQ VRAPTFYQR CRDQQWYIN 
QLQTVQNQY ISQINESKE RLAAEISRN AVAATLGAA 
TQIPDIATI GMVKSMYNM APVVPSEAA EAQPMPSTS 
KNGVAEKFD ITTLAKNLM SNVNTKQVA TTTATGDCP 
NNTSGSSST SSIQTKKRE TGRQSGNES ATETPRHLL 
GLGVSLSSP NLLNSNVSF TLCCSSSES DLSEPENLM 
EEFEPAEAN LISTGDNSN DENDVDDET MSGENDVEY 
AEFSMTQRL MMRDSSPAA AGATALSPT EGNSFEDKL 
MLLQLFDRL ENSPQATTA PNVPQTDLP PVNLDLVLL 
DNLSIKSDD QLSSPTVPD VANRRKLRK RFKGGASSE 
GGATANPTW TETVPLNTK ESVMGPLES FLRSINCYP 
EHDQHRKKH SSKRKPLKG AERILGFSL PQPASPPPL 
GDNVDGSVQ PSSSNGFSW TPSLLSQRR PNKVKLQTD 
LSKPEKESK NGRSDNQPH SA
    CDS sequence
    • >TC010300 RA
      atggccact aggagcaaa ccaggaggc aaacatgac 
gaccaaagc gtggagacc ctggccgag gtcttccgc 
tgcttcatc tgcatggag aaactgcgc gacgcccat 
ctgtgcccg cactgctcc aagctgtgc tgctacgtt 
tgcatccgg cggtggctc accgaacag cggtcgcag 
tgcccccat tgccgggcg tcgctgcat ctgcacgaa 
ctggtcaac tgcagatgg gtggaggag gtcacccag 
cagctcgac accctgcag gccgtcaac ttgaacagc 
agcaggggc gaagacaat gaccgggac aagtgtgcc 
acacattcg gagaaactg tccgtctat tgctggacg 
tgccgctgc tgcatttgc caccagtgc gctctgtgg 
ggcggcact cactcgggg cacaccttc aagccactc 
gacgaagtc tacgaacag cacgtcacc cagattaag 
gacgaggtg gttcagctc aggcgccgc ctgatggag 
ctgatcagc atcgtgcag gaagttgaa cgtaacgtg 
gaatcggtt cgttcggcg aaagacgag cgcgtccgt 
gagatccgc aacgccgtc gagctgatg attgcccgt 
ctcgactcg cagttgaag accaaactc ctgactttg 
atgggccag aaggactcg ctcacgcaa gaaaccgag 
cagttggag cacctcctg cacgagatc gagcaccag 
ctgcacacg tgcacccgc tcggagatg atctcgaag 
agttcggaa ctgtcccga atgatccat gccattagg 
aaaaaacca atgacgagt ttcgtgaca gctccagtt 
cctgcggat tttcacagc gaaattgtg ccggcttac 
gacagcagc acattcgta atgcattgt ttttcccaa 
ctgcaacga aaagccgac cctgtctac tccactccc 
ttgcactgt aacgggctc tgttggcgc ctcaaagtc 
tacccggac ggcaacggt gttgtccgt ggcaattac 
ctatcagtt ttcctcgaa ctgagcgcc ggttatccc 
gaaacctcg aaatatgag taccgggtc gagatgatc 
catcaggcg agtcgcgac tcgtcgaaa aacatcgtt 
cgggaattc gcgtccgat ttcgaagtt ggcgagtgt 
tggggctat aaccggttc ttccgactg gacctcctg 
gccagcgag ggctacctg aacgtagcc tcggacacg 
ctcgttttg cggttccag gtgcgcgcc cccaccttc 
taccagcgc tgccgcgac cagcagtgg tacatcaac 
caactgcaa accgttcaa aaccagtac atttcacaa 
atcaacgaa tccaaagag cgcctcgcc gccgaaatc 
tcccgaaat gccgttgcg gcgactctg ggggccgcc 
acgcaaatc cccgacatt gccacaatt ggcatggtc 
aaaagcatg tacaacatg gctccagtg gtgccatct 
gaggcggcc gaggcccaa ccaatgccg tcaacttcg 
aaaaatggg gttgcggaa aaattcgat atcacgact 
ttggccaag aatttaatg agcaatgtt aatacgaag 
caagtggcg actacgacg gcaacgggc gattgtccg 
aataatacg agtggttcg tcgtcgacg agttccatc 
cagacgaaa aaaagggaa acggggcga caaagtggc 
aacgaatcg gccactgaa actccgcga catttactg 
gggctggga gtgtcgctc agttcgccg aatttgttg 
aattcgaac gtttccttc acgctttgt tgcagcagc 
agcgaaagc gacctgagc gagccggag aacctgatg 
gaggagttc gagccggcc gaggccaac ttgatcagc 
actggcgac aattcgaac gatgagaac gacgtcgac 
gacgagaca atgtcagga gagaatgat gtagagtat 
gcggaattt tcaatgacc cagcgcctc atgatgcgg 
gattcgagt cctgcggcc gcaggagcc acggctctc 
tcgcccacc gagggcaac tcatttgag gacaaactg 
atgctcttg caactgttt gatagattg gaaaattcg 
ccacaggcg acaacggca cctaacgtg cctcagacc 
gacttgcca cctgtcaat ctcgatcta gttctctta 
gacaaccta tcaatcaag agcgatgat cagttatca 
tcgcccact gtacctgac gtggccaat cgacgaaaa 
ctgcggaag cggttcaaa gggggcgcc agcagtgag 
ggtggcgct acagctaac ccgacttgg actgagacc 
gtgccattg aacacgaag gagtcagtg atggggcct 
ttggagtcc ttcctgcgt tccattaat tgttatccc 
gaacatgac caacatcgc aagaaacac tcttcgaag 
cggaaaccg ctaaaaggc gccgaacgc atccttggt 
ttctcgctg ccacaaccg gcgtcgcct ccaccgctc 
ggcgacaac gtcgatggt agtgtccaa ccatcgtca 
tcgaacggt ttcagctgg acaccgtct ctcttaagt 
cagaggcgc cccaataaa gtcaaactg caaactgat 
ctcagcaaa ccggaaaag gagtccaaa aacggaagg 
tctgataac cagccgcat tccgcctga
    mRNA sequence
    • >TC010300 RA
      tttgacaag acagctgtc aaaaaccag ttgattgcc 
cccgcacga aatcctaac tgtattttc ctttgttcg 
ctgtatttt gaacagtgg aatgttaaa tggggacgt 
cggcgtaaa gcaacaatg ctagtttga cccgatcgc 
tcaccttaa cgctcccca aaaaagtgt accttcggt 
gtgcaaaat ggccactag gagcaaacc aggaggcaa 
acatgacga ccaaagcgt ggagaccct ggccgaggt 
cttccgctg cttcatctg catggagaa actgcgcga 
cgcccatct gtgcccgca ctgctccaa gctgtgctg 
ctacgtttg catccggcg gtggctcac cgaacagcg 
gtcgcagtg cccccattg ccgggcgtc gctgcatct 
gcacgaact ggtcaactg cagatgggt ggaggaggt 
cacccagca gctcgacac cctgcaggc cgtcaactt 
gaacagcag caggggcga agacaatga ccgggacaa 
gtgtgccac acattcgga gaaactgtc cgtctattg 
ctggacgtg ccgctgctg catttgcca ccagtgcgc 
tctgtgggg cggcactca ctcggggca caccttcaa 
gccactcga cgaagtcta cgaacagca cgtcaccca 
gattaagga cgaggtggt tcagctcag gcgccgcct 
gatggagct gatcagcat cgtgcagga agttgaacg 
taacgtgga atcggttcg ttcggcgaa agacgagcg 
cgtccgtga gatccgcaa cgccgtcga gctgatgat 
tgcccgtct cgactcgca gttgaagac caaactcct 
gactttgat gggccagaa ggactcgct cacgcaaga 
aaccgagca gttggagca cctcctgca cgagatcga 
gcaccagct gcacacgtg cacccgctc ggagatgat 
ctcgaagag ttcggaact gtcccgaat gatccatgc 
cattaggaa aaaaccaat gacgagttt cgtgacagc 
tccagttcc tgcggattt tcacagcga aattgtgcc 
ggcttacga cagcagcac attcgtaat gcattgttt 
ttcccaact gcaacgaaa agccgaccc tgtctactc 
cactccctt gcactgtaa cgggctctg ttggcgcct 
caaagtcta cccggacgg caacggtgt tgtccgtgg 
caattacct atcagtttt cctcgaact gagcgccgg 
ttatcccga aacctcgaa atatgagta ccgggtcga 
gatgatcca tcaggcgag tcgcgactc gtcgaaaaa 
catcgttcg ggaattcgc gtccgattt cgaagttgg 
cgagtgttg gggctataa ccggttctt ccgactgga 
cctcctggc cagcgaggg ctacctgaa cgtagcctc 
ggacacgct cgttttgcg gttccaggt gcgcgcccc 
caccttcta ccagcgctg ccgcgacca gcagtggta 
catcaacca actgcaaac cgttcaaaa ccagtacat 
ttcacaaat caacgaatc caaagagcg cctcgccgc 
cgaaatctc ccgaaatgc cgttgcggc gactctggg 
ggccgccac gcaaatccc cgacattgc cacaattgg 
catggtcaa aagcatgta caacatggc tccagtggt 
gccatctga ggcggccga ggcccaacc aatgccgtc 
aacttcgaa aaatggggt tgcggaaaa attcgatat 
cacgacttt ggccaagaa tttaatgag caatgttaa 
tacgaagca agtggcgac tacgacggc aacgggcga 
ttgtccgaa taatacgag tggttcgtc gtcgacgag 
ttccatcca gacgaaaaa aagggaaac ggggcgaca 
aagtggcaa cgaatcggc cactgaaac tccgcgaca 
tttactggg gctgggagt gtcgctcag ttcgccgaa 
tttgttgaa ttcgaacgt ttccttcac gctttgttg 
cagcagcag cgaaagcga cctgagcga gccggagaa 
cctgatgga ggagttcga gccggccga ggccaactt 
gatcagcac tggcgacaa ttcgaacga tgagaacga 
cgtcgacga cgagacaat gtcaggaga gaatgatgt 
agagtatgc ggaattttc aatgaccca gcgcctcat 
gatgcggga ttcgagtcc tgcggccgc aggagccac 
ggctctctc gcccaccga gggcaactc atttgagga 
caaactgat gctcttgca actgtttga tagattgga 
aaattcgcc acaggcgac aacggcacc taacgtgcc 
tcagaccga cttgccacc tgtcaatct cgatctagt 
tctcttaga caacctatc aatcaagag cgatgatca 
gttatcatc gcccactgt acctgacgt ggccaatcg 
acgaaaact gcggaagcg gttcaaagg gggcgccag 
cagtgaggg tggcgctac agctaaccc gacttggac 
tgagaccgt gccattgaa cacgaagga gtcagtgat 
ggggccttt ggagtcctt cctgcgttc cattaattg 
ttatcccga acatgacca acatcgcaa gaaacactc 
ttcgaagcg gaaaccgct aaaaggcgc cgaacgcat 
ccttggttt ctcgctgcc acaaccggc gtcgcctcc 
accgctcgg cgacaacgt cgatggtag tgtccaacc 
atcgtcatc gaacggttt cagctggac accgtctct 
cttaagtca gaggcgccc caataaagt caaactgca 
aactgatct cagcaaacc ggaaaagga gtccaaaaa 
cggaaggtc tgataacca gccgcattc cgcctgatc 
tgtgtaact tatttatta tgattaatt taatttttt 
acgtggtag tttggtgga ctaatacag tacttaatg 
attcaatta atccatcat tccagtagg aattaaaaa 
ctaactaaa attaggctg tgtgagttg tcagctttg 
aatgtctct aaataaaaa att

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY